Atopic dermatitisIL17A and IFN-γ-producing lymphocytes: investigation in blood, chronic lesions and APT

Abstract

After stimulation, skin and blood-derived mononuclear cells were washed with phosphate buffered saline and stained with anti-CD4 FITC, anti-CD3 PerCP and anti-CD45 APC antibodies, then fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin/1% fetal calf serum, before an additional staining with intracytoplasmic anti-IL17A PE or anti-IFN-γ PE or control isotype IgG1 PE. Cells were acquired with FACSCANTO II and analyzed with FlowJo software.

This article is protected by copyright. All rights reserved.

http://ift.tt/2nLiaGC