Role of hypoxia-related proteins in invasion of ameloblastoma cells: crosstalk between NOTCH1, HIF-1α, ADAM-12, and HB-EGF

Abstract

Aims

Ameloblastoma (AME) is a benign tumor characterized by local invasiveness, high recurrence rates and diverse histological patterns. Oxygen concentration is reduced in specific areas of the tumor microenvironment, which leads to intratumoral hypoxia. Crosstalk between NOTCH1, ADAM-12, HIF-1α and HB-EGF under hypoxic conditions has been implicated in invadopodia formation, tumor invasiveness and metastasis development. The objective of this study was to analyze the expression of these proteins to further elucidate mechanisms underlying AME invasiveness.

Methods and results

A total of 20 cases of AME, 8 cases of calcifying cystic odontogenic tumors (CCOTs) and 10 samples of dental follicle were used to investigate the expression of these proteins by immunohistochemistry using the primary antibodies anti-NOTCH1, anti-ADAM-12, anti-HIF-1α and anti-HB-EGF. Immunostaining results were expressed as the percentage of stained area in images acquired in an AxioScope microscope equipped with an AxioCamHRc camera and a 40× objective. The results showed that immunoexpression of all proteins were higher in the AME samples than in the CCOT and dental follicle samples (p<0.05).

Conclusions

AME exhibited increased presence of proteins associated with tumor invasiveness, which indicates a possible role of these proteins in the biological behavior of this tumor.

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from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1OotrS4
via IFTTT