Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers?

xlomafota13 shared this article with you from Inoreader Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers? Via Journal of Otolaryngology - Head & Neck Surgery The malignancy potential of the laryngeal lesions are one of the major concerns of the surgeons about choosing the treatment options, forming surgical margins, deciding the follow-up periods. Finding a biomark... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Identification of E2F transcription factor 7 as a novel potential biomarker for oral squamous cell carcinoma

xlomafota13 shared this article with you from Inoreader Identification of E2F transcription factor 7 as a novel potential biomarker for oral squamous cell carcinoma Via Head & Face Medicine As a tumor-accelerating transcriptional factor, E2F transcription factor 7 (E2F7) was up-regulated in many forms of cancers. Nevertheless, little has been reported about the impacts of E2F7 on oral squamous ce... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Preparation and validation of cyclodextrin-based excipients for radioiodinated hypericin applied in a targeted cancer radiotherapy

xlomafota13 shared this article with you from Inoreader Preparation and validation of cyclodextrin-based excipients for radioiodinated hypericin applied in a targeted cancer radiotherapy Via radioactive iodine Int J Pharm. 2021 Feb 24:120393. doi: 10.1016/j.ijpharm.2021.120393. Online ahead of print. ABSTRACT BACKGROUND: Iodine-131 labeled hypericin (131I-Hyp) has been utilized as a necrosis-avid theragnostic tracer in a dual targeting pan-anticancer strategy called OncoCiDia. Widespread use of previously-tested solvent dimethyl sulfoxide (DMSO) is limited by safety concerns. To tackle this, the present study was designed to explore a clinically feasible excipient for the form ulation of the hydrophobic 131I-Hyp for intravenous administration. METHOD: Solubility of Hyp in serial solutions of already-approved hydroxypropyl-β-cyclodextrin (HP-β-CD) was evaluated by UVspectrophotometry and 50% HP-β-CD was chosen for further experiments. Two novel HP-β-CD-based formulations of 131I-Hyp were compared with previous DMSO-based formulation, with regards to necrosis-targetability and biodistribution, by magnetic resonance imaging, single-photon emission computed tomography (SPECT), gamma counting, autoradiography, fluorescence microscopy and histopathology. RESULTS: Hyp solubility was enhanced with increasing HP-β-CD concentrations. The radiochemical purity of 131I-Hyp was higher than 90% in all formulations. The necrosis-targetability of 131I-Hyp in the novel formulations was confirmed in vivo by SPECT and in vitro by autoradiography, fluorescence microscopy and histopathology. The plasma clearance of r adioactivity was faster in the novel formulations. CONCLUSION: The novel 131I-Hyp formulations with HP-β-CD could be a suitable pharmaceutical excipient for 131I-Hyp for intravenous administration. PMID:33639227 | DOI:10.1016/j.ijpharm.2021.120393 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Remote medulla ablongata ventral acute subarachnoid hemorrhage following cervical spinal surgery: A case report

xlomafota13 shared this article with you from Inoreader Remote medulla ablongata ventral acute subarachnoid hemorrhage following cervical spinal surgery: A case report Via leak of cerebrospinal fluid Int J Surg Case Rep. 2021 Feb 22;80:105675. doi: 10.1016/j.ijscr.2021.105675. Online ahead of print. ABSTRACT INTRODUCTION: The incidence of remote intracranial hemorrhage (RICH) in patients during spinal surgery is rare and the detailed mechanism remains unclear. PRESENTATION OF CASE: A 55-year-old man had undergone cervical discectomy and fusion at C5-6 and C6-7 due to herniated disc and secondary spinal canal stenosis. He had severe headache 20 h postoperatively and his drain output increased from 100 to 350 mL in the second 10 h after surgery. Computed tomography (CT) and magnetic resonance imaging (MRI) were performed and he was diagnosed with acute subarachnoid hemorrhage in the ventral medulla oblongata. The drainage tube was quickly removed. Infusion of hypertonic saline was used to reduce intracranial pressure and nimodipine prevented vasospasm around the brainstem. The patient made a gradual, satisfactory recovery with conserv ative treatment. DISCUSSION: The most likely pathomechanism leading to RICH is venous bleeding due to rapid leak of a large amount of cerebral spinal fluid (CSF) after spinal surgery. If the patient has a headache or neurological complaints after spinal surgery, immediate imaging is recommended to confirm the diagnosis. Treatment depends on the amount and location of intracranial hemorrhage. CONCLUSION: RICH is a serious but rare complication of spinal surgery and cerebellar hemorrhage is the most common. The most important pathomechanism leading to RICH after spinal surgery is venous bleeding due to rapid leak of a large amount of CSF. Timely CT is necessary to exclude RICH. Treatment of RICH depends on the size of the intracranial hematoma and the patient's symptoms. PMID:33639502 | DOI:10.1016/j.ijscr.2021.105675 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL‐36γ and IL‐37

xlomafota13 shared this article with you from Inoreader Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL‐36γ and IL‐37 Via Experimental Dermatology Abstract Phototherapy with narrow‐band Ultraviolet B (nb‐UVB) is a major therapeutic option in atopic dermatitis (AD), yet knowledge of the early molecular responses to this treatment is lacking. The objective of this study was to map the early transcriptional changes in AD skin in response to nb‐UVB treatment. Adult patients (n = 16) with AD were included in the study and scored with validated scoring tools. AD skin was irradiated with local nb‐UVB on day 0, 2 and 4. Skin biopsies were taken before and after treatment (day 0 and 7) and analysed for genome‐wide modulation of transcription. When examining the early response after three local UVB treatments, gene expression analysis revealed 77 significantly modulated transcripts (30 down‐ and 47 upregulated). Among them were transcripts related to the inflammatory response, melanin synthesis, keratinization and epidermal structure. Interestingly, the pro‐inflammatory cytokine IL‐36γ was reduced after treatm ent, while the anti‐inflammatory cytokine IL‐37 increased after treatment with nb‐UVB. There was also a modulation of several other mediators involved in inflammation, among them defensins and S100 proteins. This is the first study of early transcriptomic changes in AD skin in response to nb‐UVB. We reveal robust modulation of a small group of inflammatory and anti‐inflammatory targets, including the IL‐1 family members IL36γ and IL‐37, which is evident before any detectable changes in skin morphology or immune cell infiltrates. These findings provide important clues to the molecular mechanisms behind the treatment response and shed light on new potential treatment targets. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The Experience of Greece as a Model to Contain COVID-19 Infection Spread

xlomafota13 shared this article with you from Inoreader The Experience of Greece as a Model to Contain COVID-19 Infection Spread Via In vivo (Athens, Greece) In Vivo. 2021 Mar-Apr;35(2):1285-1294. doi: 10.21873/invivo.12380. ABSTRACT The severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) emerged in late 2019 and has caused a pandemic known as corona virus disease 2019 (COVID-19), responsible for the death of more than 2 million people worldwide. The outbreak of COVID-19 has posed an unprecedented threat on human lives and public safety. The aim of this review is to describe key aspects of the bio-pathology of the novel disease, and discuss aspects of its spread, as well as targeted protective strategies that can help shape the outcome of the present and future health crises. Greece is used as a model to inhibit SARS-COV-2 spread, since it is one of the countries with the lowest fatality rates among nations of the European Union (E.U.), following two consecutive waves of COVID-19 pandemic. Furthermore, niche research technological approaches and scientific recommendations that eme rged during the COVID-19 era are discussed. PMID:33622932 | DOI:10.21873/invivo.12380 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Outcome After Radiation Therapy in Canine Intracranial Meningiomas or Gliomas

xlomafota13 shared this article with you from Inoreader Outcome After Radiation Therapy in Canine Intracranial Meningiomas or Gliomas Via In vivo (Athens, Greece) In Vivo. 2021 Mar-Apr;35(2):1117-1123. doi: 10.21873/invivo.12357. ABSTRACT AIM: To characterize a group of dogs diagnosed with meningioma or glioma treated with radiation therapy and assess the clinical impact of diagnosis and radiation protocol on survival time. PATIENTS AND METHODS: Canine patient records from a single veterinary referral hospital, between 2011 and 2015, were searched for intracranial tumour cases treated with radiation therapy, as a sole modality. Thirty-two dogs were included. RESULTS: Median survival times were 524 days [95% confidence interval (CI)=287-677] in total, 512 days (95% CI=101-682) for the glioma group and 536 days (95% CI=249-677) for the meningioma group. No significant difference in survival was detected when using a definitive or a palliative protocol (p=0.130), nor other prognostic factors were found. CONCLUSION: Our results highlight the efficacy of radiation therapy in the tre atment of canine meningioma, as well as glioma, suggesting a change in the current perception of the response of glial tumours to radiation. PMID:33622909 | DOI:10.21873/invivo.12357 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.