Dermoscopy of folliculosebaceous cystic hamartoma

Australasian Journal of Dermatology, EarlyView.


https://ift.tt/2O2VzS2

Delayed angioedema of the unilateral tongue associated with angiotensin II receptor blocker in a patient with polypharmacy

Australasian Journal of Dermatology, EarlyView.


https://ift.tt/2OL7pWl

Volume outlier benchmark proposal for Australian Mohs surgery

Australasian Journal of Dermatology, EarlyView.


https://ift.tt/2O2YEBJ

Children with unilateral cochlear nerve canal stenosis have bilateral cochleovestibular anomalies

The Laryngoscope, EarlyView.


https://ift.tt/2PTiIrS

Safety and efficacy of Early injection laryngoplasty in pediatric patients

The Laryngoscope, EarlyView.


https://ift.tt/2D29yqq

Correction to: human periodontal ligament fibroblasts stimulated by nanocrystalline hydroxyapatite paste or enamel matrix derivative. An in vitro assessment of PDL attachment, migration, and proliferation

In the original published version of this article, the middle name for author Mirko H. H. Schmidt is missing.

https://ift.tt/2RcB5s8

Damage-regulated autophagy modulator 1 in oral inflammation and infection

Abstract

Objectives

Damage-regulated autophagy modulator (DRAM) 1 is a p53 target gene with possible involvement in oral inflammation and infection. This study sought to examine the presence and regulation of DRAM1 in periodontal diseases.

Material and methods

In vitro, human periodontal ligament fibroblasts were exposed to interleukin (IL)-1β and Fusobacterium nucleatum for up to 2 days. The DRAM1 synthesis and its regulation were analyzed by real-time PCR, immunocytochemistry, and ELISA. Expressions of other autophagy-associated genes were also studied by real-time PCR. In vivo, synthesis of DRAM1 in gingival biopsies from rats and patients with and without periodontal disease was examined by real-time PCR and immunohistochemistry. For statistics, ANOVA and post-hoc tests were applied (p < 0.05).

Results

In vitro, DRAM1 was significantly upregulated by IL-1β and F. nucleatum over 2 days and a wide range of concentrations. Additionally, increased DRAM1 protein levels in response to both stimulants were observed. Autophagy-associated genes ATG3, BAK1, HDAC6, and IRGM were also upregulated under inflammatory or infectious conditions. In vivo, the DRAM1 gene expression was significantly enhanced in rat gingival biopsies with induced periodontitis as compared to control. Significantly increased DRAM1 levels were also detected in human gingival biopsies from sites of periodontitis as compared to healthy sites.

Conclusion

Our data provide novel evidence that DRAM1 is increased under inflammatory and infectious conditions in periodontal cells and tissues, suggesting a pivotal role of DRAM1 in oral inflammation and infection.

Clinical relevance

DRAM1 might be a promising target in future diagnostic and treatment strategies for periodontitis.

https://ift.tt/2F1nlvh