Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

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Δευτέρα 18 Απριλίου 2016

Comparison of the Oral Microbiome of Siblings Using Next-generation Sequencing: a Pilot Study

Abstract

Objective

The purpose of this study was to compare the oral microbiome of siblings with and without dental caries using next-generation sequencing.

Materials and Methods

To investigate the oral microbiome composition, 14 young siblings, 7 with caries and 7 without, were enrolled from seven sibling-pair families. Supragingival plaque samples were collected from the cervicobuccal area of posterior teeth. All samples were analyzed by pyrosequencing, based on the 16S rRNA gene hypervariable regions, V1-V4.

Results

The organisms identified belonged to 65 genera. Fifty-two genera were identified in the subjects with caries and 58 in those without; 45 genera were shared by both groups. In the Shannon index, the caries group showed lower bacterial diversity than the caries-free group and the difference was significant (Wilcoxon signed-rank test, p < 0.05). Additionally, similarities between siblings were evident in analyses based on weighted UniFrac distances (p < 0.05).

Conclusions

In this study, the diversity of the microbiome was reduced in subjects with dental caries, while similarity between siblings seemed to be retained.

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