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Οκτ 28
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- An Evaluation of Different Bridging Techniques for...
- Use of Fat Grafts in Facial Reconstruction on the ...
- Response to the letter to the editor: “Understandi...
- Microsurgery Fellowship at St Vincent's Hospital, ...
- Significance of the Lateral Thoracic Artery in Pec...
- Gluteal Augmentation With Intramuscular Implants i...
- Reverse Distal Transverse Palmar Arch in Distal Di...
- Does the Sequence of Tattooing and Nipple Reconstr...
- Pediatric Lower Extremity Lawn Mower Injuries and ...
- The Classic Pitanguy Technique and Its Modificatio...
- Identification of Risk Factors in Lymphatic Surger...
- Sweet Syndrome After Bilateral Deep Inferior Epiga...
- The Boreds
- Analysis of Mechanical Behavior of Dermal Fibrobla...
- Free Lateral Digital Flap for Reconstruction of th...
- Acute-Onset Quadriplegia With Recovery After High-...
- The Use of Bilayered Fascia Lata With an Interposi...
- Lesions Associated With Calcium Gluconate Extravas...
- Impact of Abdominal Lipectomy on Post-Sleeve Gastr...
- Chemotherapy Extravasation Management: 21-Year Exp...
- Should you wash your hair everyday?
- Should you wash your hair everyday?
- Editorial Board/Reviewing Committee
- Identification and management of inverted or evert...
- Comparison between chloral hydrate and propofol-ke...
- Carbon nanotubes physicochemical properties influe...
- Editorial Board/Title Page
- The blind mind: No sensory visual imagery in aphan...
- Cover Figure
- Nonfatal air embolism complicating percutaneous CT...
- Mitochondrial specific photodynamic therapy by rar...
- Macrophages as a potential tumor-microenvironment ...
- Dual modal ultra-bright nanodots with aggregation-...
- Porous composite scaffold incorporating osteogenic...
- The treatment of a pregnant HIV positive patient w...
- Sporotrichosis by Sporothrix schenckii senso stric...
- Monogenic lupus: it's all new!
- Chemical affinity of 10-methacryloyloxydecyl dihyd...
- Editorial Board
- Zirconia-incorporated zinc oxide eugenol has impro...
- 3D printed versus conventionally cured provisional...
- Incorporation of antibacterial agent derived deep ...
- Corrosion behavior of titanium in response to sulf...
- Cuspal flexure of composite-restored typodont teet...
- Collagenous matrix supported by a 3D-printed scaff...
- Zirconia surface modification by a novel zirconia ...
- Cytotoxicity and DNA double-strand breaks in human...
- Bioactivity and fluoride release of strontium and ...
- Instrument-assisted soft tissue mobilization incre...
- Standard classification and pathophysiology of ros...
- Sleep disturbance in children with moderate/severe...
- Evidence-based update on rosacea comorbidities and...
- Quantitative analysis of facial soft tissue perfus...
- Treatment of lower lip pits in Van der Woude syndr...
- Hyperlipidemia-induced hepassocin in the liver con...
- Activin over-expression in the testis of mice lack...
- Cerebellar ataxia with neuropathy and vestibular a...
- A rare case of bilateral vagus nerve schwanomatosis
- Cerebellar ataxia with neuropathy and vestibular a...
- A rare case of bilateral vagus nerve schwanomatosis
- Morphology and Outcomes of Total Endovascular Trea...
- Determinants of Acute Kidney Injury and Renal Func...
- Modern Hearing Aids: Verification, Outcome Measure...
- Improving the Efficiency of Speech-In-Noise Hearin...
- Hearing Threshold Shifts Among 11-to-35-Year-Olds ...
- Rotational and Collic Vestibular-Evoked Myogenic P...
- Systemic Aminoglycosides-Induced Vestibulotoxicity...
- Comparison of the Spectral-Temporally Modulated Ri...
- Rehabilitation and Psychosocial Determinants of Co...
- Age-Related Differences in the Processing of Tempo...
- The Effect of Aging and Priming on Same/Different ...
- Children With Single-Sided Deafness Use Their Coch...
- The Advances in Hearing Rehabilitation and Cochlea...
- Congenital Deafness Alters Sensory Weighting for P...
- Speech Detection in Noise for Young Bilaterally Im...
- Vibrotactile Thresholds on the Mastoid and Forehea...
- Auditory Brainstem Implant Array Position Varies W...
- Noise History and Auditory Function in Young Adult...
- Cantonese Tone Perception for Children Who Use a H...
- A custom-made mouthpiece incorporating tongue depr...
- Inflammation is regulated by the adenosine derivat...
- Dengue hemorrhagic fever complicated with transien...
- Dengue hemorrhagic fever complicated with transien...
- Disability in activities of daily living among adu...
- The clinical development of vaccines for HER2+ bre...
- Editorial Board
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- Editorial Board
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! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Σάββατο 28 Οκτωβρίου 2017
An Evaluation of Different Bridging Techniques for Short Nerve Gaps
http://ift.tt/2id60Ve
Use of Fat Grafts in Facial Reconstruction on the Wounded Soldiers From the First World War (WWI) by Hippolyte Morestin (1869–1919)
http://ift.tt/2id60oc
Response to the letter to the editor: “Understanding the impact of preservation methods on the integrity and functionality of placental allografts”
Significance of the Lateral Thoracic Artery in Pectoralis Major Musculocutaneous Flap Reconstruction: Quantitative Assessment of Blood Circulation Using Indocyanine Green Angiography
http://ift.tt/2gLqrsd
Gluteal Augmentation With Intramuscular Implants in Patients With Human Immunodeficiency Virus With Lipoatrophy Related to the Use of Antiretroviral Therapy
http://ift.tt/2gKGt5y
Reverse Distal Transverse Palmar Arch in Distal Digital Replantation
http://ift.tt/2gKELRA
Does the Sequence of Tattooing and Nipple Reconstruction Affect Nipple Projection?
http://ift.tt/2gMBVvf
Pediatric Lower Extremity Lawn Mower Injuries and Reconstruction: Retrospective 10-Year Review at a Level 1 Trauma Center
http://ift.tt/2gLNIdm
The Classic Pitanguy Technique and Its Modifications in Mammaplasty: Ten Years of Experiences
http://ift.tt/2gLqqo9
Identification of Risk Factors in Lymphatic Surgeries for Melanoma: A National Surgical Quality Improvement Program Review
http://ift.tt/2gMoxrl
Sweet Syndrome After Bilateral Deep Inferior Epigastric Perforator Flap Breast Reconstruction: A Case Report
http://ift.tt/2gKL4og
Analysis of Mechanical Behavior of Dermal Fibroblasts Obtained From Various Anatomical Sites in Humans
http://ift.tt/2id5LJO
Free Lateral Digital Flap for Reconstruction of the Fingers
http://ift.tt/2idNDjd
Acute-Onset Quadriplegia With Recovery After High-Voltage Electrical Injury
http://ift.tt/2icy3nY
The Use of Bilayered Fascia Lata With an Interpositional Omental Flap for Autologous Repair of Contaminated Abdominal Fascial Defects
http://ift.tt/2id5FSs
Lesions Associated With Calcium Gluconate Extravasation: Presentation of 5 Clinical Cases and Analysis of Cases Published
http://ift.tt/2idi3BN
Impact of Abdominal Lipectomy on Post-Sleeve Gastrectomy Surgery Weight Loss
http://ift.tt/2icBNFM
Chemotherapy Extravasation Management: 21-Year Experience
http://ift.tt/2id5CGg
Should you wash your hair everyday?
For some people, frequent washing can damage hair and cause a dry, itchy scalp. How often should you wash your hair with shampoo?
http://ift.tt/2gS26oh
Should you wash your hair everyday?
For some people, frequent washing can damage hair and cause a dry, itchy scalp. How often should you wash your hair with shampoo?
http://ift.tt/2gS26oh
Identification and management of inverted or everted edges of traumatic tympanic membrane perforations
Publication date: Available online 28 October 2017
Source:Brazilian Journal of Otorhinolaryngology
Author(s): Zhengcai Lou, Zi-Han Lou
IntroductionMost of traumatic tympanic membrane perforations have inverted or everted edges, however, the effects of inverted and everted edges on the spontaneous healing of the eardrum remain controversial.ObjectiveWe investigated the influence of inverted or everted edges on the spontaneous healing of traumatic tympanic membrane perforations.MethodsThe clinical records of patients with a traumatic tympanic membrane perforations who met the study criteria were retrieved and categorized into two groups, based on whether the eardrum was inverted or everted. The features along the edge of each inverted or everted eardrum were described using 30° and 70° endoscopes.ResultsIn total, 196 patients (196 ears) met the inclusion criteria; of these, 148 had inverted or everted eardrums while 48 did not. Of the 148 patients with inverted or everted eardrums, the perforation edges were everted in 77 patients, inverted in 44 patients, drooping in 17 patients, and both inverted and everted in 10 patients. The perforation shape was triangular in 18.9% of patients, sector-shaped in 11.5%, kidney-shaped in 14.2%, ovoid in 20.3%, and irregularly shaped in 35.1% of patients. The difference was not significant between the with and without inverted/everted eardrum edges groups in terms of the closure rate or closure time. Similarly, the difference was not significant between the with and without edge approximation groups in terms of the closure rate or closure time at the end of the 12-month follow-up period.ConclusionThis study suggests that endoscopic inspection can clearly identify inverted/everted eardrum edges using 30° and 70° endoscopes. The edge is glossy in inverted/everted eardrums, whereas the edge is rough and irregular in non-inverted/everted cases. The inverted/everted eardrums gradually became necrotic, but this did not affect the healing process. Additionally, edge approximation did not improve the healing outcome of traumatic tympanic membrane perforations.
http://ift.tt/2icjlNI
Comparison between chloral hydrate and propofol-ketamine as sedation regimens for pediatric auditory brainstem response testing
Publication date: Available online 28 October 2017
Source:Brazilian Journal of Otorhinolaryngology
Author(s): Kamal Abulebda, Vinit J. Patel, Sheikh S. Ahmed, Alvaro J. Tori, Riad Lutfi, Samer Abu-Sultaneh
IntroductionThe use of diagnostic auditory brainstem response testing under sedation is currently the "gold standard" in infants and young children who are not developmentally capable of completing the test.ObjectiveThe aim of the study is to compare a propofol-ketamine regimen to an oral chloral hydrate regimen for sedating children undergoing auditory brainstem response testing.MethodsPatients between 4 months and 6 years who required sedation for auditory brainstem response testing were included in this retrospective study. Drugs doses, adverse effects, sedation times, and the effectiveness of the sedative regimens were reviewed.Results73 patients underwent oral chloral hydrate sedation, while 117 received propofol-ketamine sedation. 12% of the patients in the chloral hydrate group failed to achieve desired sedation level. The average procedure, recovery and total nursing times were significantly lower in the propofol-ketamine group. Propofol-ketamine group experienced higher incidence of transient hypoxemia.ConclusionBoth sedation regimens can be successfully used for sedating children undergoing auditory brainstem response testing. While deep sedation using propofol-ketamine regimen offers more efficiency than moderate sedation using chloral hydrate, it does carry a higher incidence of transient hypoxemia, which warrants the use of a highly skilled team trained in pediatric cardio-respiratory monitoring and airway management.
http://ift.tt/2gKCNka
Carbon nanotubes physicochemical properties influence the overall cellular behavior and fate
Publication date: Available online 28 October 2017
Source:NanoImpact
Author(s): Reem Eldawud, Alixandra Wagner, Chenbo Dong, Todd Stueckle, Yon Rojanasakul, Cerasela Zoica Dinu
The unique properties of single walled carbon nanotubes (SWCNTs) make them viable candidates for versatile implementation in the next generation of biomedical devices for targeted delivery of chemotherapeutic agents or cellular-sensing probes. Such implementation requires user-tailored changes in SWCNT's physicochemical characteristics to allow for efficient cellular integration while maintaining nanotubes' functionality. However, isolated reports showed that user-tailoring could induce deleterious effects in exposed cells, from decrease in cellular proliferation, to changes in cellular adhesion, generation of reactive oxygen species or phenotypical variations, just to name a few. Before full implementation of SWCNTs is achieved, their toxicological profiles need to be mechanistically correlated with their physicochemical properties to determine how the induced cellular fate is related to the exposure conditions or samples' characteristics. Our study provides a comprehensive analysis of the synergistic cyto- and genotoxic effects resulted from short-term exposure of human lung epithelial cells to pristine (as manufactured) and user-tailored SWCNTs, as a function of their physicochemical properties. Specifically, through a systematic approach we are correlating the nanotube uptake and nanotube-induced cellular changes to the sample's physicochemical characteristics (e.g., metal impurities, length, agglomerate size, surface area, dispersion, and surface functionalization). By identifying changes in active hallmarks involved in cell-cell connections and maintaining epithelial layer integrity, we also determine the role that short-term exposure to SWCNTs plays in the overall cellular fate and cellular transformation. Lastly, we assess cellular structure-function relationships to identify non-apoptotic pathways induced by SWCNTs exposure that could however lead to changes in cellular behavior and cellular transformation. Our results show that the degree of cell transformation is a function of the physicochemical properties of the SWCNT, with the nanotube with higher length, higher metal content and larger agglomerate size reducing cell viability to a larger extent. Such changes in cell viability are also complemented by changes in cell structure, cycle and cell-cell interactions, all responsible for maintaining cell fate.
Graphical abstract
http://ift.tt/2iGi45l
Editorial Board/Title Page
Publication date: November 2017
Source:Cortex, Volume 96
http://ift.tt/2iHFU0v
The blind mind: No sensory visual imagery in aphantasia
Source:Cortex
Author(s): Rebecca Keogh, Joel Pearson
For most people the use of visual imagery is pervasive in daily life, but for a small group of people the experience of visual imagery is entirely unknown. Research based on subjective phenomenology indicates that otherwise healthy people can completely lack the experience of visual imagery, a condition now referred to as aphantasia. As congenital aphantasia has thus far been based on subjective reports, it remains unclear whether participants are really unable to imagine visually, or if they have very poor metacognition - they have images in their mind, but are blind to them. Here we measured sensory imagery in subjectively self-diagnosed aphantasics, using the binocular rivalry paradigm, as well as measuring their self-rated object and spatial imagery with multiple questionnaires (VVIQ, SUIS and OSIQ). Unlike, the general population, experimentally naive aphantasics showed almost no imagery-based rivalry priming. Aphantasic participants' self-rated visual object imagery was significantly below average, however their spatial imagery scores were above average. These data suggest that aphantasia is a condition involving a lack of sensory and phenomenal imagery, and not a lack of metacognition. The possible underlying neurological cause of aphantasia are discussed as well as future research directions.
http://ift.tt/2zg31qf
Cover Figure
Source:Cortex, Volume 96
http://ift.tt/2iJCWIR
Nonfatal air embolism complicating percutaneous CT-guided lung biopsy and VATS marking: Four cases from a single institution
Publication date: March–April 2018
Source:Clinical Imaging, Volume 48
Author(s): Rika Yoshida, Takeshi Yoshizako, Megumi Nakamura, Shinji Ando, Mitsunari Maruyama, Minako Maruyama, Yoshikazu Takinami, Yukihisa Tamaki, Tomonori Nakamura, Hajime Kitagaki
Systemic air emboli occur as a rare complication of percutaneous needle biopsy of the lung and video-assisted thoracoscopic surgery (VATS) marking.Here we present four cases of systemic air emboli from single institution and the imaging findings and embolism' kinetics using contrast-enhanced media during VATS color marking with indocyanine green. We suggest that early detection using routine whole-lung CT is required for asymptomatic patients with abnormal air. If abnormal air is found, we should keep the patient to the appropriate posture in order to prevent moving the air until it dissipates. Early detection of abnormal air can prevent severe complications.
http://ift.tt/2iHbJXj
Mitochondrial specific photodynamic therapy by rare-earth nanoparticles mediated near-infrared graphene quantum dots
Source:Biomaterials, Volume 153
Author(s): Dandan Zhang, Liewei Wen, Ru Huang, Huanhuan Wang, Xianglong Hu, Da Xing
Photodynamic therapy (PDT) has been proposed in cancer treatment for decades, but its clinical translation is significantly impeded by the low yield of ROS, poor tissue penetration depth of most current photosensitizers, and short lifetime of ROS. These limitations directly affect the therapeutic effect of PDT in cancer therapy. Here we proposed a new strategy by collaboratively integrating rare-earth doped upconversion nanoparticles (UCNP) with graphene quantum dot (GQD) for highly efficacious PDT, based on the merits of UCNP, which can emit UV–vis light under near-infrared light (NIR) excitation, and GQD, which can produce 1O2 efficiently. For GQD-decorated UCNP nanoparticles (UCNP-GQD), the emission light from UCNP can further excite GQD with prominent 1O2 generation for NIR-triggered PDT. Furthermore, a hydrophilic rhodamine derivative, TRITC, is covalently tethered to afford the resultant UCNP-GQD/TRITC, possessing distinct mitochondrial targeting property. Thus mitochondrial specific PDT with in-situ1O2 burst in mitochondria induces sharp decrease of mitochondrial membrane potential, which initiates the tumor cell apoptosis irreversibly. Importantly, in vivo experiments demonstrate the tumor inhibition of mitochondrial targeting UCNP-GQD/TRITC with improved therapeutic efficiency compared with non-targeting UCNP-GQD. The proposed strategy highlights the advantages of precision organelles-specific PDT in cancer therapy.
http://ift.tt/2lqk5n6
Macrophages as a potential tumor-microenvironment target for noninvasive imaging of early response to anticancer therapy
Source:Biomaterials, Volume 152
Author(s): Qizhen Cao, Xinrui Yan, Kai Chen, Qian Huang, Marites P. Melancon, Gabriel Lopez, Zhen Cheng, Chun Li
As a result of therapy-induced apoptosis, peripheral blood monocytes are recruited to tumors, where they become tumor-associated macrophages (TAMs). To date, few studies have investigated noninvasive molecular imaging for assessment of macrophage infiltration in response to therapy-induced apoptosis. Here, noninvasive assessment of changes in tumor accumulation of TAMs was proposed as a new way to measure early tumor response to anticancer therapy. Three different nanoparticles, QD710-Dendron quantum dots (QD710-D), Ferumoxytol, and PG-Gd-NIR813, were used for near-infrared fluorescence imaging, T2-weighted magnetic resonance imaging, and dual optical/T1-weighted MR imaging, respectively, in the MDA-MB-435 tumor model. Treatment with Abraxane induced tumor apoptosis and infiltrating macrophages. In spite of markedly different physicochemical properties among the nanoparticles, in vivo imaging revealed increased uptake of all three nanoparticles in Abraxane-treated tumors compared with untreated tumors. Moreover, imaging visualized increased uptake of QD710-D in MDA-MB-435 tumors but not in drug-resistant MDA-MB-435R tumors grown in the mice treated with Abraxane. Our results suggest that infiltration of macrophages due to chemotherapy-induced apoptosis was partially responsible for increased nanoparticle uptake in treated tumors. Noninvasive imaging techniques in conjunction with systemic administration of imageable nanoparticles that are taken up by macrophages are a potentially useful tool for assessing early treatment response.
http://ift.tt/2lpmEWA
Dual modal ultra-bright nanodots with aggregation-induced emission and gadolinium-chelation for vascular integrity and leakage detection
Source:Biomaterials, Volume 152
Author(s): Guangxue Feng, Jackson Liang Yao Li, Carla Claser, Akhila Balachander, Yingrou Tan, Chi Ching Goh, Immanuel Weng Han Kwok, Laurent Rénia, Ben Zhong Tang, Lai Guan Ng, Bin Liu
The study of blood brain barrier (BBB) functions is important for neurological disorder research. However, the lack of suitable tools and methods has hampered the progress of this field. Herein, we present a hybrid nanodot strategy, termed AIE-Gd dots, comprising of a fluorogen with aggregation-induced emission (AIE) characteristics as the core to provide bright and stable fluorescence for optical imaging, and gadolinium (Gd) for accurate quantification of vascular leakage via inductively-coupled plasma mass spectrometry (ICP-MS). In this report, we demonstrate that AIE-Gd dots enable direct visualization of brain vascular networks under resting condition, and that they form localized punctate aggregates and accumulate in the brain tissue during experimental cerebral malaria, indicative of hemorrhage and BBB malfunction. With its superior detection sensitivity and multimodality, we hereby propose that AIE-Gd dots can serve as a better alternative to Evans blue for visualization and quantification of changes in brain barrier functions.
http://ift.tt/2ydih6M
Porous composite scaffold incorporating osteogenic phytomolecule icariin for promoting skeletal regeneration in challenging osteonecrotic bone in rabbits
Publication date: January 2018
Source:Biomaterials, Volume 153
Author(s): Yuxiao Lai, Huijuan Cao, Xinluan Wang, Shukui Chen, Ming Zhang, Nan Wang, Zhihong Yao, Yi Dai, Xinhui Xie, Peng Zhang, Xinsheng Yao, Ling Qin
Steroid-associated osteonecrosis (SAON) often requires surgical core decompression (CD) in the early stage for removal of necrotic bone to facilitate repair where bone grafts are needed for filling bone defect and avoiding subsequent joint collapse. In this study, we developed a bioactive composite scaffold incorporated with icariin, a unique phytomolecule that can provide structural and mechanical support and facilitate bone regeneration to fill into bone defects after surgical CD in established SAON rabbit model. An innovative low-temperature 3D printing technology was used to fabricate the poly (lactic-co-glycolic acid)/β-calcium phosphate/icariin (PLGA/TCP/Icariin, PTI) scaffold. The cytocompatibility of the PTI scaffold was tested in vitro, and the osteogenesis properties of PTI scaffolds were assessed in vivo in the SAON rabbit models. Our results showed that the fabricated PTI scaffold had a well-designed biomimic structure that was precisely printed to provide increased mechanical support and stable icariin release from the scaffold for bone regeneration. Furthermore, our in vivo study indicated that the PTI scaffold could enhanced the mechanical properties of new bone tissues and improved angiogenesis within the implanted region in SAON rabbit model than those of PLGA/TCP (PT) scaffold. The underlying osteoblastic mechanism was investigated using MC3T3-E1 cells in vitro and revealed that icariin could facilitate MC3T3-E1 cells ingrowth into the PTI scaffold and regulate osteoblastic differentiation. The PTI scaffold exhibited superior biodegradability, biocompatibility, and osteogenic capability compared with those of PT scaffold. In summary, the PTI composite scaffold which incorporated bioactive phyto-compounds is a promising potential strategy for bone tissue engineering and regeneration in patients with challenging SAON.
Graphical abstract
http://ift.tt/2lqVbE6
The treatment of a pregnant HIV positive patient with cryptococcal meningitis in Malawi. Case report and review of treatment options
Publication date: Available online 28 October 2017
Source:Medical Mycology Case Reports
Author(s): Philip D. Bright, Duncan Lupiya, Joep J. van Oosterhout, Amy Chen, Thomas S. Harrison, Adrienne K. Chan
This case reports cryptococcal meningitis in an HIV positive woman on antiretroviral therapy, presenting with left middle cerebral artery stroke at 30 weeks gestation. The patient had well-controlled HIV (CD4 count over 200 cells/mL). The immunosuppressive effects of the pregnancy likely contributed to the development of cryptococcal disease. The patient was successfully treated with two weeks of amphotericin B followed by fluconazole, delivered a healthy baby, but remained with a permanent severe neurological deficit.
http://ift.tt/2xwcMMi
Sporotrichosis by Sporothrix schenckii senso stricto with itraconazole resistance and terbinafine sensitivity observed in vitro and in vivo: case report
Publication date: Available online 28 October 2017
Source:Medical Mycology Case Reports
Author(s): Rodrigo Vettorato, Daiane Heidrich, Fernanda Fraga, Amanda Carvalho Ribeiro, Danielle Machado Pagani, Carina Timotheo, Tais Guarienti Amaro, Gerson Vettorato, Maria Lúcia Scroferneker
We report a case of a patient with lymphocutaneous sporotrichosis in the right upper limb. The fungus was identified as Sporothrix schenckii senso stricto by calmodulin gene sequencing. The initial treatment was itraconazole (200mg/day), but in vitro antifungal susceptibility demonstrated high resistant to this and another six antifungals, with exception to terbinafine. The lesions did not regress with itraconazole treatment. Thus, 500mg/day of terbinafine was prescribed and clinical cure was obtained after four months
http://ift.tt/2zXCsCY
Monogenic lupus: it's all new!
Patricia Costa-Reis | Kathleen E Sullivan
http://ift.tt/2hkjHST
Chemical affinity of 10-methacryloyloxydecyl dihydrogen phosphate to dental zirconia: Effects of molecular structure and solvents
Publication date: Available online 14 October 2017
Source:Dental Materials
Author(s): Ying Chen, Zhicen Lu, Mengke Qian, Huaiqin Zhang, Chen Chen, Haifeng Xie, Franklin R. Tay
ObjectivesTo examine whether solvents and changing the molecular structure of 10-Methacryloyloxydecyl dihydrogen phosphate (10-MDP) affect its chemical affinity to Yttria-stabilized tetragonal zirconia polycrystals (Y-TZP).MethodsThe present work investigated the chemical affinity between Y-TZP and 10-MDP dissolved in different solvents (acetone/ethanol/water or mixture) using X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, and thermodynamic calculations. Shear bond strength (SBS) tests were used to evaluate the influence of different solvents on 10-MDP bonding. In addition, several phosphate ester monomer variants were created by changing the 10-MDP molecular structure. Changes included extending/shortening the spacer chain-length, and installing hydroxyl or carboxyl groups as side chains at different positions along the spacer chain. The thermodynamic parameters of the complexes formed between the 10-MDP variants and tetragonal zirconia were evaluated.ResultsThe acquired data indicated that solvent is necessary for the formation of Zr–O–P bonds between 10-MDP and Y-TZP. Solvents affected the chemical affinity of 10-MDP to Y-TZP; acetone facilitated the best bonding, followed by ethanol. Changing the molecular structure of 10-MDP affected its chemical affinity to Y-TZP. The variants 15-MPDP, 12-MDDP, 6-hydroxyl-10-MDP and 6-carboxy-10-MDP all exhibited higher thermodynamic stability than 10-MDP when coordinated with tetragonal zirconia. In contrast, 2-MEP, 5-MPP, 10-hydroxyl-MDP, 10-carboxy-MDP, 5,6-dihydroxyl-10-MDP and 5,6-dicarboxy-10-MDP exhibited lower thermodynamic stability.Significance10-MDP coordinates with zirconia through dissociating in solvents. Changing the molecular structure of 10-MDP theoretically affects its chemical affinity to Y-TZP.
Graphical abstract
http://ift.tt/2zgM12M
Editorial Board
Source:Dental Materials, Volume 33, Issue 11
http://ift.tt/2zfo4Jm
Zirconia-incorporated zinc oxide eugenol has improved mechanical properties and cytocompatibility with human dental pulp stem cells
Publication date: Available online 14 October 2017
Source:Dental Materials
Author(s): Soo Kyung Jun, Hae-Won Kim, Hae-Hyoung Lee, Jung-Hwan Lee
ObjectiveZinc oxide eugenol (ZOE) is widely used as a therapeutic dental restorative material. However, ZOE has poor mechanical properties and high cytotoxicity toward human dental pulp stem cells (hDPSCs) due to the release of Zn ions. In this study, zirconia-incorporated ZOE (ZZrOE) was developed to reduce the cytotoxicity and improve the mechanical properties of ZOE with sustained therapeutic effects on inflamed hDPSCs in terms of inflammatory gene expression levels compared with those of the original material.MethodsAfter the setting time and mechanical properties of ZZrOE incorporating varying amounts of zirconia (0, 5, 10, and 20wt% in powder) were characterized, the surface morphology and composition of the resulting ZZrOE materials were investigated. The ions and chemicals released into the cell culture medium from ZOE and ZZrOE (3cm2/mL) were measured by inductively coupled plasma atomic emission spectroscopy and gas chromatography, respectively. After testing cytotoxicity against hDPSCs using the above extracts, the therapeutic effects on lipopolysaccharide-inflamed hDPSCs in terms of compromising the upregulation of inflammatory response-related mRNA expression were tested using real-time PCR.ResultsZZrOE 20% exhibited increased compressive strength (∼45%), 3-point flexural strength (∼150%) and hardness (∼75%), as well as a similar setting time (∼90%), compared with those of ZOE. After the rough surface of ZZrOE was observed, significantly fewer released Zn ions and eugenol (∼40% of that from ZOE) were detected in ZZrOE 20%. ZZrOE showed less cytotoxicity because of the lower amount of Zn ions released from ZOE while showing sustained inhibition of inflammatory marker (e.g., interleukin 1β, 6 and 8) mRNA levels.SignificanceThe improved mechanical properties and cytocompatibility, as well as the sustained therapeutic effects on inflamed hDPSCs, were investigated in ZZrOE compared with those of ZOE. Therefore, ZZrOE has the potential to be used as an alternative to ZOE as a dental restorative material.
Graphical abstract
http://ift.tt/2iGbb3R
3D printed versus conventionally cured provisional crown and bridge dental materials
Source:Dental Materials
Author(s): Anthony Tahayeri, MaryCatherine Morgan, Ana P. Fugolin, Despoina Bompolaki, Avathamsa Athirasala, Carmem S. Pfeifer, Jack L. Ferracane, Luiz E. Bertassoni
ObjectivesTo optimize the 3D printing of a dental material for provisional crown and bridge restorations using a low-cost stereolithography 3D printer; and compare its mechanical properties against conventionally cured provisional dental materials.MethodsSamples were 3D printed (25×2×2mm) using a commercial printable resin (NextDent C&B Vertex Dental) in a FormLabs1+ stereolithography 3D printer. The printing accuracy of printed bars was determined by comparing the width, length and thickness of samples for different printer settings (printing orientation and resin color) versus the set dimensions of CAD designs. The degree of conversion of the resin was measured with FTIR, and both the elastic modulus and peak stress of 3D printed bars was determined using a 3-point being test for different printing layer thicknesses. The results were compared to those for two conventionally cured provisional materials (Integrity®, Dentsply; and Jet®, Lang Dental Inc.).ResultsSamples printed at 90° orientation and in a white resin color setting was chosen as the most optimal combination of printing parameters, due to the comparatively higher printing accuracy (up to 22% error), reproducibility and material usage. There was no direct correlation between printing layer thickness and elastic modulus or peak stress. 3D printed samples had comparable modulus to Jet®, but significantly lower than Integrity®. Peak stress for 3D printed samples was comparable to Integrity®, and significantly higher than Jet®. The degree of conversion of 3D printed samples also appeared higher than that of Integrity® or Jet®.SignificanceOur results suggest that a 3D printable provisional restorative material allows for sufficient mechanical properties for intraoral use, despite the limited 3D printing accuracy of the printing system of choice.
http://ift.tt/2iHDaQC
Incorporation of antibacterial agent derived deep eutectic solvent into an active dental composite
Publication date: Available online 23 October 2017
Source:Dental Materials
Author(s): Jing Wang, Xiaoqing Dong, Qingsong Yu, Sheila N. Baker, Hao Li, Nathaniel E. Larm, Gary A. Baker, Liang Chen, Jingwen Tan, Meng Chen
ObjectiveTo incorporate an antibacterial agent derived deep eutectic solvent (DES) into a dental resin composite, and investigate the resulting mechanical properties and antibacterial effects.MethodThe DES was derived from benzalkonium chloride (BC) and acrylic acid (AA) and was incorporated into the dental resin composite through rapid mixing. A three-point bending test was employed to measure the flexural strength of the composite. An agar diffusion test was used to investigate antibacterial activity. Artificial (accelerated) aging was undertaken by immersing the composites in buffer solutions at an elevated temperature for up to 4 weeks. UV–vis spectrophotometry and NMR analysis were conducted to study BC release from the composite. Finally, the biocompatibility of the composite materials was evaluated using osteoblast cell culture for 7 days. Results were compared to those of a control composite which contained no BC.ResultThe DES-incorporated composite (DES-C) displayed higher flexural strength than a similar BC-incorporated composite BC (BC-C) for the same level of BC. The inclusion of BC conferred antibacterial activity to both BC-containing composites, although BC-C produced larger inhibition halos than DES-C at the same loading of BC. Control composites which contained no BC showed negligible antibacterial activity. After artificial aging, the DES-C composite showed better maintenance of the mechanical properties of the control compared with BC-C, although a decrease was observed during the three-point bending test, particularly upon storage at elevated temperatures. No BC release was detected in the aged solutions of DES-C, whereas the BC-C showed a linear increase in BC release with storage time. Significantly, cell viability results indicated that DES-C has better biocompatibility than BC-C.SignificanceThe incorporation of a BC-based DES into a dental resin composite provides a new strategy to develop antibacterial dental materials with better biocompatibility and longer effective lifetimes without sacrificing the intrinsic mechanical properties of the composite structure.
Graphical abstract
http://ift.tt/2iJsIYZ
Corrosion behavior of titanium in response to sulfides produced by Porphyromonas gingivalis
Publication date: Available online 20 October 2017
Source:Dental Materials
Author(s): Rino Harada, Eitoyo Kokubu, Hideaki Kinoshita, Masao Yoshinari, Kazuyuki Ishihara, Eiji Kawada, Shinji Takemoto
ObjectiveTo investigate the effects of sulfides produced by Porphyromonas gingivalis (P. gingivalis) on the corrosion behavior of titanium.MethodsCommercially pure titanium disks were mirror-polished and immersed in culture medium (BHI), spent medium after culturing P. gingivalis (BHI-S), and culture medium with P. gingivalis (BHI-P), and incubated aerobically at 37°C for 3–14 days. Titanium corrosion was evaluated through surface observation (using scanning electron microscope: SEM), color change (ΔE*ab), glossiness (Gs(20°)), chemical composition and state (using X-ray photoelectron spectroscopy: XPS), and titanium release.ResultsΔE*ab and Gs(20°) did not significantly differ among specimens placed in test mediums for the study duration (p>0.05). SEM images of specimens showed no signs of localized or overall corrosion. XPS analysis indicated showed clear titanium metal state peaks on all specimens in addition to sulfide and sulfate on BHI-S and BHI-P specimens. Valency fraction of titanium decomposed from Ti2p spectrum of BHI-S and BHI-P specimens indicated no progression of oxidation. No significant levels of titanium release were found regardless of the mediums' sulfide content. Results suggested that sulfides produced by P. gingivalis attached on the surface of titanium specimens but did not cause titanium corrosion over the immersion period of 14 days.SignificanceIt is imperative for dental practitioners to be aware of any elements which may influence the clinical success of titanium implants.
Graphical abstract
http://ift.tt/2iINx73
Cuspal flexure of composite-restored typodont teeth and correlation with polymerization shrinkage values
Source:Dental Materials
Author(s): Taylor Enochs, Anne E. Hill, Cassandra E. Worley, Crisnicaw Veríssimo, Daranee Tantbirojn, Antheunis Versluis
ObjectiveThe relationship between post-gel shrinkage, total shrinkage, and cuspal flexure was examined. Cuspal flexure was measured on restored typodont teeth, which offered a standardized tooth shape for comparison of shrinkage stress effects among restorative composites.MethodsSix restorative composites were compared (Filtek LS, Venus Flowable, Tetric EvoCeram, Filtek Flowable, Esthet-X, and Filtek Supreme). Total shrinkage was determined from changes in projected surface area before and after polymerization (n=10). Post-gel shrinkage was determined with a biaxial strain gauge that measured strain development during polymerization (n=10). Cuspal flexure was determined using typodont maxillary second premolars with standard MOD slot preparation (n=10). Flexure was determined by comparing the three-dimensionally scanned cuspal surfaces before and after restoration. Restoration bonding to the typodont cavity was achieved by sandblasting and adhesive application. Bond integrity was verified by measuring dye penetration. Results were analyzed using ANOVA and Student–Newman–Keuls post hoc test (significance level 0.05). Pearson was used for correlations.ResultsTotal and post-gel shrinkage were significant different for all composites (t-test; P<0.001). Depending on the composite, only 9–41% of the total shrinkage was recorded as post-gel shrinkage. Bond integrity of restored typodont teeth was 96–99%. Cuspal flexure correlated strongly with post-gel shrinkage, but there was no correlation with total shrinkage.SignificanceCuspal flexure of restored typodont teeth showed the effect of shrinkage stress caused by polymerizing composite restorations, ensuring standardization while maintaining the effects of tooth/cavity geometry. Post-gel shrinkage gave a good indication to screen composites for the stress they may generate; total shrinkage had no direct correlation with stress.
http://ift.tt/2iIwLF0
Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells
Publication date: Available online 18 October 2017
Source:Dental Materials
Author(s): Farahnaz Fahimipour, Erfan Dashtimoghadam, Morteza Rasoulianboroujeni, Mostafa Yazdimamaghani, Kimia Khoshroo, Mohammadreza Tahriri, Amir Yadegari, Jose A. Gonzalez, Daryoosh Vashaee, Douglas C. Lobner, Tahereh S. Jafarzadeh Kashi, Lobat Tayebi
ObjectiveA systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs).MethodsThe scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures, which optimized for fabrication of 3D printed scaffolds with interconnected porosity. The hybrid constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing.ResultsThe in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the Collagen matrix improves the ALP activity.SignificanceThe presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.
Graphical abstract
http://ift.tt/2zeLRcf
Zirconia surface modification by a novel zirconia bonding system and its adhesion mechanism
Source:Dental Materials
Author(s): Takahiro Murakami, Shinji Takemoto, Norihiro Nishiyama, Masahiro Aida
ObjectiveBonding to zirconia has been of great interest over the last 10–15 years. The aim of this study was to develop a zirconia bonding system and clarify its adhesion mechanism.MethodsA zirconia primer was prepared using tetra-n-propoxy zirconium (TPZr) and water. A silane primer was also prepared using γ-methacryloyloxypropyltrimethoxysilane (γ-MPS) and hydrochloric acid. After the zirconia primer was applied to the oxidized zirconia surface, the silane primer was applied to the ZrO2-functionalized layer and the resin cement was applied to the silane-modified layer. Ceramic Primer II was used as a typical MDP-based ceramic primer. Shear bond strengths were measured using a universal testing machine. To clarify the enhancing mechanism of the zirconia bonding system, X-ray photoelectron spectroscopy (XPS) analyses were performed.ResultsThe zirconia bond strength was affected by the surface wettability of zirconia, and the compositions of TPZr and water utilized in the zirconia primer. When the zirconia primer, consisting of 10μL TPZr and 13μL water, was applied to the zirconia surface that had been oxidized by H2O2 above 10%, the maximum bond strength of 8.2MPa was obtained. The mechanism of the zirconia bonding system was established as follows: the hydrolyzed zirconium species formed a more reactive ZrO2-functionalized layer on the oxidized zirconia surface, and the hydrolyzed γ-MPS species adsorbed on that layer introduces a chemical bonding to the resin.SignificanceThe novel zirconia bonding system enhanced the bonding performance of the resin, and showed a greater bond strength than an MDP-based ceramic primer.
http://ift.tt/2zfoxLy
Cytotoxicity and DNA double-strand breaks in human gingival fibroblasts exposed to eluates of dental composites
Source:Dental Materials
Author(s): Yang Yang, Franz-Xaver Reichl, Jianwei Shi, Xiuli He, Reinhard Hickel, Christof Högg
ObjectivePreviously, single composite components were used to study cytotoxicity and induction of DNA double-strand breaks (DNA-DSBs) of dental composite resins. In the present study, cytotoxicity and induction of DNA-DSBs in human gingival fibroblasts (HGFs) were investigated with dental composite eluates consisting of multiple components. The eluates were qualified and quantified.MethodsThe composites Esthet.X® HD, Venus®, X-tra fil®, CLEARFIL™ AP-X, Admira® Fusion and QuiXfil® were polymerized and immersed into Dulbecco's modified Eagle's medium (DMEM) for 72h. Subsequently, HGFs were incubated with the corresponding composite eluates. The cell viability of HGFs was obtained from an XTT assay. DNA-DSBs were determined using a γ-H2AX assay. The qualification and quantification of eluates were performed by gas chromatography/mass spectrometry (GC/MS).ResultsHGFs exposed to the eluates of all investigated composites showed no significant loss of cell viability, compared to negative control. Significant DNA-DSBs induction could be found in HGFs exposed to the eluates of Esthet.X® HD (0.43±0.05 foci/cell) and Venus® (0.39±0.04 foci/cell), compared to control (0.22±0.03 foci/cell). A total of 12 substances were detected from the investigated composite eluates. Five of them were methacrylates: tetraethyleneglycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA), hydroxypropyl methacrylate (HPMA), ethyleneglycol dimethacrylate (EGDMA) and trimethylolpropane trimethacrylate (TMPTMA). The highest concentration of HEMA (110.5μM), HPMA (86.08μM) and TMPTMA (4.50μM) was detected in the eluates of QuiXfil®. The highest concentration of TEGDMA was 1080μM in Venus® eluates and the highest concentration of EGDMA was 3.18μM in Esthet.X® HD eluates.SignificanceSignificant DNA-DSBs induction can be found in HGFs exposed to the eluates of Esthet.X® HD and Venus®. The interactive effects among released (co)monomers and additives may influence the cytotoxicity and induction of DNA-DSBs, compared to exposure with single composite component.
http://ift.tt/2iH242Z
Bioactivity and fluoride release of strontium and fluoride modified Biodentine
Source:Dental Materials
Author(s): Hazel O. Simila, Natalia Karpukhina, Robert G. Hill
Biodentine™ is a novel tricalcium silicate based material used both as a coronal dentine replacement and in pulp therapy. Its multiple use in sealing perforations, pulp capping and as a temporary restoration arises from its ability to promote dentine formation and to confer an excellent marginal seal. However, there is still room for improvement of this cement as it lacks the anticariogenic effect typically conferred by fluoride ion release as seen in glass ionomer cement based dental materials. Therefore, this study was conducted to investigate the impact of bioactive glass addition to Biodentine™.Objectivewas to compare the apatite formation capacity, specificity of the apatite type formed and fluoride ion release by Biodentine™ cements that have been modified by three different compositions of bioactive glasses.MethodsHigh fluoride, high strontium and high fluoride plus strontium containing bioactive glasses were synthesized, incorporated into Biodentine™ powder and four types of cements prepared. These cements were immersed in phosphate buffered saline solution and incubated for a period of 3 and 24h, 3, 7 and 14 days. Fourier transform infra-red spectroscopy, X-ray diffraction, magic angle spinning nuclear magnetic resonance and fluoride ion release studies were performed.ResultsBioactive glass addition to Biodentine™ led to pronounced formation of apatite. Where the bioactive glass contained fluoride, fluorapatite and fluoride ion release were demonstrated.SignificanceEliciting fluorapatite formation and fluoride ion release from Biodentine™ is an important development as fluoride is known to have antibacterial and anticariogenic effects.
http://ift.tt/2zeLONB
Instrument-assisted soft tissue mobilization increases myofascial trigger point pain threshold
Publication date: Available online 28 October 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Dawn T. Gulick
BackgroundA myofascial trigger point (MTrP) has been defined as a hyperirritable, palpable nodule in a skeletal muscle. The signs and symptoms of a MTrP include muscle pain, weakness, and dysfunction. MTrPs are common problems associated with soft tissue pathology. Having an intervention to decrease MTrP pain can be clinically valuable.PurposeTo determine if a series of six instrument-assisted soft tissue mobilization (IASTM) treatments rendered over three weeks would influence the pressure pain threshold (PPT) of a myofascial trigger point (MTrP).MethodsRandomized, control trial of healthy individuals (n = 29) with MTrPs in the upper trapezius muscle. The intervention was six IASTM treatments rendered over three weeks. Each treatment included 1 min of sweeping with the GT-1/HG-2 (handle bar), 1 min of swivel with the knob of the GT-1/HG-2 directly over the MTrP, 2 min of fanning with the GT-4/HG-8 (convex single bevel), and concluded with 1 min of sweeping with GT-1/HG-2.The outcome measure used a dolorimeter to compare PPT before and after three weeks in both the treatment and control groups.ResultsPaired t-test for PPT pre-test and post-test of the control and treatment groups were p = 0.42159 and p = 0.00003, respectively. A one-way ANOVA of the control and IASTM groups revealed a statistically significant difference (p < 0.0001). The power calculation was greater than 0.99.ConclusionsA 5-min intervention using three IASTM techniques can effectively increase the PPT of a MTrP in six treatments over a three-week period of time.
http://ift.tt/2z0VLx9
Standard classification and pathophysiology of rosacea: The 2017 update by the National Rosacea Society Expert Committee
In 2002, the National Rosacea Society assembled an expert committee to develop the first standard classification of rosacea. This original classification was intended to be updated as scientific knowledge and clinical experience increased. Over the last 15 years, significant new insights into rosacea's pathogenesis and pathophysiology have emerged, and the disorder is now widely addressed in clinical practice. Growing knowledge of rosacea's pathophysiology has established that a consistent multivariate disease process underlies the various clinical manifestations of this disorder, and the clinical significance of each of these elements is increasing as more is understood.
http://ift.tt/2iHtsOa
Sleep disturbance in children with moderate/severe atopic dermatitis: A case-control study
Sleep is disturbed in 60% of children with atopic dermatitis (AD).
http://ift.tt/2zfd8vh
Evidence-based update on rosacea comorbidities and their common physiologic pathways
Rosacea is a common chronic inflammatory disease affecting the facial skin whose etiology and pathophysiology are the subject of much investigation. Risk factors include genetic and environmental elements that may predispose individuals to localized inflammation and abnormal neurovascular responses to stimuli. Recent studies have introduced an array of systemic rosacea comorbidities, such as inflammatory bowel disease and neurologic conditions, that can be challenging to synthesize. We critically review the current data behind reported rosacea comorbidities and identify and highlight underrecognized physiologic mediators shared among rosacea and associated comorbidities.
http://ift.tt/2zf269t
Quantitative analysis of facial soft tissue perfusion during hypotensive anesthesia using laser-assisted indocyanine green fluorescence angiography
The aim of this study was to quantitatively evaluate the efficacy of induced hypotensive anesthesia in decreasing facial soft tissue perfusion during orthognathic surgery using laser-assisted indocyanine green fluorescence angiography. This retrospective study involved the evaluation of 16 patients who underwent orthognathic surgery. Data collection included facial tissue perfusion of the bilateral cheeks and chin at normotension and with pharmacologically induced hypotensive anesthesia. There were statistically significant differences in the facial tissue perfusion at normal and depressed levels of blood pressure (P<0.001).
http://ift.tt/2idFWcz
Treatment of lower lip pits in Van der Woude syndrome: a systematic review
The presence of lower lip pits in individuals with Van der Woude syndrome (VWS) may cause discomfort due to saliva secretion. Furthermore, one of the main complaints in relation to lip pits is poor aesthetics, which often affects quality of life. The aim of this systematic review was to identify the best technique for the surgical removal of lower lip pits in terms of aesthetic and functional characteristics. A search of the PubMed, Embase, Web of Science, Science Direct, and Scopus databases was performed on December 27, 2016, which retrieved 88 records without duplicates.
http://ift.tt/2zdAYra
Hyperlipidemia-induced hepassocin in the liver contributes to insulin resistance in skeletal muscle
Publication date: Available online 28 October 2017
Source:Molecular and Cellular Endocrinology
Author(s): Tae Woo Jung, Yoon Hee Chung, Hyoung-Chun Kim, A.M.Abd El-Aty, Ji Hoon Jeong
Hepassocin (HPS) has recently been identified as a novel hepatokine that causes hepatic steatosis. However, the role of HPS in the development of insulin resistance in skeletal muscle under obesity remains unclear. The effect of hyperlipidemia on hepatic HPS expression was evaluated in primary hepatocytes and liver of mice. HPS-mediated signal pathways were explored using small interfering (si) RNAs of specific genes or inhibitors. We found that treatment of primary hepatocytes with palmitate could induce HPS expression through C/EBPβ-mediated transcriptional activation. Furthermore, increased HPS expression was observed in the liver of high fat diet (HFD)-fed or tunicamycin-treated mice. Pretreatment with 4-phenylbutyrate (4-BPA) (an endoplasmic reticulum (ER) stress inhibitor) and suppression of p38 by siRNA abrogated the effect of palmitate on HPS expression in primary hepatocytes. Treatment of differentiated C2C12 cells with recombinant HPS caused c-Jun N-terminal kinase (JNK) phosphorylation and impairment of insulin sensitivity in a dose-dependent manner. siRNA-mediated suppression of JNK reduced the effect of HPS on insulin signaling. Furthermore, the suppression of epidermal growth factor receptor (EGFR) by siRNA mitigated both HPS-induced JNK phosphorylation and insulin resistance. In addition, HPS did not affect inflammation and ER stress in differentiated C2C12 cells. In conclusion, we elucidated that ER stress induced by palmitate could increase the expression of HPS in hepatocytes and further contribute to the development of insulin resistance in skeletal muscle via EGFR/JNK-mediated pathway. Taken together, we suggest that HPS could be a therapeutic target for obesity-linked insulin resistance.
Graphical abstract
http://ift.tt/2lmciGZ
Activin over-expression in the testis of mice lacking the inhibin α-subunit gene is associated with androgen deficiency and regression of the male reproductive tract
Source:Molecular and Cellular Endocrinology
Author(s): Rukmali Wijayarathna, David M. de Kretser, Andreas Meinhardt, Ralf Middendorff, Helen Ludlow, Nigel P. Groome, Kate A. Loveland, Mark P. Hedger
Regionalised interaction of the activins, follistatin and inhibin was investigated in the male reproductive tract of mice lacking the inhibin α-subunit (Inha−/-). Serum and intratesticular activin B, although not activin A and follistatin, were increased in Inha−/- mice at 25 days of age, but all three proteins were elevated at 56 days. None of these proteins were altered within the epididymis and vas deferens at either age. At 25 days, histology of the epididymis and vas deferens was similar to wild-type. At 56 days, the testis contained extensive somatic cell tumours, leading to Leydig cell regression and testosterone deficiency. The epididymis and vas deferens showed epithelial regression and increased prominence of the interstitial stroma. Immunoregulatory and fibrotic gene expression in the epididymis and vas deferens were unchanged. Thus, absence of the inhibin α-subunit has marginal effects on activins in the epididymis and vas deferens, and regression of these tissues is associated with androgen deficiency.
http://ift.tt/2lpc1Ty
Cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS)
Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome (CANVAS) is a novel ataxic disorder consisting of the triad of cerebellar impairment, bilateral vestibular hypofunction, and a somatosensory deficit. We report the first Japanese case of CANVAS. The patient is a 68-year-old Japanese male. He was referred to our university for further evaluation of progressive gait disturbance and ataxia. He exhibited horizontal gaze-evoked nystagmus and sensory deficit. Nerve conduction studies showed sensory neuronopathy.
http://ift.tt/2z0LRvy
A rare case of bilateral vagus nerve schwanomatosis
Schwanomatosis is the third most common form of neurofibromatosis. Schwanomatosis affecting the vagus nerve is particularly rare. In this report, we describe an extremely rare case bilateral vagus nerve schwanomatosis in a 45-year-old male patient. The patient initially presented with bilateral neck tumors and hoarseness arising after thoracic surgery. We performed left neck surgery in order to diagnose and resect the remaining tumors followed by laryngeal framework surgery to improve vocal cord closure and symptoms of hoarseness.
http://ift.tt/2yVVLNc
Cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS)
Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome (CANVAS) is a novel ataxic disorder consisting of the triad of cerebellar impairment, bilateral vestibular hypofunction, and a somatosensory deficit. We report the first Japanese case of CANVAS. The patient is a 68-year-old Japanese male. He was referred to our university for further evaluation of progressive gait disturbance and ataxia. He exhibited horizontal gaze-evoked nystagmus and sensory deficit. Nerve conduction studies showed sensory neuronopathy.
http://ift.tt/2z0LRvy
A rare case of bilateral vagus nerve schwanomatosis
Schwanomatosis is the third most common form of neurofibromatosis. Schwanomatosis affecting the vagus nerve is particularly rare. In this report, we describe an extremely rare case bilateral vagus nerve schwanomatosis in a 45-year-old male patient. The patient initially presented with bilateral neck tumors and hoarseness arising after thoracic surgery. We performed left neck surgery in order to diagnose and resect the remaining tumors followed by laryngeal framework surgery to improve vocal cord closure and symptoms of hoarseness.
http://ift.tt/2yVVLNc
Morphology and Outcomes of Total Endovascular Treatment of Type B Aortic Dissection with Aberrant Right Subclavian Artery
Publication date: Available online 28 October 2017
Source:European Journal of Vascular and Endovascular Surgery
Author(s): Min Zhou, Xueqin Bai, Yong Ding, Yonggang Wang, Changpo Lin, Dong Yan, Zhenyu Shi, Weiguo Fu
ObjectivesTo characterize the morphology of type B aortic dissection with aberrant right subclavian artery (ARSA) and present early and midterm outcomes of total endovascular treatment for affected patients.MethodsFrom January 2010 to December 2015, patients with ARSA and type B aortic dissection treated with total endovascular techniques were enrolled. The angle of the aortic arch was measured on pre-operative CTA. Sixty age and gender matched normal aortic arch patients with type B aortic dissection served as controls. Primary outcomes were technical success, 30 day mortality, and late survival. Secondary outcomes included in hospital morbidity, re-intervention rate, and patency of the subclavian artery.ResultsA total of 13 patients (8 men, 5 women; mean age 58 years) were included. The mean angle of the aortic arch in patients with ARSA was significantly smaller than in normal aortic arch patients (117.2° ± 10.8° vs. 124.2° ± 9.4°, respectively; p = .024). Simple thoracic endovascular aortic repair (TEVAR) and TEVAR plus a parallel graft technique were performed in six and seven patients, respectively. Primary technique success was achieved in 11 of the 13 (84.6%) patients. A bird beak configuration occurred significantly more frequently in patients with ARSA than in normal aortic arch patients (91.7% vs. 48.3%, respectively; p = .035). The median follow-up time was 36 months. One patient received a secondary procedure because of a new onset entry tear at the distal end of the stent graft. No posterior circulation stroke, permanent spinal cord ischaemia, or ischaemia of the upper arm was observed.ConclusionsType B aortic dissection with ARSA was associated with a steep aortic arch. Total endovascular treatment for these patients was feasible and safe. Stent grafts with better flexibility and appropriate extension of the proximal landing zone with a parallel graft technique are suggested based on the observed outcomes.
http://ift.tt/2zewyjN
Determinants of Acute Kidney Injury and Renal Function Decline After Endovascular Abdominal Aortic Aneurysm Repair
Publication date: Available online 27 October 2017
Source:European Journal of Vascular and Endovascular Surgery
Author(s): Randolph G. Statius van Eps, Banne Nemeth, Ronne T.A. Mairuhu, Jan J. Wever, Hugo T.C. Veger, Hans van Overhagen, Lukas C. van Dijk, Bob Knippenberg
Objective/BackgroundEndovascular aneurysm repair (EVAR) may be associated with renal injury and more insight is needed into potential risk factors. The aim was to identify clinical, anatomical, and peri-procedural parameters as potential risk factors for the occurrence of acute kidney injury (AKI) and to evaluate chronic kidney disease (CKD) after EVAR.MethodsA cohort of 212 consecutive patients who underwent elective EVAR for abdominal aortic aneurysm from January 2009 to October 2016 was included. A subgroup of 149 patients with 2 years follow-up was compared with a set of 135 non-operated aneurysm patients with smaller aneurysms (similar cardiovascular risk profile) to assess CKD. Primary outcomes were AKI (Acute Kidney Injury Network criteria) and CKD measured by estimated glomerular filtration rate (Kidney Disease Improving Global Outcomes guidelines). For AKI, candidate risk factors were identified by univariate and multivariate logistic regression analysis; for chronic renal function decline, risk factors were identified using Cox regression analysis.ResultsAKI occurred in 30 patients (15%). On multivariate analysis, the use of angiotensin II blocker (odds ratio [OR] 4.08, 95% confidence interval [CI] 1.38–12.07) and peri-operative complications (OR 3.12, 95% CI 1.20–8.10) were independent risk factors for AKI, whereas statin use was a protective factor (OR 0.19, 95% CI 0.07–0.52). EVAR resulted in a significant increase (23.5%) in the occurrence of CKD compared with the control group (6.7%; p <.001). On univariate and multivariate Cox regression the risk factors: aortic neck diameter (per mm increase) (hazard ratio [HR] 1.13, 95% CI 1.02–1.25), renal artery stenosis >50% (HR 2.24, 95% CI 1.05–4.79), and the occurrence of AKI (HR 2.19, 95% CI 0.99–4.85) were significant predictors of CKD.ConclusionThis study identified use of angiotensin II blockers and peri-operative complications as risk factors for AKI. In addition, the problem of renal function decline after EVAR is highlighted, which indicates that prolonged protective measures (e.g., in those patients at high risk) over time are needed to improve patient outcomes.
http://ift.tt/2iJZgSz
Improving the Efficiency of Speech-In-Noise Hearing Screening Tests
http://ift.tt/2hihd7q
Rotational and Collic Vestibular-Evoked Myogenic Potential Testing in Normal Developing Children and Children With Combined Attention Deficit/Hyperactivity Disorder
http://ift.tt/2zPahoW
Systemic Aminoglycosides-Induced Vestibulotoxicity in Humans
http://ift.tt/2zPafNQ
Comparison of the Spectral-Temporally Modulated Ripple Test With the Arizona Biomedical Institute Sentence Test in Cochlear Implant Users
http://ift.tt/2zPaeJM
Rehabilitation and Psychosocial Determinants of Cochlear Implant Outcomes in Older Adults
http://ift.tt/2zPad8G
Age-Related Differences in the Processing of Temporal Envelope and Spectral Cues in a Speech Segment
http://ift.tt/2zPacBE
The Effect of Aging and Priming on Same/Different Judgments Between Text and Partially Masked Speech
http://ift.tt/2zPaaK2
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- An Evaluation of Different Bridging Techniques for...
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- Editorial Board
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- Editorial Board
- Reprint of “Dual blockade of the pro-inflammatory ...
- The influence of continental air masses on the aer...
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