Hypoxia reduces the E-cadherin expression and increases OSCC cell migration regardless of the E-cadherin methylation profile

Publication date: Available online 20 February 2017
Source:Pathology - Research and Practice
Author(s): Patrícia Luciana Batista Domingos, Marcela Gonçalves Souza, Talita Antunes Guimarães, Eliane Sobrinho Santos, Lucyana Conceição Farias, Carlos Alberto de Carvalho Fraga, Kimberly Marie Jones, Sérgio Henrique Souza Santos, Alfredo Maurício Batista de Paula, André Luiz Sena Guimarães
ObjectiveThe purpose of the current study is to investigate the association between E-cadherin methylation status, hypoxia and OSCC.MethodsHaCat and SCC9 cell lines were submitted to hypoxic treatment, followed by methylation profile analysis (MS-PCR) and analysis of the expression of mRNA gene E-cadherin (RT-PCR). Study group samples comprise individuals affected by potentially malignant lesions Potential Malignant Oral Lesion (PMOL, n=18) and oral squamous cell carcinoma (OSCC, n=28). The control group oral mucosa (OM, n=15) of patients with an oral mucocele. Cell migration ability was evaluated a scratch wound assay in SCC9 and HaCat cell linesResultsE-cadherin mRNA expression in the cell lines SCC9 and HaCat was significantly reduced under hypoxia, regardless of the methylation profile, when compared to the control group. No differences in methylation profile of the E-cadherin were observed among the groups OM, PMOL and OSCC. HaCat and SCC9 presented increases in cell migration rates under hypoxia.ConclusionThe current study demonstrates that hypoxia reduces E-cadherin expression and increase cell migration, regardless of the methylation profile. Additionally, no differences in E-cadherin methylation patterns were observed among OM, PMOL and OSCC.



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