Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
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alsfakia@gmail.com

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Δευτέρα 26 Δεκεμβρίου 2016

CAMSAP3-dependent microtubule dynamics regulates Golgi assembly in epithelial cells

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Publication date: Available online 26 December 2016
Source:Journal of Genetics and Genomics
Author(s): Jing Wang, Honglin Xu, Yuqiang Jiang, Mikiko Takahashi, Masatoshi Takeichi, Wenxiang Meng
The Golgi assembly pattern varies among cell types. In fibroblastic cells, the Golgi apparatus concentrates around the centrosome that radiates microtubules; whereas in epithelial cells, whose microtubules are mainly noncentrosomal, the Golgi apparatus accumulates around the nucleus independently of centrosomes. Little is known about the mechanisms behind such cell type-specific Golgi and microtubule organization. Here, we show that the microtubule minus-end binding protein Nezha/CAMSAP3 plays a role in translocation of Golgi vesicles in epithelial cells. This function of CAMSAP3 (calmodulin-regulated spectrin-associated protein 3) is supported by CG-NAP (centrosome and Golgi localized PKN-associated protein) through their binding. Depletion of either one of these proteins similarly induces fragmentation of Golgi membranes. Furthermore, we find that stathmin-dependent microtubule dynamics is graded along the radial axis of cells with highest activity at the perinuclear region, and inhibition of this gradient disrupts perinuclear distribution of the Golgi apparatus. We propose that the assembly of the Golgi apparatus in epithelial cells is induced by a multi-step process, which includes CAMSAP3-dependent Golgi vesicle clustering and graded microtubule dynamics.



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