Metabolomic changes in rat model of cauda equina injury.

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Metabolomic changes in rat model of cauda equina injury.

World Neurosurg. 2017 Mar 24;:

Authors: Liu Y, Yang R, Kong Q, Wang Y, Zhang B, Sun J, Yang Y, Zheng B, Yuan H, Shi J

Abstract
BACKGROUND: To demonstrate the differences of metabolomic changes in a rat model of cauda equina injury (CEI) and find potent metabolic biomarkers of CEI.
MATERIAL AND METHODS: A total of 28 Sprague-Dawley (SD) rats were used in this study. After the rats were given anesthesia and fixed in a prone position, a piece of silicone block was placed into the epidural space below the lamina. Behavior tests including the Basso, Beattie, and Bresnahan (BBB) open field locomotor scale and an inclined plane test were conducted 1 day and 2 days after surgery. The cauda equina tissue was collected 12 h, 1 day, and 2 days after surgery. Ultra-performance liquid chromatography, coupled with quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was used for a quantitative analysis of cauda equine metabolic changes in rats from different groups. The differences between the metabolic profiles of the rats in 4 groups were analyzed using partial least squares discriminant analysis (PLS-DA).
RESULTS: In behavior tests and histological analyses given 2 days after surgery, the animals exhibited remarkable organ dysfunction and pathological damage. At the same time, metabolic profiles showed remarkable differences between the control and model groups. 34 potential CEI metabolite biomarkers were identified between the control group and different time-point model groups. These potential biomarkers appeared in 15 metabolic pathways.
CONCLUSION: Our results may improve the etiology of CEI and provide a basis for clinical diagnosis and locating biomarkers in the early stages of the pathological process of CEI.

PMID: 28347895 [PubMed - as supplied by publisher]

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