Activity of saponins from Medicago species against HeLa and MCF-7 cell lines and their capacity to potentiate cisplatin effect.

Activity of saponins from Medicago species against HeLa and MCF-7 cell lines and their capacity to potentiate cisplatin effect.

Anticancer Agents Med Chem. 2017 Jul 27;:

Authors: Avato P, Migoni D, Argentieri M, Tava A, Fanizzi FP

Abstract
In the present paper we aim to describe the cytotoxic properties of saponin mixtures from M. arabica (tops and roots), M. arborea (tops) and M. sativa (tops, roots and seeds) and related prosapogenins from M. arborea and M. sativa (tops) against HeLa and MCF-7 cell lines. In addition, cytotoxicity of purified soyasaponin I and some purified saponins (1-8) of hederagenin, medicagenic and zanhic acid is also presented. Saponins from M. arabica tops and roots (mainly monodesmosides of hederagenin and bayogenin) were the most effective to reduce, at certain concentrations, proliferation of HeLA and MCF-7 cell lines, showing potential as anti-cancer agents against HeLa. Among the purified saponins, the most cytotoxic was saponin 1, 3-O-ß-D-glucopyranosyl(1®2)-a-L-arabinopyranosyl hederagenin. When saponins, derived prosapogenins and pure saponins were used in combination with the antitumoral drug cisplatin, they all, to different extent, were able to potentiate cisplatin activity against HeLa cells but not against MCF-7 cell lines. Moreover uptake of cisplatin in these cell lines was significantly reduced. Overall results showed that some specific molecular types of saponins (hederagenin glycosides) from Medicago have potential as anti-cancer agents or as leads for anti-cancer agents. Moreover saponins from Medicago species have evidenced interesting properties to mediate cisplatin effects in tumor cell lines, suggesting further investigations.

PMID: 28748756 [PubMed - as supplied by publisher]

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