Publication date: Available online 1 July 2017
Source:Vaccine
Author(s): Lixin Wang, Thomas Barclay, Yunmei Song, Paul Joyce, Isaac G. Sakala, Nikolai Petrovsky, Sanjay Garg
Insoluble, nanostructured delta inulin particles enhance the immunogenicity of co-administered protein antigens and consequently are used as a vaccine adjuvant (Advax™). To better understand their immunomodulatory properties, the in vitro hydrolysis and in vivo distribution of delta inulin particles were investigated. Delta inulin particle hydrolysis under bio-relevant acidic conditions resulted in no observable change to the bulk morphology using SEM, and HPLC results showed that only 6.1% of the inulin was hydrolysed over 21days. However, 65% of the terminal glucose groups were released, showing that acid hydrolysis relatively rapidly releases surface bound chemistries. This was used to explain in vivo biodistribution results in which delta inulin particles surface-labelled with fluorescein-5-thiosemicabizide were administered to mice using intramuscular (I.M.) or subcutaneous (S.C.) routes. Comparison analysis of the fluorescence of soluble inulin in the supernatants of homogenised tissues maintained at room temperature or heated to 100°C to solubilise particulate inulin was used to distinguish between fluorescent probe on soluble inulin and probe bound to inulin within particles. Following both I.M. and S.C. injection delta inulin exhibited a depot behaviour with local injection site residence for several weeks. Over this time, as injection site inulin reduced, there was measurable transport of intact delta inulin particles by macrophages to secondary lymphoid organs and the liver. Ultimately, the injected delta inulin became solubilised resulting in its detection in the plasma and in the urine. Thus injected delta inulin particles are initially taken up by macrophages at the site of injection, trafficked to secondary lymphoid tissue and the liver, and hydrolysed resulting in their becoming soluble and diffusing into the blood stream, from whence they are glomerularly filtered and excreted into the urine. These results provide important insights into the biodistribution of I.M. or S.C. injected delta inulin particles when used as vaccine adjuvants and their method of excretion.
http://ift.tt/2sAfjBY
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Ιουλίου
(7890)
-
▼
Ιουλ 02
(134)
- Engineering Dental Implants
- Head and Neck Diseases
- Editorial Board and Contents
- Host Genetics and Gut Microbiome: Challenges and P...
- Re-evaluating Strategies to Define the Immunoregul...
- Molecular Dissection of CD8+ T-Cell Dysfunction
- Consecutive occurrence of isotopic and isomorphic ...
- Superficial plantar angiomyxoma in a young man
- Consecutive occurrence of isotopic and isomorphic ...
- Superficial plantar angiomyxoma in a young man
- The dog days or dog days of summer are the hot, su...
- Brassica rapa hairy root extracts promote skin dep...
- Brassica rapa hairy root extracts promote skin dep...
- Measurement of Pharyngo-laryngeal Volume During Sw...
- Subcutaneous mycoses in Peru: a systematic review ...
- Burden and health-related quality of life of eatin...
- Issue Information
- CIK as therapeutic agents against tumors
- A Case of Atopic Myelitis with Cervical Cavernous ...
- A call to arms of specialty societies to review th...
- The atopic disorders and atopy … “strange diseases...
- Atopic dermatitis treatment: Current state of the ...
- Efficacy and safety of omalizumab in children and ...
- A 6-month safety and efficacy study of fluticasone...
- Asthma in older people hospitalized with influenza...
- Prevalence and triggers of anaphylactic events in ...
- Seasonal variation and monthly patterns of skin sy...
- Association of maternal depression and allergic di...
- A 10-year experience of a novel and safe modified ...
- Pearls and pitfalls: Autoimmune lymphoproliferativ...
- A 28-year-old woman with fever, rash, and pancytop...
- For the Patient: Prevalence and triggers of anaphy...
- Abstracts from the New England Society of Allergy ...
- A call to arms of specialty societies to review th...
- The atopic disorders and atopy … “strange diseases...
- Atopic dermatitis treatment: Current state of the ...
- Efficacy and safety of omalizumab in children and ...
- A 6-month safety and efficacy study of fluticasone...
- Asthma in older people hospitalized with influenza...
- Prevalence and triggers of anaphylactic events in ...
- Seasonal variation and monthly patterns of skin sy...
- Association of maternal depression and allergic di...
- A 10-year experience of a novel and safe modified ...
- Pearls and pitfalls: Autoimmune lymphoproliferativ...
- A 28-year-old woman with fever, rash, and pancytop...
- For the Patient: Prevalence and triggers of anaphy...
- Abstracts from the New England Society of Allergy ...
- Assessment of complete unilateral cleft lip and pa...
- Can Residents Be Trained and Safety Maintained?
- Multiple Non-Syndromic Bilaterally Erupted Para Pr...
- NCI UNC Project ESCC
- Eltrombopag for People With Fanconi Anemia
- NCI UNC Project ESCC
- Eltrombopag for People With Fanconi Anemia
- Contributors
- Contents
- Skin and Composite Grafting Techniques in Facial R...
- Defect of the Eyelids
- Flap Basics III
- Forthcoming Issues
- Flap Basics II
- Index
- Repair of Auricular Defects
- Facial Reconstruction Post-Mohs Surgery
- Reconstruction of Mohs Defects of the Lips and Chin
- Reconstruction of Cheek Defects Secondary to Mohs ...
- Copyright
- In Pursuit of Perfection: The Art of Facial Restor...
- Contributors
- Contents
- Skin and Composite Grafting Techniques in Facial R...
- Defect of the Eyelids
- Flap Basics III
- Forthcoming Issues
- Flap Basics II
- Index
- Repair of Auricular Defects
- Facial Reconstruction Post-Mohs Surgery
- Reconstruction of Mohs Defects of the Lips and Chin
- Reconstruction of Cheek Defects Secondary to Mohs ...
- Copyright
- In Pursuit of Perfection: The Art of Facial Restor...
- Personality predicts the vibrancy of colour imager...
- Emergency Department Flow Measures for Adult and P...
- Autophagy inhibition enabled efficient phototherma...
- Editorial board
- Macrophages with cellular backpacks for targeted d...
- Effects of allergen sensitization on response to t...
- Epinephrine use for anaphylaxis: Too seldom, too late
- Corrigendum to “Management Challenges in a Child w...
- Efficacy of oral immunotherapy with a rice-based e...
- Prodrugs in medicinal chemistry and enzyme prodrug...
- Serum Wisteria floribunda agglutinin-positive Mac-...
- Acceleration of skin wound healing by low-dose ind...
- High frequencies of a favorable IL-28B rs8099917 p...
- Evaluation of Sedation-Related Medication Errors i...
- Synthesis and biological evaluation of novel stero...
- Staged definitive repair after carotid blowout: a ...
- Joint Attention and Oromotor Abilities in Young Ch...
- Geospatial analysis of dissolved nutrients dataset...
-
▼
Ιουλ 02
(134)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Ιουλίου
(7890)
-
▼
Ιουλ 02
(134)
- Engineering Dental Implants
- Head and Neck Diseases
- Editorial Board and Contents
- Host Genetics and Gut Microbiome: Challenges and P...
- Re-evaluating Strategies to Define the Immunoregul...
- Molecular Dissection of CD8+ T-Cell Dysfunction
- Consecutive occurrence of isotopic and isomorphic ...
- Superficial plantar angiomyxoma in a young man
- Consecutive occurrence of isotopic and isomorphic ...
- Superficial plantar angiomyxoma in a young man
- The dog days or dog days of summer are the hot, su...
- Brassica rapa hairy root extracts promote skin dep...
- Brassica rapa hairy root extracts promote skin dep...
- Measurement of Pharyngo-laryngeal Volume During Sw...
- Subcutaneous mycoses in Peru: a systematic review ...
- Burden and health-related quality of life of eatin...
- Issue Information
- CIK as therapeutic agents against tumors
- A Case of Atopic Myelitis with Cervical Cavernous ...
- A call to arms of specialty societies to review th...
- The atopic disorders and atopy … “strange diseases...
- Atopic dermatitis treatment: Current state of the ...
- Efficacy and safety of omalizumab in children and ...
- A 6-month safety and efficacy study of fluticasone...
- Asthma in older people hospitalized with influenza...
- Prevalence and triggers of anaphylactic events in ...
- Seasonal variation and monthly patterns of skin sy...
- Association of maternal depression and allergic di...
- A 10-year experience of a novel and safe modified ...
- Pearls and pitfalls: Autoimmune lymphoproliferativ...
- A 28-year-old woman with fever, rash, and pancytop...
- For the Patient: Prevalence and triggers of anaphy...
- Abstracts from the New England Society of Allergy ...
- A call to arms of specialty societies to review th...
- The atopic disorders and atopy … “strange diseases...
- Atopic dermatitis treatment: Current state of the ...
- Efficacy and safety of omalizumab in children and ...
- A 6-month safety and efficacy study of fluticasone...
- Asthma in older people hospitalized with influenza...
- Prevalence and triggers of anaphylactic events in ...
- Seasonal variation and monthly patterns of skin sy...
- Association of maternal depression and allergic di...
- A 10-year experience of a novel and safe modified ...
- Pearls and pitfalls: Autoimmune lymphoproliferativ...
- A 28-year-old woman with fever, rash, and pancytop...
- For the Patient: Prevalence and triggers of anaphy...
- Abstracts from the New England Society of Allergy ...
- Assessment of complete unilateral cleft lip and pa...
- Can Residents Be Trained and Safety Maintained?
- Multiple Non-Syndromic Bilaterally Erupted Para Pr...
- NCI UNC Project ESCC
- Eltrombopag for People With Fanconi Anemia
- NCI UNC Project ESCC
- Eltrombopag for People With Fanconi Anemia
- Contributors
- Contents
- Skin and Composite Grafting Techniques in Facial R...
- Defect of the Eyelids
- Flap Basics III
- Forthcoming Issues
- Flap Basics II
- Index
- Repair of Auricular Defects
- Facial Reconstruction Post-Mohs Surgery
- Reconstruction of Mohs Defects of the Lips and Chin
- Reconstruction of Cheek Defects Secondary to Mohs ...
- Copyright
- In Pursuit of Perfection: The Art of Facial Restor...
- Contributors
- Contents
- Skin and Composite Grafting Techniques in Facial R...
- Defect of the Eyelids
- Flap Basics III
- Forthcoming Issues
- Flap Basics II
- Index
- Repair of Auricular Defects
- Facial Reconstruction Post-Mohs Surgery
- Reconstruction of Mohs Defects of the Lips and Chin
- Reconstruction of Cheek Defects Secondary to Mohs ...
- Copyright
- In Pursuit of Perfection: The Art of Facial Restor...
- Personality predicts the vibrancy of colour imager...
- Emergency Department Flow Measures for Adult and P...
- Autophagy inhibition enabled efficient phototherma...
- Editorial board
- Macrophages with cellular backpacks for targeted d...
- Effects of allergen sensitization on response to t...
- Epinephrine use for anaphylaxis: Too seldom, too late
- Corrigendum to “Management Challenges in a Child w...
- Efficacy of oral immunotherapy with a rice-based e...
- Prodrugs in medicinal chemistry and enzyme prodrug...
- Serum Wisteria floribunda agglutinin-positive Mac-...
- Acceleration of skin wound healing by low-dose ind...
- High frequencies of a favorable IL-28B rs8099917 p...
- Evaluation of Sedation-Related Medication Errors i...
- Synthesis and biological evaluation of novel stero...
- Staged definitive repair after carotid blowout: a ...
- Joint Attention and Oromotor Abilities in Young Ch...
- Geospatial analysis of dissolved nutrients dataset...
-
▼
Ιουλ 02
(134)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου