FRET Pairs with Fixed Relative Orientation of Chromophores

Synthetic routes to different oligospirothioketal (OSTK) Förster resonance energy transfer (FRET) constructs are described and the photophysics of these constructs were explored in different solvents. The FRET efficiencies were determined from the experimental data and compared with theoretical values. The influence of the outstanding rigidity of the novel OSTK compounds on the FRET is discussed.

FRET pairs with a fixed relative orientation of chromophores have been synthesized by combining fluorophores with oligospirothioketal (OSTK) rods. Their photophysical properties have been investigated and are described herein.

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