Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

Αρχειοθήκη ιστολογίου

! # Ola via Alexandros G.Sfakianakis on Inoreader

Η λίστα ιστολογίων μου

Τετάρτη 19 Σεπτεμβρίου 2018

The anti-inflammatory effect of milk and dairy products on periodontal cells: an in vitro approach

Abstract

Objective

Milk can reduce intestinal tissue damage in colitis models, and protects infants against necrotizing enterocolitis. However, whether milk can decrease inflammation related to peri-implantitis and oral mucosal dehiscence remains unclear. We therefore investigated whether or not milk and fermented by-products have any anti-inflammatory effects on the cells of the oral cavity.

Material and methods

Human gingival fibroblasts and the human oral epithelial cell line HSC2 were exposed to pasteurized human milk, pasteurized cow's milk, dairy products, and powdered milk. An inflammatory response was then provoked with IL1 and TNFα. The expression changes of IL1, IL6, and IL8 were detected by reverse transcriptase PCR and immunoassay.

Results

We can report that pasteurized human milk and pasteurized cow's milk as well as yoghurt, buttermilk, sour milk, whey, and powdered milk can lower the expression of inflammatory cytokines in gingival fibroblasts being stimulated by IL1 and TNFα. A similar anti-inflammatory response to pasteurized milk and dairy products was observed with the human oral epithelial cell line HSC2.

Conclusion

These data suggest that pasteurized and powdered milk, as well as fermented dairy products, display an anti-inflammatory effect on oral fibroblasts and oral epithelial cells.

Clinical relevance

Our in vitro findings provide the scientific basis to extend this research towards testing the anti-inflammatory effects of milk in a pre-clinical periodontitis and peri-implantitis model.



https://ift.tt/2xnx1Ok

An unusual case of extensive truncal cutaneous larva migrans in a Cameroonian baby: a case report

Cutaneous larva migrans is a neglected zoonotic helminthic disease which is paradoxically underreported in low-income and middle-income countries from where a majority of the cases emanate. It presents as migr...

https://ift.tt/2xABrk7

A Conversation with Jose Gomez Marquez, Little Devices Lab, MIT

3D Printing and Additive Manufacturing, Volume 5, Issue 3, Page 181-183, September 2018.


https://ift.tt/2DeQshA

High-Efficiency High-Resolution Multimaterial Fabrication for Digital Light Processing-Based Three-Dimensional Printing

3D Printing and Additive Manufacturing, Volume 5, Issue 3, Page 185-193, September 2018.


https://ift.tt/2NnNvQg

Patients with Benign Thyroid Disorders Report Impairments in Sex Life

Clinical Thyroidology, Volume 30, Issue 9, Page 405-407, September 2018.


https://ift.tt/2Daqw6C

Needle Biopsy of Thyroid Nodules Is Best Performed Using Capillary Action Techniques Rather than Suction

Clinical Thyroidology, Volume 30, Issue 9, Page 418-421, September 2018.


https://ift.tt/2NtAG7n

Therapy-Induced Hypothyroidism Reduces Long-Term Post-treatment Cardiovascular Morbidity and Mortality in Graves’ Disease and Toxic Multinodular Goiter

Clinical Thyroidology, Volume 30, Issue 9, Page 408-411, September 2018.


https://ift.tt/2DdmOcm

If High Circulating Levels of Biotin Are Commonly Affecting Thyroid-Function Tests, Shouldn't Something Be Done About It?

Clinical Thyroidology, Volume 30, Issue 9, Page 401-404, September 2018.


https://ift.tt/2Nrz7Xa

Some Patients Do Not Undergo Subsequent Evaluation Following an Abnormally Suppressed TSH

Clinical Thyroidology, Volume 30, Issue 9, Page 412-414, September 2018.


https://ift.tt/2DeZhIc

Integrating AJCC-TNM, ATA-IRS, and Patient Age Improves Survival Predictions for Thyroid Cancer

Clinical Thyroidology, Volume 30, Issue 9, Page 422-425, September 2018.


https://ift.tt/2Npy3TH

Quantitative Uptake of Sestamibi Differentiates Type 1 from Type 2 Amiodarone-Induced Thyrotoxicosis

Clinical Thyroidology, Volume 30, Issue 9, Page 415-417, September 2018.


https://ift.tt/2DcVwTr

Is Survival the Optimal End Point for Determining Appropriate Extent of Surgery in Medullary Thyroid Cancer?

Clinical Thyroidology, Volume 30, Issue 9, Page 429-432, September 2018.


https://ift.tt/2NsgW3S

Tumor Regression and Cure Depends on Sustained Th1 Responses

imageWhile immunomodulatory monoclonal antibodies (mAbs) have therapeutic efficacy against many tumors, few patients are cured. Attempting to improve their therapeutic efficacy we have applied the TC1 mouse lung carcinoma model and injected established subcutaneous tumors intratumorally with 3 weekly doses of various combinations of mAbs. Combinations of mAbs to CTLA4/PD1/CD137 (the 3 mAb combination) and to CTLA4/PD1/CD137/CD19 (the 4 mAb combination) were most efficacious to induce complete regression of both the injected tumor and an untreated tumor in the same mouse. Tumor cure was consistently associated with shifting a Th2 to a Th1 response in tumor-draining lymph nodes and spleen and it involved epitope specific and long-lived memory T cells as well as M1 macrophages. This shift and accompanying tumor rejection was harder to achieve as the treated tumors increased in size. Relapse of tumors which had initially regressed following treatment with immunomodulatory mAbs was associated with return of a Th2 microenvironment in tumors, tumor-draining lymph nodes and spleens rather than the emergence of immune-resistant tumor cells. While mAbs to CTLA4 plus PD-1 were therapeutically ineffective, combining the 2 of them with intraperitoneal cisplatin, 10 mg/kg, induced long-term complete tumor regression in most mice with small TC1 tumors and the therapeutic efficacy against larger tumors improved by administrating cisplatin together with the 3 or 4 mAb combination.

https://ift.tt/2QKQ1OP

Understanding Microbiome Effect on Immune Checkpoint Inhibition in Lung Cancer: Placing the Puzzle Pieces Together

Over the past couple of years, human microbiome has received increasing attention as a regulator and predictor of response to the therapies of various diseases. It is speculated that manipulating gut microbiome can modify response to cancer immunotherapies as well. Through this review, we have critically analyzed our current understanding of gut microbiome as a modulator of immunotherapies in lung cancer, explained conflicting data, evaluated current gaps and extrapolated our present knowledge to generate directions for future investigations.

https://ift.tt/2xACIrq

Cancer Stem Cell Vaccination With PD-L1 and CTLA-4 Blockades Enhances the Eradication of Melanoma Stem Cells in a Mouse Tumor Model

imageImmune checkpoint inhibitors and monoclonal antibodies reinvigorate cancer immunotherapy. However, these immunotherapies only benefit a subset of patients. We previously reported that ALDHhigh tumor cells were highly enriched for cancer stem cells (CSCs), and ALDHhigh CSC lysate-pulsed dendritic cell (CSC-DC) vaccine was shown to induce CSC-specific cytotoxic T lymphocytes. In this study, we investigated the CSC targeting effect of the CSC-DC vaccine combined with a dual blockade of programmed death-ligand 1 and cytotoxic T-lymphocyte-associated protein (CTLA-4) in B16-F10 murine melanoma tumor model. Our data showed that animals treated with the dual blockade of programmed death-ligand 1 and CTLA-4 and CSC-DC vaccine conferred significantly more tumor regression than the CSC-DC vaccine alone. Importantly, the triple combination treatment dramatically eliminated ALDHhigh CSCs in vivo. We observed that CSC-DC vaccine in combination with anti-PD-L1 and anti-CTLA-4 administration resulted in ∼1.7-fold fewer PD-1+CD8+ T cells and ∼2.5-fold fewer CTLA-4+CD8+ T cells than the populations observed following the CSC-DC vaccination alone. Moreover, significant antitumor effects and dramatically eliminated ALDHhigh CSCs following the triple combination treatment were accompanied by significantly enhanced T-cell expansion, suppressed transforming growth factor β secretion, enhanced IFN-γ secretion, and significantly enhanced host specific CD8+ T-cell response against CSCs. Collectively, these data showed that administration of a-PD-L1 and a-CTLA-4 combined with CSC-DC vaccine may represent an effective immunotherapeutic strategy for cancer patients in clinical.

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Nivolumab Treatment for Cancers in the HIV-infected Population

imageNivolumab is a standard treatment option in several advanced malignancies, but safety and efficacy are still unknown in patients with human immunodeficiency virus (HIV) infection. We describe a case series of people living with HIV (PLWH) receiving nivolumab in the Veterans Health Administration (VA) and report responses and toxicities. We identified all PLWH who received nivolumab at any VA facility since 2000 in the Corporate Data Warehouse (CDW), which provides nationwide research access to VA electronic medical records. We identified 16 HIV-infected nivolumab recipients. The median number of nivolumab doses received was 6 (range, 1–32). Changes in CD4 count during therapy were variable, with 70% (7/10) of patients experiencing increases. Half of PLWH were treated for non–small-cell lung cancer; 2 for Hodgkin lymphoma (HL), 2 for renal cell carcinoma, and 4 for off-label cancers. For non–small-cell lung cancer, 7 patients had evaluable responses. Although 5 of 7 patients immediately progressed, 1 had a partial response and 1 had stable disease, which were both durable. Two of 16 (14%) PLWH had complete responses; both with HL (2/2 HL, 100%). The prevalence of immune-related adverse effects was 40% overall (6/15); 27% (4/15) had pneumonitis. To our knowledge, this is the largest case series reporting outcomes with nivolumab in PLWH. Outcomes were comparable with those seen in studies of HIV-uninfected patients, and particularly interesting for HL. The reason for the high proportions of immune-related adverse effects is unclear, but needs to be confirmed in larger studies.

https://ift.tt/2xxDipV

JESREC score and mucosal eosinophilia can predict endotypes of chronic rhinosinusitis with nasal polyps

Recently, JESREC score and mucosal eosinophil count have been used to diagnose eosinophilic chronic rhinosinusitis (ECRS) in Japan. However, it remains unknown whether the subtypes of CRS diagnosed by these criteria have different endotypes. In the present study, we investigated whether JESREC score and mucosal eosinophil count were appropriate for classification of CRS subgroups into endotypes.

https://ift.tt/2xjXKez

Management of Microsurgical Patients using Intraoperative Unfractionated Heparin and Thromboelastography

10-1055-s-0038-1670683_170358-1.jpg

J reconstr Microsurg
DOI: 10.1055/s-0038-1670683

Background Maintaining optimal coagulation is vital for successful microvascular tissue transfer. The viscoelastic thromboelastography (TEG) is a modern and dynamic method to assess a patient's coagulation status. The aim of this study was to evaluate its diagnostic capabilities of identifying microvascular complications. Methods A retrospective chart review was conducted for the most recent 100 cases of abdominal free flap breast reconstruction of a single surgeon. Patient demographics, medical history, clinical, and operative details were documented. Thrombocyte counts, prothrombin time (PT), activated partial thromboplastin time (aPTT), and various TEG parameters were gathered for preoperative, intraoperative, and two postoperative time points. Results A total of hundred patients were identified, who underwent 172 abdominal-based free flaps for breast reconstruction. TEG was more dynamic compared with PT or aPTT and demonstrated borderline hypocoagulate values intraoperatively upon unfractionated heparin administration and hypercoagulate values postoperatively. In contrast, PT and aPTT demonstrated a continuously hypocoagulable state. Complications included five thrombotic events and three hematomas. The thrombotic cases had much steeper increases of TEG-G between surgery and postoperative day 2 (p = 0.049), while PT and aPTT failed to identify these patients. Of those, two resulted in flap loss (1.2%) that both occurred in patients with abdominal scars from previous surgery. Conclusion The TEG is a useful adjunct for monitoring coagulation status in microsurgical breast reconstruction. When thrombosis at the anastomosis occurs, TEG correlates with a more rapid rebound from an intraoperative hypocoagulable state to a postoperative hypercoagulable state, when using the TEG. The TEG is a valuable tool for a more dynamic assessment of the patients' changing coagulation status.
[...]

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Article in Thieme eJournals:
Table of contents  |  Abstract  |  Full text



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Radiation-Induced Skin Changes after Postmastectomy Radiation Therapy: A Pilot Study on Indicators for Timing of Delayed Breast Reconstruction

10-1055-s-0038-1670650_180117-1.jpg

J reconstr Microsurg
DOI: 10.1055/s-0038-1670650

Background Delayed autologous breast reconstruction is commonly recommended in patients requiring postmastectomy radiation. This study examines gross and histologic changes in the breast skin of patients who have undergone postmastectomy radiation to help determine when radiation-induced skin changes begin to stabilize. Methods A prospective pilot study was conducted on eight patients with invasive breast cancer who required mastectomy and radiotherapy. At the time of mastectomy and 2, 4, 6, 8, and 12 months after completion of radiotherapy, a punch biopsy was taken from the radiated mastectomy skin of each patient. Serial standardized photographs were taken before and after radiotherapy to evaluate the degree of hyperpigmentation and graded by three blinded plastic surgeons. Skin biopsies were processed for histologic assessment of inflammation, elastin organization, and vascularity. Results Grading of patient photographs revealed an increase in hyperpigmentation after radiotherapy compared with baseline with a gradual improvement over time. SMAD3 immunostaining demonstrated a trend toward an increase in inflammation over 12 months. The elastin distribution within samples showed an increase in fiber disorganization, thickening, and clumping, with no improvement throughout the study period. The average number of vessels per high powered field decreased steadily through the duration of the study. Conclusions Histologic changes in dermal inflammation, elastin organization, and vascularity do not appear to correspond with the gradual improvement of hyperpigmentation, resulting from postmastectomy radiation. These histologic changes persist beyond the 12 month observation period and will require clinical correlation to determine the potential impact on postoperative outcomes.
[...]

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Article in Thieme eJournals:
Table of contents  |  Abstract  |  Full text



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Effects of Systemic and Local Caffeine on Vessel Diameter, Anastomosis Patency, and Intimal Hyperplasia in the Rat

10-1055-s-0038-1672130_180148-1.jpg

J reconstr Microsurg
DOI: 10.1055/s-0038-1672130

Background The use of caffeine is not recommended prior to elective microsurgery due to its demonstrated negative effects on vessel anastomosis by the presumed sympathomimetic induction of vasoconstriction. In this study, we aimed to elucidate the systemic and local effects of caffeine on vessel diameter, anastomosis patency, and degree of intimal hyperplasia during the healing process. Methods Twenty-five rats were randomly assigned to five groups: (1) negative control, (2) preoperative systemic caffeine, (3) postoperative systemic caffeine, (4) perioperative systemic caffeine, and (5) a local caffeine group. Both the right and left femoral arteries were used. Ten anastomoses were performed per group. The arterial diameter was measured by micrometer, anastomosis patency was assessed surgically and histologically, and the histological examination was conducted 3 weeks postoperatively to determine intimal hyperplasia. Results The overall patency rate was 96%. Mild vasoconstriction was observed in the systemic caffeine groups (statistically insignificant); however, there were no negative effects on anastomosis patency. Local caffeine irrigation resulted in significant vasodilatation in the local caffeine group (p = 0.001); a similar effect was not observed in the other groups. There was a significant decrease in the intima/media ratio in the local caffeine group (p < 0.01), when compared with the control and systemic caffeine groups. No other intima/media ratio differences were observed among other comparison groups. Conclusion The systemic administration of caffeine, although statistically insignificant, has an observable effect on vasoconstriction. However, it does not appear to have negative effects on anastomosis patency regardless of its application period (pre-, post-, or perioperatively). The local application of caffeine resulted in considerable vasodilatation as opposed to the vasoconstriction effect in the systemic caffeine groups. Decreased intimal hyperplasia at the anastomosis edge, and antifibrotic properties in the surgical field were also observed in this group. Histologically, the local caffeine group demonstrated an additional beneficial effect on anastomosis remodeling.
[...]

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Article in Thieme eJournals:
Table of contents  |  Abstract  |  Full text



https://ift.tt/2pl3Cjc

Platelet rich plasma as an adjunctive treatment in Lichen Plano pilaris



https://ift.tt/2OAQzFi

A Provider Global Assessment Quality Measure for Clinical Practice for Inflammatory Skin Disorders

Current quality measures in dermatology have limited feasibility and validity for demonstrating value of care provided. Expert consensus was reached on a global severity metric for incorporation into a quality measure for inflammatory dermatoses. This effort will facilitate standardized data collection and may better demonstrate quality of care in dermatology.

https://ift.tt/2NUM1wA

Clinical and dermoscopic features of cutaneous BAP1 inactivated melanocytic tumors: results of a multicenter case-control study by the International Dermoscopy Society (IDS)

Multiple BAP-1 inactivated melanocytic tumors (BIMTs) have been associated with a familial cancer-syndrome involving germline mutations in BAP1. We have identified 5 dermoscopic patterns present in BIMT. Dome-shaped papules with pink-to-tan structureless areas and peripheral irregular dots/globules or network should raise suspicion for BIMT associated with BAP1 germline mutations.

https://ift.tt/2OAQwJC

Clinical outcomes in high-risk squamous cell carcinoma patients treated with Mohs micrographic surgery alone

Various factors are linked to poor outcomes in squamous cell carcinoma, but poor differentiation and invasion beyond subcutaneous fat may greater predict risk of locoregional disease and disease-specific death., Mohs surgery alone provides excellent marginal control of high-risk squamous cell carcinoma and may lower rates of measured poor outcomes.

https://ift.tt/2NTKQNQ

Comment on Okhovat et al “The first 30 years of the American Academy of Dermatology skin cancer screening program: 1985-2014”



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Lack of an FDA indication should not limit access to appropriate treatment



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Dermoscopy of Pityrosporum Folliculitis



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Reflectance confocal microscopy as novel tool for pre-surgical identification of basal cell carcinoma biopsy site



https://ift.tt/2NRr6KP

Rethinking the classification of alopecia areata



https://ift.tt/2OD5BKV

A population-based registry study on relative survival from melanoma in Germany stratified by tumor thickness for each histological subtype

Melanoma relative survival varies by histology. However, differences were strongly alleviated after adjustment for tumor thickness. Increased 5-year relative survival between the periods 2002-2005 and 2010-2013 is explained by a decrease in tumor thickness. Prevention and awareness should be encouraged as prognosis of thick melanoma is still poor.

https://ift.tt/2NY60Kw

Validated Patient-Reported Outcome Measurements for Psoriasis may not Reflect Patients’ Current Preferences



https://ift.tt/2xzUcnN

Comparison of serum 25-hydroxyvitamin D levels between radioimmunoassay and liquid chromatography-tandem mass spectrometry in infants and postpartum women

Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print


https://ift.tt/2pozsvy

Influence of sex on biomarkers of oxidative stress in the kidney, lungs, and liver of rabbits after exposure to diazinon

Abstract

The present study evaluated the effect of two oral doses of the pesticide diazinon (25 and 125 mg/kg bw) on four biochemical parameters (malondialdehyde and glutathione levels, and catalase and glutathione S-transferase enzymatic activities) in the kidney, lungs, and liver of rabbit after 10 days of exposure. Malondialdehyde levels were significantly greater in exposed animals compared to controls, especially in the animals exposed to the higher dose of the pesticide. This result was reflected in the glutathione levels, which were significantly lower at that same higher dose. Catalase activity was also inhibited by the higher dose of the pesticide in all three organs analysed, whereas inhibition of glutathione S-transferase activity was only significant for the kidney and lungs. There were some slight differences between the sexes: taking the results for all three organs, the higher dose of diazinon resulted in a clearly significant inhibitory effect on the catalase activity and glutathione levels in males, and a significant enhancing effect on the malondialdehyde levels in females. These results help to confirm the interest of considering such endogenous factors in future ecotoxicological studies, and that the four biomarkers considered are suitable for reflecting the toxic effects of diazinon on rabbits, especially the effects related to oxidative stress.

Graphical abstract


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Rosacea: 5 Things to Know

These five things include some lesser known, but critically important, facts about rosacea and its management.
Medscape Dermatology

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“Chronic Urticaria”: Recommendations From an Allergist and Immunologist

imageNo abstract available

https://ift.tt/2QJMShW

EMPACT Syndrome in a Patient Treated With Phenobarbital

imageNo abstract available

https://ift.tt/2xC0PWw

Allergic Contact Dermatitis of the Vulva

imageAllergic contact dermatitis is an important diagnostic consideration in the evaluation of patients presenting with vulvar complaints. The high prevalence of contact sensitization in the vulvar region is likely multifactorial. We review the extant literature regarding key predisposing factors in the pathogenesis of vulvar allergic contact dermatitis, as well as the most commonly implicated allergens as identified by a number of retrospective studies and case series. On the basis of our findings, we provide diagnostic and therapeutic recommendations for practicing clinicians.

https://ift.tt/2QJMLD2

Effective Use of Dupilumab in Managing Systemic Allergic Contact Dermatitis

imageAllergic contact dermatitis to metals has become increasingly recognized in patients with endovascular implants. The ACD can lead to in-stent restenosis as well as a prominent eczematous reaction overlying the implant, often necessitating its removal. We present a case of refractory allergic contact dermatitis to nickel in a 44-year-old man with numerous endovascular stents and vascular clips. He developed numerous adverse effects of systemic therapy to manage his symptoms including recurrent infections leading to frequent hospitalizations. He was effectively transitioned to dupilumab, a monoclonal antibody against the IL-4α subunit currently approved by the Food and Drug Administration in the management of atopic dermatitis, with an improvement in symptoms and a reduction in infection rate.

https://ift.tt/2xxzHbq

Contact Dermatitis to Carmine

imageCarmine is a widely used "natural" food additive that has been reported to provoke both an immediate hypersensitivity and a delayed systemic response with cutaneous expression. Systemic contact dermatitis describes the hypersensitivity reaction following systemic re-exposure of the inciting allergen in previously sensitized individuals. In individuals with recalcitrant dermatitis and a positive carmine intolerance history and/or patch test, it is important to consider a trial topical and dietary elimination of carmine-associated products and foods.

https://ift.tt/2QIbFmL

What Is Tetrasodium Etidronate and How Is It Related to Formaldehyde?

No abstract available

https://ift.tt/2xzJ4av

Allergy Alert Test for p-Phenylenediamine–Allergic Hair Dye Users

imageBackground Contact dermatitis to hair dyes remains a health concern. Regulations in many countries require consumer self-testing for hair dyes, but no standardized procedure exists. Objective The aim of this study was to develop a self-test protocol for an allergy alert test (AAT) that can elicit a self-noticeable alert signal in p-phenylenediamine (PPD)–allergic consumers. Methods Simulating consumer use conditions (open application for 45 minutes after mixing with a developer), PPD-positive hair dye–allergic subjects and PPD-negative control subjects were tested on the forearm and behind the ear with experimental products containing 0.05%, 0.25%, 0.75%, and 2% PPD. Reactions were self-evaluated by subjects and independently assessed by dermatologists. Conclusions The AAT caused a reaction self-noticeable on the forearm in 90.5% (38/42) and behind the ear in 93% (39/42) of the PPD-positive subjects. This was objectified by a dermatological evaluation. The strength of the AAT response and the number of responding subjects increased with increasing PPD concentrations. Allergy alert test responses were also dependent on the reaction strength of the diagnostic patch test to PPD before the study; in subjects with (+++) patch test reactions, 19 of 19 were positive. All 48 control subjects were negative to the AAT. Therefore, the AAT protocol provides a signal indicative of an allergic reaction in PPD-allergic hair dye consumers.

https://ift.tt/2QIbCr5

SELF-ASSESSMENT

imageNo abstract available

https://ift.tt/2xzJ22n

Excipient and Dose per Unit Area Affect Sensitivity When Patch Testing With Gold Sodium Thiosulfate

imageBackground Dose/area and reading paradigms for gold patch testing are controversial and not standardized worldwide. Objectives The aims of this study were to determine the optimum patch test dose of gold sodium thiosulfate (GST) in a hydrogel (HYD) and to establish GST HYD safety/efficacy and further characterize normal morphology and time course of GST reactions. Methods Twenty gold-allergic patients were patch tested with a dilution series of GST HYD and with GST 2% petrolatum (pet). Furthermore, this previously determined optimal dose was compared with GST 0.5% pet in 19 known-allergic and 216 consecutive subjects. Results The optimal GST HYD dose was 0.075 mg/cm2, not statistically different from GST 2% pet (P = 0.4795). Gold sodium thiosulfate HYD outperformed GST 0.5% pet in both known-allergic subjects (79% vs 63%, P = 0.2482) and consecutive subjects (30% vs 9%, P

https://ift.tt/2QGD8W1

Clinical Profile and Allergens in Pigmented Cosmetic Dermatitis and Allergic Contact Dermatitis to Cosmetics in India

imageBackground Pigmented cosmetic dermatitis (PCD) is frequently encountered in dark-skinned individuals as gradual hyperpigmentation on the face without preceding erythema or itching. Little is known about the allergen profile in PCD. Objectives The aim of the study was to describe the clinical profile and common allergens in PCD and allergic contact dermatitis (ACD) to cosmetics in Delhi. Methods Records of patients suspected of PCD and ACD to cosmetics were analyzed. All patients were patch tested with the Indian standard series, Indian cosmetic and fragrance series, and personal cosmetics and, in relevant cases, hairdresser series. Results One hundred six patients were analyzed. Patch test was positive in 77 cases (72.6%). Cetrimonium, gallate mix, thiomerosal, and skin lightening creams were more frequently positive in cases of PCD (P = 0.019–0.003), whereas p-phenylenediamine, toluene-2,5 diamine sulfate, p-aminophenol, m-aminophenol, and nitro-p-phenylenediamine were predominantly positive in ACD to cosmetics (P

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Transparent Film Dressings for Patch Testing Leads to Better Adhesion and Patient Comfort

imageNo abstract available

https://ift.tt/2xAeL3l

Sleep Disturbance and Sleep-Related Impairment in Adults With Atopic Dermatitis: A Cross-sectional Study

imageBackground Little is known about the impact of sleep disturbances (SD) or sleep-related impairment (SRI) in adults with AD or their relationship with severity of AD and itch and other predictors. Objective The aim of this study was to determine the relationship between AD severity, SD, and SRI. Methods We conducted a prospective online questionnaire-based study of 287 adults with AD, including assessment of AD severity by Patient-Oriented Eczema Measure, self-reported global AD severity, Self-Assessed Eczema Area and Severity Index and visual analog scale–itch, Patient-Reported Outcome Measurement Information System SD and SRI individual items, and T scores. Results Adults with AD commonly endorsed all SD and SRI symptoms examined; only 58 (21.8%) reported having good or very good sleep quality in the past week. However, only a minority of adults with AD endorsed a more profound impact from these individual aspects of SD and SRI in the past week or Patient-Reported Outcome Measurement Information System T scores greater than 55. In particular, SD and SRI were associated with severe or very severe AD (Patient-Oriented Eczema Measure, self-reported severity, visual analog scale–itch, and/or Self-Assessed Eczema Area and Severity Index). Sleep-related impairment was also associated with comorbid hay fever and/or anxiety. Conclusions This study suggests that SD and SRI are common in adults with AD, particularly those with severe diseases. Sleep disturbances and SRI should be considered when assessing burden of AD and therapeutic decisions.

https://ift.tt/2QIbnwb

Sociology of Nickel Allergy

No abstract available

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Pain and Itch Are Dual Burdens in Atopic Dermatitis

imageBackground Despite being widely reported by patients with atopic dermatitis (AD), pain symptoms, unlike itch, have not been widely assessed. Objective The aim of the study was to understand the distinct pain symptoms in patients with AD. Methods Responses from an anonymous questionnaire were collected from our eczema clinic (in-person survey) and collaboration with Global Parents for Eczema Research Group and the National Eczema Association (online survey) to assess skin pain among patients with AD 5 years and older. Eczema Area and Severity Index was measured in the clinic cohort to correlate with pain symptoms. Conclusions In our international cohort of 103 patients with AD, 78% reported concomitant pain and itch. The greatest pain burden occurred on the hands (odds ratio [OR], 0.77), perioral region (OR, 0.74), and toes (OR, 0.7), corresponding to regions with the greatest sensory nerve density. Pain was most commonly described as "burning" and "stinging," particularly when lesions were red, cracked, and dry. Its presence significantly interfered with sleep, leisure activities, and activities of daily living. Among the clinic cohort, we observed a strong Spearman correlation between objective Eczema Area and Severity Index score and subjective skin pain. It is imperative that clinicians understand patients' unique pain burden to best evaluate clinical severity and quality-of-life interference.

https://ift.tt/2QIbhEP

ALLERGY TO SHOES

No abstract available

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Partial mid-portion Achilles tear resulting in substantial improvement in pain and function in an amateur long-distance runner

This case presents symptom resolution for a long-distance runner with chronic Achilles tendinopathy (AT), following a partial tear of his Achilles tendon. The patient reported a sudden pain during a morning run, with preserved function. Three hours postinjury, he was reviewed in a musculoskeletal clinic. An ultrasound scan confirmed a partial Achilles tear, associated with significant Doppler activity. His index of AT severity The Victorian Institute of Sports Assessment - Achilles Questionnaire (VISA-A) 4 hours postinjury was markedly higher compared with 2 weeks preinjury, indicating reduced symptom severity. A follow-up scan 4 weeks postinjury showed minimal mid-portion swelling and no signs of the tear. His VISA-A score showed continued symptom improvement. This case represents resolution of tendinopathic symptomatology post partial Achilles tear. While the natural histories of AT and Achilles tears remain unknown, this case may indicate that alongside the known role of loading, inflammation may be a secondary mediator central to the successful resolution of AT pain.



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87-year-old woman with improved pulmonary function following accidental long-term inhalation therapy with dabigatran

Accidental long-term dabigatran etexilate inhalation was associated with subtherapeutic dabigatran serum concentrations in an elderly female patient with restrictive lung disease. A significant improvement in her pulmonary function was noted without other therapy directed towards her bronchopulmonary disease.



https://ift.tt/2QJpQI7

Unusual case of levamisole-induced dual-positive ANCA vasculitis and crescentic glomerulonephritis

Cocaine adulterated levamisole is an increasingly reported cause of skin necrosis, arthralgia and systemic vasculitis, but renal involvement is uncommon. We present a case of a 40-year-old Hispanic man with a history of cocaine abuse who presented with acute kidney injury to the rheumatology clinic where he was being treated for chronic inflammatory arthritis. He was found to have a serum creatinine of 2.5 mg/dL, microscopic haematuria and subnephrotic proteinuria, along with positive proteinase 3, myeloperoxidase, anticardiolipin antibodies and an elevated antinuclear antibody titre. The renal pathology revealed focal necrotising glomerulonephritis with crescentic features and mild immune type deposition. The patient was treated with cocaine abstinence, pulse dose steroids followed by maintenance prednisone, rituximab and cyclophosphamide. His renal function subsequently improved but did not normalise. We believe that his incomplete improvement was due to the degree of kidney injury on presentation as well as recidivism with cocaine use.



https://ift.tt/2xwkVl7

Microangiopathic haemolytic anaemia with thrombocytopenia induced by vitamin B12 deficiency long term after gastrectomy

Microangiopathic haemolytic anaemia with thrombocytopenia, called pseudo-thrombotic microangiopathy (TMA), is a clinically important complication in patients with vitamin B12 deficiency. We herein present a case of an 80-year-old woman with pseudo-TMA after gastrectomy. She was initially suspected with thrombotic thrombocytopenic purpura based on rapid progression of anaemia with schistocytes and thrombocytopenia; however, her anaemia and thrombocytopenia were improved by vitamin B12 supplementation alone, with a single session of plasma exchange. Vitamin B12 deficiency was finally confirmed by low vitamin B12 levels from the patient's initial blood sample. In addition, normal ADAMTS13 activity was proven, lowering the likelihood of thrombotic thrombocytopenic purpura. Therefore, this patient was diagnosed with pseudo-TMA caused by vitamin B12 deficiency. Pseudo-TMA can occur in patients with vitamin B12 deficiency post-gastrectomy.



https://ift.tt/2QJpMIn

Herpes simplex virus keratitis mimicking Acanthamoeba keratitis: a clinicopathological correlation

A 36-year-old male, soft contact lens wearer was referred by his primary ophthalmologist for corneal ulcer of the right eye (OD), which was persistent despite topical fluoroquinolone therapy for 1 month. A ring-shaped infiltrate typically seen in Acanthamoeba infection was noted, and topical therapy with chlorhexidine and polyhexamethylene biguanide was initiated. However, the patient's condition deteriorated over the next several weeks; thus, diagnostic and therapeutic penetrating keratoplasty was performed. The postoperative immunohistochemical analysis suggested a diagnosis of herpes simplex virus (HSV) keratitis. The patient ultimately improved after initiation of oral valacyclovir following penetrating keratoplasty. We report a case of a commonly encountered clinical entity, HSV keratitis, with an atypical clinical presentation, masquerading as Acanthamoeba keratitis.



https://ift.tt/2xyjy5j

Acute testicular pain secondary to a leaking abdominal aortic aneurysm (AAA)

We present the case of a 56-year-old male smoker with a background of hypertension who presented with acute onset right testicular pain secondary to a leaking abdominal aortic aneurysm. Following urgent surgical repair and a complicated intensive care recovery, the patient was discharged with no residual disability. This case highlights an atypical presentation of what is a devastating illness.



https://ift.tt/2QJpJwb

Positron emission tomography and reframing vasculitis as a spectrum of disease when investigating a patient with a fever of unknown origin

A retired businessman presented to the infectious diseases department with a history of ongoing fevers and myalgia and raised inflammatory markers. This continued despite adequate antibiotic treatment of an epididymo-orchitis. Extensive investigations, including bone marrow and liver biopsies and a positron emission tomography, did not reveal a cause but showed reactive change in the bone marrow. Later, he developed a vasculitic rash and vision loss due to non-arteritic anterior ischaemic optic neuropathy. High-dose steroids were immediately initiated. A temporal artery biopsy was performed, which confirmed a healing large vessel vasculitis, possibly giant cell arteritis. He has responded very well to therapy. We must better appreciate the limitations of positron emission tomography in investigating a fever of unknown origin. The case also encourages awareness of autoimmune disorders as the leading category of causative diseases for this in older age groups.



https://ift.tt/2QJpGjZ

Rare cause of ovarian mass

Pelvic actinomycosis is a rare entity that occurs almost exclusively in women, the presentation of which is usually non-specific and variable. Pelvic actinomycosis is almost always associated with the use of an intrauterine contraceptive device (IUD). Pelvic actinomycosis unrelated to IUD use is almost always associated with previous surgical procedures. The symptoms, clinical signs and radiological findings are usually non-specific, mimicking an ovarian malignancy. So an awareness of this rare condition and a proper diagnosis can avoid unnecessary surgeries because these cases can be treated with a prolonged course of antibiotics. We present a case of pelvic actinomycosis which masqueraded as an ovarian malignancy.



https://ift.tt/2xwkKGt

Production of gasoline range hydrocarbons from catalytic cracking of linoleic acid over various acidic zeolite catalysts

Abstract

Employment of edible oils as alternative green fuel for vehicles had raised debates on the sustainability of food supply especially in the third-world countries. The non-edible oil obtained from the abundantly available rubber seeds could mitigate this issue and at the same time reduce the environmental impact. Therefore, this paper investigates the catalytic cracking reaction of a model compound named linoleic acid that is enormously present in the rubber seed oil. Batch-scale experiments were conducted using 8.8 mL Inconel batch reactor having a cyclic horizontal swing span of 2 cm with a frequency of 60 cycles per minute at 450 °C under atmospheric condition for 90 min. The performance of HZSM-5, HBeta, HFerrierite, HMordenite and HY catalysts was tested for their efficiency in favouring gasoline range hydrocarbons. The compounds present in the organic liquid product were then analysed using GC-MS and classified based on PIONA which stands for paraffin, isoparaffin, olefin, naphthenes and aromatics respectively. The results obtained show that HZSM-5 catalyst favoured gasoline range hydrocarbons that were rich in aromatics compounds and promoted the production of desired isoparaffin. It also gave a higher cracking activity; however, large gaseous as by-products were produced at the same time.



https://ift.tt/2xBzhk4

Remediation of TCE-contaminated groundwater using KMnO 4 oxidation: laboratory and field-scale studies

Abstract

The objectives of this study were to (1) conduct laboratory bench and column experiments to determine the oxidation kinetics and optimal operational parameters for trichloroethene (TCE)-contaminated groundwater remediation using potassium permanganate (KMnO4) as oxidant and (2) to conduct a pilot-scale study to assess the efficiency of TCE remediation by KMnO4 oxidation. The controlling factors in laboratory studies included soil oxidant demand (SOD), molar ratios of KMnO4 to TCE, KMnO4 decay rate, and molar ratios of Na2HPO4 to KMnO4 for manganese dioxide (MnO2) production control. Results show that a significant amount of KMnO4 was depleted when it was added in a soil/water system due to the existence of natural soil organic matters. The presence of natural organic material in soils can exert a significant oxidant demand thereby reducing the amount of KMnO4 available for the destruction of TCE as well as the overall oxidation rate of TCE. Supplement of higher concentrations of KMnO4 is required in the soil systems with high SOD values. Higher KMnO4 application resulted in more significant H+ and subsequent pH drop. The addition of Na2HPO4 could minimize the amount of produced MnO2 particles and prevent the clogging of soil pores, and TCE oxidation efficiency would not be affected by Na2HPO4. To obtain a complete TCE removal, the amount of KMnO4 used to oxidize TCE needs to be higher than the theoretical molar ratio of KMnO4 to TCE based on the stoichiometry equation. Relatively lower oxidation rates are obtained with lower initial TCE concentrations. The half-life of TCE decreased with increased KMnO4 concentrations. Results from the pilot-scale study indicate that a significant KMnO4 decay occurs after the injection due to the reaction of KMnO4 with soil organic matters, and thus, the amount of KMnO4, which could be transported from the injection point to the downgradient area, would be low. The effective influence zone of the KMnO4 oxidation was limited to the KMnO4 injection area (within a 3-m radius zone). Migration of KMnO4 to farther downgradient area was limited due to the reaction of KMnO4 to natural organic matters. To retain a higher TCE removal efficiency, continuous supplement of high concentrations of KMnO4 is required. The findings would be useful in designing an in situ field-scale ISCO system for TCE-contaminated groundwater remediation using KMnO4 as the oxidant.



https://ift.tt/2QHTFc5

Speciation and uptake of antimony and arsenic by two populations of Pteris vittata L. and Holcus lanatus L. from co-contaminated soil

Abstract

This study aimed to investigate the Sb and As co-accumulating processes of Pteris vittata under soil culture condition, including the transformation of Sb and As, and the difference in co-accumulating ability among different plant species/populations. Two populations of P. vittata and one population of As-tolerant species Holcus lanatus L. were grown on soil co-contaminated by Sb and As. Sb and As speciation in plants was assessed by X-ray absorption near-edge structure (XANES) spectroscopy. P. vittata displayed strong As- but limited Sb-accumulating ability, with the highest shoot concentrations of As and Sb reaching 455 and 26 mg kg−1, respectively. After 28 days culture, the concentrations of Sb and As in the soil solution were reduced by up to 22% and 36% in the P. vittata treatments, respectively. Holcus lanatus showed limited uptake for both metalloids. In P. vittata, the reduction of arsenate to arsenite occurred (with As in shoots all existing as arsenite), but limited reduction of antimonate to antimonite (with more than 90% of Sb in shoots existing as antimonate) was observed. In terms of the differences in metalloid uptake between the two P. vittata populations, the population from the habitat with higher soil As concentration showed 35% higher As uptake than the population from the habitat with lower As concentration. This populational difference may partly result from varying As transformation efficiencies. However, no significant difference was observed in Sb accumulation between the two populations.



https://ift.tt/2xy94D2

Brain-Resident T Cells Following Viral Infection

Viral Immunology, Ahead of Print.


https://ift.tt/2xnmliu

How We Do It: Dermabrasion as a Primary Reconstruction Option for Nasal Defects

No abstract available

https://ift.tt/2OxMOAl

Large Nasal Tip Defects—Utilization of Interdomal Sutures Before Burow's Graft for Optimization of Nasal Contour

No abstract available

https://ift.tt/2NTZgxl

Fire Safety in Mohs Micrographic Surgery

BACKGROUND Surgical fire is a rare event, but one with potentially devastating patient outcomes. OBJECTIVE This study was conducted to investigate the incidence, risk factors, and outcome of surgical fires experienced by members of the American College of Mohs Surgeons (ACMS). METHODS An internet survey was developed and sent to ACMS members. Data collected included total years of experience, total number of cases, typical management of supplemental oxygen, and surgical fires experienced. RESULTS Eighty participants contributed data on 886,200 cases of MMS. Nine surgeons (11%) reported at least 1 surgical fire, yielding an estimated incidence of 1 fire per 88,620 cases (0.001%). The most common site of involvement was the scalp (67%). Common ignition sources included monopolar electrosurgical devices (78%) and battery-powered thermal cautery (22%). Fuel sources included towels or drapes, gauze, isopropyl alcohol, aluminum chloride, hairspray, and diethyl ether. Supplemental oxygen was not involved in any of the cases. Five patients suffered singed hair while 4 patients did not suffer any injuries. None suffered any permanent functional or aesthetic deformities. CONCLUSION The overall risk of surgical fire in MMS is minimal. However, safety measures and greater awareness of fire risks are necessary to prevent patient harm. Address correspondence and reprint requests to: Jeremy T. Kampp, MD, 4225 Roosevelt Way NE, Seattle, WA 98105, or e-mail: jkampp@uw.edu The authors have indicated no significant interest with commercial supporters. © 2018 by the American Society for Dermatologic Surgery, Inc. Published by Wolters Kluwer Health, Inc. All rights reserved.

https://ift.tt/2OGYDog

A Freely Mobile Subcutaneous Nodule of the Dorsal Hand

No abstract available

https://ift.tt/2NTZ94T

Disguising Scars With Decorative Tattooing: A Patient-Inspired Approach

No abstract available

https://ift.tt/2OJF0LW

Commentary on a Retrospective Case-Matched Cost Comparison of Surgical Treatment of Melanoma and Nonmelanoma Skin Cancer in the Outpatient Versus Operating Room Setting

No abstract available

https://ift.tt/2NTZ4y7

Successful Treatment of Lichen Amyloidosis With Trichloroacetic Acid Peels

No abstract available

https://ift.tt/2OCH8Fi

Personal (Self) Perceptions of Submental Fat Among Adults in the United States

BACKGROUND Satisfaction with discrete facial areas influences self-perceptions of attractiveness, self-esteem, and quality of life. Currently, there is a lack of understanding of how the submental area impacts feelings and behaviors. OBJECTIVE To characterize the effects of submental fat (SMF) on feelings/emotions and actions/behavior among adults in the United States. METHODS Online health-based surveys recruited approximately 400 adults (18–65 years) in each of 5 categories based on the respondent's assessment of their SMF. Respondents either agreed or disagreed with 17 statements regarding their feelings/emotions and actions/behaviors related to the area underneath their chin. RESULTS Overall, 1996 respondents were included (equal distribution of males/females; mean age, 41.9 years). Even a slight amount of chin fat was associated with negative feelings and behaviors. As SMF increased, so did the number of respondents reporting negative self-perceptions such as being embarrassed by the area under their chin. In general, a greater percentage of female compared with male respondents reported negative feelings and behavioral changes due to their submental area. CONCLUSION Excess SMF can have a substantial negative effect on a person's feelings of attractiveness and behaviors. Reduction of SMF may not only improve one's appearance, but also may enhance one's self-esteem. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. Address correspondence and reprint requests to: Leslie Baumann, MD, 4500 Biscayne Boulevard, Suite 101, Miami, FL 33137, or e-mail: lsb@derm.net Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (https://ift.tt/2bw6lgr). This study was sponsored by Allergan plc, Dublin, Ireland. Writing and editorial assistance was provided to the authors by Evidence Scientific Solutions, Inc., Philadelphia, Pennsylvania, and funded by Allergan plc, Dublin, Ireland. All authors met the ICMJE authorship criteria. Neither honoraria nor payments were made for authorship. L. Baumann has previously received grants from Allergan and currently receives payment for lectures including service on speakers' bureaus from Allergan. S.M. Shridharani is an advisory board member and consultant for Allergan, Galderma, Merz, and Miramar Labs. S. Humphrey currently receives payment for lectures including service on speakers' bureaus from Allergan and serves as a consultant and/or investigator for Allergan, Galderma, Merz, Revance, Evolus, and BonTi. C.J. Gallagher is an employee of Allergan plc. © 2018 by the American Society for Dermatologic Surgery, Inc. Published by Wolters Kluwer Health, Inc. All rights reserved.

https://ift.tt/2NXnVRE

Noninvasive Atrophic Acne Scar Treatment in Asians With a 755-nm Picosecond Laser Using A Diffractive Optic Lens—A Retrospective Photographic Review

BACKGROUND The diffractive lens of the picosecond laser is relatively new, and there are few reports on its efficacy in treating atrophic acne scars, especially in Asian populations. OBJECTIVE Evaluating the efficacy of diffractive lens 755-nm picosecond laser for atrophic acne scar treatment in Asians. PATIENTS AND METHODS Forty-two patients who were treated for facial atrophic acne scars at a private dermatological clinic were enrolled in this retrospective analysis. Mean session count was 4.28. Before and after photographs were assessed by 2 blinded dermatologists, who rated the amount of overall skin quality improvement on a 5-point scale. RESULTS All patients experienced improvements in scar texture and overall skin quality after 2 to 6 sessions, with scores of +1.4, 1.45, 1.7, 1.33, 2.3, and 1.66 points after 2, 3, 4, 5, 6, and >6 treatments, respectively. There were no obvious adverse effects after treatment. The postinflammatory hyperpigmentation (PIH) risk was 4.7% (2 of 42, both spontaneously resolved). CONCLUSION The 755-nm diffractive lens picosecond laser showed good efficacy and low PIH rates when treating atrophic acne scars in darker skin–type patients. In addition to treatment results, additional improvements in overall skin quality and pigmentation make the picosecond laser an effective and desirable treatment option for Asians. Address correspondence and reprint requests to: Peter Hsien-Li Peng, MD, P-Skin Professional Clinic, 103 Tsoying Great Road, Tsoying District, Kaohsiung, 81347, Taiwan, China, or e-mail: pengskin@gmail.com The authors have indicated no significant interest with commercial supporters. © 2018 by the American Society for Dermatologic Surgery, Inc. Published by Wolters Kluwer Health, Inc. All rights reserved.

https://ift.tt/2NTZ2pZ

Evaluation of Possible Effects of a Potassium Channel Modulator on Temporal Processing by Cochlear Implant Listeners

Abstract

Temporal processing by cochlear implant listeners is degraded and is affected by auditory deprivation. The fast-acting Kv3.1 potassium channel is important for sustained temporally accurate firing and is also susceptible to deprivation, the effects of which can be partially restored in animals by the molecule AUT00063. We report the results of a randomised placebo-controlled double-blind study on psychophysical tests of the effects of AUT00063 on temporal processing by CI listeners. The study measured the upper limit of temporal pitch, gap detection, and discrimination of low rates (centred on 120 pps) for monopolar pulse trains presented to an apical electrode. The upper limit was measured using the optimally efficient midpoint comparison (MPC) pitch-ranking procedure; thresholds were obtained for the other two measures using an adaptive procedure. Twelve CI users (MedEl and Cochlear) were tested before and after two periods of AUT00063 or placebo in a within-subject crossover study. No significant differences occurred between post-drug and post-placebo conditions. This absence of effect occurred despite high test-retest reliability for all three measures, obtained by comparing performance on the two baseline visits, and despite the demonstrated sensitivity of the measures to modest changes in temporal processing obtained in other studies from our laboratory. Hence, we have no evidence that AUT00063 improves temporal processing for the doses and patient population employed.



https://ift.tt/2MIuses

Clinical evaluation of non-surgical cleaning modalities on titanium dental implants during maintenance care: a 1-year follow-up on prosthodontic superstructures

Abstract

Objectives

To investigate tissue health around implants with newly attached superstructures over 12 months of preventive maintenance appointments and instrumentation when necessary.

Material and methods

In a randomized, split-mouth study 32 implants (8 participants with 4 implants each) received followed-up care every 3 months after superstructure attachment. Implants and superstructures were randomly assigned to four treatment groups and treated if necessary: (1) titanium curettes (TC), (2) stainless steel ultrasonic tip (PS), (3) erythritol air-polishing powder (EP), or (4) rubber cup polishing (CON). Probing depths (PDs), bleeding on probing (BOP), modified gingival (mucosal) bleeding index (GBI) around implants, and full-mouth Plaque Control Record (PCR) were measured every 3 months. Clinical attachment levels (CALs) and height of keratinized mucosa (KM)/gingival margins (GMs) for implants/teeth and PD, BOP, and GBI for teeth were documented at baseline, 6 months, and 12 months. Matrix metalloproteinase 8 (MMP-8) and periopathogens were measured at baseline and 12 months.

Results

Participants exhibited minimal signs of periodontal inflammation with statistically significant PD improvement (3.0 ± 0.2 to 2.8 ± 0.3 mm; p = 0.022) and overall CAL (4.3 ± 0.8 to 4.0 ± 0.7 mm; p = 0.048) after 1 year. Implants showed no statistically significant differences (p > 0.05) between or within groups at baseline or 12 months for any parameter, except MMP-8 decreased significantly for PS (14.50 ± 17.58 to 4.63 ± 7.56 ng; p = 0.044), and after 12 months, PCR showed a significant difference between TC and PS (p = 0.018).

Conclusions

Treatment was necessary as inflammation was observed around newly placed superstructures within the first year of maintenance care. All tested treatment modalities yielded comparable clinical improvements.

Clinical relevance

Early assessment and diagnosis of mucositis and regular maintenance can promote long-lasting implant health.



https://ift.tt/2plLMMU

Severe Bronchiolitis Profiles and Risk of Developing Recurrent Wheezing by Age 3 Years

Publication date: Available online 18 September 2018

Source: Journal of Allergy and Clinical Immunology

Author(s): Orianne Dumas, Kohei Hasegawa, Jonathan M. Mansbach, Ashley F. Sullivan, Pedro A. Piedra, Carlos A. Camargo

Abstract
Background

A better understanding of bronchiolitis heterogeneity may help clarify its relationship with the development of recurrent wheezing and asthma.

Objectives

To identify severe bronchiolitis profiles by a clustering approach, and to investigate for the first time their association with allergy/inflammatory biomarkers; nasopharyngeal microbiota; and development of recurrent wheezing by age 3 years.

Methods

We analyzed data from a prospective, 17-center U.S. cohort study of 921 infants (age <1 year) hospitalized with bronchiolitis (2011-2014 winters) with post-hospitalization follow-up. Severe bronchiolitis profiles at baseline (hospitalization) were determined by latent class analysis, based on clinical factors and viral etiology. Blood biomarkers and nasopharyngeal microbiota profiles were determined using samples collected within 24h of hospitalization. Recurrent wheezing by age 3 years was defined based on parental report of breathing problem episodes post-discharge.

Results

Three severe bronchiolitis profiles were identified: profile A (15%), characterized by history of breathing problems/eczema during infancy and non-RSV (mostly rhinovirus) infection; profile B (49%) with the largest probability of RSV infection and which resembled classic RSV-bronchiolitis; and profile C (36%), the most severely ill group. Profile A infants had higher eosinophil counts, higher cathelicidin levels, and elevated proportions of Haemophilus-dominant or Moraxella-dominant microbiota profile. Compared to profile B, we observed significantly increased risk of developing recurrent wheezing in children with profile A (hazard ratio 2.64; 95% CI 1.90-3.68), and, to a lesser extent, with profile C (1.51; 1.14-2.01).

Conclusion

Although longer follow-up is needed, our results may help identify, among children hospitalized for bronchiolitis, subgroups with particularly elevated risk of developing asthma.



https://ift.tt/2xzBKM4

Abstracts

Thyroid, Ahead of Print.


https://ift.tt/2xwLYwB

Abstract Author Index

Thyroid, Ahead of Print.


https://ift.tt/2QGsK0l

Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma

Abstract

The treatment of squamous cell carcinoma (SCC) involves surgery, chemotherapy, and/or radiotherapy, which can cause mucositis (inflammation of the oral mucosa that causes considerable pain and can compromise the continuity of oncological treatment). Photobiomodulation (PBM) has been successfully used in the treatment of mucositis, but doubts arise regarding the use of laser for areas in which tumor cells may remain. In this study, the effect of PBM on the viability, mitochondrial activity, proliferation, apoptosis, and migration of cells derived from oral SCC was evaluated. SCC9 cells were irradiated with laser (660 and 780 nm, using 11 dosimetric parameters) and submitted to mitochondrial and caspase 3 activity tests after 1 and 3 days. Based on the results, cell viability (neutral red assay), proliferation (BrdU assay), and migration (scratch-wound assay) were evaluated using only the dosimetric parameters recommended for mucositis. Non-irradiated cells served as the control. The experiments were performed in triplicate. The 11 parameters diminished mitochondrial activity and induced tumor cell apoptosis. Using the parameters recommended for mucositis, irradiation with 780 nm (70 mW, 4 J/cm2) proved to be the safest and led to a reduction in cell viability, the induction of apoptosis, and a reduction in the migration capacity of the tumor cells.



https://ift.tt/2MMywKt

Evaluation of Possible Effects of a Potassium Channel Modulator on Temporal Processing by Cochlear Implant Listeners

Abstract

Temporal processing by cochlear implant listeners is degraded and is affected by auditory deprivation. The fast-acting Kv3.1 potassium channel is important for sustained temporally accurate firing and is also susceptible to deprivation, the effects of which can be partially restored in animals by the molecule AUT00063. We report the results of a randomised placebo-controlled double-blind study on psychophysical tests of the effects of AUT00063 on temporal processing by CI listeners. The study measured the upper limit of temporal pitch, gap detection, and discrimination of low rates (centred on 120 pps) for monopolar pulse trains presented to an apical electrode. The upper limit was measured using the optimally efficient midpoint comparison (MPC) pitch-ranking procedure; thresholds were obtained for the other two measures using an adaptive procedure. Twelve CI users (MedEl and Cochlear) were tested before and after two periods of AUT00063 or placebo in a within-subject crossover study. No significant differences occurred between post-drug and post-placebo conditions. This absence of effect occurred despite high test-retest reliability for all three measures, obtained by comparing performance on the two baseline visits, and despite the demonstrated sensitivity of the measures to modest changes in temporal processing obtained in other studies from our laboratory. Hence, we have no evidence that AUT00063 improves temporal processing for the doses and patient population employed.



https://ift.tt/2MIuses

Management of Complex Arteriovenous Malformations Using a Novel Combination Therapeutic Algorithm

This case series assesses the efficacy and tolerability of sirolimus as an adjuvant therapy for endovascular embolization in the management of complicated extracranial head and neck arteriovenous malformations.

https://ift.tt/2xpldet

Translating Data on Trends in Disease Management to Decisions

Quantifying health care utilization is an important first step in understanding how to efficiently and effectively deliver health care to patients. Actinic keratoses (AKs) are among the most common clinical diagnoses encountered by dermatologists, and treatment aims to reduce patient discomfort and potential for malignant transformation. Given an aging population, disease burden will likely increase, highlighting the need for understanding trends in AK treatment and reimbursement.

https://ift.tt/2PPtpLB

Antiphospholipid Syndrome Following Pembrolizumab Treatment of Stage IIIB Unresectable Melanoma

The case report describes the occurrence of antiphospholipid syndrome following pembrolizumab treatment of stage IIIB unresectable melanoma.

https://ift.tt/2xpkCtf

Use of Actinic Keratosis Destruction in the Medicare Part B Fee-for-Service Population

This billing claims analysis uses the Medicare Part B Physician/Supplier Procedure Summary Master Files and National Summary Data to evaluate trends in the use and costs of actinic keratosis destruction.

https://ift.tt/2PPrI0N

Comparison of hospital based and home based exercise on quality of life, and neck and shoulder function in patients with spinal accessary nerve injury after head and neck cancer surgery

Publication date: November 2018

Source: Oral Oncology, Volume 86

Author(s): Jung Hwa Do, In Jin Yoon, Young Ki Cho, Jun Su Ahn, Jung Kyo Kim, JaeYong Jeon

Abstract
Objectives

The purpose of this study was to compare the effects of hospital-based and home-based exercise programs on quality of life (QOL) and neck and shoulder function of patients who underwent head and neck cancer (HNC) surgery.

Methods

This clinical trial included 40 patients with neck and shoulder dysfunction after HNC. The exercise program included range of motion (ROM) exercises, massage, stretching, and strengthening exercises. Twenty patients who were assigned to the hospital-based exercise group performed physical therapy for 40 min three times a week for four weeks, and the remaining 20 patients were assigned to the home-based group. The European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire (EORTC QLQ-C30), the EORTC Head and Neck Questionnaire (EORTC QLQ-H&N), the Neck and Shoulder Disability Index (NDI), ROM, and numeric rating scale (NRS) were assessed before and after the exercise program. The program consisted of a 10-minute ROM to the neck and shoulder, a 10-minute massage, and 15 min of progressive resistance exercises, followed by a five-minute stretching exercise.

Results

There were statistically significant differences in the changes of neck and shoulder disability index (p < .05). Additionally, there were significant differences in neck extension and rotation ROM and NRS in the hospital-based group compared with the home-based group (p < .05). QOL was not significantly different between the two groups.

Conclusions

Home-based exercise was effective for improving QOL, shoulder function, and pain relief. Hospital-based exercise had better effects on physical function of the neck and shoulder and reduced pain.



https://ift.tt/2pnfK38

Delusional misidentification of inanimate objects, persons, and places after a left orbitofrontal cortex injury

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Miki Tanabe, Michitaka Funayama, Yota Narizuka, Asuka Nakajima, Isamu Matsukawa, Tomoyuki Nakamura



https://ift.tt/2NqoaoM

Beyond decomposition: processing zero-derivations in English visual word recognition

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Linda Wheeldon, Swetlana Schuster, Christos Pliatsikas, Debra Malpass, Aditi Lahiri

Four experiments investigate the effects of covert morphological complexity during visual word recognition. Zero-derivations occur in English in which a change of word class occurs without any change in surface form (e.g., a boat-to boat; to soak-a soak). Boat is object-derived and is a basic noun (N), whereas soak is action-derived and is a basic verb (V). As the suffix {-ing} is only attached to verbs, deriving boating from its base, requires two steps, boat(N)>boat(V)>boating(V), while soaking can be derived in one step from soak(V). Experiments 1 to 3 used masked priming at different prime durations to test matched sets of one and two-step verbs for morphological (soaking-SOAK) and semantic priming (jolting-SOAK). Experiment 4 employed a delayed-priming paradigm in which the full verb forms (soaking and boating) were primed by noun and verb phrases (a soak/to soak, a boat/to boat). In both paradigms, different morphological priming patterns were observed for one-step and two-step verbs, demonstrating that morphological processing cannot be reduced to surface form-based segmentation.



https://ift.tt/2DbwyUx

Reward Sensitivity Predicts Dopaminergic Response in Spatial Neglect

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Korina Li, Paul Bentley, Ajoy Nair, Omid Halse, Gareth Barker, Charlotte Russell, David Soto, Paresh A. Malhotra

Abstract

It has recently been revealed that spatial neglect can be modulated by motivational factors including anticipated monetary reward. A number of dopaminergic agents have been evaluated as treatments for neglect, but the results have been mixed, with no clear anatomical or cognitive predictors of dopaminergic responsiveness. Given that the effects of incentive motivation are mediated by dopaminergic pathways that are variably damaged in stroke, we tested the hypothesis that the modulatory influences of reward and dopaminergic drugs on neglect are themselves related.

We employed a single-dose, double-blind, crossover design to compare the effects of Co-careldopa and placebo on a modified visual cancellation task in patients with neglect secondary to right hemisphere stroke. Whilst confirming that reward improved visual search in this group, we show that dopaminergic stimulation only enhances visual search in the absence of reward. When patients were divided into REWARD-RESPONDERs and REWARD-NON-RESPONDERs, we found an interaction, such that only REWARD-NON-RESPONDERs showed a positive response to reward after receiving Co-careldopa, whereas REWARD-RESPONDERs were not influenced by drug. At a neuroanatomical level, responsiveness to incentive motivation was most associated with intact dorsal striatum.

These findings suggest that dopaminergic modulation of neglect follows an 'inverted U' function, is dependent on integrity of the reward system, and can be measured as a behavioural response to anticipated reward.



https://ift.tt/2Nrqo7t

A home-based prism adaptation training for neglect patients

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Paola Fortis, Roberta Ronchi, Valeria Velardo, Elena Calzolari, Elisabetta Banco, Lorella Algeri, Maria Simonetta Spada, Giuseppe Vallar

Abstract

Spatial neglect is a debilitating disorder frequently observed after damage to the right cerebral hemisphere. Previous investigations have revealed that prism adaptation (PA) therapy can lead to improvements in neglect-related symptoms. In the typical PA protocol patients repeatedly point toward a visual target while wearing prism goggles. A few years ago, a novel PA procedure, involving a variety of more "ecological" visuo-motor activities during adaptation, less repetitive than a sequence of pointings, was introduced by our research group, and shown to be able to improve neglect-related symptoms to the same extent as the standard pointing task. The ecological procedure was easy to administer and pleasant for the patients. In all previous studies, patients were treated by specialized personnel during hospitalization. In the current study, we investigated the effectiveness of the ecological PA method when performed in a home-based setting, with the help of caregivers and family members. Seven right-brain-damaged patients with chronic left spatial neglect underwent a two-week ecological PA treatment, extended, for two extra weeks, in 6 patients, who were available for this additional rehabilitation session. As a control treatment, patients performed the same activities while wearing neutral goggles, before the PA procedure. Two weeks of ecological PA training proved to be able to significantly improve performance in neuropsychological tests (BIT, Cancellation tasks), a neurological scale (NIH), and functional abilities (CBS), when compared to both the baseline and the neutral control treatment, with improvements being maintained over 6 months. The ecological home-based PA training is effective in alleviating signs of spatial neglect. Importantly, this training is affordable, pleasant, and feasible to be performed in the comfort of the patient's home. Easily extendable to larger patient populations and prolonged periods, this method has a real potential to benefit the quality of life of brain-damaged patients with left spatial neglect.



https://ift.tt/2DapIyE

Estimating the statistical significance of spatial maps for multivariate lesion-symptom analysis

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Grigori Yourganov, Julius Fridriksson, Christopher Rorden



https://ift.tt/2NmkK6w

Functional Specialization for Feature-based and Symmetry-based Groupings in Multiple Object Tracking

Publication date: Available online 18 September 2018

Source: Cortex

Author(s): Chundi Wang, Luming Hu, Siyuan Hu, Yangwen Xu, Xuemin Zhang

Abstract

Our vision systems utilize attention to process dynamic visual information everyday. Multiple object tracking (MOT) is widely used for studying sustained attention in dynamic environments. Previous research reported inter-target grouping based on feature similarity (e.g., targets sharing the same color, size, or shape) can facilitate tracking. A recent study also found that symmetric relation among targets in MOT tasks can automatically improve tracking performance, and a greater grouping effect based on symmetry and feature was observed. However, grouping based on multiple feature cues didn't produce a greater grouping effect than grouping based on one feature cue. Why were groupings based on symmetry and feature additive, but two feature-based groupings were not? How stimulus-driven inter-target groupings based on color or symmetry improve the goal-directed target tracking process remains unknown. We hypothesized that symmetry-based and feature-based groupings would involve different cortical and subcortical areas, and color-based and color-shape-based groupings would be manifested in significant activity in the same regions. This study used functional magnetic resonance imaging (fMRI) to identify cortical and subcortical areas involved in feature-based and symmetry-based groupings respectively during tracking. The results of our experiment showed that lateral occipital (LO) cortex is involved in symmetry-based grouping during tracking. Bilateral putamen, temporal parietal junction (TPJ), and frontal eye field (FEF) are involved in the feature-based grouping. Our findings likely indicate that stimulus-driven inter-target symmetry-based grouping and feature-based grouping improve goal-directed target tracking in different ways.



https://ift.tt/2Da3ehq

Japanese guidelines for the management and treatment of generalized pustular psoriasis: The new pathogenesis and treatment of GPP

The Journal of Dermatology, EarlyView.


https://ift.tt/2PMGTrG

Making a difference providing equitable renal care globally

In their report, Treatment of end-stage renal disease with continuous ambulatory peritoneal dialysis in rural Guatemala, Moore et al describe a 42-year old indigenous Maya man who loses his job after being diagnosed with complicated diabetes. He later develops renal failure of unknown etiology. The patient originally presents to a non-profit clinic, then goes to a "higher-level laboratory in the regional capital," and finally to "The National Centre for Chronic Renal Disease (UNAERC)" The patient is given two options for renal replacement therapy, either twice-weekly haemodialysis or continuous ambulatory peritoneal dialysis (CAPD). Due to his limited mobility and finances, the patient chose CAPD as management of his renal failure.

Chronic Kidney Disease (CKD) is a common effect of many non-communicable disease processes, including hypertension, diabetes, and iatrogenic causes. The burden of this disease, "is significant and rising." CKD in general, and end-stage renal disease (ESRD) in particular are illnesses which have profound impact on the family, social, economic and psychological well-being of patients. As described by the authors, the patient's wife "cannot hold a regular job…as primary caregiver…. The patient and his family have subsided on donations…as well as meagre wages…. The patient… worries constantly about the future. His chronic diseases have caused him to feel distress, helplessness and shame." The patient is not alone in feeling this way. While studies in the developing world regarding quality of life on dialysis patients are lacking, those that do exist support the psychosocial detriment that dialysis is to many.[1] According to one patient, "In many ways… dialysis is the end of hope." [2] This reveals the importance of a multidisciplinary approach to renal replacement in the developing world, with a special emphasis on social work and mental health services.

CKD and ESRD also target vulnerable populations. The authors speak convincingly of a "sickness-poverty cycle… he became too sick to work, then too poor to pay for quality healthcare and medications and consequently he became even sicker." The inability of patients to access care and the inequity created by the care delivered can be devastating for patients who do not possess social capital to protect themselves. This can include the indigenous Maya in Guatemala, others of poor socioeconomic status in Latin America, or even undocumented immigrants in a country with a well-developed healthcare system, such as the United States. [3] Comprehensive, universally accessible healthcare is necessary for the protection of these patients, and may help prevent CKD from transitioning to ESRD. The delivery of this care will be one of the challenges of the future as non-communicable diseases continue to increase in the developing world.

BMJ Case Reports invites authors to submit global health case reports that describe the delivery of renal care for vulnerable patients. These cases could focus on:

-Unique models of delivery in the developing world
-Successful interventions for providing access to vulnerable patients worldwide
-Challenges and complications of renal care in low-resource settings

Manuscripts may be submitted by students, physicians, nurses and allied health professionals to BMJ Case Reports at casereports.bmj.com. For more information, review our guidance on how to write a global health case report and look through our online collection.

To read more about renal care globally at BMJ Case Reports, please review:
Acute peritoneal dialysis in a Jehovah's Witness post laparotomy
Renal failure: unusual clinical presentation of an isolated intracranial hydatid cyst

To read more about kidney disease globally from other cited sources, please review:

[1] Awuah KT, Finkelstein SH, Finkelstein FO. Quality of life of chronic kidney disease patients in developing countries. Kidney international supplements. 2013 May 1;3(2):227-9.
[2] Russ AJ, Shim JK, Kaufman SR. "Is there life on dialysis?": time and aging in a clinically sustained existence. Medical anthropology. 2005 Oct 1;24(4):297-324.
[3] Campbell GA, Sanoff S, Rosner MH. Care of the undocumented immigrant in the United States with ESRD. American Journal of Kidney Diseases. 2010 Jan 1;55(1):181-91.

The post Making a difference providing equitable renal care globally appeared first on BMJ Case Reports blog.



https://ift.tt/2QIOHf1

Facial growth direction after surgical intervention to relieve mouth breathing: a systematic review and meta-analysis

Abstract

Objectives

A systematic review was performed to assess the prognosis for facial growth direction documented by mandibular plane inclination and anterior face height in growing subjects who had undergone surgical intervention to relieve mouth breathing (PROSPERO database, registration no. CRD 42013005707).

Methods

PubMed, Scopus, Web of Science, the Cochrane Library and LILACS were searched based on the guidelines of the PRISMA statement. Included were longitudinal studies with mouth-breathing patients who had undergone surgical interventions to relieve their respiratory pattern, with a minimum follow-up of one year.

Results

A total of 1555 studies were identified, whereby only three nonrandomized clinical trials comprising 155 participants met the inclusion criteria. Primary outcome was change between the initial and final measurements of the mandibular plane-SN angle (95% confidence interval [CI] −2.13° [−3.08, −1.18]). Secondary outcomes included changes in total anterior face height (AFH; 95% CI −0.76 mm [−1.91, 0.38]), upper AFH (95% CI 0.09 mm [−0.57, 0.74]), and lower AFH (95% CI 0.06 mm [−0.87, 0.99]). Risk of bias was low for most of bias domains and the quality of evidence across the studies was considered to be very low. The design, the small number of participants, and the absence of blinding generated imprecision.

Conclusions

There is very low evidence that the mandibular growth direction became more horizontal during the first year after surgery to treat mouth breathing. The total anterior facial height decreased, although not always significantly.



https://ift.tt/2pkhA4X

Surgically assisted rapid maxillary expansion in lingual orthodontics – optimizing of coupling and timing

Abstract

Background

Surgically assisted rapid maxillary expansion (SARME) is primarily used in adult orthodontics. In many cases it is followed by further surgery to address further anteroposterior and/or vertical discrepancies. Treatment times in such cases are often long with adult patients usually requesting invisible appliances. Lingual appliances can provide the mechanical control required as well as fulfil the aesthetic demands in such cases. However lingual appliances are usually custom made and indirectly bonded. Due to tooth movement following surgery there is usually a long delay before impressions can be made for customized lingual appliances. This results in a long delay before alignement and leveling can be commenced post-surgery.

Case presentations

Three cases are presented here demonstrating the simultaneous placement of bone anchored expansion devices for surgically assisted rapid maxillary expansion with customized lingual appliances.

Conclusions

The combination of the two procedures allows the alignement and leveling to commence very soon after surgery significantly reducing treatment times. The design of the appliances and the clinical procedures are described and discussed.



https://ift.tt/2DcZzPL

ECOTOX, new questions for terrestrial and aquatic ecotoxicology



https://ift.tt/2ODIpMs

Effective antimicrobial activity of rifabutin against multidrug‐resistant Helicobacter pylori

Helicobacter, EarlyView.


https://ift.tt/2pokaXr

Evaluation of the efficacy of calcium silicate vs. glass ionomer cement indirect pulp capping and restoration assessment criteria: a randomised controlled clinical trial—2-year results

Abstract

Objectives

Assess calcium silicate cement (Biodentine™) vs. glass ionomer cement (Fuji IX™, control) as indirect pulp capping (IPC) materials in patients with reversible pulpitis after a 2-year follow-up. Evaluate the integrity of the overlying resin composite restorations using modified USPHS criteria and FDI criteria. Investigate the sensitivity of the modified USPHS criteria compared to the FDI criteria in the assessment of the restorations.

Materials and methods

Seventy-two restorations (36 Biodentine™, 36 Fuji IX™) were placed randomly in 53 patients. Periapical radiographs were taken at pre-treatment (T0), 12-month (T12), and 24-month (T24) review. Restorations were assessed using the modified USPHS and FDI criteria at T12 and T24.

Results

At 24 months, 15 teeth had failed to maintain vitality (6 Biodentine™, 9 Fuji IX™). Clinical success rate of IPC for both materials was 72% and is related to the intensity of reversible pulpitis symptoms. No difference was found between T12 and T24 in the periapical (PA) radiographs and in the integrity of the resin composite restorations overlying Biodentine™ compared to Fuji IX™. There was no difference in the efficacy of the USPHS criteria compared to the FDI criteria in the assessment of the resin composite restorations.

Conclusions

Biodentine™ and Fuji IX™ were clinically effective when used as IPC materials in teeth with reversible pulpitis at T24. Resin composite restorations overlying both materials performed well at T24. Using the USPHS or FDI criteria is equally efficient at T24; however, longer term follow-up is needed to establish whether there are sensitivity differences between these assessment criteria.

Clinical significance

Teeth with deep carious lesions approaching the pulp and with signs of reversible pulpitis can be treated successfully by indirect pulp capping using either Biodentine™ or Fuji IX™. Using the USPHS or FDI criteria to assess restorations is equally effective at 2 years.

Trial registration

NCT02201641



https://ift.tt/2QISmtl

Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Abstract

Objectives

The goal of this study is to propose a standard protocol of experimental occlusal trauma to evaluate the inflammatory hyperalgesia induced by metallic crowns on orofacial tissues of rats.

Materials and methods

Thirty animals were randomly divided into six groups (n = 5 per group). Detailed methodology on the manufacturing of metallic crowns is described. The inflammatory hyperalgesia induced by occlusal interference was evaluated by intra-articular injection of a low dose of 0.5% formalin (30 μl) or vehicle (saline) into temporomandibular joint, 21 or 28 days after metallic crown cementation. Posteriorly, pro-inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay to assess the effect of occlusal interference on periodontium.

Results

The cementation of metallic crowns with dental anatomy on the lower molar of rats does not show signs of stress and lack of feeding. Metallic crown-induced occlusal trauma results in a temporomandibular joint inflammatory hyperalgesia (P < 0.05: ANOVA, Tukey's test). Otherwise, it was observed that occlusal trauma results in the increase of protein level of pro-inflammatory cytokines TNF-alpha and IL-1beta in the gingival tissues (P < 0.05).

Conclusion

This study demonstrates in detail a methodology of occlusal trauma resulting from the cementation of metallic crowns in the lower molars of rats, mimicking occlusal interferences commonly evaluated in the dental clinic. This methodology makes new studies to better understand the mechanisms involved in the occlusal trauma of orofacial tissues possible.

Clinical relevance

The standardization of an experimental occlusal interference model will allow us to understand the deleterious effect and mechanisms that affect the orofacial tissues.



https://ift.tt/2xsNzU0

Effect of cold plasma on periodontal wound healing—an in vitro study

Abstract

Objectives

Cold atmospheric plasma (CAP), a room temperate ionized gas, seems to be a possible way to enhance tissue recovery. An in vitro study was conducted to investigate the influence of medical CAP on the regenerative capacity of human periodontal ligament (PDL) cells.

Material and methods

Human PDL cells were subjected to CAP at various intensities, distances, and durations. The effects of CAP on a number of specific markers were studied at transcriptional level using real-time PCR. Additionally, an in vitro wound healing assay was applied to PDL cell monolayers either in the presence or absence of CAP by using JuLI™ Br Live Cell Analyzer and software. Finally, cell viability of CAP-treated cells was analyzed by an XTT assay.

Results

CAP treatment enhanced significantly the expression of the cytokines tumor necrosis factor (TNF)α, cyclooxygenase (COX)2, interleukin (IL)-1β, IL-6, IL-8, collagen (COL)1α, and matrix metalloproteinase (MMP)1, as well as the proliferation markers Ki67 and proliferating cell nuclear antigen (PCNA), but downregulated apoptotic markers Apaf1 and p53. Additionally, the in vitro wound healing rate was significantly enhanced after CAP application. Moreover, CAP treatment resulted in a significantly increased cell viability in the XTT assay.

Conclusion

This in vitro study shows that CAP has regulatable effects on markers of periodontal wound healing thereby underlining the potential use of CAP as a benefit treatment strategy.

Clinical relevance

Our study demonstrates the application of CAP in the treatment of oral pathologies suggesting a promising future treatment approach.



https://ift.tt/2QJ2iTS

Perforation of appendiceal adenocarcinoma ex goblet cell carcinoid: a rare case

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Abstract
Goblet cell carcinoid tumor is a rare form of carcinoid tumor of the appendix. It behaves more aggressively than classical carcinoid tumor of the appendix. Thus, special attention must be given to its disease course and treatment. Our case presentation is of a 68-year-old female who presented with abdominal pain. Computed tomography of her abdomen and pelvis showed a perforated appendicitis. She underwent an ileocectomy with pathological report showing a goblet cell carcinoid tumor of the appendix with negative lymph nodes. A subsequent colonoscopy done 5 months later showed no synchronous lesions and a healthy anastomosis. Given the limited amount of data available about goblet cell carcinoid tumors of the appendix, it is important to report all findings in an effort to improve our understanding and treatment approaches of this rare disease.

https://ift.tt/2xn4vw6

The impact of human papillomavirus (HPV) status on functional outcomes and quality of life (QOL) after surgical treatment of oropharyngeal carcinoma with free-flap reconstruction

Abstract

Background

To determine the impact of Human Papillomavirus (HPV) status on speech, swallowing, and quality of life (QOL) outcomes after surgical treatment of oropharyngeal cancer (OPSCC).

Methods

A retrospective review of a prospectively collected database of all patients with OPSCC diagnosed and treated from 1998 to 2009. Speech, swallowing, and quality of life data were gathered at 3 different evaluation points. HPV status was determined using p16 positivity as a surrogate marker. Univariate and multivariate statistical analyses were performed to identify whether p16 status is a significant predictor of functional outcome and QOL.

Results

One hundred twelve patients with OPSCC and known p16 status were treated with primary surgery between 1998 and 2009, with mean age of 56 years. Out of those patients 63 (56%) were p16 positive. Speech intelligibility remained high at 1-year post operation (95.4%). Only 11.5% of the patients required a feeding tube at 1 year after surgery to maintain their daily caloric requirements and the risk of aspiration after surgery was not significant (p = 0.097). There was no statistically or clinically significant difference in speech, swallowing ability, swallowing safety and QOL outcomes between p16-positive and negative OPSCC.

Conclusions

Surgically treated OPSCC patients demonstrate excellent swallowing function and can achieve excellent speech perception. P16 status may not be predictive of functional outcomes or QOL in surgically treated OPSCC.



https://ift.tt/2NVXeNe

The role of CCR5 in directing the mobilization and biological function of CD11b + Gr1 + Ly6C low polymorphonuclear myeloid cells in cancer

Abstract

Bone marrow (BM) cells of the hematopoietic system, also known as BM-derived leukocytes (BMD), are mobilized from the BM to the blood and then colonize tumor sites. These cells then become key players in either promoting or regulating the development and progression of tumors. Among the cells that suppress anti-tumor immunity are regulatory T cells (Tregs), tumor-associated macrophages (TAMS) and myeloid-derived suppressor cells (MDSC). MDSC comprise CD11b+Gr1+Ly6Clow polymorphonuclear myeloid cells (PMN-MDSC), and CD11b+Gr1+Ly6Chigh monocytic myeloid cells (Mo-MDSC). Several studies including ours have identified the CCR2–CCL2 axis as the key driver of the mobilization of monocytic cells from the BM to the blood and later their colonization at the tumor site. The current review focuses on the mechanisms by which PMN-MDSC are mobilized from the BM to the blood and later to the tumor site, and their clinical implications.



https://ift.tt/2pjunEz

Exercise Training Effects on Cognition and Brain Function in Multiple Sclerosis: Project EXACT

Conditions:   Multiple Sclerosis;   Cognitive Impairment
Interventions:   Behavioral: Treadmill Walking Exercise Training;   Behavioral: Stretching-and-Toning Exercise Training
Sponsors:   University of Alabama at Birmingham;   National Institutes of Health (NIH);   Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Not yet recruiting

https://ift.tt/2NplAiT

Diagnostic DNA Methylation Signature in Diagnosing Thyroid Cancer After Fine-Needle Aspiration in Patients With Thyroid Nodules

Condition:   Thyroid Gland Nodule
Intervention:   Other: Diagnostic DNA Methylation Signature
Sponsors:   City of Hope Medical Center;   National Cancer Institute (NCI)
Not yet recruiting

https://ift.tt/2DaBc57

Cervico-vestibular Rehabilitation for Mild Traumatic Brain Injury

Conditions:   Post-Concussion Syndrome;   Mild Traumatic Brain Injury;   Vestibular Disorder;   Whiplash Injuries;   Concussion, Brain
Interventions:   Other: Conventional approach;   Other: Personalized rehabilitation program
Sponsors:   Laval University;   Centre interdisciplinaire de recherche en réadaptation et intégration sociale;   Université du Québec à Trois-Rivières
Not yet recruiting

https://ift.tt/2Nl34rY

A Prospective Randomized Trial of Capecitabine Treatment in Patients With HNSCC

Condition:   Head and Neck Neoplasms
Intervention:   Drug: Capecitabine
Sponsor:   Zhejiang Cancer Hospital
Recruiting

https://ift.tt/2DdCz3g

Oral Dydrogesterone (OD) Versus Micronized Vaginal Progesterone (MVP) for Luteal Phase Support (LPS) in IVF/ICSI

Conditions:   Infertility, Female;   Infertility;   Genital Diseases, Male;   Genital Diseases, Female;   Progesterone;   Dydrogesterone;   Hormones;   Hormones, Hormone Substitutes, and Hormone Antagonists;   Physiological Effects of Drugs;   Progestins
Interventions:   Drug: Dydrogesterone Oral Tablet;   Drug: Micronized progesterone;   Drug: Placebo Dydrogesterone oral tablet;   Drug: Placebo Micronized progesterone
Sponsors:   CRG UZ Brussel;   Universitätsklinikum Hamburg-Eppendorf;   Abbott;   KU Leuven
Not yet recruiting

https://ift.tt/2NqBdXy

Exercise Training Effects on Cognition and Brain Function in Multiple Sclerosis: Project EXACT

Conditions:   Multiple Sclerosis;   Cognitive Impairment
Interventions:   Behavioral: Treadmill Walking Exercise Training;   Behavioral: Stretching-and-Toning Exercise Training
Sponsors:   University of Alabama at Birmingham;   National Institutes of Health (NIH);   Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Not yet recruiting

https://ift.tt/2NplAiT

Diagnostic DNA Methylation Signature in Diagnosing Thyroid Cancer After Fine-Needle Aspiration in Patients With Thyroid Nodules

Condition:   Thyroid Gland Nodule
Intervention:   Other: Diagnostic DNA Methylation Signature
Sponsors:   City of Hope Medical Center;   National Cancer Institute (NCI)
Not yet recruiting

https://ift.tt/2DaBc57

Cervico-vestibular Rehabilitation for Mild Traumatic Brain Injury

Conditions:   Post-Concussion Syndrome;   Mild Traumatic Brain Injury;   Vestibular Disorder;   Whiplash Injuries;   Concussion, Brain
Interventions:   Other: Conventional approach;   Other: Personalized rehabilitation program
Sponsors:   Laval University;   Centre interdisciplinaire de recherche en réadaptation et intégration sociale;   Université du Québec à Trois-Rivières
Not yet recruiting

https://ift.tt/2Nl34rY

A Prospective Randomized Trial of Capecitabine Treatment in Patients With HNSCC

Condition:   Head and Neck Neoplasms
Intervention:   Drug: Capecitabine
Sponsor:   Zhejiang Cancer Hospital
Recruiting

https://ift.tt/2DdCz3g

Oral Dydrogesterone (OD) Versus Micronized Vaginal Progesterone (MVP) for Luteal Phase Support (LPS) in IVF/ICSI

Conditions:   Infertility, Female;   Infertility;   Genital Diseases, Male;   Genital Diseases, Female;   Progesterone;   Dydrogesterone;   Hormones;   Hormones, Hormone Substitutes, and Hormone Antagonists;   Physiological Effects of Drugs;   Progestins
Interventions:   Drug: Dydrogesterone Oral Tablet;   Drug: Micronized progesterone;   Drug: Placebo Dydrogesterone oral tablet;   Drug: Placebo Micronized progesterone
Sponsors:   CRG UZ Brussel;   Universitätsklinikum Hamburg-Eppendorf;   Abbott;   KU Leuven
Not yet recruiting

https://ift.tt/2NqBdXy

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