Proinflammatory cytokine production from NOK-SI keratinocytes after exposure to denture adhesives

Publication date: Available online 8 July 2017
Source:The Journal of Prosthetic Dentistry
Author(s): Andressa Rosa Perin Leite, Norberto Martins de Oliveira Júnior, Danny Omar Mendoza Marin, Marco Antonio Compagnoni, Ana Carolina Pero
Statement of problemAlthough the cytotoxicity of denture adhesives has been widely assessed, data on the release of proinflammatory cytokines from oral epithelial cells are still scarce.PurposeThe purpose of this in vitro study was to evaluate the proinflammatory cytokines interleukin (IL) 1β, IL-6, and tumor necrosis factor α (TNF-α) released from human oral keratinocytes after exposure to denture adhesives.Material and methodsEluates of the Ultra Corega cream, Corega powder Fixador Ultra, and Corega strip denture adhesives were created at 1% wt/vol after 24 hours of exposure in a Dulbecco modified Eagle medium culture. The release of IL-1β, IL-6, and TNF-α was measured using a sandwich enzyme-linked immunosorbent assay after the oral epithelial cells (NOK-SI) had been exposed to the eluates for 3, 6, and 24 hours (n=6).ResultsIL-1β release from the strip was higher at 3 hours compared with the control (Bonferroni post hoc test, P=.016). IL-6 releases from the cream and powder denture adhesives at 6 and 24 hours were higher than for the controls (Bonferroni post hoc test, P<.05). TNF-α release was not detectable in some groups and periods.ConclusionsIn long periods, denture adhesive strips do not induce any change on cytokine release from human oral keratinocytes.



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