A facile strategy to prepare porphyrin functionalized ZnS nanoparticles and their peroxidase-like catalytic activity for colorimetric sensor of hydrogen peroxide and glucose

Publication date: November 2017
Source:Sensors and Actuators B: Chemical, Volume 251
Author(s): Qingyun Liu, Pengpeng Chen, Zhe Xu, Miaomiao Chen, Yanan Ding, Kang Yue, Jian Xu
In this article, we demonstrated a facile method to prepare the porphyrin [5,10,15,20-Tetrakis (4-carboxyl phenyl)porphyrin] functionalized ZnS nanoparticles (H2TCPP-ZnS nanocomposites) under the mild conditions. Porphyrin molecules serves as both a functional molecule and stabilizer. TEM images showed that H2TCPP-ZnS nanocomposites are composed of nanoparticles with ca. 5–8nm in size. The nanocomposites were for the first time developed as a peroxidase mimetic with catalytic activities and could catalyze the colorimetric reaction of H2O2 and 3,3′,5,5′-tetramethylbenzidine (TMB), where the oxidized TMB showed a typical absorption peak at 652nm. Kinetic analysis indicated that the catalytic behavior was in accordance with typical Michaelis–Menten kinetics. Moreover, H2TCPP-ZnS nanocomposites could accelerate H2O2 to decompose into ·OH radicals. The intrinsic peroxidase-like activity of H2TCPP-ZnS nanocomposites is due to the synergistic effect of H2TCPP as well as ZnS in the nanocomposites. Based on these findings, a simple and sensitive colorimetric assay to detect H2O2 and glucose with a range of concentration from 0.01 to 0.06mM and from 0.005 to 0.5mM, respectively. In addition, this nanosensor can also be used for glucose detection in diluted serum.

Graphical abstract

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