Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells.

Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells.

Surg Infect (Larchmt). 2017 Mar 07;:

Authors: Pérez-Tanoira R, Aarnisalo AA, Eklund KK, Han X, Soininen A, Tiainen VM, Esteban J, Kinnari TJ

Abstract
BACKGROUND: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation.
METHODS: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1 × 10(5) cells/mL), the materials were incubated for 4.5 hours or two days with Staphylococcus aureus in serial 1:10 dilutions of 10(8) colony-forming units/mL. The bacterial adherence and biofilm biomass on materials pre-incubated with SaOS-2 cells were compared with our previous results on materials incubated only with bacteria or in simultaneous co-culture of SaOS-2 cells and S. aureus. Fluorescent microscopy and crystal violet stain were used. The number of viable SaOS-2 and bacterial cells present was tested using colorimetric methods (MTT, LDH) and drop plate method, respectively.
RESULTS: The pre-treatment with human cells was associated with a reduction of bacterial colonization of the biomaterial at 4.5 hours and 48 hours compared with the non-pre-treated materials. The presence of bacteria decreased the number of viable human cells on all materials. ( Supplementary Fig. 1 ; see online supplementary materials at http://ift.tt/2ml6Su7 ).
CONCLUSIONS: These results suggest that the pre-operative incubation of prostheses with host cells could prevent infection of biomaterials.

PMID: 28267427 [PubMed - as supplied by publisher]

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