Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

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! # Ola via Alexandros G.Sfakianakis on Inoreader

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Κυριακή 3 Φεβρουαρίου 2019

Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens

Abstract

This study aimed to elucidate the response of broiler chickens to the dietary nano-zinc supplementation in terms of immune response and antioxidant activity. Ninety-one-day-old chicks (Ross 308) were randomly assigned to one of three dietary treatments in three replicates, in a feeding trial that lasted for 5 weeks. Birds were fed a basal diet supplemented with inorganic zinc oxide at 40 mg/kg diet (control), zinc oxide nanoparticles (ZnONPs) at 40 mg/kg diet (ZN1), or ZnONPs at 80 mg/kg diet (ZN2). Birds were injected with DNP-KLH at the 7th and 21st days from the beginning of the experiment, and blood samples were collected on days 7, 14, 21, 28, and 35 to determine the levels of immunoglobulin Y (IgY) and malondialdehyde as well as the antioxidant enzyme activities. Cellular immunity was assayed by estimation of phagocytic percentage and index of peripheral monocytes of blood and estimation of the T lymphocyte activity using a lymphocyte transformation test. The results showed that feeding broiler chickens a diet supplemented with ZnONPs increased (p < 0.05) the activity of superoxide dismutase and catalase and decreased the concentration of malondialdehyde compared to the control diet, without significant differences between NZ1 and NZ2 diets. Moreover, the chicks fed diets supplemented with ZnONPs showed a significant increase (p < 0.05) in serum IgY, total lymphocyte count, and macrophages compared to the control. A higher significant response for antibodies IgY concentration was observed in birds fed the NZ2 vs NZ1 diet. Also, there was a significant increase in phagocytic activity and phagocytic index in ZnONP-fed groups with a higher significance in the group fed NZ1 than with NZ2 diet as compared with the control. In conclusion, ZnONP application up to 80 mg/kg in the diet is safe for broiler chickens and could improve their antioxidant defense and cellular immunity.



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