Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com

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Παρασκευή 24 Μαΐου 2019

Palaeogeography, Palaeoclimatology, Palaeoecology

Astronomical constraints on deposition of the Middle Triassic Chang 7 lacustrine shales in the Ordos Basin, Central China

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Rui Zhang, Zhijun Jin, Quanyou Liu, Peng Li, Zhenkai Huang, Juye Shi, Yunjin Ge, Kefeng Du

Abstract

The Middle Triassic was a key period that witnessed the evolution of Earth system processes and the commencement of a terrestrial lake in the Ordos Basin, Central China. A high-precision stratigraphic framework is the key to understanding the nature and pattern of critical geological events. Detailed time series analyses of magnetic susceptibility (MS) data were performed on the deep lacustrine shale-dominated Chang 7 Member of the Yanchang Formation from the Y1011 well core. The results reveal well documented cyclic variations with wavelengths of 5.37 m, 1.39–1.78 m, 0.48 m, and 0.24–0.30 m, which are driven by long-eccentricity, short-eccentricity, obliquity, and precession in the Middle Triassic. The stable 405-kyr tuned floating astrochronological time scale (FATS) reveals that the depositional duration of the Chang 7 Member is approximately 5 Myr, and the sedimentation rates range from 0.90 cm/kyr to 1.69 cm/kyr. In particular, the lower part of the Chang 7 Member is characterized by an organic-rich, black shale, called "Zhangjiatan Shale", whose depositional duration can be estimated at about 1.7 Myr. Along with the published biostratigraphic divisions and UPb age constraints, our FATS further confirms that the Chang 7 Member mainly developed in the Ladinian Stage, and that the upper part of the Chang 7 spanned the Middle/Late Triassic boundary. The duration of the Chang 7 deposition suggests a temporal and genetic linkage between the responses of the Chang 7 shales in the Ordos Basin and the Indosinian Orogeny in the Qinling orogenic belt. The Ladinian Stage of the Middle Triassic probably witnessed a dramatic shift in the evolution of the regional geodynamic system.



Are light-dark coupled laminae in lacustrine shale seasonally controlled? A case study using astronomical tuning from 42.2 to 45.4 Ma in the Dongying Depression, Bohai Bay Basin, eastern China

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Ke Zhao, Xuebin Du, Yongchao Lu, Shipeng Xiong, Yong Wang

Abstract

As a typical sedimentary structure in fine-grained rocks, laminae are widely distributed in shales and mudstones in sedimentary basins. The Shahejie Formation (42.2–45.4 Ma) formed during the Eocene of the Paleogene in the Dongying Depression of China is recognized as a typical area to study lacustrine shale. According to the composition of different lamina, four types of couplets are identified, including carbonate-clay couplets, carbonate-organic couplets, clay-organic couplets and carbonate-clay-organic triplets. All couplets are composed of light and dark layers. A combination of core images, microscopic observations, mineral compositions, geochemical data, carbon and oxygen isotopes, and strontium isotopes verifies that the laminae are primarily developed in a saline and anoxic, or even euxinic environment, with a high organic matter (OM) flux. Through astronomical cycle analysis of the natural gamma ray (GR) curve from sample site well NY1, the data suggest that the lacustrine shale laminae are formed annually with an average duration of 1.34 yr. The formation of laminae is affected by the season, which is closely related to the seasonal growth and death of algae. During the period of algae growth, a large number of light-colored carbonate laminae are deposited, whereas when the algae dies, organic matter accumulates at the bottom of lakes and forms dark organic-rich laminae. The interbedded and lenticular laminae, which are subdivided by layering characteristics, are primarily affected by diagenesis in postdepositional processes. Study of the laminae is helpful in understanding the formation process of lacustrine shale, and it provides invaluable sources of information for paleoclimate reconstruction.



δ13C stratigraphy of a Turinian-Chatfieldian (Upper Ordovician) foreland succession, Ottawa Embayment (central Canada): resolving local and inter-regional isotope excursions in a tectonically active basin

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Nkechi E. Oruche, George R. Dix, Sean Gazdewich

Abstract

Positive δ13Ccarb excursions are correlated through an upper Turinian to lower Chatfieldian carbonate-platform succession along the axis of the Ottawa Embayment and into outliers of the northern Ottawa-Bonnechere graben in central Canada. Successive Turinian excursions (E1 and E2) are lithostratigraphically constrained by erosional surfaces and hosted within the Watertown and overlying L'Orignal formations, respectively, the latter coeval with the Selby Formation in the adjacent northern Appalachian Basin. The excursions coincide with periods of regional transgression, but geographic patterns of 13C depletion versus enrichment coincide with structurally defined areas of stratigraphically condensed and preferentially thickened formation successions, respectively. Differential subsidence is interpreted to have created bathymetric variation resulting in intrabasinal restriction of seawater exchange between these areas, with preferential Corg recycling with stratigraphic condensation. By early Chatfieldian time, segmentation of the once regional carbonate platform (L'Orignal Formation) produced a regional mosaic of low-energy muddy carbonate banks (Rockland Formation) and a deeper water platform (lower Hull Formation) settings subject to fluctuating high to low energy current flow. Excursion E3 occurs in both successions, but 13C enrichment is associated only with the bank-top muddy facies. This may identify preferential photosynthetic drawdown of 12C across the bank tops due to limited seawater exchange across the bank-deeper platform boundaries. Excursions E1 to E3, and a younger excursion (E4) in the Hull Formation, are correlated with varying confidence with excursions across southern Laurentia, excursion E3 being the local expression of the Guttenberg δ13C excursion. Our study supports local modulation of regional, if not global, δ13C excursions arising from structurally controlled changes in oceanography and productivity.



Addressing the effects of sampling on ecometric-based paleoenvironmental reconstructions

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): J. Tyler Faith, Andrew Du, John Rowan

Abstract

Ecometric analysis involves the examination of quantifiable functional traits across the taxa in a biotic community. Well-documented relationships between certain functional traits and environmental gradients in the present provide the empirical framework for a large body of research that uses ecometrics to reconstruct environments in the fossil record. In current applications of the technique, the taxa present in a fossil assemblage are summarized using the mean value of an environmentally informative trait. This study explores some of the quantitative pitfalls inherent to this approach. Through analysis of dental crown height—a trait that is widely used to infer paleo-precipitation—of Late Pleistocene ungulate assemblages from the Lake Victoria Basin in western Kenya, we illustrate how ecometric means vary as a function of sample size. Sampling artifacts have the potential to bias ecometric means, and the environmental inferences derived from them, whenever there is a non-random distribution of traits across the species abundance distribution (e.g., if abundant taxa have different traits than rare taxa). This sampling issue also means that the degree of analytical precision implied by quantitative paleoenvironmental reconstructions (e.g., annual precipitation at time X was 500 mm/yr) derived from ecometrics may be unwarranted. We recommend that analytical approaches be modified to circumvent these problems and explore three potential solutions: (1) specimen-based rarefaction, (2) coverage-based rarefaction, and (3) weighting ecometric means by taxonomic abundances. Of these, only the latter is robust to variation in sampling effort. Because abundance data are not always available and are potentially unreliable, we outline alternative approaches that could be implemented to contend with sample bias.



Evidence of SPICE-related anoxia on the Laurentian passive margin: Paired δ13C and trace element chemostratigraphy of the upper Conasauga Group, Central Appalachian Basin

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Justin E. Mackey, Brian W. Stewart

Abstract

This study reports data from the Upper Cambrian Conasauga Group and overlying Copper Ridge Formation of the Central Appalachian region, eastern U.S.A. Geochemical, isotopic and petrographic analysis of core material was carried out to constrain the extent of marine anoxia and to characterize sediment fluxes on the carbonate platform and continental shelf of Laurentia contemporaneous with the Steptoean Positive Carbon Isotope Excursion (SPICE), a Late Cambrian global marine anoxic event. Carbonate rocks (primarily dolostone) record a positive δ13Ccarb excursion starting in the middle Nolichucky Formation, reaching its peak (+4.3‰) at the boundary between the Maynardville and Copper Ridge formations. Strontium isotope ratios in the dolostone units are only slightly offset from the expected Cambrian seawater values, suggesting minimal post-diagenetic disturbance of isotopic and trace element systematics. Selective leaching of carbonate units reveal upward decreasing trends in dissolved redox-sensitive trace metals (e.g., U, Ni, V) indicative of drawdown from regional and global marine anoxia during the peak of the Late Cambrian SPICE event.



Palaeoecological implications of an Upper Cretaceous tetrapod burrow (Bauru Basin; Peirópolis, Minas Gerais, Brazil)

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Agustín G. Martinelli, Giorgio Basilici, Lucas E. Fiorelli, Carolina Klock, Joachim Karfunkel, Ariela Costa Diniz, Marcus V.T. Soares, André Marconato, João Ismael da Silva, Luiz Carlos B. Ribeiro, Thiago S. Marinho

Abstract

We describe a globally rare example of a tetrapod burrow from the Upper Cretaceous Bauru Group (Bauru Basin) from Peirópolis, Minas Gerais State, Brazil. The sedimentary succession containing the burrow includes a rich vertebrate assemblage comprising fish, podocnemid turtles, mesoeucrocodylians, saurischian dinosaurs, among others. The burrow is composed of an oblique tunnel (~30°), oval in cross-section, with a horizontal and sub-oval terminal chamber; it is 1.3 m long from the midpoint of its inferred entrance to the midpoint of the bottom of the chamber. It occurs in the upper portion of a sandstone succession, interpreted as a braided channel deposit, and the burrow-fill comprises medium-grained sandstone with mudstone intraclasts derived from fluvial floodplain facies. it is overlain by other fluvial channel deposits. Analyses suggest that the burrow was dug after the filling of the braided channel and during the pedogenesis of its exposed upper surface. Based on burrow morphology and size, the most plausible producer of this burrow is a notosuchian mesoeucrocodylian, such as small to mid-sized notosuchians (e.g., sphagesaurids). The Bauru Group has an extensive fossil record of notosuchians with disparate morphologies, and it is noteworthy that the small-sized notosuchian Labidiosuchus amicum comes from the same unit as the burrow. Moreover, arid to semi-arid conditions have been inferred for fossil-bearing rocks of this unit, and as such the data here presented add to our palaeoecological knowledge of Cretaceous mesoeucrocodylians in Gondwana. Moreover, it constitutes a new Cretaceous record of a tetrapod burrow during a period when such ichnofossils are globally rare.



Dental histology of late Miocene hipparionins compared with extant Equus, and its implications for Equidae life history

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Guillem Orlandi-Oliveras, Carmen Nacarino-Meneses, Meike Köhler

Abstract

Hipparionins were a dominant element of the late Miocene faunas of Europe; however, their biology and ecology remain incompletely understood. In this paper, we explore the pace of life history of different-sized hipparionin horses, using dental histology, and compare it with extant equids. In doing so we consider (i) the size diversity of hipparionins, (ii) their generally smaller size compared to extant equids, and (iii) the allometric coupling between size and life history. In particular, we reconstruct the dental growth in lower first/second molars and in later-formed lower third molars for three hipparionin taxa: two dwarf species (Hipparion periafricanumand H. gromovae), and a larger species (H. concudense). We also analyze dental growth in an extant zebra (Equus quagga) for comparative purposes. Our results reveal that, within each species, there are differences in enamel growth parameters between the first/second molars and third molars. These results illustrate the differences in the developmental timing of these teeth and the existence of a relationship between dental growth parameters with somatic growth. We also find that hipparionin teeth grow at slower rates and tend to erupt later in time than in extant Equus. Dwarf hipparionins, moreover, exhibit lower enamel extension rates than the larger species, but similar formation and eruption times. Considering the link between dental development and life history, these results suggest a slower pace of growth of selected hipparionins compared to extant equids, and a further slower life history than expected for their size in the two dwarf forms.



Cyclonic activity over northeastern Africa at 8.5–6.7 cal kyr B.P., based on lacustrine records in the Faiyum Oasis, Egypt

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): Leszek Marks, Fabian Welc, Krystyna Milecka, Abdelfattah Zalat, Zhongyuan Chen, Aleksandra Majecka, Jerzy Nitychoruk, Alaa Salem, Qianli Sun, Marcin Szymanek, Izabela Gałecka, Anna Tołoczko-Pasek

Abstract

During African Humid Period in the Holocene when the summer Intertropical Convergence Zone migrated to its northernmost position, the Qarun Lake in the Faiyum Oasis in Egypt was fed with regular inflows from the Nile River and rainfall brought by the Mediterranean winter circulation. Finely-laminated lake sediments, dated at 8.5–6.7 cal kyr B.P., were examined in terms of lithology, geochemistry, microfossils (diatom, pollen) and magnetic susceptibility. Based on the inferred geographical derivation of pollen, the environmental affiliation of diatom taxa and geochemistry of lake sediments, wind trajectories were distinguished, related to two main atmospheric circulation phases. During the earlier phase (8.50–7.83 cal kyr B.P.) there were northwestern wind trajectories followed by southern ones and during the later phase (7.83–6.70 cal kyr B.P.), the northern winds were followed by northwestern and southern ones. Northwestern and northern winds brought winter rainfall and caused water turbulence in the lake, and the southern winds were associated with regional aridification. This scenario of atmospheric circulation in northeastern Africa extends significantly our understanding of key modes of climatic variability and wind trajectories in the Early to Middle Holocene (Greenlandian to Northgrippian) transition.



How quick was marine recovery after the end-Triassic mass extinction and what role did anoxia play?

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): J.W. Atkinson, P.B. Wignall

Abstract

Oxygen restricted conditions were widespread in European shelf seas after the end-Triassic mass extinction event and they are reported to have hindered the recovery of marine benthos. Here we reconstruct the redox history of the Early Jurassic Blue Lias Formation of southwest Britain using pyrite framboid size analysis and compare this with the recovery of bivalves based on field and museum collections. Results suggest widespread dysoxia punctuated by periods of anoxia in the region, with the latter developing frequently in deeper water settings. Despite these harsh conditions, initial benthic recovery occurred rapidly in the British Jurassic, especially in shallowest settings, and shows no relationship with the intensity of dysoxia. A stable diversity was reached by the first recognised ammonite zone after the end-Triassic mass extinction. This contrasts with the deeper-water, more oxygen-poor sections where the diversity increase was still continuing in the earliest Sinemurian Stage, considerably longer than previously reported. Similar recovery rates are seen amongst other groups (brachiopods and ammonites). Oxygen-poor conditions have been suggested to delay recovery after the Permo-Triassic mass extinction, but this is not the case after the end-Triassic crisis. We suggest that this was because the European dysoxia was only a regional phenomenon and there were plenty of well-ventilated regions available to allow an untrammelled bounce back.



Ecophenotypic shape variability within Astarte (Class: Bivalvia) from the Pliocene of the Atlantic Coastal Plain, U.S.A.: A study using geometric morphometrics

Publication date: 15 August 2019

Source: Palaeogeography, Palaeoclimatology, Palaeoecology, Volume 528

Author(s): R. Philip Roberson, Michelle M. Casey

Abstract

The Atlantic Coastal Plain has long been recognized as a natural laboratory useful for testing hypotheses surrounding the environmental and ecological effects on marine fauna. To conduct these studies in a rigorous manner, a reliable taxonomy must be established for the organisms within this physiographic province. In this study, we focus on the bivalve genus Astarte, which is commonly found in this region, and has many formally recognized species. We test between two competing hypotheses for the unusually high species richness of this genus: 1) taxonomy – the presence of discrete morphotypes represent valid species within this genus; and, 2) ecology – the many recognized 'species' in this genus in fact represent ecophenotypic variants of a smaller number of species, and thus richness is inflated. We test between these two hypotheses using a geometric morphometric analysis of landmarks placed on 646 shells, representing six different taxa from the Pliocene of the Atlantic Coastal Plain. We performed Procrustes transformation and Principle Components Analysis (PCA) on landmark data, allometric residuals, and outline harmonics to fully understand the variability of morphologies present. All PCA results show large amounts of overlap between all species. It is likely some of these species are valid taxonomic groups within the genus but should be classified as sub-species instead of separate species. These results provide strong support for our 'ecology' hypothesis, and suggests the genus Astarte needs revision. Future studies incorporating detailed sedimentological characteristics or ecological information (e.g., drilling frequency) will further elucidate palaeoecological and palaeoenvironmental drivers of ecophenotypic variation present in this genus. Further, the accurate identification of fossil species has important implications for our ability to reconstruct biodiversity trends in relation to the emergence of the Central American Isthmus and subsequent changes in ocean circulation.



Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
6948891480

The Full Strawberry Moon marks strawberry harvesting season


Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
6948891480

Ophthalmology

Do we need India-specific retinopathy of prematurity screening guidelines?
Santosh G Honavar

Indian Journal of Ophthalmology 2019 67(6):711-716



The changing scenario of retinopathy of prematurity in middle and low income countries: Unique solutions for unique problems
Anand Vinekar, Mangat Dogra, Raj Vardhan Azad, Clare Gilbert, Lingam Gopal, Michael Trese

Indian Journal of Ophthalmology 2019 67(6):717-719



Children - Not just small adults!
Lingam Gopal

Indian Journal of Ophthalmology 2019 67(6):720-721



The Indian Retinopathy of Prematurity (iROP) society: Challenges ahead
Anand Vinekar, Raj Vardhan Azad

Indian Journal of Ophthalmology 2019 67(6):722-722



Imaging the pediatric retina – Where are we headed?
Sabyasachi Sengupta

Indian Journal of Ophthalmology 2019 67(6):723-724



Retinal elevation in an infant
Antonio N Yaghy, Lauren A Dalvin, Carol L Shields

Indian Journal of Ophthalmology 2019 67(6):725-725



India to gear up to the challenge of "third epidemic" of retinopathy of prematurity in the world
P Sai Kiranmayee, Viswanath Kalluri

Indian Journal of Ophthalmology 2019 67(6):726-731

Many of the causes of childhood blindness are avoidable, being either preventable or treatable. Retinopathy of prematurity (ROP) remains one of the most preventable causes of childhood blindness worldwide. Currently, India is facing the third epidemic of ROP. In India, the health system involving the mother and child health services needs to be strengthened with a policy to cover the existing inadequacies in neonatal care and implementation of program covering newborn, especially premature. The access, availability, and affordability of services related to the care of premature babies need strengthening in India. ROP-trained ophthalmologists and neonatal care pediatricians and a professional togetherness is a big issue. Inadequacies in awareness of ROP among the parents, health care workers, counsellors add up to the problem. Community-based health workers such as Accredited Social Health Activist are a good dependable force in India and are needed to be trained in awareness and establishing a proper identification for prompt referral. ROP prevention needs a multidisciplinary team approach. ROP management stands as a good example of all the strategies for prevention, which includes primary prevention (improving obstetric and neonatal care), secondary prevention (screening and treatment programs), and tertiary prevention (treating complications and rehabilitation to reduce disability). Given its demographic and cultural diversity, India faces numerous challenges, with significant rural–urban, poor–rich, gender, socioeconomic, and regional differences. So, we need to gear up to face the present challenge of the third epidemic of ROP and prevent ROP-related childhood blindness as it is the need of the hour. 


Pediatric vitreous hemorrhage: A narrative review
Anmol U Naik, Ekta Rishi, Pukhraj Rishi

Indian Journal of Ophthalmology 2019 67(6):732-739

Vitreous hemorrhage is one of the most common causes of sudden, painless loss of vision in adults. This is probably one of the reasons why it has been extensively studied and reported in literature. However, the same cannot be said when it comes to vitreous hemorrhage in the pediatric age group. The causes of vitreous hemorrhage in children tend to differ from those of adults. Not much data exist regarding their presentation and management. In addition to trauma, certain spontaneous causes such as pediatric tumors and congenital conditions assume importance while considering the differential diagnosis of vitreous hemorrhage in the pediatric age group. However, it is natural that the treating ophthalmologist is faced with challenges when a child presents with vitreous hemorrhage. In this narrative review, we have attempted to analyze the retrospective observational studies regarding pediatric vitreous hemorrhage reported in English literature till date. The article sheds some light on the prevailing epidemiology, management strategies employed and the visual outcome among different regions of the world. 


Intra-arterial chemotherapy in retinoblastoma – A paradigm change
Fairooz P Manjandavida, Christina Stathopoulos, Jing Zhang, Santhosh G Honavar, Carol L Shields

Indian Journal of Ophthalmology 2019 67(6):740-754

Intra-arterial chemotherapy (IAC), also known as superselective ophthalmic artery chemotherapy or chemosurgery, is currently widely accepted as one of the primary treatment modalities for intraocular retinoblastoma worldwide. Following the introduction of the technique in 1998, IAC has evolved over the past decades to be safer and more effective. Accumulated evidence shows that IAC is more effective in providing eye salvage in group D and E retinoblastoma as compared to conventional systemic intravenous chemotherapy (IVC). In contrast to IVC, IAC has the added benefits of reduced overall treatment duration and minimal systemic toxicity. This review provides a comprehensive update on the history, technique, indications, contraindications, and outcome of IAC. We have also identified the strengths, weaknesses, opportunities and threats (SWOT analysis) of the technique in this review. 


Intraocular medulloepithelioma – A review of clinical features, DICER 1 mutation, and management
Sameeksha H Tadepalli, Carol L Shields, Jerry A Shields, Santosh G Honavar

Indian Journal of Ophthalmology 2019 67(6):755-762

Intraocular medulloepithelioma is a nonhereditary neoplasm of childhood arising from primitive medullary epithelium. It most often involves the ciliary body. Most patients present between 2 and 10 years of age with loss of vision, pain, leucocoria, or conjunctival congestion. The mass appears as a grey-white ciliary body lesion with intratumoral cysts. Presence of a neoplastic cyclitic membrane with extension to retrolental region is characteristic. Secondary manifestations like cataract and neovascular glaucoma may be present in up to 50% and 60% patients, respectively. These could be the first signs for which, unfortunately, about 50% patients undergo surgery before recognition of the hidden tumor. Systemic correlation with pleuropulmonary blastoma (DICER1 gene) has been documented in 5% cases. Histopathology shows primitive neuroepithelial cells arranged as cords closely resembling the primitive retina. Histopathologically, the tumor is classified as teratoid (containing heteroplastic elements) and nonteratoid (containing medullary epithelial elements), each of which are further subclassified as benign or malignant. Retinoblastoma-like and sarcoma-like areas may be seen within the tissue. The treatment modality depends on tumor size and extent of invasion. For small localized tumors (≤3-4 clock hours), conservative treatments with cryotherapy, plaque radiotherapy, or partial lamellar sclerouvectomy (PLSU) have been used. Plaque brachytherapy is generally preferred for best tumor control. Advanced and extensive tumors require enucleation. Rare use of intra-arterial and intravitreal chemotherapy has been employed. Systemic prognosis is favorable, but those with extraocular extension and orbital involvement show risk for local recurrence and metastatic disease, which can lead to death. 


Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
6948891480

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry

The Effect of The Neuroprotector Isatin on Complex Formation of Beta-Amyloid Peptide Fragments with Some Intracellular Proteins

Abstract

Amyloid-β peptide (1−42) (Aβ1-42) is a key player in the development and progression of Alzheimer's disease (AD) and related pathologies, determined by formation of protein aggregates in the central nervous system. Aβ1-42 binding to crucial intracellular targets (and their subsequent inactivation) obviously represents one of the earliest events preceding extracellular pathogenic oligomerization/aggregation of Aβ1-42. It is reasonable to expect that dissociation of the Aβ1-42 complexes with intracellular proteins by means of inhibitors followed by subsequent degradation of Aβ1-42 would not only protect critically important proteins but also prevent intracellular accumulation of Аβ1-42. The aim of this study was to investigate the effect of the neuroprotector isatin (100 µM) on interaction of known Aβ-binding proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and pyruvate kinase, with Aβ1-42 and its fragments (Aβ1-28, Aβ12-28, Aβ25-35). Aβ1-42 and its fragments (Aβ1-28, Aβ12-28, Aβ25-35) immobilized on the Biacore optical biosensor chip interacted with GAPDH and pyruvate kinase. The lowest and basically equal Kd values were determined for GAPDH and pyruvate kinase complexes with immobilized Aβ1-42 and Aβ25-35. The presence of 100 µM isatin caused a significant (more than fivefold) increase in the Kd values for GAPDH complexes with all Aβ peptides except Aβ1-28. In contrast to GAPDH isatin increased dissociation of pyruvate kinase complexes only with Aβ1-42 (causing a 30-fold increase in Kd) and to a lesser extent with Aβ12-28 and Aβ25-35 (a 10-fold increase in the Kd value). It should be noted that in the presence of isatin the Kd values for GAPDH and pyruvate kinase complexes with all Aβ studied were in a narrower concentration range (10–7 M–10−6 M) than in the absence of this neuroprotector (10–8 M–10–6 M). Data obtained suggest existence of principal possibility of (pharmacological) protection of crucial intracellular targets against both Aβ1-42, and its aggressive truncated peptides (Aβ25-35).



Chronic Alcoholism Influences the mRNA Level of the Orexin Receptor Type 1 (OX1R) in Emotiogenic Structures of the Rat Brain

Abstract

Orexin and its receptors are involved in mechanisms of pathological craving to alcohol. This study has shown that the mRNA level of the orexin receptor type 1 (OX1R) significantly decreased in the prefrontal cortex in the group of chronically alcoholized (for 6 months) rats compared with intact control. The same changes in the OX1R gene expression were observed on day 1 and day 7 of alcohol withdrawal after chronic alcoholization. On the contrary, in the hippocampus, the OX1R mRNA level increased on day 1 and day 7 of alcohol withdrawal. In the ventral tegmental area, the OX1R mRNA level remained unchanged on both day 1 and day 7 of alcohol withdrawal compared with the groups of chronic alcoholization and intact control. This suggests involvement of the prefrontal cortex and hippocampus in mechanisms mediating chronic alcohol intoxication.



Comparative Analysis of Gene Expression in Vascular Cells of Patients with Advanced Atherosclerosis

Abstract

A comparative analysis of gene expression profiles of carotid atherosclerotic plaques and intact internal thoracic arteries of patients with advanced atherosclerosis was performed by using the Human-12 BeadChip Microarray (Illumina, USA). The most down-regulated genes in the carotid atherosclerotic plaques were APOD, FABP4CIDEC, and FOSB, in contrast, up-regulated gene was SPP1 (|FC| > 64; pFDR < 0.05). The majority of differentially expressed genes were down-regulated in advanced atherosclerotic plaques. For example, genes involved in immune and inflammatory responses (arachidonic acid metabolism, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, Jak-STAT signaling pathway, TNF signaling pathway) were down-regulated in advanced atherosclerotic plaques as compared with the healthy arteries. The most significant biological process of genes down-regulated in carotid atherosclerotic plaques (compared to intact internal thoracic arteries) was "cellular response to metal ions" (metallothioneins), and for upregulated genes was the organization of the extracellular matrix.



CLEC-2-Induced Signaling in Blood Platelets

Abstract

Platelet activating lectin-like receptor type C-2 (CLEC-2) has been identified on the platelet surface about a decade ago. The only known endogenous CLEC-2 agonist is podoplanin, a transmembrane protein expressed by lymphatic endothelial cells, lymph node fibroblastic reticular cells, kidney podocytes, and by cells of certain tumors. CLEC-2 and podoplanin are involved in metastases, sepsis, development of deep-vein thrombosis. The CLEC-2 signaling cascade includes tyrosine kinases (Syk, SFK, Btk) as well as LAT adapter, and phospholipase Cγ2, which induces calcium signalling. CLEC-2, podoplanin and proteins, participating in the CLEC-2 signalling cascade, are considered as perspective targets for antithrombotic therapy.



Aryl-Hydrocarbon Receptor as a Potential Target for Anticancer Therapy

Abstract—Aryl-hydrocarbon receptor (Aryl Hydrocarbon Receptor, AHR) is a ligand-dependent transcription factor; its functions are related to xenobiotic detoxification, response to inflammation, and the maintenance of tissue homeostasis. Results of recent studies suggest that AHR also plays an important role in carcinogenesis. Increased expression of AHR is observed in several types of tumors and tumor derived cell lines. In addition, many AHR ligands are included in compositions of pharmaceutical drugs used in oncotherapy. These facts provide some ground to consider AHR as a potential target for anticancer therapy, especially for treatment of severe cancers which have very limited (if any) treatment options. In this review we have considered the examples of the effects of AHR ligands on tumor derived cell cultures and on model mice lines with analysis of the AHR-dependent response.



Genetic and Biochemical Features of the Monogenic Hereditary Kidney Stone Disease

Abstract

Kidney stone disease (KSD) is a common urological problem. In most cases, this multifactorial pathology develops due to the combination of inherited low-penetrance gene variants and environment factors such as urinary tract infections, unbalanced diet, and gastrointestinal tract diseases with malabsorption. However, some cases of KSD represent monogenic diseases in which mutations are inherited from one or both parents. These hereditary forms of KSD, manifested in childhood, are characterized by multiple, bilateral or recurrent kidney stones and the earlier development of chronic kidney disease. Introduction of methods for exome and gene panels (NGS—Next Generation Sequencing) sequencing, significantly increased the proportion of detectable genetically identifiable hereditary forms of KSD in the group of patients under 18 year old, which exceeded 20%. In this review we have analyzed genetic and biochemical mechanisms of the main hereditary forms of KSD, associated with enzymopathies (primary hyperoxaluria, adenine phosphoribosyltransferase deficiency, phosphoribosylpyrophosphate synthetase superactivity, xanthinuria, Lesch-Nyhan syndrome) and impaired membrane transport (Dent's disease, familial hypomagnesemia with hypercalciuria and nephrocalcinosis, hypophosphatemic urolithiasis, distal tubular acidosis, cystinuria, Bartter's syndrome). We suggest a comprehensive gene panel for NGS diagnostics of the hereditary KSD. Accurate and timely diagnosis of hereditary forms of urolithiasis makes it possible to identify a pathological germinal mutation and make an accurate diagnosis, to analyze the heterozygous mutation carriage in affected families and evaluate the prognosis of KSD development in family members, as well as to perform personalized management of the patients with KSD.



Prospects for the Use of Gene Expression Analysis in Rheumatology

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory disease of unknown etiology, characterized by disturbances in functioning of immune system signaling pathways. This is accompanied by injury of other tissues, pain and joint destruction. Modern treatment is aimed at correction of pathophysiological and biochemical mechanisms responsible for clinical manifestation of the disease. However, due to heterogeneity of RA patients there is a clear need in the personalized approach to the RA treatment; the choice of personalized treatment is complicated by the variability of RA patient responses to the therapy. Gene expression analysis may serve as a tool for the disease control and therapy personification, for inhibition of inflammation and pain as well as for prevention of joint destruction.



Factors Governing Development of Non-Alcoholic Fatty Liver Disease and Insulin Resistance in Obesity

Abstract

The factors promoting development of non-alcoholic fatty liver disease in patients with obesity and different state of carbohydrate metabolism have been investigated in 43 patients. These included 26 patients with abdominal obesity (BMI = 52.9 ± 7.9 kg/m2) and 17 conditionally healthy donors (control group) without obesity (BMI = 18.9–24.9 kg/m2). Seven (of 17) conditionally healthy persons formed a comparison group that was included to compare the results of study on the levels of tissue-specific expression of HSP70 mRNA. The study of mRNA expression was performed by real-time PCR. Blood serum concentrations of IL-6 and TNF-α were evaluated by the ELISA method. In obese patients with diabetes mellitus type 2 (DM2), a significant increase in the serum level of pro-inflammatory cytokines was found in comparison with the group of patients without DM2 and control. The results of histological examination of liver biopsy specimens in obese patients revealed the most pronounced changes in the group of DM2 patients. Regardless of the stage of nonalcoholic fatty liver disease in obese DM2 patients, there was an increase in the area of fatty inclusions (relative to the group without DM2). The study of the HSP70 gene expression in peripheral blood mononuclear cells revealed a significant increase versus the comparison group. The relationship between the level of the HSP70 gene expression in metabolically active tissues (visceral, subcutaneous adipose tissue and liver) and the serum content of proinflammatory cytokines (IL-6 and TNF-α) found in all obese patients may indicate suppression of HSP70 expression in these tissues under conditions of systemic and local inflammation in obesity.



Neutrophils as a Source of Factors Increasing Duration of the Inflammatory Phase of Wound Healing in Patients with Type 2 Diabetes Mellitus

Abstract

Oxidative stress and neutrophil activation leading to an increase in myeloperoxidase (MPO), elastase and neutrophil extracellular trap (NET) levels in blood are considered as the pathogenic mechanisms responsible for the development of extremity damage in people with type 2 diabetes mellitus (T2DM). The aim of this study was to analyze the relationship between factors, associated with neutrophil activation, and the duration of the initial phase of wound healing (the inflammatory phase) in T2DM patients. Patients were divided retrospectively into three groups depending on the severity of lower extent damage: group 1 (wound on toe) < group 2 (wound on foot) < group 3 (wound on lower leg). At admission to hospital, T2DM patients had significantly higher (р < 0.05) levels of blood glucose and glycated hemoglobin (groups 1−3), ESR (groups 1 and 3), blood neutrophil count (groups 2 and 3), plasma MPO concentration (groups 1−3) and blood NET concentration (group 3) and decreased levels of plasma thiols (groups 1−3) and erythrocyte glutathione peroxidase activity (groups 2 and 3) than in the control group (healthy volunteers). The length of hospital stay after surgery corresponded to the length of the inflammatory phase of the wound healing process and correlated with the number of blood neutrophils in patients before surgery (r = 0.72, p < 0.05). Leukocytic intoxication index depended on wound area (r = 0.59, р < 0.05), and it was significantly higher for groups 2 and 3 compared to the control group and group 1. The correlation found can be attributed to the increase in extracellular MPO and NETs due to the activation and degranulation of neutrophils and netosis. Thus, the duration of the inflammatory phase of wound healing depends on specific aspects of systemic inflammation increasing oxidative/halogenative stress and intoxication.



The Study of the Neuroprotective Effects of Carnosine in the Experimental Model of Focal Cerebral Ischemia/Reperfusion

Abstract

Oxidative stress is one of the key factors responsible for brain tissue damage in ischemia; this points to the use of antioxidants under these conditions. One of the promising antioxidants for the therapy of ischemic stroke is a natural dipeptide carnosine. In this study the neuroprotective effect of dietary carnosine administration has been investigated in an experimental model of focal cerebral ischemia/reperfusion in Wistar rats. Animals received carnosine with a diet at a daily dose of 150 mg/kg for 7 days before transient occlusion of the middle cerebral artery (MCA), performed for 60 min. At 24 h after the onset of ischemia the effect of carnosine administration on the area of the infarct size was evaluated in animals. In brain tissue of animals the content of malondialdehyde (MDA), protein carbonyls (PC), total antioxidant capacity (TAC), total activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione transferase (GT), the content of isoprostanes and cytokines were measured. Treatment with carnosine significantly reduced the infarct size, increased TAC, and decreased the levels of MDA and isoprostanes in the brain tissue. Thus carnosine consumed prophylactically with the diet for 7 days before the ischemia induced by MCA occlusion in rats exhibited the direct neuroprotective effect, maintained high antioxidant activity of the brain tissue, reduced the level of oxidative damage markers (MDA and isoprostanes) but had no effect on the activity of antioxidant enzyme systems and production of cytokines in the brain tissue.



Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
6948891480

Medicine by Alexandros G. Sfakianakis


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Alexandros Sfakianakis
Anapafseos 5 . Agios Nikolaos
Crete.Greece.72100
2841026182
6948891480

Αρχειοθήκη ιστολογίου