Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED

Publication date: Available online 13 April 2017
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Cintia R. Lima Leal, Letícia H. Alvarenga, Tamires Oliveira-Silva, Ilka T. Kato, Bianca Godoy-Miranda, Sandra K. Bussadori, Martha S. Ribeiro, Renato A. Prates
BackgroundDental caries are multifactorial disease and progressively produces tooth destruction as result from bacterial colonization of enamel surface, especially Streptococcus mutans. The objective of this work was to investigate the role of glucose in antimicrobial photodynamic therapy (aPDT) on S. mutans.MethodsS. mutans ATCC 25175 were cultured on microaerophilia at 37°C for 48h, and we tested aPDT in the presence of 50mM glucose. Bacterial suspension was used to investigate aPDT with 100μM methylene blue (MB) under LED emitting radiation at ʎ=660nm and parameters as following (P=473 mW; I=166.8 mW/cm², and doses of 5, 10 and 20J/cm²). A seventy-two hours biofilm was grown on 96 flat buttoned well-plate and irradiation was performed from 10 to 80J/cm² at similar conditions.ResultsThere was no dark toxicity nor bacterial death regarding LED irradiation on suspension and on biofilm. Nevertheless, aPDT presented expressive bacterial inactivation following 1 and 2min of irradiation on cell suspension. On the other hand, there was no inactivation in the presence of glucose under the same conditions. Biofilm was completely inactivated by MB-mediated aPDT after 6min of irradiation. However, the presence of glucose delayed the complete inactivation of the biofilm.ConclusionThe presence of glucose in the suspension drastically delayed the effect of aPDT on S. mutans and this effect is pronounced in bacterial suspension than on biofilm.



http://ift.tt/2pge8Kc