Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia.

Related Articles

Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia.

CNS Oncol. 2018 Apr 30;:

Authors: Rosenberg T, Aaberg-Jessen C, Petterson SA, Kristensen BW

Abstract
AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.
MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.
RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.
CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

PMID: 29708435 [PubMed - as supplied by publisher]

https://ift.tt/2w1D6Bw