Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation

xlomafota13 shared this article with you from Inoreader Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2127-2134. doi: 10.21873/invivo.12482. ABSTRACT BACKGROUND/AIM: Mesenchymal stem cells (MSCs) have been widely used for yielding neurons in culture to study nervous system pathologies and develop regenerative approaches. In this study, cellular rearrangements of human MSCs related to the expression of the fibronectin common receptor integrin α5β1 and its cell surface localization during neuronal differentiation, were examined. MATERIALS AND METHODS: Proliferation kinetics of neuronal induced hMSCs (hMd-Neurons) were quantified by BrdU assay, and hMd-Neurons were immunostained for neuronal marker expression. Additionally, cDNA and protein samples were collected at different time points for integrin α5β1 expression analysis. RESULTS: Endogenous integrin α5β1 expression was significantly upregulated by day 6 and maintained until day 12. Cell surface localization of α5β1 integrin was increase d by day 6; the integrin was internalized into the cytosol by day 12. CONCLUSION: Integrin dynamics around day 6 of differentiation might be involved in neuronal differentiation and maturation or specification of hMd-Neurons. PMID:34182488 | DOI:10.21873/invivo.12482 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Clinical Significance of Composition Changes in T-cell Subpopulations After Chemotherapy in Patients With Gastric Cancer

xlomafota13 shared this article with you from Inoreader Clinical Significance of Composition Changes in T-cell Subpopulations After Chemotherapy in Patients With Gastric Cancer Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2417-2424. doi: 10.21873/invivo.12519. ABSTRACT BACKGROUND/AIM: New therapeutic agents and prognostic biomarkers for gastric cancer are needed. We analyzed the composition of peripheral blood T-cell subpopulations in response to chemotherapy in patients with gastric cancer. PATIENTS AND METHODS: Peripheral blood samples were collected from patients diagnosed with gastric cancer before and after chemotherapy (FOLFOX; oxaliplatin, 5-fluorouracil, and leucovorin). Peripheral blood mononuclear cells were isolated. Patients were divided into responder (n=5) and non-responder groups (n=2) based on their chemotherapy outcomes. RESULTS: Non-responders showed lower numbers of CD4+/total cells and CD8+/total cells after chemotherapy compared to the responder group, but the difference was not significant (p=0.905, p=0.095). Naïve T, central memory T, effector memory T and effector T-cell counts differed in both groups after chemotherapy. CONCLUSION: Changes in peripheral T-cell subpopulations after chemotherapy were confirmed in patients with gastric cancer, which may be a prognostic predictor and development of therapeutic agents. PMID:34182525 | DOI:10.21873/invivo.12519 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Ureteric Bud-derivatives in Wilms Tumor and Nephrogenic Rest

xlomafota13 shared this article with you from Inoreader Ureteric Bud-derivatives in Wilms Tumor and Nephrogenic Rest Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2159-2162. doi: 10.21873/invivo.12486. ABSTRACT BACKGROUND/AIM: Recent studies suggest that not only the nephrogenic blastema but also the ureteric bud is involved in oncogenesis of Wilms' tumor (WT). However, the occurrence of ureteric bud (UB) derivatives in nephrogenic rest is not yet known. The aim of our study was to find UB derivatives in WT. MATERIALS AND METHODS: Keratin 17 (KRT17) is expressed exclusively in UB in foetal kidneys. In this study KRT17 immunohistochemistry was used to detect UB-derivatives in 21 triphasic, 2 stromal and 3 epithelial predominant WTs and 9 nephrogenic rests. RESULTS: We have detected KRT17 positive tubular structures resembling UB in 3 of 9 nephrogenic rests and 15 of 26 WTs. CONCLUSION: Not only the metanephric blastema but also the UB is involved in the histogenesis of nephrogenic rest and WT. PMID:34182492 | DOI:10.21873/invivo.12486 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Expression of LAT1 and 4F2hc in Gastroenteropancreatic Neuroendocrine Neoplasms

xlomafota13 shared this article with you from Inoreader Expression of LAT1 and 4F2hc in Gastroenteropancreatic Neuroendocrine Neoplasms Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2425-2432. doi: 10.21873/invivo.12520. ABSTRACT BACKGROUND/AIM: Little is known about the expression of L-type amino acid transporter 1 (LAT1) and 4F2hc in gastroenteropancreatic-neuroendocrine neoplasms (GEP-NENs). Hence, we conducted a study to verify the clinicopathological significance of LAT1 and 4F2hc. PATIENTS AND METHODS: Tissues from 126 patients with GEP-NENs were collected between August 2007 and September 2019 at our institution. We evaluated LAT1 and 4F2hc expression by immunohistochemistry, and examined their clinical significance. RESULTS: No statistically significant associations were observed between LAT1 expression and the different NENs. Expression of 4F2hc was significantly different between neuroendocrine tumour (NET)-G1, NET-G2, and NET-G3 (p=0.029), and was significantly associated with vascular invasion (p=0.044) and the Ki-67 index (p=0.042). CONCLUSION: No associat ion between LAT1 expression and malignant features in GEP-NENs was observed. However, an association between 4F2hc expression and the potential of malignancy in GEP-NENs was evident. PMID:34182526 | DOI:10.21873/invivo.12520 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Midterm Results Following Minimally Invasive Distal Chevron Osteotomy: Comparison With the Minimally Invasive Reverdin-Isham Osteotomy by Means of Meta-analysis

xlomafota13 shared this article with you from Inoreader Midterm Results Following Minimally Invasive Distal Chevron Osteotomy: Comparison With the Minimally Invasive Reverdin-Isham Osteotomy by Means of Meta-analysis Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2187-2196. doi: 10.21873/invivo.12490. ABSTRACT BACKGROUND/AIM: To date, multiple different surgical techniques have been established for hallux valgus surgery, with each technique having its unique advantages and limitations. The open distal chevron osteotomy is widely accepted, but increasing patient demands have led several minimally invasive (MIS) techniques to be described in recent years. The aim of this study was to compare outcomes after minimally invasive (MIS) distal chevron osteotomy and the minimally invasive Reverdin-Isham method. PATIENTS AND METHODS: We assessed clinical and radiographic outcomes after MIS chevron osteotomy in 57 feet of 49 consecutive patients with a mean follow-up of 58.9 (range=39.0-85.4) months. Outcomes after MIS Reverdin-Isham osteotomy were analyzed by means of a systematic literature review with a minimum follow-up of 6 months. RESULTS: Radiographic outcome s were significantly better in the MIS chevron cohort for intermetatarsal angle (p<0.001), hallux valgus angle and distal metacarpal articular angle (p<0.05). Concerning clinical outcomes, both methods provided comparable improvement. CONCLUSION: MIS distal chevron osteotomy in mild to moderate hallux valgus deformity correction results in superior radiographic outcomes compared to the MIS Reverdin-Isham osteotomy. Sufficient correction of IMA cannot be achieved with the MIS Reverdin-Isham osteotomy. PMID:34182496 | DOI:10.21873/invivo.12490 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Time Dependence of Intra-fractional Motion in Spinal Stereotactic Body Radiotherapy

xlomafota13 shared this article with you from Inoreader Time Dependence of Intra-fractional Motion in Spinal Stereotactic Body Radiotherapy Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2433-2437. doi: 10.21873/invivo.12521. ABSTRACT BACKGROUND/AIM: Positional uncertainty in spinal stereotactic body radiotherapy (SBRT) may cause fatal error, therefore, we investigated the intra-fractional spinal motion during SBRT and its time dependency. PATIENTS AND METHODS: Thirty-one patients who received SBRT using CyberKnife were enrolled in the study. 2D kV X-ray spine images in two directions were taken before and during treatment. Image acquisition intervals during treatment were set at 35-60 sec. Automatic image matchings were performed between the reference digital reconstructed radiography (DRR) and live images, and the spinal position displacements were logged in six translational and rotational directions. If the displacements exceeded 2 mm or 1 degree, the treatment beam delivery was interrupted and the patient position was corrected by moving couch, and the couch adjustments were also logged. Based on the information, the time-dependent accumulated translational and rotational displacements without any couch adjustments were calculated. RESULTS: Spinal position displacements in all translational and rotational directions were correlated with elapsed treatment time. Especially, Right-Left displacements of >1 mm and >2 mm were observed at 4-6 and 8-10 min after treatment initiation, respectively. Rotational displacements in the Yaw direction >1° were observed at 10-15 min after treatment initiation. CONCLUSION: The translational and rotational displacements systematically increased with elapsed treatment time. It is suggested that the spine position should be checked at least every 4-6 min or the treatment time should be limited within 4-6 minutes to ensure the irradiation accuracy within the millimeter or submillimeter range. PMID:34182527 | DOI:10.21873/invivo.12521 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Can Dynamic Contrast-enhanced MRI Contribute to Improved Assessment of Rectosigmoid Involvement in Deep Infiltrating Endometriosis?

xlomafota13 shared this article with you from Inoreader Can Dynamic Contrast-enhanced MRI Contribute to Improved Assessment of Rectosigmoid Involvement in Deep Infiltrating Endometriosis? Via In vivo (Athens, Greece) In Vivo. 2021 Jul-Aug;35(4):2217-2226. doi: 10.21873/invivo.12494. ABSTRACT BACKGROUND/AIM: To determine whether a prototypical compressed-sensing volume-interpolated breath-hold (csVIBE) provides diagnostic value in detecting rectosigmoid infiltration in deep infiltrating endometriosis (DIE). PATIENTS AND METHODS: csVIBE was employed in 151 women undergoing pelvic magnetic resonance imaging, of whom 43 had undergone surgery for suspected endometriosis. The accuracy of T2-weighted BLADE and BLADE/csVIBE, additional diagnostic value of csVIBE, and diagnostic confidence were rated by two readers. Additionally, the presence of the "mushroom cap sign" was assessed on BLADE and csVIBE. RESULTS: The diagnostic accuracy, sensitivity, and specificity of BLADE and BLADE/csVIBE were not significantly different between Readers A and B. For both readers, the confidence in the diagnosis increased with csVIBE, but this increase in the od ds ratio was not significant for both readers. Both readers preferred csVIBE over BLADE with regard to detection of the "mushroom cap sign." CONCLUSION: csVIBE may provide a diagnostic benefit for surgical strategy selection through better delineation of the "mushroom cap sign." PMID:34182500 | DOI:10.21873/invivo.12494 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.