Immobilization of triclosan and erythrosine in layer-by-layer films applied to inactivation of microorganisms

Publication date: Available online 22 April 2018
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Maria F. Costa Pedro, Alessandro S. Kalck, Kevin F. dos Santos, Marcos S. Sousa, Karla B. Romio, Paula C.S. Souto, Josmary R. Silva, Nara C. de Souza
The use of the layer-by-layer (LbL) deposition technique allows materials, such as drugs, to be self-assembled in multilayers with other electrolytes by combining their properties in a nanostructured system. Triclosan (TCS) is commonly used as a drug because of its bactericidal action, while erythrosine (ERY) has been used as a photosensitizer in photodynamic therapies because of its high light absorptivity in the visible region of the electromagnetic spectrum. The major advantage of investigating systems immobilized in LbL films is the benefit of characterizing the interaction through available substances in solid state techniques. It was possible to immobilize in LbL films, ERY, and ERY + TCS. The results show that the growth of the films was linear, indicating the deposition of the same amount of material from the first bilayer without substrate interference. The release analysis showed slow kinetics, which occurred more rapidly for ERY LbL films, probably due to apparent activation energy, which were higher for films with TCS. The combination of TCS, ERY, and laser light (532 nm) for photodynamic inactivation of the fungus Candida albicans was analyzed, and the results were promising for future studies in applications, such as coating surfaces of dental implants.

Graphical abstract

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