Adipose‐derived stem cells attenuate atopic dermatitis‐like skin lesions in NC/Nga mice

Abstract

There is an unmet need in novel therapeutics for atopic dermatitis (AD). We examined the effects of autologous adipose‐derived stem cells (ADSCs) on AD‐like skin lesions induced by the application of 2,4‐dinitrochlorobenzene (DNCB) in NC/Nga mice. Autologous ADSCs and ADSC‐conditioned medium (ADSC‐CM) were injected intralesionally three times. Clinical severity and histopathologic findings were compared in sham naïve control, saline‐treated, ADSC‐treated, ADSC‐CM‐treated, and 2.5% cortisone lotion‐applied animals. The severity index, skin thickness, mast cell number, as well as expression levels of thymic stromal lymphopoietin, CD45, chemoattractant receptor‐homologous molecule, chemokine ligand 9, and chemokine ligand 20 were significantly lower in mice treated with ADSC, ADSC‐CM, or 2.5% cortisone lotion.

Tissue levels of interferon‐ γ as well as serum levels of interleukin‐33 and immunoglobulin E levels were also decreased in those groups. We conclude that autologous ADSCs improved DNCB‐induced AD‐like skin lesions in NC/Nga mice by reducing inflammation associated with Th2 immune response and interferon‐γ.

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Methodological issues in meta-analysis of the metformin effects on simple obesity

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Parental Expectation and Perception of CI Benefits in Their Implanted Wards

Abstract

Parents of implanted children develop a set of expectations prior to implantation about future performance and outcomes of their child. This set of expectations has an important impact on post-implant satisfaction levels. The position of the parent in the household makes them uniquely placed to assess the impact of implantation in the context within which the child grows up. There is strong need to find out expectations of parents from CI outcomes in implanted children. The functional benefits of CI may be measured by administering the parental perception of CI benefits in their implanted wards. The primary aim of the study was to find out different expectations of CI outcomes in parents of CI users and the perception of CI outcomes in the parents of CI users. Secondary aim was to find out the most important concerns of parents towards their implanted children. Twenty families with a child implanted for duration of more than 2 years were selected for the study. Two questionnaires (1) Parent Expectations Questionnaire for Cochlear Implants (Nemours children clinic) and (2) Parents and their cochlear implanted child Questionnaire (O'Neill Int J Pediatr Otorhinolaryngol 68: 149–160 2004) were administered on these parents. Results were analyzed qualitatively. Parents expected their implanted children to use the telephone, to be able to detect soft sounds, to listen in crowds, to be able to easily understand others, and to show improvement in communication skills. The quality of speech, process of implantation, to take time off for the appointments at the implant centre and making decision to proceed with implantation were main concerns of the parents. Parents have a high expectation from their implanted children in all domains of life i.e. communication abilities, social and academic skills. Parents have high positive views about the outcomes of the CI. However there are areas which need attention for a positive outcome from the point of view of parents of implanted children.

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Obstructive sleep apnea in children and adolescents with and without obesity

Abstract

Purpose

To investigate the prevalence of obstructive sleep apnea (OSA) in children referred for obesity treatment, and to compare the prevalence with that of a normal-weight group. Moreover, we examined the association between Body Mass Index Standard Deviation Score (BMI SDS) and the Apnea–Hypopnea Index (AHI).

Methods

This cross-sectional study included 139 children aged 7–18 years with overweight/obesity (BMI SDS >1.28) recruited from an obesity treatment clinic. The normal-weight group consisted of 33 children (BMI SDS ≤ 1.28) aged 7–18 years recruited from schools. Sleep examinations were performed using a type 3 portable sleep monitor (Nox T3). OSA was defined as AHI ≥ 2. Height and weight were measured and the tonsillar size was clinically estimated using the Brodsky scale.

Results

The OSA prevalence was 44.6% in children with overweight/obesity compared with 9.1% in the normal-weight group (p = 0.0002), and the relative risk of OSA was 4.9 (95% CI 1.6–14.7). In a logistic regression, a one-unit increase in the BMI SDS increased the odds of having OSA by a factor of 1.92 independent of age, sex, tonsillar hypertrophy, and asthma (95% CI 1.33–2.76, p = 0.0005). A generalized linear regression adjusted for the same variables revealed an association between BMI SDS and AHI (a one-unit increase in the BMI SDS equaled an average increase in the AHI of 35% (95% CI 19–53%, p < 0.0001)).

Conclusions

In this study, children with overweight/obesity had a significantly higher prevalence of OSA compared with a normal-weight group. Increased BMI SDS was associated with increased AHI.

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Methodological issues in meta-analysis of the metformin effects on simple obesity

http://bit.ly/2RQWwna

Parental Expectation and Perception of CI Benefits in Their Implanted Wards

Abstract

Parents of implanted children develop a set of expectations prior to implantation about future performance and outcomes of their child. This set of expectations has an important impact on post-implant satisfaction levels. The position of the parent in the household makes them uniquely placed to assess the impact of implantation in the context within which the child grows up. There is strong need to find out expectations of parents from CI outcomes in implanted children. The functional benefits of CI may be measured by administering the parental perception of CI benefits in their implanted wards. The primary aim of the study was to find out different expectations of CI outcomes in parents of CI users and the perception of CI outcomes in the parents of CI users. Secondary aim was to find out the most important concerns of parents towards their implanted children. Twenty families with a child implanted for duration of more than 2 years were selected for the study. Two questionnaires (1) Parent Expectations Questionnaire for Cochlear Implants (Nemours children clinic) and (2) Parents and their cochlear implanted child Questionnaire (O'Neill Int J Pediatr Otorhinolaryngol 68: 149–160 2004) were administered on these parents. Results were analyzed qualitatively. Parents expected their implanted children to use the telephone, to be able to detect soft sounds, to listen in crowds, to be able to easily understand others, and to show improvement in communication skills. The quality of speech, process of implantation, to take time off for the appointments at the implant centre and making decision to proceed with implantation were main concerns of the parents. Parents have a high expectation from their implanted children in all domains of life i.e. communication abilities, social and academic skills. Parents have high positive views about the outcomes of the CI. However there are areas which need attention for a positive outcome from the point of view of parents of implanted children.

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An efficient method to determine the Hydrophile‐lipophile Balance of surfactants using the phase inversion temperature deviation of CiEj/n‐octane/water emulsions

Abstract

Objective

The aim of this study was to develop a fast and an efficient method to determine the Hydrophile‐Lipophile Balance (HLB) number of cosmetic and pharmaceutics surfactants.

Methods

This method is based on the deviation of the phase inversion temperature induced by the addition of the test compound, with respect to the phase inversion temperature of a reference system, which includes an ethoxylated surfactant. This method is called PIT‐deviation.

Results

Three calibration curves are set up with three reference ethoxylated surfactants. These calibration curves make it possible to evaluate the interfacial behavior of certain chemicals. More particularly, these curves make it possible to easily determine the surfactant HLB.

Conclusion

In this study, a fast and accurate method has been developed to determine the hydrophilic‐lipophilic balance (HLB) number of amphiphilic chemicals. This new method can be applied to establish an HLB number of all commercial amphiphilic ingredients. Compounds which have a PIT‐deviation close to zero are also discussed.

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