Publication date: Available online 31 July 2017
Source:Vaccine
Author(s): Carrie L. Pierce, Tracie L. Williams, Wanda I. Santana, Marnie Levine, Li-Mei Chen, Hans C. Cooper, Maria I. Solano, Adrian R. Woolfitt, Wayne A. Marasco, He Fang, Ruben O. Donis, John R. Barr
As a result of recent advances in mass spectrometry-based protein quantitation methods, these techniques are now poised to play a critical role in rapid formulation of pandemic influenza vaccines. Analytical techniques that have been developed and validated on seasonal influenza strains can be used to increase the quality and decrease the time required to deliver protective pandemic vaccines to the global population. The emergence of a potentially pandemic avian influenza A (H7N9) virus in March of 2013, prompted the US public health authorities and the vaccine industry to initiate production of a pre-pandemic vaccine for preparedness purposes. To this end, we evaluated the feasibility of using immunocapture isotope dilution mass spectrometry (IC-IDMS) to evaluate the suitability of the underlying monoclonal and polyclonal antibodies (mAbs and pAbs) for their capacity to isolate the H7 hemagglutinin (HA) in this new vaccine for quantification by IDMS. A broad range of H7 capture efficiencies was observed among mAbs tested by IC-IDMS with FR-545, 46/6, and G3 A533 exhibiting the highest cross-reactivity capabilities to H7 of A/Shanghai/2/2013. MAb FR-545 was selected for continued assessment, evaluated by IC-IDMS for mAb reactivity against H7 in the H7N9 candidate vaccine virus and compared with/to reactivity to the reference polyclonal antiserum in allantoic fluid, purified whole virus, lyophilized whole virus and final detergent-split monovalent vaccine preparations for vaccine development. IC-IDMS assessment of FR-545 alongside IC-IDMS using the reference polyclonal antiserum to A/Shanghai/2/2013 and with the regulatory SRID method showed strong correlation and mAb IC-IDMS could have played an important role in the event a potential surrogate potency test was required to be rapidly implemented.
http://ift.tt/2uUvcpn
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Τρίτη 1 Αυγούστου 2017
Immunocapture isotope dilution mass spectrometry in response to a pandemic influenza threat
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- SCIAMACHY observed changes in the column mixing ra...
- Multi year aerosol characterization in the tropica...
- Urban air pollution and health risks of parent and...
- Neural Evidence for Non-conscious Working Memory
- Transcription Factors Sp8 and Sp9 Coordinately Reg...
- Efficacy of a novel swallowing exercise program fo...
- Symptom burden and patient characteristics: Associ...
- Efficacy of a novel swallowing exercise program fo...
- Symptom burden and patient characteristics: Associ...
- The Mediator Complex: At the Nexus of RNA Polymera...
- College student with actigraphy performed during f...
- Sleep Phenotypes in Infants and Toddlers with Neur...
- Reduced P300 amplitude during a visuospatial atten...
- Correlation Between the Epworth Sleepiness Scale a...
- Relapse insomnia increases greater risk of anxiety...
- A preliminary examination of gut microbiota, sleep...
- A comparison of the tongue-in-groove and columella...
- CONTENTS 1
- CONTENTS 2
- Cytological and histological changes in the urothe...
- Thyroid Transcription Factor-1 promotes lymph node...
- Exploration of the pathways and interaction networ...
- Evaluation of expression of genes CADM1, TWIST1 an...
- Optical Loupes: A Useful Tool for the Oncall Otorh...
- Optical Loupes: A Useful Tool for the Oncall Otorh...
- Botulinum Toxin Injections Into the Lateral Cricoa...
- Optical Loupes: A Useful Tool for the Oncall Otorh...
- Optical Loupes: A Useful Tool for the Oncall Otorh...
- The impact of onabotulinumtoxinA on severe headach...
- Evaluation of the budding and depth of invasion (B...
- Desensitization to a whole egg by rush oral immuno...
- Cordyceps Sinensis: Anti-fibrotic and inflammatory...
- Biology and management of Avena fatua and Avena lu...
- Is myometrial inflammation a cause or a consequenc...
- Anti-inflammatory effects of exercise training in ...
- Lead-free (Ba,Ca)(Ti,Zr)O3 ceramics within the pol...
- Manufacturing of FeCoCrNiCux medium-entropy alloy ...
- Enhanced strength and ductility of bulk CoCrFeMnNi...
- Duplex stainless steel fabricated by selective las...
- Analyses of a Mutant Foxp3 Allele Reveal BATF as a...
- An antigen-specific semi-therapeutic treatment wit...
- Non-proinflammatory and responsive nanoplatforms f...
- Advance Directives, Hospitalization, and Survival ...
- A Phase II Study with Lead‐In Safety Cohort of 5‐F...
- Comparative efficacy of pharmacological and non-ph...
- Daphnoretin modulates differentiation and maturati...
- Inside front cover
- Naturally occurring immunomodulators with antitumo...
- Characterization and evaluation of nanoencapsulate...
- Reevaluating the Sensory Account of Visual Working...
- Retropharyngeal abscess as a result of hyaluronic ...
- Histopathology of tympanic membranes from patients...
- Tmj total reconstruction with stock and custom mad...
- Vitiligo: Mechanistic Insights Lead to Novel Treat...
- Expansion of cytotoxic CD56bright NK cells during ...
- Expansion of cytotoxic CD56bright NK cells during ...
- Vitiligo: Mechanistic Insights Lead to Novel Treat...
- Retropharyngeal abscess as a result of hyaluronic ...
- Retropharyngeal abscess as a result of hyaluronic ...
- Increasing positive attitudes toward individuals w...
- Increased malignancy incidence in IgE deficient pa...
- Hypogammaglobulinemia E in 216 adults with IgG sub...
- Zika virus infection as a trigger of atopic dermat...
- Increased malignancy incidence in IgE deficient pa...
- Bronchial thermoplasty induces immunomodulation wi...
- Zika virus infection as a trigger of atopic dermat...
- Guidelines for Drinking-Water Quality: Fourth Edit...
- Interventions to Prevent Age-Related Cognitive Dec...
- The black rock series supported SCR catalyst for N...
- PRP Popular for Alopecia Despite Lack of Robust Ev...
- Determinants of Implementation of Isolation Precau...
- Reflector-guided breast tumor localization versus ...
- Screening performance for callosal agenesis in pre...
- Vaginal tumor–vesical fistula detected by dynamic ...
- Pt-decorated zinc oxide nanorod arrays with graphi...
- Perpendicular magnetic anisotropy in ZnMnS ultra-t...
- Anticancer Agents; +345 new citations
- Arthroconidia in Lung Tissue: An Unusual Histopath...
- Bilateral pheochromocytoma with ganglioneuroma com...
- Impedance cardiography in healthy children and chi...
- Executive Functions Impact the Relation Between Re...
- Noise Equally Degrades Central Auditory Processing...
- Carbon dots based photoelectrochemical sensors for...
- Development and application of a novel fluorescent...
- Integrating fluorescent molecularly imprinted poly...
- Mkit: A cell migration assay based on microfluidic...
- The Role of Simulation in Microsurgical Training
- Neurogenic Orofacial Weakness and Speech in Adults...
- In vitro genotoxicity of asbestos substitutes indu...
- Chemical properties of dissolved organic matter de...
- Expression of intelectin-1 in bronchial epithelial...
- Expression of intelectin-1 in bronchial epithelial...
- Bilateral pheochromocytoma with ganglioneuroma com...
- Dendritic cell recruitment and activation in autoi...
- Holding Onto Youth
- Burning Fat and Building Bone by FSH Blockade
- What’s So Special about FGF19—Unique Effects Repor...
- Once Blind, Now We See GLP-1 Molecular Action
- Chronic Sucralose or L-Glucose Ingestion Does Not ...
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