Publication date: July 2018
Source:Biomedicine & Pharmacotherapy, Volume 103
Author(s): Hongtao Wang, Hongli Liu, Shengping Min, Yuanbing Shen, Wei Li, Yuqing Chen, Xiaojing Wang
Cyclin-dependent kinase 16 (CDK16, PCTAIRE1) expression is upregulated in a wide variety of human malignancies. However, the function(s) of CDK16 in non-small cell lung cancer (NSCLC) remain unknown. Therefore, here we investigated the role of CDK16 in NSCLC. From 43 NSCLC tumors and matching healthy control lung tissues, immunohistochemistry revealed significantly greater CDK16 and phospho-p27Ser10 staining levels in NSCLC samples relative to healthy controls. The NSCLC cell line EKVX was transfected with a control siRNA, a CDK16-siRNA, or CDK16-siRNA + p27-siRNA. We found significantly decreased proliferation levels and significantly increased apoptosis levels in CDK16-silenced NSCLC cells. However, these effects were abrogated in cells treated with both the CDK16-siRNA and the p27-siRNA. In CDK16-silenced NSCLC cells, we found upregulated p27 and downregulated phospho-p27Ser10 protein expression but downregulated ubiquitinated p27 and ubiquitinated phospho-p27Ser10 protein expression. Cycloheximide-treated CDK16-silenced NSCLC cells displayed a much milder reduction in p27 protein expression over time relative to untreated CDK16-silenced NSCLC cells. In summary, CDK16 is significantly upregulated in human NSCLC tumor tissue and plays an oncogenic role in NSCLC cells via promoting cell proliferation and inhibiting apoptosis in a p27-dependent manner. Moreover, CDK16 negatively regulates expression of the p27 via ubiquination and protein degradation.
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Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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CDK16 overexpressed in non-small cell lung cancer and regulates cancer cell growth and apoptosis via a p27-dependent mechanism
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- Otolaryngology and the Global Burden of Disease
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- The Small World of Global Otolaryngology
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- Regional Overview of Specific Populations, Workfor...
- Contributors
- Bed II Sequence Stratigraphic context of EF-HR and...
- Technological behaviour in the early Acheulean of ...
- Editorial Board
- Frank Brown (1943–2017)
- Patterns of lateral enamel growth in Homo naledi a...
- Mesopithecus pentelicus from the Turolian locality...
- Reevaluation of ‘endocostal ossifications’ on the ...
- The effect of ontogeny on estimates of KNM-WT 1500...
- A new tephrochronology for early diverse stone too...
- From the Oldowan to the Acheulean at Olduvai Gorge...
- Was the last common ancestor aping a chimp or just...
- Human-like hip joint loading in Australopithecus a...
- A biplanar X-ray approach for studying the 3D dyna...
- New hominin postcranial remains from locality OMO ...
- Large mammal diets and paleoecology across the Old...
- Reconstruction, endocranial form and taxonomic aff...
- Is there a Developed Oldowan A at Olduvai Gorge? A...
- The costs of living at the edge: Seasonal stress i...
- Mandibular ramus shape variation and ontogeny in H...
- Low or Undetectable Basal Thyroglobulin Levels Obv...
- An anti-NMDA receptor encephalitis mimicking an HI...
- Networked Individuals, Gendered Violence: A Litera...
- Editorial Board
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- Retinoid-Sensitive Epigenetic Regulation of the Ho...
- The Dystrophin Glycoprotein Complex Regulates the ...
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- Submucosal Gland Myoepithelial Cells Are Reserve S...
- Editorial Board
- Two cases of discrete adenoid pseudogland formatio...
- Disseminated Kaposi sarcoma with epithelioid morph...
- Oral juvenile xanthogranuloma in a child: Clinical...
- Bone fragility in Turner syndrome: Fracture preval...
- Building Blocks of Social Cognition: Mirror, Menta...
- The role of sentinel lymph node biopsy as a stagin...
- A ticking time bomb: A case of Lyme disease
- A rare case of chronic expanding haematoma in the ...
- Chemotherapy‐induced alopecia: A novel observation
- Noninvasive size reduction of lipoma with an insul...
- The role of sentinel lymph node biopsy as a stagin...
- Contact dermatitis to Dermabond Advance®
- A ticking time bomb: A case of Lyme disease
- Pancreatic panniculitis: A case series from a tert...
- A rare case of chronic expanding haematoma in the ...
- Chemotherapy‐induced alopecia: A novel observation
- Epinephrine, auto-injectors and anaphylaxis: chall...
- Angioedema: systemic activation process during pro...
- Aldolase: a new crustacea allergen
- Table of Contents
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