Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

Αρχειοθήκη ιστολογίου

! # Ola via Alexandros G.Sfakianakis on Inoreader

Η λίστα ιστολογίων μου

Παρασκευή 30 Μαρτίου 2018

Instrument detection and pose estimation with rigid part mixtures model in video-assisted surgeries

Publication date: Available online 30 March 2018
Source:Medical Image Analysis
Author(s): Daniel Wesierski, Anna Jezierska
Localizing instrument parts in video-assisted surgeries is an attractive and open computer vision problem. A working algorithm would immediately find applications in computer-aided interventions in the operating theater. Knowing the location of tool parts could help virtually augment visual faculty of surgeons, assess skills of novice surgeons, and increase autonomy of surgical robots. A surgical tool varies in appearance due to articulation, viewpoint changes, and noise. We introduce a new method for detection and pose estimation of multiple non-rigid and robotic tools in surgical videos. The method uses a rigidly structured, bipartite model of end-effector and shaft parts that consistently encode diverse, pose-specific appearance mixtures of the tool. This rigid part mixtures model then jointly explains the evolving tool structure by switching between mixture components. Rigidly capturing end-effector appearance allows explicit transfer of keypoint meta-data of the detected components for full 2D pose estimation. The detector can as well delineate precise skeleton of the end-effector by transferring additional keypoints. To this end, we propose effective procedure for learning such rigid mixtures from videos and for pooling the modeled shaft part that undergoes frequent truncation at the border of the imaged scene. Notably, extensive diagnostic experiments inform that feature regularization is a key to fine-tune the model in the presence of inherent appearance bias in videos. Experiments further illustrate that estimation of end-effector pose improves upon including the shaft part in the model. We then evaluate our approach on publicly available datasets of in-vivo sequences of non-rigid tools and demonstrate state-of-the-art results.

Graphical abstract

image


https://ift.tt/2GqoY5I

Deep Embedding Convolutional Neural Network for Synthesizing CT Image from T1-Weighted MR Image

Publication date: Available online 30 March 2018
Source:Medical Image Analysis
Author(s): Lei Xiang, Qian Wang, Dong Nie, Lichi Zhang, Xiyao Jin, Yu Qiao, Dinggang Shen
Recently, more and more attention is drawn to the field of medical image synthesis across modalities. Among them, the synthesis of computed tomography (CT) image from T1-weighted magnetic resonance (MR) image is of great importance, although the mapping between them is highly complex due to large gaps of appearances of the two modalities. In this work, we aim to tackle this MR-to-CT synthesis task by a novel deep embedding convolutional neural network (DECNN). Specifically, we generate the feature maps from MR images, and then transform these feature maps forward through convolutional layers in the network. We can further compute a tentative CT synthesis from the midway of the flow of feature maps, and then embed this tentative CT synthesis result back to the feature maps. This embedding operation results in better feature maps, which are further transformed forward in DECNN. After repeating this embedding procedure for several times in the network, we can eventually synthesize a final CT image in the end of the DECNN. We have validated our proposed method on both brain and prostate imaging datasets, by also comparing with the state-of-the-art methods. Experimental results suggest that our DECNN (with repeated embedding operations) demonstrates its superior performances, in terms of both the perceptive quality of the synthesized CT image and the run-time cost for synthesizing a CT image.



https://ift.tt/2pTq2Jf

Datasets for correlation dynamics of cocoa production in South Western Nigeria

elsevier-non-solus.png

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): S.O. Edeki, M.E. Adeosun, G.O. Akinlabi, O.M. Ofuyatan
In the Nigeria economy, cocoa production has been of great importance. This buttresses the fact that cocoa as a product is the leading agricultural export of Nigeria, leaving the country currently as the world fourth largest producer of cocoa, after Ivory Coast, Indonesia and Ghana and the third largest exporter, after Ivory Coast and Ghana. Hence, there is need for the agricultural sector expansion, effective predictive models and reliable price mechanism. This article examines tonnes of cocoa production dataset of the Nigeria agricultural sector for the period of twenty-four (24) years spanning between 1993 to 2016. The Correlation dynamics examined includes the autocorrelation features as affected by the production rate within the considered time interval. The degree of similarity between the dataset and the corresponding lagged version of itself over successive time interval is measured using a serial correlation test while the results mostly favour negative correlation showing that large current values correspond to small values at the specified lag. These dataset can effectively serve as good candidate for agricultural product modelling in terms of forecasting.



https://ift.tt/2E7fLxh

Taiwanese consumer survey data for investigating the role of information on equivalence of organic standards in directing food choice

elsevier-non-solus.png

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Ching-Hua Yeh, Monika Hartmann, Stefan Hirsch
The presentation of credence attributes such as the product's origin or the production method has a significant influence on consumers' food purchase decisions. The dataset includes survey responses from a discrete choice experiment with 1309 food shoppers in Taiwan using the example of sweet pepper. The survey was carried out in 2014 in the three largest Taiwanese cities. It evaluates the impact of providing information on the equality of organic standards on consumers' preferences at the example of sweet pepper. Equality of organic standards implies that regardless of products' country-of-origin (COO) organic certifications are based on the same production regulation and managerial processes. Respondents were randomly allocated to the information treatment and the control group. The dataset contains the product choices of participants in both groups, as well as their sociodemographic information.



https://ift.tt/2pU4bAr

Data on drying kinetics of a semi-automated gas-fired fish dryer

elsevier-non-solus.png

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Idehai O. Ohijeagbon, Olumuyiwa A. Lasode, Segun Adebayo, Oluseyi O. Ajayi, Olugbenga A. Omotosho
The dataset presented in this article represent the drying characteristics of a semi-automated gas-fired fish dryer. A cabinet dryer was constructed mainly from mild steel sheet metal and stainless steel, and was used for drying prepared fish samples of Clarias gariepinus species. Major operating parameters which included mass of fish, mass of Liquefied Petroleum Gas used, inside temperature of the dryer, and drying time were monitored. Also, output parameters such as the moisture content and energy utilization amongst others were observed and recorded at varying time intervals and specified drying temperatures. The drying temperature was sustained via an incorporated PID temperature controller that allowed drying to proceed until a relatively constant mass of the dried fish samples was attained. The information contained in this data article include a schematic drawing of drying kinetics analysis of the semi-automated gas-fired fish dryer and a pictorial view of the gas-fired cabinet dryer. Also included are pictorial representations of the washed and neatly folded degutted fish samples and dried fish samples. Data provided in this article are those relating to process parameters of the semi-automated fish dryer, data of output parameters of the fish dryer and comparisons of moisture content and energy utilization at different drying temperatures with time.



https://ift.tt/2J5sB2R

Transoral endoscopic thyroid surgery via the tri‐vestibular approach with a hybrid space‐maintaining method: A preliminary report

Head &Neck, EarlyView.


https://ift.tt/2Go3JBt

Systematic review evaluating randomized controlled trials of smoking and alcohol cessation interventions in people with head and neck cancer and oral dysplasia

Head &Neck, EarlyView.


https://ift.tt/2GDXSLT

Case Report of an open Neck Procedure Complication Associated with Laryngeal Mask Airway Use.

Related Articles

Case Report of an open Neck Procedure Complication Associated with Laryngeal Mask Airway Use.

Iran J Otorhinolaryngol. 2018 Mar;30(97):121-123

Authors: Subramaniam T, Casserly P

Abstract
Introduction: The laryngeal mask airway (LMA) is a safe method to establish airway control during general anaesthesia. In otolaryngology surgery, the use of a LMA is well established in ear surgery; however, the use of a LMA during open neck procedures remains controversial. We report a case in which the pharyngeal distortion by the LMA cuff resulted in an iatrogenic complication.
Case Report: A 38-year-old female with a background of multiple myeloma was referred to the otolaryngology team for an open cervical lymph node biopsy. The patient was in remission after a 2 year post chemotherapy treatment, but now presented with a 4-week history of persistent nodal enlargement. During the elective procedure, pharyngeal distortion from the laryngeal airway mask used for airway management resulted in an iatrogenic pharyngeal injury. This case is reported to highlight the importance of communication between the surgeon and anesthetist about the mode of airway management in open neck surgery.
Conclusion: Communication between the otolaryngologist and anesthetist is pertinent when selecting the method of airway management in open neck procedures. A LMA should be used with caution during open neck procedures, with the surgeon recognizing the potential for pharyngeal distortion.

PMID: 29594081 [PubMed]



https://ift.tt/2uzeOy2

Surgery and Anesthesia Management for Intraoral Synechia: A Case Report.

Related Articles

Surgery and Anesthesia Management for Intraoral Synechia: A Case Report.

Iran J Otorhinolaryngol. 2018 Mar;30(97):117-119

Authors: Gharavifard M, Kashani I, Joudi M, Sharifian M, Sayedi SJ, Mohammadipanah B, Jamali-Behnam F

Abstract
Introduction: Intraoral synechia is a rare congenital condition, generally associated with other maxillo-facial malformations. We present a neonate with congenital intraoral bilateral synechia without any other facial anomalies.
Case Report: In this paper, we present a 19-day-old male neonate with congenital intraoral bilateral synechia without any other facial anomalies. We review the literature to discuss the surgical and anesthesia management of this rare congenital disease.
Conclusion: The disease manifested with a wide spectrum of symptoms. Most cases need surgery and airway management. In patients with a low risk of bleeding or a compromised airway, it is possible to manage them with face mask-inhalation anesthesia and maintain spontaneous breathing.

PMID: 29594080 [PubMed]



https://ift.tt/2GmDj7p

Sudden Bilateral Sensorineural Hearing Loss Secondary to Cerebral Venous Thrombosis.

Related Articles

Sudden Bilateral Sensorineural Hearing Loss Secondary to Cerebral Venous Thrombosis.

Iran J Otorhinolaryngol. 2018 Mar;30(97):113-116

Authors: Ishak MN, Nik-Abdul-Ghani NM, Mohamad I

Abstract
Introduction: Sudden sensorineural hearing loss (SSNHL) is an important otological emergency. Up to 90% of the cases are idiopathic. Cerebral venous thrombosis (CVT) is an extremely rare identifiable cause as it only represents 0.5% of all strokes.
Case Report: In this paper, an unusual case of bilateral SSNHL secondary to bilateral CVT with rapid and complete recovery is reported. The patient presented with sudden bilateral hearing loss associated with some neurological symptoms. Initial computed tomography (CT) venography revealed a CVT of bilateral transverse sinuses. The patient was started on an anticoagulant and imaging was repeated after five days, revealing the absence of the thrombosis. Serial pure tone audiometry (PTA) showed complete recovery of bilateral hearing within 10 days.
Conclusion: Early detection and intervention may fasten hearing recovery and improve the quality of life. The immediate restoration of venous blood flow and intracranial pressure may lead to the complete recovery of bilateral hearing loss.

PMID: 29594079 [PubMed]



https://ift.tt/2IgaUw4

Malignant Melanoma of the External Auditory Canal: A Rare Entity.

Related Articles

Malignant Melanoma of the External Auditory Canal: A Rare Entity.

Iran J Otorhinolaryngol. 2018 Mar;30(97):107-111

Authors: Khonglah Y, Das N, Raphael V, Jitani AK, Shunyu NB

Abstract
Introduction: Although malignant melanomas (MM) are common in the head and neck region; primary malignant melanoma of the external auditory canal (EAC) is rare.
Case Report: We present the case of a 50-year-old symptomatic man with a malignant melanoma of the external auditory canal, which clinically masqueraded as a haemangioma. The patient subsequently developed extensive loco-regional metastasis, requiring extensive surgery. We describe the clinical presentation, differential diagnosis, both clinical and pathological in terms of other pigmented lesions in the external auditory canal, detailed histopathology, and literature review.
Conclusion: We want to emphasize the importance of immediate and adequate biopsy of any pigmented lesion in the head and neck region to rule out MM. Also, we emphasize the importance of deep biopsy for proper histopathological assessment in addition to distinguishing it from benign melanocytic nevi, in order to initiate treatment.

PMID: 29594078 [PubMed]



https://ift.tt/2IfhzGL

Results of Type I Tympanoplasty Using Fascia with or without Cartilage Reinforcement: 10 Years' Experience.

Related Articles

Results of Type I Tympanoplasty Using Fascia with or without Cartilage Reinforcement: 10 Years' Experience.

Iran J Otorhinolaryngol. 2018 Mar;30(97):103-106

Authors: Kouhi A, Khorsandi Ashthiani MT, Jalali MM

Abstract
Introduction: There remains controversy about the optimal kind of graft to repair tympanic membrane. The purpose of this study was to evaluate the anatomical and auditory outcomes of type I tympanoplasty using fascia with or without cartilage reinforcement.
Materials and Methods: This retrospective cohort study was conducted from 2005 to 2015. All cases were surgically treated by a single surgeon. We excluded cases in which the etiology of chronic otitis media was cholesteatoma. According to the use of cartilage reinforcement in the posterosuperior part of the graft, patients were divided into two groups, and the results of anatomical and auditory evaluation were compared between the two groups. The anatomical outcome was grafting success and the auditory outcome was improvement of air bone gap (ABG).
Results: A total of 320 patients were classified in Group A (tympanoplasty with fascia temporalis only) and 346 were in Group B (tympanoplasty with cartilage reinforcement). All patients were followed for at least 2 years. The overall success rate in the two groups was 91.6% and 93.4%, respectively (P=0.3). The most common cause of failure in the two groups was re-perforation (5.6% and 3.8%, respectively). The improvement of ABG in two groups was 18.5 dB and 3.2 dB, respectively. The difference between two groups was statistically significant (P<0.001).
Conclusion: In patients with dry perforation of the tympanic membrane, the anatomical success with tympanoplasty with fascia only or with cartilage reinforcement was similar. However, hearing improvement in the fascia only group was greater than in the group undergoing cartilage reinforcement.

PMID: 29594077 [PubMed]



https://ift.tt/2J5ylt6

Translation, Cross-Cultural Adaptation, Validation and Reliability of the Northwestern Dysphagia Patient Check Sheet (NDPCS) in Iran.

Related Articles

Translation, Cross-Cultural Adaptation, Validation and Reliability of the Northwestern Dysphagia Patient Check Sheet (NDPCS) in Iran.

Iran J Otorhinolaryngol. 2018 Mar;30(97):97-101

Authors: Bakhtiyari J, Salmani M, Noruzi R, Sarraf P, Barzegar E, Mirmohammadkhani M

Abstract
Introduction: Speech and language therapists (SLTs) require proper tools to detect dysphagia in the early stages. One of these screening tools is the Northwestern Dysphagia Patient Check Sheet (NDPCS). However, this tool needs to be adapted, validated, and shown to be reliable for the Persian culture. The aim of the present study was to report the validity and reliability of the Persian NDPCS (P-NDPCS).
Materials and Methods: The NDPCS has 28 items and five sections. Beaton's guidelines were followed in terms of the translation process. To report the content validity index (CVI) and the content validity ratio (CVR), eight SLTs experienced in swallowing disorders examined the content and face validities of the P-NDPCS in terms of the quality of translation, fluency, understandability, and the cultural context. In total, 140 patients with neurogenic and mechanical dysphagia were evaluated using the P-NDPCS. Internal consistency reliability was investigated using the Kuder-Richardson formula 20. The interclass correlation coefficient (ICC) was used for test-retest reliability.
Results: The P-NDPCS preserved the 28 items and the five categories of the original version. However, semantic and food adjustments were applied due to cultural differences. The scoring system was changed from safe/unsafe to yes/no for four subsections and to normal/abnormal for the oromotor section. Food requirements were also changed. The CVR and CVI were both 75%. The P-NDPCS was shown to have good content validity. The internal reliability was 0.95, indicating excellent reliability.
Conclusion: The equivalence between the original version of the NDPCS and the P-NDPCS was preserved. Our findings indicate that the P-NDPCSis a valid and reliable screening tool for the diagnosis of dysphagia in the early phase.

PMID: 29594076 [PubMed]



https://ift.tt/2GKqbrM

Islamic Attitudes and Rhinoplasty.

Related Articles

Islamic Attitudes and Rhinoplasty.

Iran J Otorhinolaryngol. 2018 Mar;30(97):91-96

Authors: Bakhshaee M, Asghari M, Sharifian MR, Jafari Ashtiyani S, Rasoulian B

Abstract
Introduction: Although the psychological aspects of rhinoplasty have been fully investigated in the medical literature, the religiosity of rhinoplasty candidates has not been taken into consideration.
Materials and Methods: In this cross-sectional study, the religious attitudes of 157 rhinoplasty candidates were compared with those of 74 subjects who had not requested rhinoplasty. A domestic validated reliable questionnaire was completed by all subjects to classify them with respect to religious attitude. Other factors such as age, sex and economic and educational status were also taken into consideration. From the surgeon's perspective, subjects were put into three categories: subjects who had a relative indication for rhinoplasty (Category.1), subjects with a well-defined nose based on accepted standards of facial aesthetic analysis (Category.2) and finally subjects with a severely deformed nose, such as deviated nose or nasal cleft lip deformity (Category.3).
Results: The mean age among subjects was 28.63 ± 7.05 years, and the majority were female (87%). The two groups of participants (those who did and did not express a desire for rhinoplasty) were analyzed from the point view of age, sex, economic and educational status. The economic and educational status of the two groups did not differ significantly (P>0.05). The religious score showed a significant difference between those who were interested in rhinoplasty (122.75±23.49) and those were not interested (138.78±21.85; P<0.001).
Conclusion: Religion may affect a patient's decision to undergo rhinoplasty surgery, such that persons with a higher religious attitude tend to undertake it less often. However, individuals with major nasal deformities tend to decide undertake the surgery, irrespective of religious beliefs.

PMID: 29594075 [PubMed]



https://ift.tt/2pRty6V

Tonsillar Lymphoma in Children According to Age Group: A Systematic Review.

Related Articles

Tonsillar Lymphoma in Children According to Age Group: A Systematic Review.

Iran J Otorhinolaryngol. 2018 Mar;30(97):69-75

Authors: de Carvalho GM, Pauna HF, Crespo AN, Gusmão RJ, Guimarães AC

Abstract
Introduction: Lymphoma is a common malignant tumor of the head and neck occurring during childhood. Early diagnosis is very important in terms of prognosis in patients with tonsillar lymphoma.Our objective was to evaluate the clinical manifestations of pediatric tonsillar lymphoma according to different age groups.
Materials and Methods: A systematic review of available English, Spanish, or Portuguese literature from January 1996 to June 2012 was performedin the BIREME, Cochrane, IBECS, Lilacs, PubMed/Medline, SCIELO, and Scopus databases, using "tonsillar lymphoma" and "children" as keywords. Inclusion criteria were pediatric case reports, patients aged up to 18 years, and information on clinical features at the time of diagnosis.
Results: Out of 87 identified publications, 13 articles were selected describing 53 patients. Tonsillar asymmetry was the most common sign. Snoring is a common sign in patients aged under 5 years; clinical lymphadenopathy is frequent among patients aged between 6 and 10 years; and dysphagia is a common sign in patients between 11 and 18 years of age. Burkitt's lymphoma is the most common form among all ages studied, followed by B-cell lymphoma.
Conclusion: Clinical manifestations differ according to age group. However, tonsillar asymmetry is the most frequent sign regardless of age group.

PMID: 29594072 [PubMed]



https://ift.tt/2GXS11o

Hourly pattern of allergenic alder and birch pollen concentrations in the air: Spatial differentiation and the effect of meteorological conditions

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): K. Borycka, I. Kasprzyk
In temperate climate widespread alder and birch are an important source of strong aeroallergens. The diurnal pattern of airborne pollen concentrations depends on the rhythm of pollen release from anthers as well as weather conditions, convection air currents, long-distance transport, pollen trap location and local vegetation. The aim of the study was to present a diurnal pattern of Alnus (alder) and Betula (birch) pollen concentrations in the air in a horizontal and vertical gradient and examine the weather parameters that had the greatest impact on the pattern. The study was conducted in Rzeszów City, southeast Poland over three years. Pollen grains were collected using a Hirst volumetric spore trap at three sampling points: two at 12 m the agl, and one at 1.5 m agl. Data were analysed using circular statistics and a nonlinear function. For alder, three models of hourly patterns were elaborated and the most common presented early morning minimum and early afternoon maximum. For birch, the most common model has one peak at night and a marked decrease in concentrations in early morning, although a second model has peak during early afternoon. A model with 3 peaks is much less common. These models are characteristic for warm temperate climate regions, where alders and birches are common. The diurnal patterns did not depend on the localization of traps or proximity of the pollen source, although these factors influenced the hourly concentrations, with higher values observed at roof level. Significant relationships between the hourly pollen counts and meteorological parameters were observed only for alder. Three incidents of increasing birch pollen concentrations were observed during the first two hours of precipitation and linked to a convection effect. Unstable weather conditions caused by air convection might strongly modify the circadian pattern and cause the nightly peaks concentrations. The general results are that people suffering from allergies may be exposed to high birch and alder pollen concentrations at almost all times during the day with the exception of the morning hours, as well as during the first hours of convective precipitation. Precise information on pollen concentrations aids in allergy prevention, so cities should provide aerobiological monitoring at least at "roof level" and "nose level".

Graphical abstract

image


https://ift.tt/2GY1N3L

Air quality and human health impacts of grasslands and shrublands in the United States

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Varsha Gopalakrishnan, Satoshi Hirabayashi, Guy Ziv, Bhavik R. Bakshi
Vegetation including canopy, grasslands, and shrublands can directly sequester pollutants onto the plant surface, resulting in an improvement in air quality. Until now, several studies have estimated the pollution removal capacity of canopy cover at the level of a county, but no such work exists for grasslands and shrublands. This work quantifies the air pollution removal capacity of grasslands and shrublands at the county-level in the United States and estimates the human health benefits associated with pollution removal using the i-Tree Eco model. Sequestration of pollutants is estimated based on the Leaf Area Index (LAI) obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) derived dataset estimates of LAI and the percentage land cover obtained from the National Land Cover Database (NLCD) for the year 2010. Calculation of pollution removal capacity using local environmental data indicates that grasslands and shrublands remove a total of 6.42 million tonnes of air pollutants in the United States and the associated monetary benefits total $268 million. Human health impacts and associated monetary value due to pollution removal was observed to be significantly high in urban areas indicating that grasslands and shrublands are equally critical as canopy in improving air quality and human health in urban regions.

Graphical abstract

image


https://ift.tt/2pUuaYz

Experimental investigation of tread wear and particle emission from tyres with different treadwear marking

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Theodoros Grigoratos, Mats Gustafsson, Olle Eriksson, Giorgio Martini
The Treadwear Rating (TWR) provided on the sidewall of the tyre is a marking intended to inform the customer about the expected durability of the tyre. The current study explores whether there is a correlation between the TWR and tyres' tread mass loss. Furthermore, it explores the possible correlation between the TWR and tyre wear dust emitted in the form of PM10 and PM2.5. For that reason, two tyres of the same brand (B) but with different TWR and three tyres of different brands (C and D with the same TWR as one of the B tyres and A with a lower TWR) were tested at a constant speed of 70 km/h by means of the Swedish National Road and Transport Research Institute (VTI) road simulator. Tyres of the same TWR but of different brands showed different behaviour in terms of material loss, PM, and PN emissions under the selected testing conditions. This means that it is not feasible to categorize tyres of different brands in terms of their emissions based on their TWR. The test performed on the two tyres of the same brand but with different TWR showed instead a substantial (not statistically significant) difference in both total wear and PM10 emissions. The tyre with the higher TWR (B2) showed less wear and PM10 emissions compared to the B1 tyre having a lower TWR. Since only two tyres of the same brand and with different TWR were tested, this result cannot be generalized and more tests are necessary to confirm the relation within the same brand. In general, the tyre tread mass loss showed no obvious statistical relation to PM10, PM2.5 or PN concentration. In all cases approximately 50% (by mass) of emitted PM10 fall within the size range of fine particles, while PN size distribution is dominated by nanoparticles most often peaking at 20–30 nm.



https://ift.tt/2GX7RcE

Operational evaluation of the RLINE dispersion model for studies of traffic-related air pollutants

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Chad W. Milando, Stuart A. Batterman
Exposure to traffic-related air pollutants (TRAP) remains a key public health issue, and improved exposure measures are needed to support health impact and epidemiologic studies and inform regulatory responses. The recently developed Research LINE source model (RLINE), a Gaussian line source dispersion model, has been used in several epidemiologic studies of TRAP exposure, but evaluations of RLINE's performance in such applications have been limited. This study provides an operational evaluation of RLINE in which predictions of NOx, CO and PM2.5 are compared to observations at air quality monitoring stations located near high traffic roads in Detroit, MI. For CO and NOx, model performance was best at sites close to major roads, during downwind conditions, during weekdays, and during certain seasons. For PM2.5, the ability to discern local and particularly the traffic-related portion was limited, a result of high background levels, the sparseness of the monitoring network, and large uncertainties for certain processes (e.g., formation of secondary aerosols) and non-mobile sources (e.g., area, fugitive). Overall, RLINE's performance in near-road environments suggests its usefulness for estimating spatially- and temporally-resolved exposures. The study highlights considerations relevant to health impact and epidemiologic applications, including the importance of selecting appropriate pollutants, using appropriate monitoring approaches, considering prevailing wind directions during study design, and accounting for uncertainty.



https://ift.tt/2E8UoeO

Temporal and spatial characteristics of dust devils and their contribution to the aerosol budget in East Asia—An analysis using a new parameterization scheme for dust devils

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Yaoguo Tang, Yongxiang Han, Zhaohuan Liu
Dust aerosols are the main aerosol components of the atmosphere that affect climate change, but the contribution of dust devils to the atmospheric dust aerosol budget is uncertain. In this study, a new parameterization scheme for dust devils was established and coupled with WRF-Chem, and the diurnal and monthly variations and the contribution of dust devils to the atmospheric dust aerosol budget in East Asia was simulated. The results show that 1) both the diurnal and monthly variations in dust devil emissions in East Asia had unimodal distributions, with peaks in the afternoon and the summer that were similar to the observations; 2) the simulated dust devils occurred frequently in deserts, including the Gobi. The distributed area and the intensity center of the dust devil moved from east to west during the day; 3) the ratio between the availability of convective buoyancy relative to the frictional dissipation was the main factor that limited the presence of dust devils. The position of the dust devil formation, the surface temperature, and the boundary layer height determined the dust devil intensity; 4) the contribution of dust devils to atmospheric dust aerosols determined in East Asia was 30.4 ± 13%, thereby suggesting that dust devils contribute significantly to the total amount of atmospheric dust aerosols. Although the new parameterization scheme for dust devils was rough, it was helpful for understanding the distribution of dust devils and their contribution to the dust aerosol budget.

Graphical abstract

image


https://ift.tt/2GX7LBO

VOCs emission characteristics and priority control analysis based on VOCs emission inventories and ozone formation potentials in Zhoushan

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Qiaoli Wang, Sujing Li, Minli Dong, Wei Li, Xiang Gao, Rongmin Ye, Dongxiao Zhang
Zhoushan is an island city with booming tourism and service industry, but also has many developed VOCs and/or NOX emission industries. It is necessary to carry out regional VOCs and O3 pollution control in Zhoushan as the only new area owns the provincial economic and social administration rights. Anthropogenic VOCs emission inventories were built based on emission factor method and main emission sources were identified according to the emission inventories. Then, localized VOCs source profiles were built based on in-site sampling and referring to other studies. Furthermore, ozone formation potentials (OFPs) profiles were built through VOCs source profiles and maximum incremental reactivity (MIR) theory. At last, the priority control analysis results showed that industrial processes, especially surface coating, are the key of VOCs and O3 control. Alkanes were the most emitted group, accounting for 58.67%, while aromatics contributed the most to ozone production accounting for 69.97% in total OFPs. n-butane, m/p-xylene, i-pentane, n-decane, toluene, propane, n-undecane, o-xylene, methyl cyclohexane and ethyl benzene were the top 10 VOC species that should be preferentially controlled for VOCs emission control. However, m/p-xylene, o-xylene, ethylene, n-butane, toluene, propene, 1,2,4-trimethyl benzene, 1,3,5-trimethyl benzene, ethyl benzene and 1,2,3-trimethyl benzene were the top 10 VOC species that required preferential control for O3 pollution control.



https://ift.tt/2E87oBE

A new methodology to derive settleable particulate matter guidelines to assist policy-makers on reducing public nuisance

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Milena Machado, Jane Meri Santos, Valdério Anselmo Reisen, Neyval Costa Reis, Ilias Mavroidis, Ana T. Lima
Air quality standards for settleable particulate matter (SPM) are found in many countries around the world. As well known, annoyance caused by SPM can be considered a community problem even if only a small proportion of the population is bothered at rather infrequent occasions. Many authors have shown that SPM cause soiling in residential and urban environments and degradation of materials (eg, objects and surface painting) that can impair the use and enjoyment of property and alter the normal activities of society. In this context, this paper has as main contribution to propose a guidance to establish air quality standards for annoyance caused by SPM in metropolitan industrial areas. To attain this objective, a new methodology is proposed which is based on the nonlinear correlation between the perceived annoyance (qualitative variable) and particles deposition rate (quantitative variable). Since the response variable is binary (annoyed and not annoyed), the logistic regression model is used to estimate the probability of people being annoyed at different levels of particles deposition rate and to compute the odds ratio function which gives, under a specific level of particles deposition rate, the estimated expected value of the population perceived annoyance. The proposed methodology is verified in a data set measured in the metropolitan area of Great Vitória, Espirito Santo, Brazil. As a general conclusion, the estimated probability function of perceived annoyance as a function of SPM has shown that 17% of inhabitants report annoyance to very low particles deposition levels of 5 g/(m2∙30 days). In addition, for an increasing of 1 g/(m2∙30 days) of SPM, the smallest estimated odds ratio of perceived annoyance by a factor of 1.5, implying that the probability of occurrence is almost 2 times as large as the probability of no occurrence of annoyance.

Graphical abstract

image


https://ift.tt/2H0ReNy

Diurnal spatial distributions of aerosol optical and cloud micro-macrophysics properties in Africa based on MODIS observations

S13522310.gif

Publication date: June 2018
Source:Atmospheric Environment, Volume 182
Author(s): Didier Ntwali, Hongbin Chen
The diurnal spatial distribution of both natural and anthropogenic aerosols, as well as liquid and ice cloud micro-macrophysics have been evaluated over Africa using Terra and Aqua MODIS collection 6 products. The variability of aerosol optical depth (AOD), Ångström exponent (AE), liquid and ice cloud microphysics (Liquid cloud effective radius LCER, Ice cloud effective radius ICER) and cloud macrophysics (Liquid cloud optical thickness LCOT, Liquid cloud water path LCWP, Ice cloud optical thickness ICOT, Ice cloud water path ICWP) parameters were investigated from the morning to afternoon over Africa from 2010 to 2014. In both the morning (Terra) and afternoon (Aqua) heavy pollution (AOD ≥ 0.6) occurs in the coastal and central areas (between 120 N-170 N and 100 E-150 E) of West of Africa (WA), Central of Africa (CA) (0.50 S-70S and 100 E-250 E),. Moderate pollution (0.3 < AOD < 0.6) often occurs in West and North of Africa (between 50 N-270 N and 160 W-50E), and clean environmental (AOD < 0.3) conditions are common in South of Africa (SA), East of Africa (EA) and some regions in North of Africa (NA). The West-North of Africa (WNA) and Central-South of Africa (CSA) regions are dominated by dust (AE < 0.7) and biomass burning (AE > 1.2) aerosols. The mixture of dust and biomass burning aerosols (0.7 < AE < 1.2) are found at the coastal areas in West of Africa (CoWA) and Central of Africa (CA) (50 N-80N and 100 E-340 E), particularly in the morning and afternoon respectively. The LCER often decrease from the morning to the afternoon in all seasons, but an increase occur from the morning to the afternoon in CSA (50 S-220 S) in DJF, both CA (20 S-50N) and CoWA in JJA and SON. The ICER increase from the morning to afternoon in all seasons over Africa and decreases in South of Africa (50 S-200 S) in DJF. The LCOT increases from the morning to afternoon in NA and SA while a decrease occur in CA in all seasons. The LCWP increase in many regions of Africa in all seasons while a decrease occurs in CoWA during JJA. The ICOT and ICWP show a remarkable increase from the morning to afternoon in regions dominated by biomass burning (CSA) compared to regions dominated by dust (WNA) aerosols in DJF, MAM and SON. Dust aerosols are mainly distributed in WNA by northerly and westerly winds in both January and April, southerly and southwesterly winds in July, and southerly and southwesterly winds in October, while biomass burning aerosols are mainly distributed in CSA by the northerly and northeasterly winds in January, easterly winds in April, July and October. The diurnal variability of cloud parameters is associated with both convective processes and cloud types. The knowledge of interactions between natural and anthropogenic aerosols with liquid and ice cloud microphysics parameters could contribute to improve aerosol and cloud remote sensing retrieval.



https://ift.tt/2E795PJ

Three-dimensional cluster formation and structure in heterogeneous dose distribution of intensity modulated radiation therapy

S01678140.gif

Publication date: Available online 30 March 2018
Source:Radiotherapy and Oncology
Author(s): Ming Chao, Jie Wei, Ganesh Narayanasamy, Yading Yuan, Yeh-Chi Lo, José A. Peñagarícano
PurposeTo investigate three-dimensional cluster structure and its correlation to clinical endpoint in heterogeneous dose distributions from intensity modulated radiation therapy.MethodsTwenty-five clinical plans from twenty-one head and neck (HN) patients were used for a phenomenological study of the cluster structure formed from the dose distributions of organs at risks (OARs) close to the planning target volumes (PTVs). Initially, OAR clusters were searched to examine the pattern consistence among ten HN patients and five clinically similar plans from another HN patient. Second, clusters of the esophagus from another ten HN patients were scrutinized to correlate their sizes to radiobiological parameters. Finally, an extensive Monte Carlo (MC) procedure was implemented to gain deeper insights into the behavioral properties of the cluster formation.ResultsClinical studies showed that OAR clusters had drastic differences despite similar PTV coverage among different patients, and the radiobiological parameters failed to positively correlate with the cluster sizes. MC study demonstrated the inverse relationship between the cluster size and the cluster connectivity, and the nonlinear changes in cluster size with dose thresholds. In addition, the clusters were insensitive to the shape of OARs.ConclusionThe results demonstrated that the cluster size could serve as an insightful index of normal tissue damage. The clinical outcome of the same dose–volume might be potentially different.



https://ift.tt/2J332PT

Application of a webcam camera as a cost-effective sensor with image processing for dual electrochemical – colorimetric detection system

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Kajorngai Thajee, Pathinan Paengnakorn, Wasin Wongwilai, Kate Grudpan
A webcam camera as an alternative cost-effective colorimetric sensor for the simple dual electrochemical – colorimetric detection system is proposed. Performance test of the system was investigated through electrochemical and colorimetric behaviors of redox reactions of potassium ferrocyanide complex. Image processing with two color systems, RGB and HSV, was applied together with various electrochemical techniques. Color responses obtained from the webcam correlated with electrochemical signals in various electrochemical techniques. The results from the proposed dual detection system agreed well with those obtained using conventional UV–vis spectroelectrochemical system reported previously. The application of proposed system was demonstrated for studying the redox reaction of catechin and electrochemical - colorimetric behaviors of Fe(II) and Fe(III) mixture.

Graphical abstract

image


https://ift.tt/2E76e9t

A fast sample processing strategy for large-scale profiling of human urine phosphoproteome by mass spectrometry

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Xinyuan Zhao, Wanjun Zhang, Tong Liu, Hangyan Dong, Junjie Huang, Changqing Sun, Guangshun Wang, Xiaohong Qian, Weijie Qin
Liquid biopsies using body fluids have gained much attention in recent years due to their multiple advantages in clinical diagnosis, such as less/non-invasive collection, suitability for longitudinal disease monitoring, and better representation of tumor heterogeneity. As an attractive choice for liquid biopsy, urine proteins and their post-translational modifications (PTMs) have the potential to offer significant insights into physiological variations and pathological changes in the human body. However, due to the intrinsically large variability of urine proteins and their PTMs among different individuals, there is a high demand for strategies for high-throughput analysis of a large number of samples to obtain a comprehensive view and a reliable reference interval of the urine proteome. In this work, we proposed a new urine phosphoproteome sample processing strategy that combines fast protein extraction, efficient multiple immobilized-proteases digestion, and tandemly connected centrifugal tips device-based facile phosphopeptide enrichment & fractionation. This strategy is capable of paralleled sample processing with an approximate five-fold reduction in processing time and is therefore particularly suitable for handling a large number of urine samples. Totally, we identified 4196 phosphosites in human urine proteins by mass spectrometry in replicated tests, a number which is dozens of times larger than those previously reported. Therefore, this strategy may have great potential in urine-based phosphoprotein biomarker screening and drug response studies.

Graphical abstract

image


https://ift.tt/2GYkHrc

Affinity capture surface carboxyl-functionalized MoS2 sheets to enhance the sensitivity of surface plasmon resonance immunosensors

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Nan-Fu Chiu, Ting-Li Lin
The development of functionalized molybdenum disulfide (MoS2) has led to a new trend in the biosensing field, owing to its high sensitivity and bio-affinity characteristics with regards to the simple synthesis of carboxyl-functionalized MoS2 nanocomposites. In this study, we used monochloroacetic acid (MCA) to successfully modify carboxyl-MoS2. The efficiency of this MCA modification method showed a higher -COOH group content of 30.1%, mainly due to chlorine atoms occupying the MoS2 sulfur vacancy to allow for the formation of a strong bonding effect. This then enhanced the surface area of -COOH and improved the formation of covalent bonds between proteins. We demonstrated that MoS2-COOH-based surface plasmon resonance (SPR) chips can provide excellent sensitivity and high affinity for immunoassay biomolecules detected in a low sample volume of 20 μl. With respect to the shifts of the SPR angles of the chips, the high binding affinity at a BSA concentration of 14.5 nM for a MoS2-COOH chip, a MoS2 chip and a traditional SPR chip are 4.69 m°, 2.49 m° and 1.53 m°, respectively. In addition, the MoS2-COOH chip could amplify the SPR angle response by 3.1 folds and enhance the high association rate of ka by 212 folds compared to MoS2 and traditional SPR chips. The results thus obtained revealed that the overall affinity binding value, KA, of the MoS2-COOH chip can be significantly enhanced by up to ∼ 6.5 folds that of the MoS2 chip. In summary, the excellent binding affinity, biocompatible and high sensitivity suggest the potential of the clinical application of this MoS2-COOH-based SPR chip detection method for in vitro diagnostic and point-of-care testing devices.

Graphical abstract

image


https://ift.tt/2pTLe0E

Synthesis of penetrable poly(methacrylic acid-co-ethylene glycol dimethacrylate) microsphere and its HPLC application in protein separation

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Xiao Sun, Jing Li, Li Xu
In the present study, the narrow-dispersed penetrable poly(methacrylic acid-co-ethylene glycol dimethacrylate) (poly(MAA-co-EDMA)) microspheres were successfully synthesized based on the sacrificial support method. The poly(MAA-co-EDMA) microspheres mirrored the porous structure of the sacrificial support, i.e. penetrable silica, characteristic of copious mesopores and throughpores. In addition, they possessed large surface area, adjustable hydrophobicity and the cation-exchange ability. Owing to their multi functionalities, they were applied as chromatographic stationary phase to separate proteins in different separation modes, including reversed phase, hydrophobic interaction and weak cation exchange. Moreover, thanks to their throughpores, fast separation at low column backpressure could be achieved in these three modes. Both protein recovery and column stability were satisfactory. The penetrable poly(MAA-co-EDMA) microspheres were potential stationary phase matrix for fast protein separation.

Graphical abstract

image


https://ift.tt/2GZUKYu

An update on the role of irisin in the regulation of endocrine and metabolic functions

Publication date: Available online 30 March 2018
Source:Peptides
Author(s): Mohamed Omer Mahgoub, Crystal D'Souza, Reem S.M.H. AlDarmaki, May M.Y.H. Baniyas, Ernest Adeghate
Irisin is a novel myokine and adipokine that has gained much attention recently due to its mechanisms of action. Irisin is secreted following proteolytic cleavage of its precursor fibronectin type III domain containing 5 (FNDC5). Following its release, irisin exerts its major action by increasing the expression of mitochondrial uncoupling protein 1 (UCP 1), which facilitates the conversion of white adipose tissue (WAT) into beige adipose tissue. Irisin is distributed in various body tissues and several actions have been attributed to its presence in those tissues. It has been suggested that it plays a role in metabolic diseases, ageing, inflammation and neurogenesis. However, the circulating levels of irisin are modulated by several factors such as diet, obesity, exercise, pharmacological agents and different pathological conditions. In this review, we have discussed the mechanisms by which irisin influences the functions of different body systems and how external factors in turn affect the circulating level of irisin. In conclusion, modification of circulating irisin level may help in the management of a variety of endocrine and metabolic disorders.

Graphical abstract

image


https://ift.tt/2uAYXPC

Editorial Board

Publication date: April 2018
Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Volume 1861, Issue 4





https://ift.tt/2GX63At

Multiple roles of lymphatic vessels in tumor progression

S09527915.gif

Publication date: August 2018
Source:Current Opinion in Immunology, Volume 53
Author(s): Qiaoli Ma, Lothar C Dieterich, Michael Detmar
Sentinel lymph node metastasis is a prognostic indicator for systemic tumor spread in many types of cancers, and tumor lymphangiogenesis correlates with reduced survival. Consequently, lymphatic vessels have been suggested to promote tumor progression in multiple ways. Tumor lymphangiogenesis occurs both in primary tumors and at distant (pre-) metastatic sites, and facilitates lymphatic invasion and tumor cell dissemination. Lymphatic vessels have also emerged as regulators of tumor immunity, transporting tumor antigens to lymph nodes and directly interacting with immune cells. Furthermore, lymphatic vessels might provide a 'lymphovascular' niche contributing to the maintenance of stem-like tumor cells that are tightly related to tumor recurrence. Thus, targeting tumor lymphangiogenesis or specific lymphatic-associated functions might represent a promising approach to inhibit tumor progression.



https://ift.tt/2J5SI9E

Next generation natural killer cells for cancer immunotherapy: the promise of genetic engineering

S09527915.gif

Publication date: April 2018
Source:Current Opinion in Immunology, Volume 51
Author(s): May Daher, Katayoun Rezvani
Recent advances in the field of cellular therapy have focused on autologous T cells engineered to express a chimeric antigen receptor (CAR) against tumor antigens. Remarkable responses have been observed in patients receiving autologous CD19-redirected T cells for the treatment of B-lymphoid malignancies. However, the generation of autologous products for each patient is logistically challenging and expensive. Extensive research efforts are ongoing to generate an off-the-shelf cellular product for the treatment of cancer patients. Natural killer (NK) cells are attractive contenders since they have potent anti-tumor activity, and their safety in the allogeneic setting expands the cell sources for NK cell therapy beyond an autologous one. In this review, we discuss advantages and limitations of NK cellular therapy, and novel genetic engineering strategies that may be applied to overcome some of the limitations. Next-generation engineered NK cells are showing great promise in the preclinical setting and it is likely that in the next few years CAR-engineered NK cells will be incorporated into the current armamentarium of cell-based cancer therapeutics.



https://ift.tt/2pTbKaI

Immune privilege disruption in folliculotropic mycosis fungoides: investigation of major histocompatibility complex antigen expression

International Journal of Dermatology, EarlyView.


https://ift.tt/2GHwYm9

Paederus dermatitis – touched by champion flies – three clinical manifestations of pederin toxin‐inflicted dermatitis

International Journal of Dermatology, EarlyView.


https://ift.tt/2GnV6a3

Acupuncture for alcohol use disorder.

Related Articles

Acupuncture for alcohol use disorder.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):60-69

Authors: Chen P, Li J, Han X, Grech D, Xiong M, Bekker A, Ye JH

Abstract
Alcohol use disorder (AUD) is a common medical and social problem, affecting about 240 million people in the world. To address this major health concern, the currently available treatments for AUD need to be improved. Acupuncture, a popular form of complementary and alternative therapy, is emerging as an effective treatment for AUD. This review summarizes how preclinical and clinical studies are related to the application of acupuncture for AUD. These studies suggest that if used correctly, acupuncture may effectively reduce alcohol intake, attenuate alcohol withdrawal syndrome, and rebalance AUD-induced maladaptation in neurotransmitters and hormones in related brain areas. The progress of research in this field is at an early stage. Future investigations with rigorous design and carefully constructed protocols are still needed.

PMID: 29593851 [PubMed]



https://ift.tt/2J8iLNJ

Systemic delivery of selective EP1 and EP3 receptor antagonists attenuates pentylenetetrazole-induced seizures in mice.

Related Articles

Systemic delivery of selective EP1 and EP3 receptor antagonists attenuates pentylenetetrazole-induced seizures in mice.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):47-59

Authors: Reschke CR, Poersch AB, Masson CJ, Jesse AC, Marafiga JR, Lenz QF, Oliveira MS, Henshall DC, Mello CF

Abstract
Neuroinflammation plays a major role in brain excitability and may contribute to the development of epilepsy. Prostaglandin E2 (PGE2) is a direct mediator of inflammatory responses and, through EP receptors, plays an important role in neuronal excitability. Pharmacological evidence supports that centrally-administered EP1 and EP3 receptor antagonists reduced acutely evoked seizures in rats. Translation of these findings would benefit from evidence of efficacy with a more clinically relevant route of delivery and validation in another species. In the current study we investigated whether the systemic administration of EP1 and EP3 agonists and antagonists modulate pentylenetetrazole (PTZ)-induced seizures in mice. In addition, it was examined whether these compounds alter Na+, K+-ATPase activity, an enzyme responsible for the homeostatic ionic equilibrium and, consequently, for the resting membrane potential in neurons. While the systemic administration of EP1 and EP3 antagonists (ONO-8713 and ONO-AE3-240, respectively) attenuated, the respective agonists (ONO-DI-004 and ONO-AE-248) potentiated PTZ-induced seizures (all compounds injected at the dose of 10 µg/kg, s.c., 30 min before PTZ challenge). Co-administration of either EP1 or EP3 agonist with the respective antagonists nullified the anticonvulsant effects of EP1/3 receptor blockade. In addition, EP1 and EP3 agonists exacerbated PTZ-induced decrease of Na+, K+-ATPase activity in both cerebral cortex and hippocampus, whereas, EP1 and EP3 antagonists prevented PTZ-induced decrease of Na+, K+-ATPase activity in both structures. Our findings support and extend evidence that EP1 and EP3 receptors may be novel targets for the development of anticonvulsant drugs.

PMID: 29593850 [PubMed]



https://ift.tt/2pSoxtM

Ascorbic acid and sodium benzoate synergistically aggravates testicular dysfunction in adult Wistar rats.

Related Articles

Ascorbic acid and sodium benzoate synergistically aggravates testicular dysfunction in adult Wistar rats.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):39-46

Authors: Kehinde OS, Christianah OI, Oyetunji OA

Abstract
BACKGROUND: The effect of the concomitant use of sodium benzoate (NaB) and ascorbic acid on human health remains controversial. Therefore, the current study is designed to investigate the effect of NaB and ascorbic acid on the testicular function of adult Wistar rats.
METHODS: Adult Wistar rats were randomly allotted into Control (vehicle; received 1 ml of distilled water), NaB-treated (SB-treated; received 100 mg/kg body weight; b.w), ascorbic acid-treated (AA-treated; received 150 mg/kg b.w) and NaB+ ascorbic acid-treated (SB+AA-treated) groups. The treatment lasted for 28 days and the administration was given orally. The body weight change was monitored. Semen analysis, biochemical assay and histological examination were performed.
RESULTS: Treatment with NaB significantly altered the cytoarchitecture of testicular tissue, sperm quality, testicular endocrine function and oxidative stress status without any alteration in body weight gain compared to control. In addition, treatment with NaB+ ascorbic acid exacerbated testicular tissue disruption, impaired sperm quality and testicular endocrine impairment with significant reduction in oxidative stress and unaltered body weight gain when compared with NaB-treated group.
CONCLUSION: This study suggests that ascorbic acid and NaB synergistically aggravates testicular dysfunction. This is independent of oxidative stress status.

PMID: 29593849 [PubMed]



https://ift.tt/2J7sBz3

Saturated fatty acids bound to albumin enhance osteopontin expression and cleavage in renal proximal tubular cells.

Related Articles

Saturated fatty acids bound to albumin enhance osteopontin expression and cleavage in renal proximal tubular cells.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):29-38

Authors: Cobbs A, Ballou K, Chen X, George J, Zhao X

Abstract
Osteopontin (OPN) is one of the proinflammatory cytokines upregulated in the kidneys of diabetic animals and patients with nephropathy. An increase in urinary albumin and albumin-bound fatty acids (FA) presents a proinflammatory environment to the proximal tubules in proteinuric kidney diseases including diabetic nephropathy. This study was designed to examine if FA overload could stimulate OPN expression and cleavage in renal tubule epithelial cells. OPN gene and protein expression was examined in the kidney of Zucker diabetic (ZD) rats and cultured proximal tubular cells exposed to either bovine serum albumin (BSA) or BSA conjugated with palmitic acid (PA), the most abundant saturated plasma FA. Real-time PCR analysis confirmed an upregulation of renal cortical OPN gene correlated with albuminuria and nephropathy progression in ZD rats at the age of 7-20 weeks. Immunofluorescence staining of kidney sections revealed a massive induction of OPN protein in albumin-overloaded proximal tubules of ZD rats. A significant increase in both intact and cleaved OPN proteins was further demonstrated in the diabetic kidney and urine samples, which was attenuated by antiproteinuric treatment with losartan, an angiotensin II receptor blocker. When exposed to fatty acid-free BSA, NRK-52E cells exhibited an increase in protein levels of full-length and cleaved OPN. Moreover, the increase in OPN fragments was greatly enhanced in the presence of PA (250-500 µM). Together, our results support a stimulatory effect of albumin and conjugated FA on OPN expression and cleavage in renal tubule epithelial cells. Thus, besides lowering albuminuria/proteinuria, mitigating circulating FAs may be an effective intervention for preventing and slowing down the progression of nephropathy associated with obesity and type 2 diabetes.

PMID: 29593848 [PubMed]



https://ift.tt/2pTPRIb

The crosstalk between autonomic nervous system and blood vessels.

Related Articles

The crosstalk between autonomic nervous system and blood vessels.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):17-28

Authors: Sheng Y, Zhu L

Abstract
The autonomic nervous system (ANS), comprised of two primary branches, sympathetic and parasympathetic nervous system, plays an essential role in the regulation of vascular wall contractility and tension. The sympathetic and parasympathetic nerves work together to balance the functions of autonomic effector organs. The neurotransmitters released from the varicosities in the ANS can regulate the vascular tone. Norepinephrine (NE), adenosine triphosphate (ATP) and Neuropeptide Y (NPY) function as vasoconstrictors, whereas acetylcholine (Ach) and calcitonin gene-related peptide (CGRP) can mediate vasodilation. On the other hand, vascular factors, such as endothelium-derived relaxing factor nitric oxide (NO), and constriction factor endothelin, play an important role in the autonomic nervous system in physiologic conditions. Endothelial dysfunction and inflammation are associated with the sympathetic nerve activity in the pathological conditions, such as hypertension, heart failure, and diabetes mellitus. The dysfunction of the autonomic nervous system could be a risk factor for vascular diseases and the overactive sympathetic nerve is detrimental to the blood vessel. In this review, we summarize findings concerning the crosstalk between ANS and blood vessels in both physiological and pathological conditions and hope to provide insight into the development of therapeutic interventions of vascular diseases.

PMID: 29593847 [PubMed]



https://ift.tt/2J7OoH9

New insights into Nod-like receptors (NLRs) in liver diseases.

Related Articles

New insights into Nod-like receptors (NLRs) in liver diseases.

Int J Physiol Pathophysiol Pharmacol. 2018;10(1):1-16

Authors: Xu T, Du Y, Fang XB, Chen H, Zhou DD, Wang Y, Zhang L

Abstract
Activation of inflammatory signaling pathways is of central importance in the pathogenesis of alcoholic liver disease (ALD) and nonalcoholic steatohepatitis (NASH). Nod-like receptors (NLRs) are intracellular innate immune sensors of microbes and danger signals that control multiple aspects of inflammatory responses. Recent studies demonstrated that NLRs are expressed and activated in innate immune cells as well as in parenchymal cells in the liver. For example, NLRP3 signaling is involved in liver ischemia-reperfusion (I/R) injury and silencing of NLRP3 can protect the liver from I/R injury. In this article, we review the evidence that highlights the critical importance of NLRs in the prevalent liver diseases. The significance of NLR-induced intracellular signaling pathways and cytokine production is also evaluated.

PMID: 29593846 [PubMed]



https://ift.tt/2E5Gzhb

Editorial Board

alertIcon.gif

Publication date: April 2018
Source:European Annals of Otorhinolaryngology, Head and Neck Diseases, Volume 135, Issue 2





https://ift.tt/2E7RLKn

Editorial Board

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300





https://ift.tt/2uA36n4

Preface: Special issue on measuring behaviour 2016

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300
Author(s): Gernot Riedel, Cathal Gurrin, Andrew Spink




https://ift.tt/2pT0nAp

Lacking quality in research: Is behavioral neuroscience affected more than other areas of biomedical science?

elsevier-non-solus.png

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300
Author(s): Anton Bespalov, Thomas Steckler
There are many reasons why novel therapeutics fail in clinical trials but these failures are often attributed to lacking quality of preclinical data. These problems are not limited to any specific therapeutic area, academic or industrial research and are due in large part to several generic factors influencing research quality (e.g., related to definition of pre-specified endpoints, principles of study design and analysis, biased reporting, and lack of proper training). Yet, neuroscience drug discovery is often said to be affected more than the other fields. Within neuroscience, behavioral studies are the most blamed for being poorly designed, underpowered and mis-reported and there are indeed several factors that may be rather unique for behavioral research, such as a multitude of environmental conditions that are difficult to control and that are often not reported, ethical concerns about in vivo research and the pressure to reduce animal numbers, contributing to under-powered studies, and the complexity of study design and analysis, creating too much room for post hoc data massaging and selective reporting. Also, the blood-brain barrier as a frequently neglected complicating factor has to be considered in CNS research. The importance of these factors is increasingly recognized and urgent efforts are needed to demonstrate that behavioral methods of preclinical neuroscience research deliver results that can be as robust as with the non-behavioral methods Until this goal is achieved, behavioral neuroscience and neuroscience in general may be losing young talent, CNS drug discovery may lack the needed investment and this field may indeed be amongst the most affected by the current preclinical data quality crisis.



https://ift.tt/2uHeYUr

Between and within laboratory reliability of mouse behaviour recorded in home-cage and open-field

elsevier-non-solus.png

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300
Author(s): Lianne Robinson, Berry Spruijt, Gernot Riedel
BackgroundReproducibility of behavioural findings between laboratories is difficult due to behaviour being sensitive to environmental factors and interactions with genetics. The objective of this study was to investigate reproducibility of behavioural data between laboratories using the PhenoTyper home cage observation system and within laboratory reproducibility using different lighting regimes.New methodThe ambulatory activity of C57BL/6 and DBA/2 mice was tested in PhenoTypers in two laboratories under near identical housing and testing conditions (Exp. 1). Additionally activity and anxiety were also assessed in the open-field test. Furthermore, testing in either a normal or inverted light/dark cycle was used to determine effects of lighting regime in a within-laboratory comparison in Aberdeen (Exp. 2).ResultsUsing the PhenoTyper similar circadian rhythms were observed across laboratories. Higher levels of baseline and novelty-induced activity were evident in Aberdeen compared to Utrecht although strain differences were consistent between laboratories. Open field activity was also similar across laboratories whereas strain differences in anxiety were different. Within laboratory analysis of different lighting regimes revealed that behaviour of the mice was sensitive to changes in lighting.Comparison with existing methodsUtilisation of a home cage observation system facilitates the reproducibility of activity but not anxiety-related behaviours across laboratories by eliminating environmental factors known to influence reproducibility in standard behavioural tests.ConclusionsStandardisation of housing/test conditions resulted in reproducibility of home cage and open field activity but not anxiety-related phenotypes across laboratories with some behaviours more sensitive to environmental factors. Environmental factors include lighting and time of day.



https://ift.tt/2pRXDD7

High tech cognitive and acoustic enrichment for captive elephants

elsevier-non-solus.png

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300
Author(s): Fiona French, Clara Mancini, Helen Sharp
This paper investigates the potential for using technology to support the development of sensory and cognitive enrichment activities for captive elephants. It explores the usefulness of applying conceptual frameworks from interaction design and game design to the problem of developing species-specific smart toys that promote natural behaviours and provide stimulation. We adopted a Research through Design approach, and describe how scientific inquiry supported our design process, while the creation of artefacts guided our investigations into possible future solutions. Our fieldwork resulted in the development of an interactive prototype of an acoustic toy that elephants are able to control using interface elements constructed from a range of natural materials.



https://ift.tt/2IhBs04

Extended, continuous measures of functional status in community dwelling persons with Alzheimer’s and related dementia: Infrastructure, performance, tradeoffs, preliminary data, and promise

elsevier-non-solus.png

Publication date: 15 April 2018
Source:Journal of Neuroscience Methods, Volume 300
Author(s): Bradley Zylstra, George Netscher, Julien Jacquemot, Michael Schaffer, Galen Shen, Angela D. Bowhay, Tamara L. Braley, Katherine L. Possin, Bruce L. Miller, Alexandre M. Bayen, Stephen J. Bonasera, A. Katrin Schenk
BackgroundThe past decades have seen phenomenal growth in the availability of inexpensive and powerful personal computing devices. Efforts to leverage these devices to improve health care outcomes promise to remake many aspects of healthcare delivery, but remain in their infancy.New methodWe describe the development of a mobile health platform designed for daily measures of functional status in ambulatory, community dwelling subjects, including those who have Alzheimer's disease or related neurodegenerative disorders. Using Smartwatches and Smartphones we measure subject overall activity and outdoor location (to derive their lifespace). These clinically-relevant measures allow us to track a subject's functional status in their natural environment over prolonged periods of time without repeated visits to healthcare providers. Functional status metrics are integrated with medical information and caregiver reports, which are used by a caregiving team to guide referrals for physician/APRN/NP care.Comparisonwith Existing Methods We describe the design tradeoffs involved in all aspects of our current system architecture, focusing on decisions with significant impact on system cost, performance, scalability, and user-adherence.ResultsWe provide real-world data from current subject enrollees demonstrating system accuracy and reliability.ConclusionsWe document real-world feasibility in a group of men and women with dementia that Smartwatches/Smartphones can provide long-term, relevant clinical data regarding individual functional status. We describe the underlying considerations of this system so that interested organizations can adapt and scale our approach to their needs. Finally, we provide a potential agenda to guide development of future systems.



https://ift.tt/2pOSZGc

Effect of IFN-λ2 on combined allergic rhinitis with nasal polyps

OBJECTIVE: This study sought to investigate the expression of interferon-λ2 (IFN-λ2) in patients with combined allergic rhinitis and nasal polyps (AR+NP), analyze the correlation between IFN-λ2 and tryptase, interleukin 10 (IL-10), and interleukin 12 (IL-12), and identify its peripheral blood cell origins.

PATIENTS AND METHODS: ELISA kits were used to investigate plasma levels of IFN-λ2, tryptase, IL-10, and IL-12 in AR+NP patients and healthy controls (HC). Flow cytometry analysis was carried out to detect IFN-λ2 expression in peripheral blood leukocytes. Immunocytochemical staining was performed to detect nasal polyp IFN-λ2 expression in AR+NP patients.

RESULTS: Elevated plasma IFN-λ2 levels and positive correlations between plasma IFN-λ2 and tryptase levels in AR+NP patients indicated that IFN-λ2 likely contributes to AR+NP pathogenesis. IFN-λ2 expression was upregulated in cytotoxic T cells and eosinophils in AR+NP patients. Nasal polyp mast cells and macrophages in AR+NP patients expressed IFN-λ2.

CONCLUSIONS: The close correlation between IFN-λ2 expression and AR+NP may provide experimental evidence for a possible effect of IFN-λ2 against the allergic inflammatory reaction. Therefore, IFN-λ2 actions may have a potential utility for the treatment and prevention of AR+AP.

L'articolo Effect of IFN-λ2 on combined allergic rhinitis with nasal polyps sembra essere il primo su European Review.



https://ift.tt/2Gp4ydt

Cost‐effectiveness of sialendoscopy versus medical management for radioiodine‐induced sialadenitis

The Laryngoscope, EarlyView.


https://ift.tt/2GrvER7

Post‐acute care use after major head and neck oncologic surgery with microvascular reconstruction

The Laryngoscope, EarlyView.


https://ift.tt/2GK7yV8

Allergic phenotype of chronic rhinosinusitis based on radiologic pattern of disease

The Laryngoscope, EarlyView.


https://ift.tt/2GrXJHN

Transoral resection of extensive pediatric supraglottic neurofibroma

The Laryngoscope, EarlyView.


https://ift.tt/2GK7vsq

The influence of hypoxia and IFN-γ on the proteome and metabolome of therapeutic mesenchymal stem cells

elsevier-non-solus.png

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Holly M. Wobma, Manuel A. Tamargo, Shahar Goeta, Lewis M. Brown, Raimon Duran-Struuck, Gordana Vunjak-Novakovic
Over the past 15 years, mesenchymal stem cells (MSCs) have been assessed for their capacity to suppress inflammation and promote tissue repair. Regardless of whether the cells are primed (exposed to instructive cues) before administration, their phenotype will respond to environmental signals present in the pathophysiological setting being treated. Since hypoxia and inflammation coexist in the settings of acute injury and chronic disease we sought to explore how the proteome and metabolome of MSCs changes when cells were exposed to 48 h of 1% oxygen, interferon gamma (IFN-γ), or both cues together. We specifically focused on changes in cell metabolism, immune modulation, extracellular matrix secretion and modification, and survival capacity. IFN-γ promoted expression of anti-pathogenic proteins and induced MSCs to limit inflammation and fibrosis while promoting their own survival. Hypoxia instead led to cell adaptation to low oxygen, including upregulation of proteins involved in anaerobic metabolism, autophagy, angiogenesis, and cell migration. While dual priming resulted in additive effects, we also found many instances of synergy. These data lend insight to how MSCs may behave after administration to a patient and suggest how priming cells beforehand could improve their therapeutic capacity.



https://ift.tt/2pS9oJU

Near infrared-emitting persistent luminescent nanoparticles for Hepatocellular Carcinoma imaging and luminescence-guided surgery

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Ting Ai, Wenting Shang, Hao Yan, Chaoting Zeng, Kun Wang, Yuan Gao, Tianpei Guan, Chihua Fang, Jie Tian
Hepatocellular carcinoma (HCC), the fifth most common cancer worldwide, is increasing nowadays and poses a serious threat to human health. However, if treated effectively and timely, it is clinically manageable or curable. Therefore, accurate detection and complete surgical resection remain priorities for HCC with a high potential of improving both survival and quality of life. Lacking of real-time guide technology, traditional surgery are usually relied on the subjective experience of surgeon, which have the limitation of high sensitivity detection tumor. Here, we developed a contrast agent, ZnGa2O4Cr0.004 (ZGC), used for guided surgery during operation to accurate delineation of HCC. ZGC showed excellent long-lasting afterglow properties that lasted for hours, which can aid in real-time guided surgery. Meanwhile, ZGC display high spatial resolution and deep penetration during pre-operation for diagnostic computed tomography (CT). Interestingly, we observed reverse imaging in the tumor region, known as a "dark hole", which further improves the contrast for surgery. This new multi-modality nanoparticle has great potential for accurate liver cancer imaging and resection guidance.

Graphical abstract

image


https://ift.tt/2uzhLPd

Nucleosome-inspired nanocarrier obtains encapsulation efficiency enhancement and side effects reduction in chemotherapy by using fullerenol assembled with doxorubicin

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Jinglong Tang, Ruirui Zhang, Mengyu Guo, Leihou Shao, Ying Liu, Yuliang Zhao, Suojiang Zhang, Yan Wu, Chunying Chen
Chemodrugs have been widely used to treat cancer; however, the chemotherapy usually leads to serious side effects and failure. Various nanomaterials and strategies have been explored for drug delivery to improve the efficacy of chemodrugs. One key to loading chemodrugs onto a nano-delivery system is enhancement of the encapsulation efficiency, especially for polymeric nanoparticles being loaded with hydrophilic drugs. Inspired by the ability of eukaryote to package millions of genes in the nucleus wrapping and condensing DNA around histones to form chromosomes, here we developed a karyon-like hybrid nanoparticle to achieve ultra-high encapsulation of doxorubicin (Dox) with reduced side effects. We utilized fullerenol as a "histone", packaged a great number of Dox, and used PEG-PLGA as the "karyotheca" coating the "nucleosome" (fullerenol and Dox complex) to stabilize the complex. It is noteworthy that the encapsulation efficiency of Dox in the polymeric micelles was increased from ∼5% to ∼79%. What's more, the biomimetic-inspired delivery system significantly reduced the chemodrug side effects by utilizing the radical scavenging ability of fullerenol. This novel drug-delivery design approach provides useful insights for improving the applicability of fullerenol in drug delivery systems for cancer therapy.

Graphical abstract

image


https://ift.tt/2pRTajM

The influence of hypoxia and IFN-γ on the proteome and metabolome of therapeutic mesenchymal stem cells

elsevier-non-solus.png

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Holly M. Wobma, Manuel A. Tamargo, Shahar Goeta, Lewis M. Brown, Raimon Duran-Struuck, Gordana Vunjak-Novakovic
Over the past 15 years, mesenchymal stem cells (MSCs) have been assessed for their capacity to suppress inflammation and promote tissue repair. Regardless of whether the cells are primed (exposed to instructive cues) before administration, their phenotype will respond to environmental signals present in the pathophysiological setting being treated. Since hypoxia and inflammation coexist in the settings of acute injury and chronic disease we sought to explore how the proteome and metabolome of MSCs changes when cells were exposed to 48 h of 1% oxygen, interferon gamma (IFN-γ), or both cues together. We specifically focused on changes in cell metabolism, immune modulation, extracellular matrix secretion and modification, and survival capacity. IFN-γ promoted expression of anti-pathogenic proteins and induced MSCs to limit inflammation and fibrosis while promoting their own survival. Hypoxia instead led to cell adaptation to low oxygen, including upregulation of proteins involved in anaerobic metabolism, autophagy, angiogenesis, and cell migration. While dual priming resulted in additive effects, we also found many instances of synergy. These data lend insight to how MSCs may behave after administration to a patient and suggest how priming cells beforehand could improve their therapeutic capacity.



https://ift.tt/2pS9oJU

Near infrared-emitting persistent luminescent nanoparticles for Hepatocellular Carcinoma imaging and luminescence-guided surgery

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Ting Ai, Wenting Shang, Hao Yan, Chaoting Zeng, Kun Wang, Yuan Gao, Tianpei Guan, Chihua Fang, Jie Tian
Hepatocellular carcinoma (HCC), the fifth most common cancer worldwide, is increasing nowadays and poses a serious threat to human health. However, if treated effectively and timely, it is clinically manageable or curable. Therefore, accurate detection and complete surgical resection remain priorities for HCC with a high potential of improving both survival and quality of life. Lacking of real-time guide technology, traditional surgery are usually relied on the subjective experience of surgeon, which have the limitation of high sensitivity detection tumor. Here, we developed a contrast agent, ZnGa2O4Cr0.004 (ZGC), used for guided surgery during operation to accurate delineation of HCC. ZGC showed excellent long-lasting afterglow properties that lasted for hours, which can aid in real-time guided surgery. Meanwhile, ZGC display high spatial resolution and deep penetration during pre-operation for diagnostic computed tomography (CT). Interestingly, we observed reverse imaging in the tumor region, known as a "dark hole", which further improves the contrast for surgery. This new multi-modality nanoparticle has great potential for accurate liver cancer imaging and resection guidance.

Graphical abstract

image


https://ift.tt/2uzhLPd

Nucleosome-inspired nanocarrier obtains encapsulation efficiency enhancement and side effects reduction in chemotherapy by using fullerenol assembled with doxorubicin

Publication date: June 2018
Source:Biomaterials, Volume 167
Author(s): Jinglong Tang, Ruirui Zhang, Mengyu Guo, Leihou Shao, Ying Liu, Yuliang Zhao, Suojiang Zhang, Yan Wu, Chunying Chen
Chemodrugs have been widely used to treat cancer; however, the chemotherapy usually leads to serious side effects and failure. Various nanomaterials and strategies have been explored for drug delivery to improve the efficacy of chemodrugs. One key to loading chemodrugs onto a nano-delivery system is enhancement of the encapsulation efficiency, especially for polymeric nanoparticles being loaded with hydrophilic drugs. Inspired by the ability of eukaryote to package millions of genes in the nucleus wrapping and condensing DNA around histones to form chromosomes, here we developed a karyon-like hybrid nanoparticle to achieve ultra-high encapsulation of doxorubicin (Dox) with reduced side effects. We utilized fullerenol as a "histone", packaged a great number of Dox, and used PEG-PLGA as the "karyotheca" coating the "nucleosome" (fullerenol and Dox complex) to stabilize the complex. It is noteworthy that the encapsulation efficiency of Dox in the polymeric micelles was increased from ∼5% to ∼79%. What's more, the biomimetic-inspired delivery system significantly reduced the chemodrug side effects by utilizing the radical scavenging ability of fullerenol. This novel drug-delivery design approach provides useful insights for improving the applicability of fullerenol in drug delivery systems for cancer therapy.

Graphical abstract

image


https://ift.tt/2pRTajM

Neonatal Transitions in Social Behavior and Their Implications for Autism

Publication date: Available online 30 March 2018
Source:Trends in Cognitive Sciences
Author(s): Sarah Shultz, Ami Klin, Warren Jones
Within the context of early infant–caregiver interaction, we review a series of pivotal transitions that occur within the first 6 months of typical infancy, with emphasis on behavior and brain mechanisms involved in preferential orientation towards, and interaction with, other people. Our goal in reviewing these transitions is to better understand how they may lay a necessary and/or sufficient groundwork for subsequent phases of development, and also to understand how the breakdown thereof, when development is atypical and those transitions become derailed, may instead yield disability. We review these developmental processes in light of recent studies documenting disruptions to early-emerging brain and behavior mechanisms in infants later diagnosed with autism spectrum disorder, shedding light on the brain–behavior pathogenesis of autism.



https://ift.tt/2uzAKsP

Interpreting and Utilising Intersubject Variability in Brain Function

Publication date: Available online 30 March 2018
Source:Trends in Cognitive Sciences
Author(s): Mohamed L. Seghier, Cathy J. Price
We consider between-subject variance in brain function as data rather than noise. We describe variability as a natural output of a noisy plastic system (the brain) where each subject embodies a particular parameterisation of that system. In this context, variability becomes an opportunity to: (i) better characterise typical versus atypical brain functions; (ii) reveal the different cognitive strategies and processing networks that can sustain similar tasks; and (iii) predict recovery capacity after brain damage by taking into account both damaged and spared processing pathways. This has many ramifications for understanding individual learning preferences and explaining the wide differences in human abilities and disabilities. Understanding variability boosts the translational potential of neuroimaging findings, in particular in clinical and educational neuroscience.



https://ift.tt/2pRrdc6

Measuring feeding difficulties in toddlers with Down syndrome

S01956663.gif

Publication date: 1 July 2018
Source:Appetite, Volume 126
Author(s): Marijn van Dijk, Wilma Lipke-Steenbeek
Early feeding problems occur frequently across the population, but have a higher incidence in children with Down syndrome (DS). Early identification can possibly be improved with the help of a valid screening instrument based on caregiver reports. In a previous study, we investigated the concurrent validity of the Dutch version of the Montreal Children's Hospital Feeding Scale (MCH-FS, SEP in Dutch) in a sample of typically developing toddlers, and we found a correlation between the score on the instrument and observed behavior during a regular meal. The current pilot study was a replication in a sample of children with DS (aged 1; 0–3; 0) and their primary caregivers (n = 32).The results showed that children in the sample did not score higher on the SEP than children in their respective norm groups. In addition, when caregivers reported more symptoms of feeding problems on the SEP, children showed more food refusal and negative affect during the observed meal. This suggests that the screening instrument is particularly associated with negative mealtime interactions. This is in contrast with earlier results, which mainly indicated a relation with eating skills.



https://ift.tt/2pRQpPs

Retraining of automatic action tendencies in individuals with obesity: A randomized controlled trial

S01956663.gif

Publication date: 1 July 2018
Source:Appetite, Volume 126
Author(s): Hannah Ferentzi, Hannah Scheibner, Reinout Wiers, Eni S. Becker, Johannes Lindenmeyer, Sylvia Beisel, Mike Rinck
Obesity is a major health concern, characterized by an automatically activated tendency to (over)-eat. Recent research suggests that an effective way to counteract automatic approach tendencies in unhealthy consumption behavior might be approach bias modification. Therefore, we investigated an approach-avoidance training for unhealthy food cues in 189 patients with obesity of a psychosomatic inpatient clinic who were participating in a nutrition advice program. Patients in the active training group were trained to make avoidance movements (pushing a joystick) in response to unhealthy food pictures and approach movements (pulling the joystick) in response to positive pictures, while the control group received sham training (approaching and avoiding both picture types). Approach-avoidance bias, body mass index, eating pathology and food-specific implicit associations were assessed before and after the training. In line with our hypothesis, approach-avoidance bias improved in the active training group after the training, in comparison to the sham training group. Moreover, this effect generalized to new, untrained stimuli. However, no effects of the training were found in a food-specific Single-Target Implicit Association Test, or on eating pathology questionnaires or body mass index. While the training results are promising, the effect of approach-avoidance bias modification on relevant behavior in obesity has yet to be established before it may be implemented as an add-on treatment.



https://ift.tt/2uxlSLR

Optimizing outcomes with polymethylmethacrylate fillers

Journal of Cosmetic Dermatology, EarlyView.


https://ift.tt/2GEPJqq

Optimizing outcomes with polymethylmethacrylate fillers

Journal of Cosmetic Dermatology, EarlyView.


https://ift.tt/2GEPJqq

Biologics for pityriasis rubra pilaris treatment: a review of the literature

Pityriasis rubra pilaris (PRP) is a rare inflammatory papulosquamous skin disease, often refractory to conventional therapies. The off-label use of biologics, such as anti-TNF, -IL-12/IL-23, -IL-17 agents, has been proven successful, in the last two decades, in PRP treatment.Our aim was to analyse the literature for the use of biologics in PRP treatment. We conducted a review by "Pubmed" and "clinicaltrial.gov" searches. 68 articles met our selection criteria and were herein discussed. Out of 86 PRP patients, the vast majority of which treated with anti-TNF, -IL-12/IL-23, and -IL-17 biologics, either alone or in combination therapy, a marked-to-complete response (50-78%), a partial response (11-25%) or no/poor response (11-25%) was observed.

https://ift.tt/2Gm2UgD

Comparison of ethylenediaminetetraacetic acid-treated desmoglein ELISA and conventional desmoglein ELISA in the evaluation of pemphigus vulgaris in remission



https://ift.tt/2uEMFFM

Dual neutralization of both IL-17A and IL-17F with bimekizumab in patients with psoriasis: results from BE ABLE 1, a 12-week randomized, double-blinded placebo-controlled phase 2b trial

Neutralizing interleukin (IL)-17F in addition to IL-17A may provide a more complete and specific approach to inhibiting inflammation.

https://ift.tt/2Gm2S8v

Étude de faisabilité de la spirométrie en médecine générale

S07618425.gif

Publication date: Available online 29 March 2018
Source:Revue des Maladies Respiratoires
Author(s): L. Bunge, D. Baruch, L. Plantier, T. Mazars, N. Roche, A. Izadifar
La bronchopneumopathie chronique obstructive est une maladie fréquente insuffisamment dépistée par les médecins généralistes (MG) alors que la spirométrie leur est accessible. L'objectif de ce travail était d'évaluer la qualité et l'interprétation des spirométries réalisées par des internes de médecine générale après une formation courte. Trois internes ont été formés pendant une demi-journée à la spirométrie. Dans 5 cabinets de médecine générale, une spirométrie était proposée à tous les fumeurs de plus de 35 ans. Les examens ont été relus en aveugle par un pneumologue expert indépendant pour évaluer leur qualité et leur interprétation. Des 184 patients éligibles, 89 % ont accepté de participer et 66 % (107) sont venus au 2e rendez-vous. Le pneumologue a évalué la qualité des spirométries comme bonne pour 72 %, moyenne pour 20 % et non interprétable pour 8 %. L'interprétation était correcte pour 91 % des tests. Le coefficient Kappa de concordance de l'interprétation entre MG et expert était de 0,93. Ces tests ont permis de dépister des troubles ventilatoires obstructifs dans 17,5 % des cas. Le temps moyen d'une consultation avec spirométrie était de 19minutes. La consultation dédiée de spirométrie est bien acceptée par les patients en MG. Une formation courte reste à structurer pour permettre la réalisation et l'interprétation de spirométries en MG. Cette étude doit être étendue de façon à mieux étudier la reproductibilité en cas de courbe anormale.COPD is common but is under-diagnosed by general practitioners (GP). GP have a major role in the early diagnosis of this disease. GP could have access to spirometry. The aim of this study was to evaluate the quality and interpretation of spirometry performed by primary care residents following a short education session. Three residents were trained in spirometry for half a day. They then performed spirometry on all smokers over the age of 35 visiting five general practices. The results were reviewed blindly by an independent specialist pulmonologist to assess their quality and interpretation. Among 184 eligible patients, 89% agreed to participate and 66% (n=107) came for the second appointment. The pulmonologist evaluated the quality of spirometry as good in 72% of cases, of suboptimal but acceptable quality in 20% and of poor quality in 8%. Interpretation was accurate in 91% of tests. The Kappa concordance coefficient between GPs and the expert was 0.93. Airflow obstruction was detected in 17.5% of the screened subjects. The average time for a consultation with spirometry was 19minutes. The consultation dedicated to spirometry was well accepted by patients. A short training has to be structured to allow GPs to perform and interpret spirometry properly. This work needs to be extended to better assess reproducibility in cases of abnormal spirometry.



https://ift.tt/2GmJjbX

Influence of Class V preparation on in vivo temperature rise in anesthetized human pulp during exposure to a Polywave® LED light curing unit

S01095641.gif

Publication date: Available online 29 March 2018
Source:Dental Materials
Author(s): Driellen Christine Zarpellon, Patrício Runnacles, Cristiane Maucoski, Dayane Jaqueline Gross, Ulisses Coelho, Frederick Allen Rueggeberg, Cesar Augusto Galvão Arrais
ObjectiveThis in vivo study evaluated pulp temperature (PT) rise in human premolars having deep Class V preparations during exposure to a light curing unit (LCU) using selected exposure modes (EMs).MethodsAfter local Ethics Committee approval, intact first premolars (n=8) requiring extraction for orthodontic reasons, from 8 volunteers, received infiltrative and intraligamental anesthesia and were isolated using rubber dam. A minute pulp exposure was attained and sterile probe from a wireless, NIST-traceable, temperature acquisition system was inserted into the coronal pulp chamber to continuously monitor PT (°C). A deep buccal Class V preparation was prepared using a high speed diamond bur under air-water spray cooling. The surface was exposed to a Polywave® LED LCU (Bluephase 20i, Ivoclar Vivadent) using selected EMs, allowing 7-min span between each exposure: 10-s in low (10-s/L), 10-s (10-s/H), 30-s (30-s/H), or 60-s (60-s/H) in high mode; and 5-s-Turbo (5-s/T). Peak PT values and PT increases over physiologic baseline levels (ΔT) were subjected to 1-way, repeated measures ANOVAs, and Bonferroni's post-hoc tests (α=0.05). Linear regression analysis was performed to establish the relationship between applied radiant exposure and ΔT.ResultsAll EMs produced higher peak PT than the baseline temperature (p<0.001). Only 60-s/H mode generated an average ΔT of 5.5°C (p<0.001). A significant, positive relationship was noted between applied radiant exposure and ΔT (r2=0.8962; p<0.001).SignificanceIn vivo exposure of deep Class V preparation to Polywave® LED LCU increases PT to values considered safe for the pulp, for most EMs. Only the longest evaluated EM caused higher PT increase than the critical ΔT, thought to be associated with pulpal necrosis.



https://ift.tt/2IhX7Fv

Concomitant BCORL1 and BRAF Mutations in Vemurafenib-Resistant Melanoma Cells

Publication date: May 2018
Source:Neoplasia, Volume 20, Issue 5
Author(s): Luca Mologni, Mariantonia Costanza, Geeta Geeta Sharma, Michela Viltadi, Luca Massimino, Stefania Citterio, Stefania Purgante, Hima Raman, Alessandra Pirola, Massimo Zucchetti, Rocco Piazza, Carlo Gambacorti-Passerini
BRAF is the most frequently mutated gene in melanoma. Constitutive activation of mutant BRAFV600E leads to aberrant Ras-independent MAPK signaling and cell transformation. Inhibition of mutant BRAF is a current frontline therapy for such cases, with improved survival compared with chemotherapy. Unfortunately, reactivation of MAPK signaling by several mechanisms has been shown to cause drug resistance and disease recurrence. In this work, we describe the co-occurrence of an in-frame deletion within an amplified BRAFV600E locus and a missense point mutation of the transcriptional repressor BCORL1 in vemurafenib-resistant A375 melanoma cells. Functional data confirmed that truncated p47BRAFV600E and mutant BCORL1Q1076H both contribute to resistance. Interestingly, either endogenous BCORL1 silencing or ectopic BCORL1Q1076H expression mimicked the effects of a CRISPR/Cas9-edited BCORL1Q1076H locus, suggesting a complex mixture of loss- and gain-of-function effects caused by the mutation. Transcriptomic data confirmed this hypothesis. Finally, we show that the pan-RAF inhibitor sorafenib is not affected by expression of BRAF deletion variant and effectively synergizes with vemurafenib to block resistant cells, suggesting a possible intervention for this class of mutants.



https://ift.tt/2pRCl8D

Αρχειοθήκη ιστολογίου