High throughput differential identification of TMPRSS2-ERG fusion genes in prostate cancer patient urine

Publication date: August 2017
Source:Biomaterials, Volume 135
Author(s): Hyojin Lee, Dongjin Lee, Jea Ho Park, Sang Hoon Song, In Gab Jeong, Choung-Soo Kim, Peter C. Searson, Kwan Hyi Lee
Identifying genetic diversity is important for studies in cancer as it can provide insights on disease progression and treatment. Although clinical outcome and major symptom of cancer might be same in all patients, the type of overexpressed gene could be different. Even though prostate-specific antigen assay is a good tool widely used for prostate cancer diagnosis, it is not capable of providing information on genetic differences. Therefore, screening method that can differentiate genetic differences is necessary. Here we detected different types of TMPRSS2-ERG, prostate cancer specific fusion genes, to verify the genetic diversity between the patients using high throughput screening method, bio-barcode assay. Prostate cancer patients with different types of fusion gene were successfully differentiated directly from untreated patients' urine, while traditional PSA assay could not. This non-invasive assay, when used with PSA assay, can be a strong secondary screening method which can offer new insights on disease progression and clinical outcome.



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