Necrosulfonamide Attenuates the Spinal Cord Injury Via Necroptosis Inhibition.

Necrosulfonamide Attenuates the Spinal Cord Injury Via Necroptosis Inhibition.

World Neurosurg. 2018 Mar 31;:

Authors: Wang Y, Wang J, Wang H, Feng X, Tao Y, Yang J, Cai J

Abstract
Spinal cord injury (SCI) is a serious trauma without efficient treatment currently. Necroptosis can be blocked post injury by special inhibitors. To investigate the effects of Necrosulfonamide (NSA) in the treatment of SCI, pathological condition, necroptosis related factors, mitochondrial function and ethological performance were detected. Pathological detection using HE staining was performed first. Reduced lesions and protected neurons were found in the injured spinal cord after treatment with NSA. No obvious toxicity on rat liver, kidney, heart and spleen was detected using HE staining. Rather than RIP1 and RIP3, MLKL was significantly inhibited by the NSA using Western blot detection. ATP generation was obviously decreased post injury, but slightly increased after the NSA treatment, especially 24h post injury. No significant changes were found on activities of SOD and caspase-3 after the treatment of NSA. Ethological performance was significantly improved using 21 point open field locomotion test. Our research indicates NSA might be a potential and safe chemical benefit for SCI therapy. To our knowledge, this is the first study on the effects of NSA as treatment of traumatic SCI.

PMID: 29614353 [PubMed - as supplied by publisher]

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