Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
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Κυριακή 18 Νοεμβρίου 2018

The effect of neonatal maternal separation on short-chain fatty acids and airway inflammation in adult asthma mice

Publication date: Available online 17 November 2018

Source: Allergologia et Immunopathologia

Author(s): L. Qian, L. Lu, L. Huang, Q. Wen, J. Xie, W. Jin, H. Li, L. Jiang

Abstract
Background

To investigate neonatal maternal separation (NMS) effects on airway inflammation of asthma and potential mechanism using a mouse model.

Methods

80 Balb/c neonatal male mice were randomly assigned to NMS and non-NMS groups. Feces were collected on PND21, 28, 35 and 42 to analyze microbiota and short-chain fatty acids (SCFAs). Non-NMS group were then divided into control (group A) and asthma groups (group B), while NMS group was assigned to NMS + asthma (group C) and NMS + SCFAs + asthma groups (group D). Inflammatory cells and eosinophils (EOS) in bronchoalveolar lavage fluid (BALF) were assessed. Pathological changes and cytokines in lung tissue were observed. Protein expression of Occludin and E-cadherin in airway epithelial was examined.

Results

The number of S′, diversity index H′ and dominance index D′, as well as content butyric acid in NMS group C were significantly lower than non-NMS group B (p < 0.05). Mice in group C had a higher level of inflammatory cells and EOS compared with group A, B and D. EOS moderate infiltration was found in mice of group B, C and D. Mice in group C had significantly higher levels of cytokines and showed slightly increased bronchial epithelium goblet cells and a small amount of visceral secretions. Occludin and E-cadherin expression in lung in B, C and D groups was depressed, and protein level in group C was significantly lower than group B and D.

Conclusions

NMS is associated with exacerbated inflammation of adult asthma by changing intestinal microflora resulting in butanoic acid decline and airway epithelial barrier damage.



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