Short communication: Labeling Listeria with anaerobic fluorescent protein for food safety studies

Publication date: Available online 9 November 2016
Source:Journal of Dairy Science
Author(s): José M. Landete, Ángela Peirotén, Margarita Medina, Juan L. Arqués
Many food safety-related studies require the tracking of inoculated food-borne pathogens to monitor their fate in food complex environments. In the current study, we demonstrate the potential of plasmids containing the fluorescence protein gene evoglow-Pp1 (Evocatal, Dusseldorf, Germany) as a real-time reporter system for Listeria strains. This anaerobic fluorescent protein provides an easily detectable phenotype of microorganisms for food safety studies. This work is the first to report a reliable method to identify fluorescently labeled Listeria strains in food ecosystems.



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