In vivo monitoring of nicotine biosynthesis in tobacco leaves by low-temperature plasma mass spectrometry

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Sandra Martínez-Jarquín, Humberto Herrera-Ubaldo, Stefan de Folter, Robert Winkler
Low-temperature plasma (LTP) is capable of ionizing a broad range of organic molecules at ambient conditions. The coupling of LTP to a mass analyzer delivers chemical profiles from delicate objects. To investigate the suitability of LTP ionization for mass spectrometry (MS) based in vivo studies, we monitored the auxin-regulated nicotine biosynthesis in tobacco (Nicotiana tabacum) and evaluated possible biological effects. The measured nicotine concentrations in different experiments were comparable to literature data obtained with conventional methods. The observed compounds suggest the rupture of trichomes, and cell damage was observed on the spots exposed to LTP. However, the lesions only affected a negligible proportion of the leaf surface area and no systemic reaction was noted. Thus, our study provides the proof-of-concept for measuring the biosynthetic activity of plant surfaces in vivo.

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