Head and Neck Diseases by Alexandros G.Sfakianakis: 02/21/16

Κυριακή 21 Φεβρουαρίου 2016

The new issue is now available.Nippon Daicho Komonbyo Gakkai Zasshi

Vol.69 No.3

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The new issue is now available.Plankton and Benthos Research

Vol.11 No.1

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The new issue is now available.Journal of Japan Society of Civil Engineers, Ser. A2 (Applied Mechanics (AM))

Vol.71 No.2

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The new issue is now available.GASTROENTEROLOGICAL ENDOSCOPY

Vol.58 No.2

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Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.

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Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Primary cilia influence various signaling pathways. The mammalian cochlea is ideal for examining planar cell polarity (PCP) signaling. Cilia dysfunction affects cochlear outgrowth, cellular patterning and hair cell orientation, readouts of PCP. Our goal is to analyze PCP signaling in mouse cochlea via phenotypic analysis, immunohistochemistry and scanning electron microscopy.

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Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

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Aeromicrobium halotolerans sp. nov., isolated from desert soil sample

Abstract

A Gram-positive, aerobic, and non-motile, rod-shaped actinomycete strain, designated YIM Y47^T, was isolated from soils collected from Turpan desert, China, and subjected to a polyphasic taxonomic study. Phylogenetic analysis indicated that strain YIM Y47^T belonged to the genus Aeromicrobium. YIM Y47^T shared highest 16S rRNA gene sequence similarities with Aeromicrobium massiliense JC14^T (96.47 %). Growth occurs at 20–45 °C (optimum at 30 °C), pH 6.0–8.0 (optimum at pH 7.0), and salinities of 0–7.0 % NaCl (optimum at 4.0 %). The strain YIM Y47^T exhibits chemotaxonomic features with menaquinone-7 (MK-7) as the predominant quinone, C_16:0, C_18:1ω9c and 10-methyl C_18:0 (>10 %) as major fatty acids. The cell-wall peptidoglycan of strain YIM Y47^T contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and unknown phospholipids. The G+C content of the genomic DNA of strain YIM Y47^T was found to be 44.7 mol%. On the basis of phylogenetic analyses and phenotypic data, it is proposed that strain YIM Y47^T should be classified as representing a novel species of the genus Aeromicrobium, with the name Aeromicrobium halotolerans sp. nov. The type strain is YIM Y47^T (=KCTC 39113^T=CGMCC 1.15063^T=DSM 29939^T=JCM 30627^T).

Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus

Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were used to isolate and identify two major constituents of an active fraction (Fraction F) obtained from the medium. Compound 1 was identified as 2-hydroxypropane-1, 2, 3-tricarboxylic acid (citric acid). Compound 2 was identified as 3-hydroxy-5-methoxy-3-(methoxycarbonyl)-5-oxopentanoic acid, an isomer of 1, 2-dimethyl citrate. Compound 1 and a citric acid standard, each tested at 50 mg mL^–1 in water, decreased hatch from eggs of the plant-parasitic nematode Meloidogyne incognita by more than 94%, and completely immobilized second-stage juveniles after 4–6 days exposure. Fraction F and Compounds 1 and 2 decreased the mobility of adults of the plant-parasitic nematode Ditylenchus destructor in vitro. Fraction F (25 mg mL^–1) inhibited mobility >99% at 72 hrs. Compounds 1 and 2 (50 mg mL^–1) each inhibited mobility more than 25% at 24 hr and more than 50% at 72 hr. This is the first assignment of nematode-antagonistic properties to specifically identified A. candidus metabolites.

Permissiveness of freshly isolated environmental strains of amoebae for growth of Legionella pneumophila

Legionella pneumophila is a pathogenic bacterium commonly found in water and responsible for severe pneumonia. Free-living amoebae are protozoa also found in water, which feed on bacteria by phagocytosis. Under favorable conditions, some L. pneumophila are able to resist phagocytic digestion and even multiply within amoebae. However, it is not clear whether L. pneumophila could infect at a same rate a large range of amoebae or if there is some selectivity towards specific amoebal genera or strains. Also, most studies have been performed using collection strains and not with freshly isolated strains. In our study, we assess the permissiveness of freshly isolated environmental strains of amoebae, belonging to three common genera (i.e. Acanthamoeba, Naegleria and Vermamoeba), for growth of L. pneumophila at three different temperatures. Our results indicated that all the tested strains of amoebae were permissive to L. pneumophila Lens and that there was no significant difference between the strains. Intracellular proliferation was more efficient at a temperature of 40°C. In conclusion, our work suggests that, under favorable conditions, virulent strains of L. pneumophila could equally infect a large number of isolates of common freshwater amoeba genera.

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Development of a removable head fixation device for longitudinal behavioral and imaging studies in rats

Publication date: Available online 21 February 2016
Source:Journal of Neuroscience Methods
Author(s): Yuki Hori, Jun Ogura, Naoki Ihara, Tsunehisa Higashi, Takayuki Tashiro, Manabu Honda, Takashi Hanakawa
BackgroundIn some behavioral neuroscience studies, an attachment is surgically fixed onto the head of an awake animal to allow the animal to perform learning tasks repeatedly in the same position in a task-training system. A recently developed task-training system enables operant conditioning of head-fixed rats within only a few days, and this system has been rigorously applied to record learning-associated neural activity using electrophysiological techniques. However, the head attachment of this device is made of metal and thus is not suitable for simultaneous brain imaging studies with X-ray computed tomography (CT), magnetic resonance imaging (MRI) or positron emission tomography (PET).New MethodWe developed a novel head fixation device with a removable attachment to position the rat head precisely in both imaging and training devices across different sessions. The device consisted of a removable attachment, a clamp and a stage, all of which were made of PET/MRI compatible acrylic resin. We tested the usefulness of the device with 18F-fluorodeoxyglucose (FDG) PET and CT.ResultsThe new device did not substantially affect 18F-FDG PET images. Repositioning of the rat's head across sessions and experimenters was at a level of submillimeter accuracy.Comparison with Existing MethodThe errors of radioactivity concentration of 18F- FDG in the PET image were lower with the present attachment than with the conventional metal attachment. Repositioning accuracy was considerably improved compared with a visual inspection method.ConclusionsThe developed fixation device is useful for longitudinal behavioral and brain imaging studies in rats.

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Development of a removable head fixation device for longitudinal behavioral and imaging studies in rats

Publication date: Available online 21 February 2016
Source:Journal of Neuroscience Methods
Author(s): Yuki Hori, Jun Ogura, Naoki Ihara, Tsunehisa Higashi, Takayuki Tashiro, Manabu Honda, Takashi Hanakawa
BackgroundIn some behavioral neuroscience studies, an attachment is surgically fixed onto the head of an awake animal to allow the animal to perform learning tasks repeatedly in the same position in a task-training system. A recently developed task-training system enables operant conditioning of head-fixed rats within only a few days, and this system has been rigorously applied to record learning-associated neural activity using electrophysiological techniques. However, the head attachment of this device is made of metal and thus is not suitable for simultaneous brain imaging studies with X-ray computed tomography (CT), magnetic resonance imaging (MRI) or positron emission tomography (PET).New MethodWe developed a novel head fixation device with a removable attachment to position the rat head precisely in both imaging and training devices across different sessions. The device consisted of a removable attachment, a clamp and a stage, all of which were made of PET/MRI compatible acrylic resin. We tested the usefulness of the device with ¹⁸F-fluorodeoxyglucose (FDG) PET and CT.ResultsThe new device did not substantially affect ¹⁸F-FDG PET images. Repositioning of the rat's head across sessions and experimenters was at a level of submillimeter accuracy.Comparison with Existing MethodThe errors of radioactivity concentration of ¹⁸F- FDG in the PET image were lower with the present attachment than with the conventional metal attachment. Repositioning accuracy was considerably improved compared with a visual inspection method.ConclusionsThe developed fixation device is useful for longitudinal behavioral and brain imaging studies in rats.

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IMPORTANCE OF SERATONIN GENE RELATED PEPTIDE (SGRP) IN MIGRAINE

2016-02-21T11-56-54Z
Source: International Journal of Health and Rehabilitation Sciences (IJHRS)
Sivanandan Ramar, Sankaran Ponnusamy, Salameh Al Dajah.
Migraine is a neurovascular disorder involving trigeminal ganglion characterized by recurrent episodic headache and rise in levels of Serotonin Gene Related Protein (SGRP) in plasma. SGRP is a neuropeptide present in the central and peripheral nervous system that has diverse functions as primary afferent neurotransmitter which is important in nociception. In this study expression of SGRP studied in neurons of trigeminal ganglion in male wistar albino rats. SGRP is expressed in cytoplasm of neurons mainly in the small sized neurons indicating that small sized neurons are mainly involved in nociception. Keyword:.

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Development of a removable head fixation device for longitudinal behavioral and imaging studies in rats

Publication date: Available online 21 February 2016
Source:Journal of Neuroscience Methods
Author(s): Yuki Hori, Jun Ogura, Naoki Ihara, Tsunehisa Higashi, Takayuki Tashiro, Manabu Honda, Takashi Hanakawa
BackgroundIn some behavioral neuroscience studies, an attachment is surgically fixed onto the head of an awake animal to allow the animal to perform learning tasks repeatedly in the same position in a task-training system. A recently developed task-training system enables operant conditioning of head-fixed rats within only a few days, and this system has been rigorously applied to record learning-associated neural activity using electrophysiological techniques. However, the head attachment of this device is made of metal and thus is not suitable for simultaneous brain imaging studies with X-ray computed tomography (CT), magnetic resonance imaging (MRI) or positron emission tomography (PET).New MethodWe developed a novel head fixation device with a removable attachment to position the rat head precisely in both imaging and training devices across different sessions. The device consisted of a removable attachment, a clamp and a stage, all of which were made of PET/MRI compatible acrylic resin. We tested the usefulness of the device with ¹⁸F-fluorodeoxyglucose (FDG) PET and CT.ResultsThe new device did not substantially affect ¹⁸F-FDG PET images. Repositioning of the rat's head across sessions and experimenters was at a level of submillimeter accuracy.Comparison with Existing MethodThe errors of radioactivity concentration of ¹⁸F- FDG in the PET image were lower with the present attachment than with the conventional metal attachment. Repositioning accuracy was considerably improved compared with a visual inspection method.ConclusionsThe developed fixation device is useful for longitudinal behavioral and brain imaging studies in rats.

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Tutoring Trainees to Suture: An Alternative Method for Learning How to Suture and a Way to Compensate for a Lack of Suturing Cases

Publication date: Available online 20 February 2016
Source:Journal of Surgical Education
Author(s): Apinut Wongkietkachorn, Peera Rhunsiri, Pangpoom Boonyawong, Attaporn Lawanprasert, Kasaya Tantiphlachiva
IntroductionTutoring in suturing was developed to compensate for a shortage of suturing cases. The objective of this study was to compare ideal suturing score (ISS; 9 points), suturing time (min:sec), and suture placement error (mm) between medical students completing the suturing tutoring program and medical students attending ordinary medical school training program.MethodsParticipants consisted of 2 groups of medical students who had never performed suturing. The study group had the role of suturing tutor to teach interested high school students. The control group consisted of volunteers from the ordinary medical school program. Skills measurement was performed by having students from both the groups perform 3 vertical mattress sutures on a model. The study group was tested at weeks 1, 9, and 10 to assess improvement. Both the groups were tested at week 10 to compare final learning outcome.ResultsThere were 41 and 40 participants in the study group and the control group, respectively. ISS was significantly improved in the study group from week 1-week 10 (7.0 ± 1.3 vs. 8.2 ± 0.9, p = 0.01). At week 10, the study group had a higher mean ISS than the control group (8.2 ± 0.9 vs. 7.8 ± 1.1, p = 0.68). Mean suturing time and mean placement error were also lower in the study group at the end of suturing training (5:1 ± 1:0 vs. 5:2 ± 1:2, p = 0.13; 7.4 ± 7.4 vs. 8.0 ± 10.8, p = 0.44).ConclusionTutoring trainees to suture can improve a student's ability to learn how to suture.

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The new issue is now available.Nippon Daicho Komonbyo Gakkai Zasshi

Vol.69 No.3

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The new issue is now available.KAKUIGAKU

Vol.53 No.1

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The new issue is now available.The Japanese Journal of Dermatology

Vol.126 No.2

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The new issue is now available.Journal of Japan Society of Civil Engineers, Ser. A2 (Applied Mechanics (AM))

Vol.71 No.2

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The new issue is now available.GASTROENTEROLOGICAL ENDOSCOPY

Vol.58 No.2

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The new issue is now available.GASTROENTEROLOGICAL ENDOSCOPY

Vol.58 No.2

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The new issue is now available.Journal of Japan Society of Civil Engineers, Ser. A2 (Applied Mechanics (AM))

Vol.71 No.2

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The new issue is now available.Plankton and Benthos Research

Vol.11 No.1

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The new issue is now available.Plankton and Benthos Research

Vol.11 No.1

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The Mental Health of Elite Athletes: A Narrative Systematic Review

Abstract

Background

The physical impacts of elite sport participation have been well documented; however, there is comparatively less research on the mental health and psychological wellbeing of elite athletes.

Objective

This review appraises the evidence base regarding the mental health and wellbeing of elite-level athletes, including the incidence and/or nature of mental ill-health and substance use.

Methods

A systematic search of the PubMed, EMBASE, SPORTDiscus, PsycINFO, Cochrane and Google Scholar databases, up to and including May 2015, was conducted.

Results

The search yielded a total of 2279 records. Following double screening, 60 studies were included. The findings suggested that elite athletes experience a broadly comparable risk of high-prevalence mental disorders (i.e. anxiety, depression) relative to the general population. Evidence regarding other mental health domains (i.e. eating disorders, substance use, stress and coping) is less consistent. These results are prefaced, however, by the outcome of the quality assessment of the included studies, which demonstrated that relatively few studies (25 %) were well reported or methodologically rigorous. Furthermore, there is a lack of intervention-based research on this topic.

Conclusion

The evidence base regarding the mental health and wellbeing of elite athletes is limited by a paucity of high-quality, systematic studies. Nonetheless, the research demonstrates that this population is vulnerable to a range of mental health problems (including substance misuse), which may be related to both sporting factors (e.g. injury, overtraining and burnout) and non-sporting factors. More high-quality epidemiological and intervention studies are needed to inform optimal strategies to identify and respond to player mental health needs.

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The new issue is now available.Oukan (Journal of Transdisciplinary Federation of Science and Technology)

Mini Special Issue on Post-Disaster Recovery and Reconstruction from the Viewpoint of Socio-Informatics

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The new issue is now available.Oukan (Journal of Transdisciplinary Federation of Science and Technology)

Mini Special Issue on Post-Disaster Recovery and Reconstruction from the Viewpoint of Socio-Informatics

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Ansia o altro? – Medicitalia.it (Comunicati Stampa) (Registrazione) (Blog)

Ansia o altro?
Medicitalia.it (Comunicati Stampa) (Registrazione) (Blog)
Sono stata meglio. Circa due settimane fa ho avuto invece un paio di episodi (a distanza di qualche giorno) di leggero giramento di testa (durato un paio di secondi) con ronzio alle orecchie e sensazione di calore ai piedi. Mi è successo da seduta e …

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IFC continued (editorial board)

Publication date: 30 March 2016
Source:Toxicology Letters, Volume 246

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A Review on Animal Models of Stroke: an Update

Publication date: Available online 21 February 2016
Source:Brain Research Bulletin
Author(s): Anil Kumar, Aakriti, Varun Gupta
Stroke is one of the major healthcare challenges prevailing across the globe due to its significant rate of mortality and morbidity. Stroke is multifactorial in nature and involves several cellular and molecular signalling cascades that make the pathogenesis complex and treatment difficult. For a deeper understanding of the diverse pathological mechanisms and molecular & cellular cascades during stroke, animal modelling serves as a reliable and an effective tool. This also helps to develop and critically analyse various neuroprotective strategies for the mitigation of this devastating disease. Animal modelling for stroke has been revolutionized with the development of newer and more relevant models or approaches that mimic the clinical setting of stroke to a greater extent. This review analyses experimental models of stroke (ischemic and hemorrhagic) and their reliability in stroke situation. Besides this, the review also stresses upon the use of various preclinical models to understand the pathophysiological mechanisms that operate during stroke and to elucidate new, safe and effective neuroprotective agents to combat this life threatening healthcare concern.

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The new issue is now available.Oukan (Journal of Transdisciplinary Federation of Science and Technology)

Mini Special Issue on "Timeaxis Design" as a Transdisciplinary Approach

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The Effectiveness of Manual versus Algometer Pressure Release Techniques for Treating Active Myofascial Trigger Points of the Upper Trapezius

Publication date: Available online 21 February 2016
Source:Journal of Bodywork and Movement Therapies
Author(s): Walaa Abu-Taleb, Aliaa Rehan Youssef, Amir Saleh
Manual pressure release (MPR) is a popular treatment of trigger points. Yet, treatment response may be influenced by inconsistent application of pressure. Further, it may contribute to increased risk of work-related musculoskeletal disorders of the wrist and hand in therapists. Therefore, this study aimed at introducing a novel method to apply pressure using the algometer and to compare its effectiveness to MPR. Forty-five volunteers with active trigger points of the upper trapezius received algometer pressure release (APR), MPR, or sham ultrasound (US). Pain pressure threshold (PPT) and contralateral active and passive neck side-bending ranges were assessed at baseline and immediately after a single session. Results showed no significant differences in post-treatment PPT between the study groups (p>0.05). The APR group showed a significant increase in passive side-bending range compared with the two other groups, whereas active range improved in the APR compared with the US group (p<0.05). Our results show that using APR to apply pressure release to upper trapezius trigger points is more effective compared with manual release and sham US.

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Acute PID-MRI

Clinical Data: Patient presented with 2 days history of pain abdomen and fever. Clinical examination revealed lump in right adnexa.

MRI showed a heterogenous area abutting the uterus on right side extending between uterus & right ovary possibly indicating acute inflammatory tubal lesion. Fluid in POD.

This patient likely requires follow up after a course of intravenous antibiotics.

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CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

CD97/ADGRE5 protein is predominantly expressed on leukocytes and belongs to the EGF-TM7 receptors family. It mediates granulocytes accumulation in the inflammatory tissues and is involved in firm adhesion of PMNC on activated endothelial cells. There have not been any studies exploring the role of CD97 in LPS induced NF-κB activation in macrophages. Therefore, we first measured the CD97 expression in LPS treated human primary macrophages and subsequently analyzed the levels of inflammatory factor TNF-α and transcription factor NF-κB in these macrophages that have been manipulated with either CD97 knockdown or overexpression. We found that a reported anti-inflammatory transcription factor, PPAR-γ, was involved in the CD97 mediated NF-κB suppression. Furthermore, by immunofluorescence staining, we established that CD97 overexpression not only inhibited LPS induced p65 expression in the nucleus but also promoted the PPAR-γ expression. Moreover, using CD97 knockout THP-1 cells, we further demonstrated that CD97 promoted PPAR-γ expression and decreased LPS induced NF-κB activation. In conclusion, CD97 plays a negative role in LPS induced NF-κB activation and TNF-α secretion, partly through PPAR-γ upregulation.

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Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method

It is postulated that biofilm formation in the oral cavity causes some oral diseases. Lactoferrin is an antibacterial protein in saliva and an important defense factor against biofilm development. We analyzed the adsorbed amount of lactoferrin and the dissociation constant (Kd) of lactoferrin to the surface of different dental materials using an equilibrium analysis technique in a 27 MHz quartz crystal microbalance (QCM) measurement. Four different materials, titanium (Ti), stainless steel (SUS), zirconia (ZrO2) and polymethyl methacrylate (PMMA), were evaluated. These materials were coated onto QCM sensors and the surfaces characterized by atomic force microscopic observation, measurements of surface roughness, contact angles of water, and zeta potential. QCM measurements revealed that Ti and SUS showed a greater amount of lactoferrin adsorption than ZrO2 and PMMA. Surface roughness and zeta potential influenced the lactoferrin adsorption. On the contrary, the Kd value analysis indicated that the adsorbed lactoferrin bound less tightly to the Ti and SUS surfaces than to the ZrO2 and PMMA surfaces. The hydrophobic interaction between lactoferrin and ZrO2 and PMMA is presumed to participate in better binding of lactoferrin to ZrO2 and PMMA surfaces. It was revealed that lactoferrin adsorption behavior was influenced by the characteristics of the material surface.

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Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

The primary sheep trophoblast cells (STCs) have a finite lifespan in culture. This feature limits the scope for long-term in vitro studies with STCs. This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line. STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively. The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs). hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence. Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunit β (CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs. Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells. hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice. In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.

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A Simple Allergist-Led Intervention Improves Resident Training in Anaphylaxis

Physicians underrecognize and undertreat anaphylaxis. Effective interventions are needed to improve physician knowledge and competency regarding evidence-based anaphylaxis diagnosis and management (ADAM). We designed and evaluated an educational program to improve ADAM in pediatrics, internal medicine, and emergency medicine residents from two academic medical centers. Anonymous questionnaires queried participants' demographics, prior ADAM clinical experience, competency, and comfort. A pretest assessing baseline knowledge preceded a 45-minute allergist-led evidence-based presentation, including practice with epinephrine autoinjectors, immediately followed by a posttest. A follow-up test assessed long-term knowledge retention twelve weeks later. 159 residents participated in the pretest, 152 participated in the posttest, and 86 participated in the follow-up test. There were no significant differences by specialty or site. With a possible score of 10, the mean pretest score (7.31 ± 1.50) was lower than the posttest score (8.79 ± 1.29) and follow-up score (8.17 ± 1.72) ( for both). Although participants' perceived confidence in diagnosing or managing anaphylaxis improved from baseline to follow-up ( for both), participants' self-reported clinical experience with ADAM or autoinjector use was unchanged. Allergist-led face-to-face educational intervention improves residents' short-term knowledge and perceived confidence in ADAM. Limited clinical experience or reinforcement contributes to the observed decreased knowledge.

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TWiV 377: Chicken with a side of Zika

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove,

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CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

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Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method

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Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174

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Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

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Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174

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Cytogenetic Studies of Rwandan Pediatric Patients Presenting with Global Developmental Delay, Intellectual Disability and/or Multiple Congenital Anomalies

In conclusion, our results highlight the high frequency of cytogenetically detectable abnormalities in this series, with implications for the burden on the healthcare. This study demonstrates the importance of cytogenetic analysis in patients with GDD/ID and MCA. (Source: Journal of Tropical Pediatrics)

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Fukushima: 16 more cases of child thyroid cancer linked to nuclear disaster – International Business Times UK

International Business Times UK

Fukushima: 16 more cases of child thyroid cancer linked to nuclear disaster
International Business Times UK
The second follow-up survey of the 2011 Fukushima disaster has thrown up more diagnoses of children with thyroid cancer. Since April 2014, 16 children have been confirmed to have the condition, as well as 35 suspected cases, all aged between six and …
At least 16 children confirmed to have thyroid cancer in second Fukushima surveySouth China Morning Post (subscription)
16 children confirmed to have thyroid cancer and 35 suspected of having disease in 2nd Fukushima surveyCitizens for Legitimate Government
Fukushima confirms 1 additional thyroid cancer caseAsahi Shimbun
Japan Today
all 6 news articles »

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Ultrasonic vocalization in murine experimental stroke: A mechanistic model of aphasia.

CONCLUSION: These findings set a foundation for further studies of mechanisms and novel treatment strategies for post-stroke vocalization impairments. PMID: 26889967 [PubMed – as supplied by publisher] (Source: Restorative Neurology and Neuroscience)

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Information Analysis of Catchment Hydrologic Patterns across Temporal Scales

Catchment hydrologic cycle takes on different patterns across temporal scales. The interim between event-scale hydrologic process and mean annual water-energy correlation pattern requires further examination to justify self-consistent understanding. In this paper, the temporal scale transition revealed by observation and simulation was evaluated in an information theoretical framework named Aleatory Epistemic Uncertainty Estimation. The Aleatory Uncertainty refers to posterior uncertainty of runoff given the input variables' observations. The Epistemic Uncertainty refers to the posterior uncertainty increase due to the imperfect observation decoding in models. Daily hydrometeorological observations in 24 catchments were aggregated from 10 days to 1 year before implementing the information analysis. Estimations of information contents and flows of hydrologic terms across temporal scales were related with the catchments' seasonality type. It also showed that information distilled by the monthly and annual water balance models applied here did not correspond to that provided by observations around temporal scale from two months to half a year. This calls for a better understanding of seasonal hydrologic mechanism.

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Modeling Financial Time Series Based on a Market Microstructure Model with Leverage Effect

The basic market microstructure model specifies that the price/return innovation and the volatility innovation are independent Gaussian white noise processes. However, the financial leverage effect has been found to be statistically significant in many financial time series. In this paper, a novel market microstructure model with leverage effects is proposed. The model specification assumed a negative correlation in the errors between the price/return innovation and the volatility innovation. With the new representations, a theoretical explanation of leverage effect is provided. Simulated data and daily stock market indices (Shanghai composite index, Shenzhen component index, and Standard and Poor's 500 Composite index) via Bayesian Markov Chain Monte Carlo (MCMC) method are used to estimate the leverage market microstructure model. The results verify the effectiveness of the model and its estimation approach proposed in the paper and also indicate that the stock markets have strong leverage effects. Compared with the classical leverage stochastic volatility (SV) model in terms of DIC (Deviance Information Criterion), the leverage market microstructure model fits the data better.

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Nonlinear Modeling and Dynamic Simulation Using Bifurcation and Stability Analyses of Regenerative Chatter of Ball-End Milling Process

A dynamic model for a ball-end milling process that includes the consideration of cutting force nonlinearities and regenerative chatter effects is presented. The nonlinear cutting force is approximated using a Fourier series and then expanded into a Taylor series up to the third order. A series of nonlinear analyses was performed to investigate the nonlinear dynamic behavior of a ball-end milling system, and the differences between the nonlinear analysis approach and its linear counterpart were examined. A bifurcation analysis of points near the critical equilibrium points was performed using the method of multiple scales (MMS) and the method of harmonic balance (MHB) to analyse the local chatter behaviors of the system. The bifurcation analysis was conducted at two subcritical Hopf bifurcation points. It was also found that a ball-end milling system with nonlinear cutting forces near its critical equilibrium points is conditionally stable. The analysis and simulation results were compared with experimental data reported in the literature, and the physical significance of the results is discussed.

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Sustained virological response after a 17-day treatment with daclatasvir plus asunaprevir in a cirrhotic patient with hepatitis C virus genotype 1b and null response for peginterferon ribavirin therapy

Abstract

Daclatasvir (DCV) plus asunaprevir (ASV) treatment, an oral therapy for chronic hepatitis C virus (HCV) genotype 1b infection, can achieve a high sustained viral response (SVR) rate within a 24-week treatment period. A 55-year-old Japanese female with cirrhosis and null response for peginterferon plus ribavirin therapy received DCV plus ASV therapy, but she reported a slight fever beginning on treatment day 4. The fever increased to >38.0 °C beginning on treatment day 15 and could not be controlled with antipyretics; thus, the treatment was discontinued on day 17. Although the patient was still positive for HCV RNA 6 days after treatment discontinuation, she achieved an SVR at week 24 after treatment cessation. In some patients with HCV genotype 1b infection, an SVR can be achieved with short-term DCV plus ASV treatment, and HCV RNA positivity at the end of treatment does not always indicate virological failure.

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Practice patterns in the intraoperative use of bispectral index monitoring

Abstract

Assessing the depth of anesthesia and reducing intraoperative awareness has become a focus of much technology development and research in the field of anesthesia. Bispectral index (BIS) is the most widely utilized technology that uses electroencephalogram to provide a measurement of anesthetic depth. There are no definitive guidelines on when BIS should be used. Our aim was to assess actual patterns of intraoperative use of BIS by anesthesia professionals. We retrospectively collected intraoperative data on 55,210 surgical cases at a tertiary care hospital. Variables collected included: age, sex, body mass index (BMI), American Society of Anesthesiologists (ASA) physical status, anesthesia provider type and level of training, use of inhalational anesthetics versus total intravenous anesthesia (TIVA), utilization of nitrous oxide, utilization of non-depolarizing neuromuscular blockade, emergency status of surgery, airway type, case duration, and surgical subspecialty. A univariate logistic regression model was fitted. Subsequently, a multivariate logistic regression model was applied. Covariates utilized for the model included age, anesthesia provider level, and length of case. Factors associated with BIS use included increased age, greater ASA physical status, extremes of BMI, use of TIVA, use of a long-acting paralytic agent, use of an endotracheal tube (ETT), emergency surgery, increasing length of case, and certain surgical services. BIS use was associated with previously documented risk factors for intraoperative awareness. These factors are also indicators of case complexity, which may be a major factor among providers deciding when to apply BIS monitoring in the operating room.

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A Review on Animal Models of Stroke: an Update

Pediatric Stroke-MRI

Four old child presented with sudden unconsciousness since 2 days. MRI revealed multifocal T2/FLAIR hyperintense areas in the right thalamic/occipital region-PCA territory, left perisylvian (MCA territory) and left inferior cerebellar hemisphere showing areas of restricted diffusion on DWI and corresponding ADC maps. These findings likely indicate areas of relatively fresh infarction in multifocal vascular territories. Differentials include-causes like hypercoaguable state, vasculitis etc

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Αρχειοθήκη ιστολογίου

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Η λίστα ιστολογίων μου

Κυριακή 21 Φεβρουαρίου 2016

Abstract

Abstract

Background

Objective

Methods

Results

Conclusion

Abstract

Abstract

Αρχειοθήκη ιστολογίου