MnO2 nanosheets as an artificial enzyme to mimic oxidase for rapid and sensitive detection of glutathione

Publication date: 15 April 2017
Source:Biosensors and Bioelectronics, Volume 90
Author(s): Jing Liu, Lingjie Meng, Zhaofu Fei, Paul J. Dyson, Xunan Jing, Xing Liu
Nanozymes are increasingly used as components in assays and diagnostics. Here, we describe a rapid and highly sensitive colorimetric assay for the detection and quantification of glutathione (GSH) employing MnO2 nanosheets as an artificial oxidase. In the assay pale yellow 3,3´,5,5´-tetramethylbenzidine (TMB) is oxidized to a blue product (oxTMB) under catalyzing of MnO2 nanosheets with a significant change in absorption at 650nm. GSH selectively inhibits this reaction with a detection limit of 300nM. The high specificity of inhibition by GSH allows this system to be used to determine the GSH concentrations in human serum samples. The MnO2 nanosheet-based assay is simple, rapid, sensitive and selective for the quantification of GSH and surpasses detection methods based on other MnO2 nanomaterials.



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