Synthesis of 1,2-phenylenediamine capturing molecule for the detection of diacetyl

Publication date: Available online 6 October 2017
Source:Data in Brief
Author(s): Lucia Marri, Anita M. Jansson, Caspar E. Christensen, Ole Hindsgaul
Here we describe the design of 1,2-phenylenediamine capturing molecule and the synthesis steps necessary for its preparation. The designed 1,2-phenylenediamine derivative is able to capture diacetyl in solution, as shown by ESIMS, forming a chemical adduct, 1–4-quinoxaline. The methyl esters of diacetyl-adduct (DAA) and pentanedione-adduct (PDA) are incorporated to the lysines in BSA and the conjugate used for antibody screening and selection. In the research article is described an enzyme-linked immunosorbent assay developed to detect and quantify diacetyl in complex media.



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