Publication date: 15 October 2017
Source:Biosensors and Bioelectronics, Volume 96
Author(s): Juan Xiang, Xiaomei Pi, Xiaoqing Chen, Lei Xiang, Minghui Yang, Hao Ren, Xiaojuan Shen, Ning Qi, Chunyan Deng
For the multi-analyte detection, although the sensitivity has commonly met the practical requirements, the reliability, reproducibility and stability need to be further improved. In this work, two different aptamer probes labeled with redox tags were used as signal probe1 (sP1) and signal probe2 (sP2), which were integrated into one unity DNA architecture to develop the integrated signal probe (ISP). Comparing with the conventional independent signal probes for the simultaneous multi-analyte detection, the proposed ISP was more reproducible and accurate. This can be due to that ISP in one DNA structure can ensure the completely same modification condition and an equal stoichiometric ratio between sP1 and sP2, and furthermore the cross interference between sP1 and sP2 can be successfully prevented by regulating the complementary position of sP1 and sP2. The ISP-based assay system would be a great progress for the dual-analyte detection. Combining with gold nanoparticles (AuNPs) signal amplification, the ISP/AuNPs-based aptasensor for the sensitive dual-analyte detection was explored. Based on DNA structural switching induced by targets binding to aptamer, the simultaneous dual-analyte detection was simply achieved by monitoring the electrochemical responses of methylene blue (MB) and ferrocene (Fc) This proposed detection system possesses such advantages as simplicity in design, easy operation, good reproducibility and accuracy, high sensitivity and selectivity, which indicates the excellent application of this aptasensor in the field of clinical diagnosis or other molecular sensors.
http://ift.tt/2pI0qMq
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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- Role for RIF1-Interacting Partner DDX1 in BLM Recr...
- Five-year outcomes of an oropharynx-directed treat...
- Macrophage polarization differs between apical gra...
- Approach to Severe Cutaneous Adverse Drug Reactions
- Hypoxia-based strategies for regenerative dentistr...
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- Evaluating the predictability of the in vitro tran...
- Neutrophil Extracellular Traps are associated with...
- Intratympanic steroid delivery by an indwelling ca...
- Severely infected pneumoceles of the frontal sinus...
- Mechanisms of Immune Tolerance in Leukemia and Lym...
- Intratympanic steroid delivery by an indwelling ca...
- Severely infected pneumoceles of the frontal sinus...
- Intratympanic steroid delivery by an indwelling ca...
- Severely infected pneumoceles of the frontal sinus...
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- The relationship between social desirability bias ...
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- Densification and crack suppression in selective l...
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