Diamond-based electrochemical aptasensor realizing a femtomolar detection limit of bisphenol A

Publication date: 15 June 2017
Source:Biosensors and Bioelectronics, Volume 92
Author(s): Yibo Ma, Junsong Liu, Hongdong Li
In this study, we designed and fabricated an electrochemical impedance aptasensor based on Au nanoparticles (Au-NPs) coated boron-doped diamond (BDD) modified with aptamers, and 6-mercapto-1-hexanol (MCH) for the detection of bisphenol A (BPA). The constructed BPA aptasensor exhibits good linearity from 1.0×10^–14 to 1.0×10⁻⁹molL⁻¹. The detection limitation of 7.2×10^–15molL⁻¹ was achieved, which can be attributed to the synergistic effect of combining BDD with Au-NPs, aptamers, and MCH. The examine results of BPA traces in Tris-HCl buffer and in milk, UV spectra of aptamer/BPA and interference test revealed that the novel aptasensors are of high sensitivity, specificity, stability and repeatability, which could be promising in practical applications.



http://ift.tt/2kfRw6O