Preclinical transgenic and patient-derived xenograft models recapitulate the radiological features of human adamantinomatous craniopharyngioma

Abstract

Aim: To assess the clinical relevance of transgenic and patient-derived xenograft models of adamantinomatous craniopharyngioma (ACP) using serial magnetic resonance imaging (MRI) and high resolution post-mortem micro-computed tomography (μ-CT), with correlation with histology and human ACP imaging.

Methods: The growth patterns and radiological features of tumours arising in Hesx1^Cre/+;Ctnnb1^l°^x(ex3)/+ transgenic mice, and of patient-derived ACP xenografts implanted in the cerebral cortex, were monitored longitudinally in vivo with anatomical and functional MRI, and by ex vivo μ-CT at study end. Pathological correlates with haematoxylin and eosin stained sections were investigated.

Results: Early enlargement and heterogeneity of Hesx1^Cre/+;Ctnnb1^l°^x(ex3)/+ mouse pituitaries was evident at initial imaging at 8 weeks, which was followed by enlargement of a solid tumour, and development of cysts and haemorrhage. Tumours demonstrated MRI features that recapitulated those of human ACP, specifically, T₁-weighted signal enhancement in the solid tumour component following Gd-DTPA administration, and in some animals, hyperintense cysts on FLAIR and T₁-weighted images. Ex vivo μ-CT correlated with MRI findings and identified smaller cysts, which were confirmed by histology. Characteristic histological features, including wet keratin and calcification, were visible on μ-CT and verified by histological sections of patient-derived ACP xenografts.

Conclusions: The Hesx1^Cre/+;Ctnnb1^l°^x(ex3)/+ transgenic mouse model and cerebral patient-derived ACP xenografts recapitulate a number of the key radiological features of the human disease and provide promising foundations for in vivo trials of novel therapeutics for the treatment of these tumours. This article is protected by copyright. All rights reserved.

http://ift.tt/2pVVkQd