Publication date: September 2017
Source:Biomaterials, Volume 138
Author(s): Kkotchorong Park, Keun Cheon Kim, Hyoban Lee, Yoori Sung, Mijeong Kang, Yun Mi Lee, Ji Yeon Ahn, Jeong Mook Lim, Taejoon Kang, Bongsoo Kim, Eun Ju Lee
Transgenic animals have become key tools in a variety of biomedical research areas. However, microinjection commonly used for producing transgenic animals has several challenges such as physical and chemical damage to the embryos due to microinjector with buffer, and low transgene integration rates with frequent mosaicism. Here, we report direct delivery of plasmids into mouse embryos using a Au nanowire injector (NWI) that significantly improved transgene integration efficiency and suppressed mosaicism. The Au NWI could deliver plasmid into the pronucleus (PN) of a mouse zygote without buffer and rapidly release it with electric pulse. Because zygote, which is a fertilized 1-cell stage embryo, has two physical barriers (cytoplasmic membrane and zona pellucida), direct delivery of plasmids into PN of zygote is more difficult than into a normal cell type. To penetrate the two physical barriers with minimal disruption of the embryo, we optimized the diameter and length of Au NWI. The mosaicism is more reduced in the Au NWI injected embryos than in micropipette injected embryos, which was determined by the expression of transgene in a blastocyst stage. We suggest that Au NWI can increase the efficiency of gene delivery into zygote with suppressed mosaicism and become a useful alternative.
http://ift.tt/2rBGsp2
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Ιουνίου
(10489)
-
▼
Ιουν 05
(369)
- New Method for Sex Prediction Using the Human Non-...
- Investigation of a correlation between taxane-base...
- Proteomic profiling of the brain of mice with expe...
- Carbon monoxide protects against hepatic steatosis...
- Knowledge, aptitudes, and preferences in implant d...
- Letter to Editor: Support to the Spanish language,...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- Uncommon oral manifestation of lichen sclerosus: c...
- What is the frequency of anatomical variations and...
- Effect of pre-operatory low-level laser therapy on...
- Accuracy of cotinine serum test to detect the smok...
- Slow-5 dynamic functional connectivity reflects th...
- Optimal use of EEG recordings to target active bra...
- Immunosurveillance profile of oral squamous cell c...
- Functional and phenotypic analysis of basophils al...
- Effects of Substrate Stiffness on the Tenoinductio...
- Uncommon oral manifestation of lichen sclerosus: c...
- Effect of pre-operatory low-level laser therapy on...
- Letter to Editor: Support to the Spanish language,...
- Knowledge, aptitudes, and preferences in implant d...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- What is the frequency of anatomical variations and...
- Accuracy of cotinine serum test to detect the smok...
- Endoscopic endonasal management of esthesioneurobl...
- Functional and phenotypic analysis of basophils al...
- Endoscopic endonasal management of esthesioneurobl...
- Comparison of two skin imaging analysis instrument...
- A Diagnostic Accuracy Meta-analysis of CT and MRI ...
- Uncommon oral manifestation of lichen sclerosus: c...
- Letter to Editor: Support to the Spanish language,...
- Knowledge, aptitudes, and preferences in implant d...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- What is the frequency of anatomical variations and...
- Effect of pre-operatory low-level laser therapy on...
- Accuracy of cotinine serum test to detect the smok...
- Combined interventional sialendoscopy and intraduc...
- Combined interventional sialendoscopy and intraduc...
- Association of atmospheric concentrations of polyc...
- Variability of zinc, copper and lead contents in s...
- Ecotoxicological assessment of pesticides and thei...
- Distribution, sources, and ecological risk assessm...
- Long-term dynamic characterization of aeolian dese...
- Inside Front Cover - Editorial Board Page/Cover im...
- Caution Regarding Government-Mandated Shared Decis...
- Here Comes Trouble: A Career as a Tobacco Control ...
- Randomized Comparison of Paclitaxel-Eluting Balloo...
- Composite End Points in Clinical Research: A Time ...
- Low-Dose Paclitaxel-Coated Versus Uncoated Percuta...
- Novel Strategies to Reduce Femoropopliteal Resteno...
- Fractional Flow Reserve and Cardiac Events in Coro...
- Real-Life Fractional Flow Reserve.
- Genotype-Phenotype Correlation of SCN5A Mutation f...
- Left Ventricular Dysfunction Switches Mesenchymal ...
- Modulation of Endothelial Bone Morphogenetic Prote...
- From the Literature.
- Safety of Sports for Athletes With Implantable Car...
- Association of Feature-Tracking Cardiac Magnetic R...
- Letter by Moris et al Regarding Article, "Ticagrel...
- Letter by Siasos et al Regarding Article, "Ticagre...
- Response by Jones et al to Letter Regarding Articl...
- Letter by Harbaoui et al Regarding Article, "Coron...
- Response by McEvoy and Blaha to Letter Regarding A...
- Genetic and geochemical signatures to prevent frau...
- Catalytic pyrolysis and gasification of waste text...
- Modeling the C9ORF72 repeat expansion mutation usi...
- A 53-Year-Old Woman with a Subfascial Mass of the ...
- Modeling tau pathology in human stem cell derived ...
- Modeling Parkinson's disease with induced pluripot...
- A 32-Year-Old Man with Headache and Visual Loss
- Understanding neurodevelopmental disorders using h...
- Issue Information
- 19-Year-Old Male with Headaches and a Possible Sei...
- Astrocytes in a dish: Using pluripotent stem cells...
- AN 11-Year-Old Boy with a Leptomeningeal Tumor
- SOCIETY NEWS
- Endoscopic endonasal management of esthesioneurobl...
- Contribution of Simultaneous Breast Reconstruction...
- Long-term outcomes of central neck dissection for ...
- The effects of fibroblast growth factor-2 delivere...
- Utility of Laryngeal High-speed Videoendoscopy in ...
- Menstrual Cycle, Vocal Performance, and Laryngeal ...
- “Cold-Steel” Phonosurgery of Reinke Edema Evaluate...
- Acoustic Analysis of Voice and Electroglottography...
- Aerodynamic Patterns in Patients With Voice Disord...
- Long-term outcomes of central neck dissection for ...
- The effects of fibroblast growth factor-2 delivere...
- Scar Management After Surgical Repair of Lateral F...
- Clinical Analysis of MatrixMANDIBLE Preformed Reco...
- F-18 FDG and F-18 Tau PET in posterior cortical at...
- Curcumin Protects Against Acoustic Trauma in the R...
- Clinical role of electrocochleography in children ...
-
▼
Ιουν 05
(369)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Ιουνίου
(10489)
-
▼
Ιουν 05
(369)
- New Method for Sex Prediction Using the Human Non-...
- Investigation of a correlation between taxane-base...
- Proteomic profiling of the brain of mice with expe...
- Carbon monoxide protects against hepatic steatosis...
- Knowledge, aptitudes, and preferences in implant d...
- Letter to Editor: Support to the Spanish language,...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- Uncommon oral manifestation of lichen sclerosus: c...
- What is the frequency of anatomical variations and...
- Effect of pre-operatory low-level laser therapy on...
- Accuracy of cotinine serum test to detect the smok...
- Slow-5 dynamic functional connectivity reflects th...
- Optimal use of EEG recordings to target active bra...
- Immunosurveillance profile of oral squamous cell c...
- Functional and phenotypic analysis of basophils al...
- Effects of Substrate Stiffness on the Tenoinductio...
- Uncommon oral manifestation of lichen sclerosus: c...
- Effect of pre-operatory low-level laser therapy on...
- Letter to Editor: Support to the Spanish language,...
- Knowledge, aptitudes, and preferences in implant d...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- What is the frequency of anatomical variations and...
- Accuracy of cotinine serum test to detect the smok...
- Endoscopic endonasal management of esthesioneurobl...
- Functional and phenotypic analysis of basophils al...
- Endoscopic endonasal management of esthesioneurobl...
- Comparison of two skin imaging analysis instrument...
- A Diagnostic Accuracy Meta-analysis of CT and MRI ...
- Uncommon oral manifestation of lichen sclerosus: c...
- Letter to Editor: Support to the Spanish language,...
- Knowledge, aptitudes, and preferences in implant d...
- In vitro comparison of primary stability of two im...
- Assessment of time intervals in the pathway to ora...
- Mandibular advancement devices vs nasal-continuous...
- Oral health and systemic inflammatory, cardiac and...
- What is the frequency of anatomical variations and...
- Effect of pre-operatory low-level laser therapy on...
- Accuracy of cotinine serum test to detect the smok...
- Combined interventional sialendoscopy and intraduc...
- Combined interventional sialendoscopy and intraduc...
- Association of atmospheric concentrations of polyc...
- Variability of zinc, copper and lead contents in s...
- Ecotoxicological assessment of pesticides and thei...
- Distribution, sources, and ecological risk assessm...
- Long-term dynamic characterization of aeolian dese...
- Inside Front Cover - Editorial Board Page/Cover im...
- Caution Regarding Government-Mandated Shared Decis...
- Here Comes Trouble: A Career as a Tobacco Control ...
- Randomized Comparison of Paclitaxel-Eluting Balloo...
- Composite End Points in Clinical Research: A Time ...
- Low-Dose Paclitaxel-Coated Versus Uncoated Percuta...
- Novel Strategies to Reduce Femoropopliteal Resteno...
- Fractional Flow Reserve and Cardiac Events in Coro...
- Real-Life Fractional Flow Reserve.
- Genotype-Phenotype Correlation of SCN5A Mutation f...
- Left Ventricular Dysfunction Switches Mesenchymal ...
- Modulation of Endothelial Bone Morphogenetic Prote...
- From the Literature.
- Safety of Sports for Athletes With Implantable Car...
- Association of Feature-Tracking Cardiac Magnetic R...
- Letter by Moris et al Regarding Article, "Ticagrel...
- Letter by Siasos et al Regarding Article, "Ticagre...
- Response by Jones et al to Letter Regarding Articl...
- Letter by Harbaoui et al Regarding Article, "Coron...
- Response by McEvoy and Blaha to Letter Regarding A...
- Genetic and geochemical signatures to prevent frau...
- Catalytic pyrolysis and gasification of waste text...
- Modeling the C9ORF72 repeat expansion mutation usi...
- A 53-Year-Old Woman with a Subfascial Mass of the ...
- Modeling tau pathology in human stem cell derived ...
- Modeling Parkinson's disease with induced pluripot...
- A 32-Year-Old Man with Headache and Visual Loss
- Understanding neurodevelopmental disorders using h...
- Issue Information
- 19-Year-Old Male with Headaches and a Possible Sei...
- Astrocytes in a dish: Using pluripotent stem cells...
- AN 11-Year-Old Boy with a Leptomeningeal Tumor
- SOCIETY NEWS
- Endoscopic endonasal management of esthesioneurobl...
- Contribution of Simultaneous Breast Reconstruction...
- Long-term outcomes of central neck dissection for ...
- The effects of fibroblast growth factor-2 delivere...
- Utility of Laryngeal High-speed Videoendoscopy in ...
- Menstrual Cycle, Vocal Performance, and Laryngeal ...
- “Cold-Steel” Phonosurgery of Reinke Edema Evaluate...
- Acoustic Analysis of Voice and Electroglottography...
- Aerodynamic Patterns in Patients With Voice Disord...
- Long-term outcomes of central neck dissection for ...
- The effects of fibroblast growth factor-2 delivere...
- Scar Management After Surgical Repair of Lateral F...
- Clinical Analysis of MatrixMANDIBLE Preformed Reco...
- F-18 FDG and F-18 Tau PET in posterior cortical at...
- Curcumin Protects Against Acoustic Trauma in the R...
- Clinical role of electrocochleography in children ...
-
▼
Ιουν 05
(369)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου