Novel functionalized nanomaterials for the effective enrichment of proteins and peptides with post-translational modifications

Publication date: Available online 17 April 2018
Source:Journal of Proteomics
Author(s): Cristina Núñez, María del Pilar Chantada, Susana B. Bravo, Sergio Vázquez Estévez
Low abundance proteins/peptides usually suffer strong interference with highly abundant proteins/peptides as well as other contaminants, resulting in low ionization efficiency in MS analysis. Hence, the enrichment and separation of proteins/peptides from complex mixtures are of great value to their successful identification.In this review we present an overview of the application of different nanocomposites for the specific enrichment of peptides/proteins with post-translational modifications (PTMs), such as phosphorylation and glycosylation. Furthermore, examples of the selective enrichment of cysteine-containing peptides, histidine-tagged proteins and the isolation of N-terminal peptides will be analyzed.

Graphical abstract

https://ift.tt/2H6feSF