Cell-free microRNA miR-505-3p in graft preservation fluid is an independent predictor of delayed graft function after kidney transplantation

Background Delayed graft function (DGF), a common complication after transplantation of deceased donor kidneys, affects both short- and long-term outcomes. Currently available biomarkers during graft preservation lack sensitivity in predicting risk for DGF. The aim of this study is to identify cell-free microRNA biomarkers in graft preservation fluid predictive of DGF after kidney transplantation. Methods Vascular bed preservation fluid was collected from 48 kidney grafts from DCD or DBD (donation after circulatory death or brain death) donors. MiRNA profiles were determined by PCR array (n=8) and validated by RT-qPCR (n=40). Graft function post-transplantation was defined as immediate good function (IF) or DGF. Results A total of 223 miRNAs fulfilled the pre-set parameters (Ct

https://ift.tt/2RX5yL6