Publication date: July 2018
Source:Biomedicine & Pharmacotherapy, Volume 103
Author(s): Qiuling Sang, Daju Sun, Zonghan Chen, Weijia Zhao
ObjectiveIt has been reported that sciatic nerve injury (SNI) leads to degeneration, damage, and apoptosis of motor neurons. Nerve growth factor (NGF) plays a pivotal role in regeneration and reestablishment of neuronal function via activating PI3K/Akt survival signaling pathways. Curcumin owns neuroprotective effect following brain injury. In the present study, we attempt to investigate underlying mechanism of neuroprotective effect of curcumin through elucidating its correlation with NGF and PI3K/Akt signaling pathways in vitro and in vivo.MethodsPC-12 cells were exposed H2O2 in order to induce neuron cell injury and cells were then treated with curcumin. Caspase-3, NGF level and Akt phosphorylation were determined using flow cytometry and western blotting. Then, cells were treated with NGF specific siRNA followed by measurement of apoptosis, NGF and Akt phosphorylation levels. In animal model, rats were subjected to SNI and then randomly designated into four different groups: curcumin, curcumin + LY294002, curcumin + NGF shRNA, and negative controls and 12 rats in each group (n = 12). After four weeks of continuous treatment, tissue samples were obtained and subjected to TUNEL, NeuN double staining and western blotting.ResultsCurcumin significantly reduced the number of apoptotic cells induced by H2O2 and this effect was associated with upregulation of TrkA, Akt and downregulation of p17. ProNGF level was significantly decreased while mature NGF level was increased with curcumin treatment. When NGF was suppressed, anti-apoptotic effect of curcumin was attenuated. In addition, inhibition of PI3K/Akt results in increased apoptotic rate compared to vehicles following curcumin treatment which was reflected by decreased p17, Ki67, and cyclin D1. Suppression of NGF and inhibition of PI3K led to increased neuron cell death through increasing proNGF and decreasing mNGF, Akt, TrkA, p75NTR, and p17.ConclusionOur findings revealed that curcumin exerts its protective effect against injured neurons through stimulating NGF release which further activates TrkA and PI3K/Akt cell survival signaling.
https://ift.tt/2jVcF7V
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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NGF and PI3K/Akt signaling participate in the ventral motor neuronal protection of curcumin in sciatic nerve injury rat models
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- Otolaryngology and the Global Burden of Disease
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- Regional Overview of Specific Populations, Workfor...
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- Bed II Sequence Stratigraphic context of EF-HR and...
- Technological behaviour in the early Acheulean of ...
- Editorial Board
- Frank Brown (1943–2017)
- Patterns of lateral enamel growth in Homo naledi a...
- Mesopithecus pentelicus from the Turolian locality...
- Reevaluation of ‘endocostal ossifications’ on the ...
- The effect of ontogeny on estimates of KNM-WT 1500...
- A new tephrochronology for early diverse stone too...
- From the Oldowan to the Acheulean at Olduvai Gorge...
- Was the last common ancestor aping a chimp or just...
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- Pancreatic panniculitis: A case series from a tert...
- A rare case of chronic expanding haematoma in the ...
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