Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

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! # Ola via Alexandros G.Sfakianakis on Inoreader

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Τρίτη 4 Ιουλίου 2017

Physicochemical properties, in vitro cytotoxic and genotoxic effects of PM 1.0 and PM 2.5 from Shanghai, China

Abstract

Exposure to ambient particulate matter (PM) links with a variety of respiratory diseases. However, compared with coarse particles (PM10) and fine particles (PM2.5), submicrometer particles (PM1.0) may be a more important indicator of human health risks. In this study, the cytotoxic and genotoxic effects of PM1.0 samples from Shanghai were examined using A549 cells, and compared with the effects of PM2.5, to better understand the health effects of PM1.0 in this area. The PM1.0 and PM2.5 samples were characterized for morphology, water-soluble inorganic ions, organic and elemental carbon, and metal elements. The cytotoxicity of PMs was measured using cell viability and cell membrane damage assays. The genotoxic effects of PMs were determined using the comet assay, and DNA damage was quantified using olive tail moment (OTM) values. The physicochemical characterization indicated that PM1.0 was enriched in carbonaceous elements and hazardous metals (Al, Zn, Pb, Mn, Cu, and V), whereas PM2.5 was more abundant in large, irregular mineral particles. The biological results revealed that both PM1.0 and PM2.5 could induce significant cytotoxicity and genotoxicity in A549 cells, and that exposure to PM1.0 caused more extensive toxic effects than exposure to PM2.5. The greater cytotoxic effects of PM1.0 can be attributed to the combined effects of size and chemical composition, whereas the genotoxic effects of PM1.0 may be mainly associated with chemical species.



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