Immunohistochemical evaluation of the mTOR pathway in intraoral minor salivary gland neoplasms

Abstract

Objectives

The aim of this study was to investigate the expression of upstream and downstream molecules of the oncogenic mTOR signaling pathway in intraoral minor salivary gland tumors (SGTs).

Materials and Methods

Tissue samples consisted of 39 malignant and 13 benign minor SGTs, and 8 controls of normal minor salivary glands (NMSG). An immunohistochemical analysis for phosphorylated Akt, 4EBP1, S6 (total and phosphorylated), and eIF4E was performed.

Results

Expression of pAkt and 4EBP1 was found in all SGTs and in most NMSG. p4EBP1 was detected in almost all SGT cases, NMSG being negative. S6 immunoreactivity was observed in 37.5% of NMSG, 92.3% of benign and 100% of malignant SGTs, while pS6 expression was found in 77% of benign and 95% of malignant SGTs, but not in NMSG. Finally, eIF4E was expressed in 12.5% of NMSG, 69.2% of benign and 76.9% of malignant tumors. All molecules studied had statistically significantly lower expression in NMSG compared to SGTs. Moreover, malignant neoplasms received higher scores compared to benign tumors for all molecules with the exception of eIF4E.

Conclusion

The mTOR signaling pathway is activated in SGTs, especially in malignancies. Therefore, the possible therapeutic role of targeting the mTOR pathway by Rapamycin analogs in SGTs needs further investigation.

This article is protected by copyright. All rights reserved.

from #ΓναθοΧειρουργική via xlomafota13 on Inoreader http://ift.tt/1ZPDEhB
via IFTTT Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174

from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1Ww4MER
via IFTTT