Publication date: Available online 9 March 2017
Source:Journal of Dairy Science
Author(s): Yueming Zhao, Xia Jiang, Yanyan Qu, Ruili Pan, Xinyi Pang, Yujun Jiang, Chaoxin Man
In this study, we developed a rapid, specific, and sensitive loop-mediated isothermal amplification technique combined with a lateral flow dipstick (LAMP-LFD) method to detect Salmonella targeting the siiA gene in powdered infant formula (PIF). The specificity of the detection method (LAMP-LFD) approached 100% using 21 Salmonella and 31 non-Salmonella bacterial strains. This detection method exhibited high sensitivity limits for pure cultures at 3.7 cfu/mL and in PIF at 2.2 cfu/g without enrichment. To evaluate the applicability of the LAMP-LFD method, we detected 60 positive PIF samples and 20 negative PIF samples. The results showed that the method of LAMP-LFD had a high diagnostic specificity of 100% for detection of Salmonella in PIF. To reduce incidence of LAMP contamination, we applied propidium monoazide (PMA) to eliminate carryover contamination of LAMP. At the same time, we found that PMA does not affect observation of LFD for measurement of LAMP signal. The results verified that the method of LAMP-LFD targeting the siiA gene is rapid, accurate, and sensitive for Salmonella detection in PIF, and that PMA shows great potential to be widely used to eliminate the amplicon contamination risk generated by the highly sensitive LAMP reaction in the detection process.
http://ift.tt/2lHhe9i
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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- Voice and swallowing outcomes for adults undergoin...
- The use of practice guidelines in the management o...
- Self-reported postoperative recovery in children a...
- Foreign body aspiration in children: Focus on the ...
- Anti-angiogenic treatment for breast cancer?
- The role of drug-drug interactions in prostate can...
- Incidental Findings on Pediatric Abdominal Magneti...
- Dosimetric Analysis of Liver Toxicity Post Liver M...
- Outcomes and Toxicity following High-Dose Radiatio...
- Persistent nasal carriers of Acinetobacter baumann...
- A New Keystone Flap “Plus” Design: Case Series and...
- Failure to Diagnose an Incompetent Cervix,Prematur...
- Permanent prostate brachytherapy pubic arch evalua...
- Assessment of clinical and radiologic differences ...
- Use of hepatobiliary phase images in Gd-EOB-DTPA-e...
- Unusual ultrasound appearance of small bowel intus...
- Contrast-enhanced ultrasound and computed tomograp...
- Neocortical and hippocampal TREM2 protein levels d...
- Engineering intravaginal vaccines to overcome muco...
- Islet encapsulation with polyphenol coatings decre...
- Tolerability, usability and acceptability of disso...
- TANTIGEN: a comprehensive database of tumor T cell...
- Murine Th17 cells utilize IL-2 receptor gamma chai...
- Acoustic Context Alters Vowel Categorization in Pe...
- Verrucarin A and roridin E produced on spinach by ...
- Withdrawing ASV therapy in clinical practice: tria...
- Risk of developing obstructive sleep apnea among w...
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- Reactivating p53 and Inducing Tumor Apoptosis (RIT...
- Noninvasive Bioluminescence Imaging of AKT Kinase ...
- Emergency cricothyrotomy: temporary measure or def...
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- The clinical manifestations of vestibular migraine...
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- Withered on the stem: is bamboo a seasonally limit...
- Frequencies of erythrocyte nuclear abnormalities a...
- Using early life stages of marine animals to scree...
- Toxic effect of nonylphenol on the marine macroalg...
- Unique characteristics of algal dissolved organic ...
- Precipitation and air temperature control the vari...
- The death of the circulatory system diseases in Ch...
- Slow-release formulations of the herbicide piclora...
- Migration of nitrate, nitrite, and ammonia through...
- Investigations of temperature and pH variations on...
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- Enhanced heterogeneous photo-Fenton process modifi...
- Characterization of ferromagnetic sludge-based act...
- High contribution of the particulate uptake pathwa...
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- Our auditory results using the Vibrant Soundbridge...
- Addiction: an underestimated problem in psoriasis ...
- The clinical manifestations of vestibular migraine...
- Our auditory results using the Vibrant Soundbridge...
- The clinical manifestations of vestibular migraine...
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- Hypofractionated radiotherapy in older patients wi...
- Vitamin D supplementation in pregnancy, prenatal 2...
- Three-dimensional cardiac microtissues composed of...
- Engineering intravaginal vaccines to overcome muco...
- G protein-coupled calcium-sensing receptor is a cr...
- Tolerability, usability and acceptability of disso...
- Surface engineering of semiconducting polymer nano...
- Incidence of hidradenitis suppurativa in the Unite...
- Calciphylaxis: Comparison of radiologic imaging an...
- Pediatric Stevens-Johnson syndrome and toxic epide...
- Histopathological changes in morphea and their cli...
- Proximal upper limb jerking: important clinical si...
- Graphene oxide functionalized long period grating ...
- Quantum-dots-based photoelectrochemical bioanalysi...
- Label-free identification of activated T lymphocyt...
- Nanosphere-based one-step strategy for efficient a...
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