Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
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Κρήτη 72100
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Τρίτη 19 Φεβρουαρίου 2019

Cross-kingdom interaction of Candida albicans and Actinomyces viscosus elevated cariogenic virulence

Publication date: Available online 18 February 2019

Source: Archives of Oral Biology

Author(s): Ling Deng, Wei Li, Yuanli He, Juan Wu, Biao Ren, Ling Zou

ABSTRACT
Objective

How the interactions betweenCandida albicans and Actinomyces viscosus contributed to the root caries was not clear. This study aimed to investigated their cross-kingdom interactions on the biomass and the cariogenic virulence in dual-species biofilms.

Design

Suspensions ofC. albicans and A.viscosus were formed the mono and polymicrobial biofilms in vitro. Crystal violet assay, viable plate count, scanning electron microscopy and fluorescence in situ hybridization were used to analyze the biomass and biofilm structure. Glycolytic pH drop and the spectrophotometric method were used to evaluate the acid production and hydroxyapatite dissolution, respectively. The exopolysaccharide production was measured by the anthrone-sulfuric acid method, while the adhesion force was measured by atomic force microscopy.

Results

The biomass and colony-forming units of mixed-species was significantly increased compared to that of the mono-speciesat 24 h, 48 h, 72 h.The structure of dual-species biofilm had more microcolonies and was much denser. The dual-species biofilms significantly decreased the pH value and damaged the hydroxyapatite compared with the mono-species biofilms at various time points, indicating the strong cariogenic virulence. Moreover, the dual-species biofilms significantly enhanced the exopolysaccharide production and adhesion force suggesting the increase of biofilm adhesion.

Conclusions

Cross-kingdom interactions ofC. albicans and A. viscosus significantly elevated the biomass and cariogenic virulence of dual-species biofilm.



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