Increased Triacylglycerol Lipase Activity in Adipose Tissue of Lean and Obese Men During Endurance Exercise.

Increased Triacylglycerol Lipase Activity in Adipose Tissue of Lean and Obese Men During Endurance Exercise.

J Clin Endocrinol Metab. 2017 Jun 12;:

Authors: Petridou A, Chatzinikolaou A, Avloniti A, Jamurtas A, Loules G, Papassotiriou I, Fatouros I, Mougios V

Abstract
Context: Although there is increasing information on the mechanism of lipolysis in adipose tissue, the effect of exercise on individual factors of lipolysis is less well understood.
Objective: We aimed to compare changes in adipose tissue triacylglycerol lipase activity and gene expression of adipose triacylglycerol lipase (ATGL), hormone sensitive lipase (HSL), monoacylglycerol lipase (MGL), perilipin 1, and comparative gene identification 58 (CGI-58) during exercise between lean and obese men.
Design and Participants: 7 lean and 9 obese men cycled for 30 min at a heart rate of 130-140 beats min-1. At baseline and 5, 10, 20, and 30 min of exercise we sampled subcutaneous adipose tissue for triacylglycerol lipase activity and mRNA determination, and blood for glycerol, non-esterified fatty acid (NEFA), glucose, lactate, insulin, and catecholamine determination.
Setting: The study was conducted at a university research unit.
Results: Triacylglycerol lipase activity increased at 10 min of exercise in the lean and returned to baseline at 20 and 30 min. In the obese, it was higher than baseline at 10, 20, and 30 min, and higher than the corresponding values in the lean at 20 and 30 min. No changes in mRNA levels were found during exercise, but the obese had lower mRNA levels of ATGL, HSL, and CGI-58, compared to the lean.
Conclusions: Our findings suggest different patterns of lipolytic stimulation during endurance exercise between lean and obese men. Differences in lipolytic rate seem to be due to differences in protein amount or activity, not mRNA levels.

PMID: 28605462 [PubMed - as supplied by publisher]

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