Accumulation of CD5(+)CD19(+) B lymphocytes expressing PD-1 and PD-1L in hypertrophied pharyngeal tonsils.

Related Articles

Accumulation of CD5(+)CD19(+) B lymphocytes expressing PD-1 and PD-1L in hypertrophied pharyngeal tonsils.

Clin Exp Med. 2016 Nov;16(4):503-509

Authors: Wlasiuk P, Niedzielski A, Skorka K, Karczmarczyk A, Zaleska J, Zajac M, Putowski M, Pac-Kozuchowska E, Giannopoulos K

Abstract
Programmed death-1 (PD-1) is one of the most important inhibitory co-receptors expressed predominantly on activated T and B lymphocytes whose expression could be sustained by permanent antigenic stimulation accompanying chronic or recurrent tonsillitis. The expression of PD-1 and PD-1L was analyzed using flow cytometry on hypertrophied tonsils collected from 57 children. We observed high expression of PD-1 and PD-1L on certain lymphocytes subpopulations of hypertrophied tonsils; among T cells, the expression of PD-1 on protein level was higher on CD4(+) cells (70.3 %) than on CD8(+) cells (35 %). Interestingly, a limited expression of PD-1 was observed on CD19(+) B lymphocytes (6.5 %), while CD5(+)CD19(+) B cells overexpressed PD-1 (52.5 %). Moreover, the expression of PD-1L was also higher on CD5(+)CD19(+) B cells (16.5 %) than on CD19(+) B cells (3.5 %) and on CD4(+) T cells (20 %) than on CD8(+) T cells (10 %). PD-1 and PD-1L expressions correlated only on CD5(+)CD19(+) cells. In conclusion, high expression of PD-1 and PD-1L on T and B cells could represent hallmark of immune system adaptation to chronic antigenic exposition in patients with tonsillitis.

PMID: 26319791 [PubMed - indexed for MEDLINE]

http://ift.tt/2kUZDpE