A novel dual-ratiometric-response fluorescent probe for SO2/ClO− detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO− induced oxidative stress

Publication date: July 2017
Source:Biomaterials, Volume 133
Author(s): Kun Dou, Qiang Fu, Guang Chen, Fabiao Yu, Yuxia Liu, Ziping Cao, Guoliang Li, Xianen Zhao, Lian Xia, Lingxin Chen, Hua Wang, Jinmao You
Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO2 can be generated as the anti-oxidant, while SO2 is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO2 under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO2 and ClO⁻. The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO2 and ClO⁻, allowing the highly selective and accurate ratiometric quantification for both SO2 and ClO⁻ in cells. Moreover the ultrafast (SO2: <60 s; ClO⁻: within sec) and highly sensitive (detection limits: SO2: 3.5 nM; ClO⁻: 12.5 nM) detection were achieved. With the robust applicability, the developed probe was successfully used to quantify SO2 and endogenous ClO⁻ in respectively the HeLa cells and the RAW 264.7 cells, as well as to visualize the dynamic of SO2/ClO⁻ in zebrafish. The fluorescent imaging studies and flow cytometry analysis confirmed the burst-and-depletion and meanwhile the oxidative-and-antioxidative effects of intracellular SO2 under the NaClO induced oxidative stress.

Graphical abstract

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