The efficacy of cotton ball packing after endoscopic sinus surgery: A prospective, randomized, controlled trial

Publication date: Available online 17 February 2019

Source: Auris Nasus Larynx

Author(s): Seon-Lin Kim, Sung-Dong Kim, Han-Seul Na, Jae-Wook Kim, Keun-Ik Yi, Sue-Jean Mun, Kyu-Sup Cho

Abstract

Objective

The blocking of airflow into sinonasal cavity may decrease postoperative crusting and the development of adhesions. The purpose of this study was to investigate the efficacy of cotton ball packing in patients following endoscopic sinus surgery (ESS).

Methods

Thirty nine patients with chronic rhinosinusitis requiring the same extent of ESS were included. As a part of postoperative care, the patients were instructed to perform a nasal saline irrigation and apply a nasal spray in each nostril, and then informed to put a cotton ball in a one side of nostril, and the other side was kept to be empty as a control. Patients' subjective symptoms, patients' pain while receiving sinonasal cavity debridement, time required to perform debridement, and postoperative wound healing were evaluated.

Results

Although cotton ball packing resulted in less discomfort for postnasal drip, rhinorrhea, headache, and facial pain than no packing, there were no statistically significant differences between the groups. The cotton ball packing was associated with significantly less pain on while performing postoperative debridement, therefore less time was needed to perform debridement. The cotton ball packing appears to improve wound healing within the sinus cavities up to 1 month postoperatively.

Conclusion

The use of the cotton ball packing after ESS results in significantly less formation of crusts and adhesions, leading to decreasing pain and time during postoperative debridement and promoting faster wound healing.

http://bit.ly/2NbADJJ

Heat shock protein 27 inhibits HMGB1 translocation by regulating CBP acetyltransferase activity and ubiquitination

Publication date: April 2019

Source: Molecular Immunology, Volume 108

Author(s): Xiaowen Bi, Miao Xu, Jinfei Li, Ting Huang, Baolin Jiang, Lei Shen, Lan Luo, Shixiang Liu, Zhimin Yin

Abstract

Heat-shock protein 27 (Hsp27) is a member of the small heat shock protein family that has been reported to protect cells against pro-inflammatory stresses. High mobility group box 1 (HMGB1) is a proinflammatory cytokine associated with death from sepsis and other inflammatory diseases. After being acetylated by CREB-binding protein (CBP), the transcriptional adaptor and acetyltransferase, HMGB1 translocates from the nucleus to the cytoplasm. In the present study, we investigated the effects of Hsp27 on HMGB1 translocation from the nucleus to the cytoplasm in THP-1 cells. We found that Hsp27 phosphorylation decreased LPS-induced HMGB1 acetylation and translocation from the nucleus to the cytoplasm, as well as its release from THP-1 cells. The study further showed that cytosolic non-phosphorylated Hsp27 enhanced CBP ubiquitination and degradation in LPS-unstimulated cells, which suggested that Hsp27 maintained suitable CBP levels under normal physiological conditions. After LPS stimulation, Hsp27 was phosphorylated at serine residues 15/78 and translocated from the cytoplasm into the nucleus. Consequently, LPS stimulation increased CBP levels and promoted its translocation into the nucleus. In the nucleus, Hsp27 bound to CBP and suppressed CBP acetyltransferase activity and the subsequent CBP-dependent acetylation of HMGB1. Taken together, our data demonstrated that cytosolic non-phosphorylated Hsp27 enhanced the ubiquitin-mediated degradation of CBP, while phosphorylated Hsp27 inhibited CBP acetyltransferase activity in the nucleus. By regulating CBP, Hsp27 maintained cell homeostasis and inhibited excessive inflammatory response.

Graphical abstract

http://bit.ly/2BDM9Jx

Measuring anti-HLA antibody active concentration and affinity by surface plasmon resonance: Comparison with the luminex single antigen flow beads and T-cell flow cytometry crossmatch results

Publication date: April 2019

Source: Molecular Immunology, Volume 108

Author(s): Jonathan Visentin, Damien Le Leu, Arend Mulder, Frédéric Jambon, Laure Badier, Jar-How Lee, Gwendaline Guidicelli, Charlène Bouthemy, Mamy Ralazamahaleo, Frans Claas, Carmelo Di Primo, Jean-Luc Taupin

Abstract

Background

Although the Luminex single antigen flow beads (SAFB) and the flow cytometry crossmatch (FCXM) are the most sensitive assays used for anti-HLA antibodies characterization in transplant recipients, their semi-quantitative fluorescence read-out is not closely linked to graft outcome.

Methods

Surface plasmon resonance (SPR) was implemented to determine truly quantitative parameters of five human monoclonal anti-class I HLA antibodies (mAbs): first the active concentration and then the binding constants. The results were compared to those obtained with SAFB and T-cell FCXM (T-FCXM).

Results

The five mAbs displayed different rate and equilibrium constants for their cognate antigens. No correlation was evidenced between SAFB MFI or T-FCXM ratio and the binding parameters measured by SPR. Some mAbs amino acid substitutions within the epitope that influenced SAFB MFI resulted in affinity variations evidenced by SPR.

Conclusion

The SAFB MFI and T-FCXM ratio, both semi-quantitative parameters, only partially reflected the subtlety of the anti-HLA antibody/antigen interaction as it can be analyzed by SPR. Future clinical studies using SPR for anti-HLA antibodies characterization could bring novel insights into the understanding of HLA/anti-HLA interaction and therefore anti-HLA antibodies pathogenicity.

http://bit.ly/2BF4xBL

Cytokines use different intracellular mechanisms to upregulate the membrane expression of CX3CR1 in human monocytes

Publication date: April 2019

Source: Molecular Immunology, Volume 108

Author(s): Cecilia Analia Panek, Andrea Cecilia Bruballa, Gonzalo Ezequiel Pineda, Carlos De Brasi, Romina Jimena Fernández-Brando, María Pilar Mejías, María Victoria Ramos, Marina Sandra Palermo

Abstract

Membrane expression of fractalkine (CX₃CL1)-receptor (CX₃CR1) is relevant in monocytes (Mo) because CX₃CR1-CX₃CL1 interactions might participate on both, homeostatic and pathologic conditions. We have previously demonstrated that CX₃CR1 levels are decreased during culture and when Mo are differentiated into dendritic cells, but enhanced when differentiated into macrophages. Regarding soluble factors, lipopolysaccharide (LPS) accelerated the loss of CX₃CR1, while interleukin (IL)-10 and Interferon-gamma (IFN-γ) prevented it. However, the comprehensive knowledge about the intracellular pathways that underlay the level of CX₃CR1 expression in Mo is still incomplete.

In the current work, we studied the effect of anti-inflammatory cytokines (IL-4, IL-13, IL-10), alone or together with IFN- γ on CX₃CR1 expression. We found that only IL-10 and IFN-γ separately were able to prevent CX₃CR1 down-modulation during culture of human Mo. Besides, Mo incubated with IL-10 plus IFN-γ showed the highest CX₃CR1 expression by cell, suggesting cooperation between two different mechanism used by both cytokines. By studying intracellular mechanisms triggered by IL-10 and IFN-γ, we demonstrated that they specifically induced PI3K-dependent serine-phosphorylation of signal transducer and activator of transcription (STAT)3 or STAT1, respectively. Moreover, chemical inhibitors of STAT1 or STAT3 abrogated IFN-γ or IL-10 effects on CX₃CR1 expression. Strikingly, only IL-10 increased CX₃CR1 mRNA level, as consequence of augmenting mRNA stability. CX₃CR1 mRNA increase was PI3K-dependent, supporting the causal link between the action of IL-10 at the CX₃CR1 transcript and CX₃CR1 protein level on Mo. Thus, both cytokines up-regulate CX₃CR1 expression on human Mo by different intracellular mechanisms.

http://bit.ly/2TTJVwC

Risk factors and self‐perception for facial aging among Nepalese population

Summary

Background

Facial appearance has increasing importance to individuals and women, in particular. Till date, there are no reports of the extrinsic factors influencing skin aging in Nepalese population.

Objectives

To assess the risk factors and self‐perception for facial aging in Nepalese population.

Methods

A cross‐sectional study was conducted among 110 attendees aged 30‐70 years visiting the dermatology outpatient department of B.P. Koirala Institute of Health Sciences. An interview of the attendees was conducted to establish their perceived risk factors and age knowledge based on a questionnaire. The facial wrinkling was estimated by facial wrinkle scores using standardized visual assessment method by a dermatologist who was blinded with regard to all risk factors and age of the subjects.

Results

Around 50% of attendees were conscious about facial health, 53% perceived their facial age as their chronological age, 39% used some facial care products, and 68% were aware of the risk factors for facial aging. The major perceived risk factors were unhealthy eating habits, stress, less exercise, dehydration, diseased state and sleeping habits. A significant association was found between increasing age, place of birth in hills or mountains and sun exposure with high wrinkling score.

Conclusion

Facial skin wrinkles can be considered as a marker for intrinsic aging. Though the main factor responsible for extrinsic aging is ultraviolet radiation, identification of the factors may help in motivating the individuals for the adoption of a range of healthy behaviors for facial age in Nepalese population that may provide important public health benefits.

http://bit.ly/2DOWySC

Risk factors and self‐perception for facial aging among Nepalese population

Summary

Background

Facial appearance has increasing importance to individuals and women, in particular. Till date, there are no reports of the extrinsic factors influencing skin aging in Nepalese population.

Objectives

To assess the risk factors and self‐perception for facial aging in Nepalese population.

Methods

A cross‐sectional study was conducted among 110 attendees aged 30‐70 years visiting the dermatology outpatient department of B.P. Koirala Institute of Health Sciences. An interview of the attendees was conducted to establish their perceived risk factors and age knowledge based on a questionnaire. The facial wrinkling was estimated by facial wrinkle scores using standardized visual assessment method by a dermatologist who was blinded with regard to all risk factors and age of the subjects.

Results

Around 50% of attendees were conscious about facial health, 53% perceived their facial age as their chronological age, 39% used some facial care products, and 68% were aware of the risk factors for facial aging. The major perceived risk factors were unhealthy eating habits, stress, less exercise, dehydration, diseased state and sleeping habits. A significant association was found between increasing age, place of birth in hills or mountains and sun exposure with high wrinkling score.

Conclusion

Facial skin wrinkles can be considered as a marker for intrinsic aging. Though the main factor responsible for extrinsic aging is ultraviolet radiation, identification of the factors may help in motivating the individuals for the adoption of a range of healthy behaviors for facial age in Nepalese population that may provide important public health benefits.

http://bit.ly/2DOWySC

Salivary pH and flow rate in menopausal women

OBJECTIVE: This study aims at determining pH and Flow Rate (FR) of Unstimulated Whole Saliva (UWS) in a sample of 120 (60 menopausal women and 60 healthy fertile women with similar mean age); detecting the DMFT index (Decayed, Missing, Filled Teeth index) and evaluating any correlations between pH, FR, age and DMFT.

PATIENTS AND METHODS: Concerning the day before sample collection, patients were advised to keep a relaxed attitude and not to practice sports. They were also told to not eat or drink during the hour preceding sampling procedures. Saliva was collected via "spitting" method. Each sampling session started at 11:00 a.m., lasted for 5 minutes and used a pre-weighed, dry, deionized and sterile test tube. The procedure took place under controlled environmental temperature and humidity conditions (means 23.27°C; 60.08%). FR was evaluated via weighing technique and pH was measured with a portable pH-meter.

RESULTS: There was a minimal but significant pH difference (0.11; p<0.05) between menopausal women (6.75 ± 0.34) and fertile women (6.86 ± 0.24); and a FR difference (0.19; p<0.0001) between menopausal women (0.29 ± 0.17 mL/min) and fertile women (0.48 ± 0.19 mL/min). Correlation (R2) between pH and age was 0.0135 for fertile women and 0.0055 for menopausal women; while the correlation between FR and age was 0.0673 for fertile women and 0.139 for menopausal women. Mean DMFT was 11.93 ± 7.14 in menopausal women and 12.23 ± 6.37 in fertile women.

CONCLUSIONS: We observed a minimal decrease in pH and a decrease in FR in menopausal women. Further studies will be needed to investigate the possible role of other environmental and individual variables in the determination of such values.

L'articolo Salivary pH and flow rate in menopausal women sembra essere il primo su European Review.

http://bit.ly/2SRM7HO