Publication date: September 2017
Source:Journal of Dairy Science, Volume 100, Issue 9
Author(s): K. Dieho, J. van Baal, L. Kruijt, A. Bannink, J.T. Schonewille, D. Carreño, W.H. Hendriks, J. Dijkstra
We previously reported 2 experiments with rumen-cannulated Holstein-Friesian dairy cows showing that during the transition period, rumen papillae surface area, and fractional absorption rate of volatile fatty acids (VFA) increase after calving. However, supplemental concentrate during the dry period and rate of increase of concentrate allowance during lactation affected papillae surface area, but not VFA absorption. Here we report the changes in gene and protein expression in rumen papillae related to tissue growth and VFA utilization. The lactation experiment treatment consisted of a rapid [RAP; 1.0 kg of dry matter (DM)/d; n = 6] or gradual (GRAD; 0.25 kg of DM/d; n = 6) increase of concentrate allowance (up to 10.9 kg of DM/d), starting at 4 d postpartum (pp). The dry period experiment treatment consisted of 3.0 kg of DM/d of concentrate (n = 4) or no concentrate (n = 5) during the last 28 d of the dry period. Real-time quantitative PCR analysis of rumen papillae showed that the expression of apoptosis-related genes was neither affected by day nor its interaction with treatment for both experiments. Expression of epithelial transporter genes was not affected by day or treatment in the lactation experiment, except for NBC1. In the dry period experiment, expression of MCT1, NBC1, DRA, NHE2, NHE3, and UT-B generally decreased after calving. A day and treatment interaction was observed for ATP1A1 in the dry period experiment, with greater expression at 18 and 8 d antepartum for concentrate than no concentrate. Generally, expression of VFA metabolism-related genes was not affected by day or its interaction with treatment. In the lactation experiment, immunoblotting of 5 selected genes showed that protein expression of DRA and PCCA was greater at 16 d pp compared with 3 and 44 d pp. Expression of NHE2 was greater, and that of ATP1A1 lower, at 16 and 44 d pp compared with 3 d pp, suggesting alterations in intracellular pH regulation and sodium homeostasis. Both MCT1 and PCCA protein were upregulated by RAP from 3 to 16 d pp, indicating modulations in VFA metabolism. Our data suggests that VFA absorption and metabolic capacity changed little per unit of surface area during the transition period, and suggests that a change in mitosis rate rather than apoptosis rate is associated with the increased ruminal VFA production, resulting in tissue growth. A significant but weak correlation between the examined gene and protein expression levels was observed only for PCCA, indicating that care must be taken when interpreting results obtained at either level.
http://ift.tt/2v0y17J
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Αυγούστου
(7262)
-
▼
Αυγ 19
(179)
- Skin suturing and cortical surface viral infusion ...
- Corrigendum to ‘A system to measure the pupil resp...
- A high-sensitive nano-modified biosensor for dynam...
- Covalent and non-covalent chemical engineering of ...
- Reputation Management and Content Control: An Anal...
- Autologous platelet-rich plasma versus conventiona...
- Autologous platelet-rich plasma versus conventiona...
- Effect of different dental burs for experimental i...
- Linkage between human population and trace element...
- Seasonality, diaspore traits and the structure of ...
- Neurobiology of autoimmune encephalitis
- New insights into olivo-cerebellar circuits for le...
- Amino acid functionalized zinc oxide nanostructure...
- Isolated Second Implant Adaptation Period in Seque...
- Musical Rehabilitation in Adult Cochlear Implant R...
- Linking Deafness and Dementia: Challenges and Oppo...
- Breast cancer subtype discrimination using standar...
- Metastable Zr–Nb alloys for spinal fixation rods w...
- The role of inattention and hyperactivity/impulsiv...
- CCR10 activation stimulates the invasion and migra...
- Progesterone therapy induces an M1 to M2 switch in...
- Collagenase treatment enhances proteomic coverage ...
- Degradable bioadhesive nanoparticles for prolonged...
- Failure modes and effects analysis for ocular brac...
- Conchal contractility after inferior turbinate hyp...
- Conchal contractility after inferior turbinate hyp...
- Trends in antibiotic prescribing by dental practit...
- Long-term therapy with intravenous zoledronate inc...
- Determining the fate of cranial sutures after surg...
- Perinatal complications in patients with unisutura...
- Effects of low-level laser therapy and platelet co...
- Development and Validation of a Modular Endoscopic...
- Diagnosis of Superior Semicircular Canal Dehiscenc...
- Significance of Endolymphatic Hydrops in Ears With...
- Vestibular Function in Adults With Epilepsy of Unk...
- Noonan Syndrome: An Underestimated Cause of Severe...
- Outcomes and Time to Emergence of Auditory Skills ...
- Audiologic Gain of Incus Short Process Vibroplasty...
- The Influence of Intraoperative Testing on Surgica...
- Reduction in Temporary and Permanent Audiological ...
- Role of Multislice CT Imaging in Predicting the Vi...
- Visual Processing Recruits the Auditory Cortices i...
- Can Preoperative CT Scans Be Used to Predict Facia...
- The Role of Preoperative Steroids in Atraumatic Co...
- Paediatric endonasal dacryocystorhinostomy using a...
- Effectiveness of Dietetic Consultations in Primary...
- Which patient with thyroid cancer deserves systemi...
- Expansion of blood IgG4+ Bcells, Th2 and Tregulato...
- Cardiac complexity and emotional dysregulation in ...
- Diagnostic implications of TERT promoter mutation ...
- Sarcomatoid carcinoma associated with small cell c...
- Spatial variation and toxicity assessment for heav...
- Guía de Práctica Clínica Para el Diagnóstico y Tra...
- Recombinant human IgG1 based Fc multimers, with li...
- IFC(EDITORIAL BOARD)
- DNA polymerase ι: the long and the short of it!
- A novel somatic JAK2 kinase-domain mutation in ped...
- Mentalizing regions represent distributed, continu...
- Decoding cognitive concepts from neuroimaging data...
- Suppression of irrelevant sounds during auditory w...
- Unidirectional brain to muscle connectivity reveal...
- MB-SWIFT functional MRI during deep brain stimulat...
- Simultaneous intrinsic signal imaging of auditory ...
- Differential expression of transforming growth fac...
- Evaluation of coronary artery disease as a risk fa...
- Coexistence of congenital left ventricular aneurys...
- Editorial Board
- Table of Contents
- Information for Authors
- Masthead
- Effects of transcranial direct current stimulation...
- Inter-hemispheric control of vestibular thresholds
- Brief rTMS delivered by H-Coil to a healthy volunt...
- Prevalence of high-risk human papillomavirus infec...
- Distribution and controls of heavy metals and orga...
- Mechanisms of Mixed Chimerism-Based Transplant Tol...
- Post-Translational Peptide Splicing and T Cell Res...
- Reproducibility and validity of the Italian versio...
- Coexistence of congenital left ventricular aneurys...
- Carapax Trionycis extracts inhibit fibrogenesis of...
- Berberine activates caspase-9/cytochrome c-mediate...
- Circadian variation in anticonvulsant activity of ...
- Up-regulation of HCRP1 inhibits proliferation and ...
- Phytanic acid induced neurological alterations in ...
- Nrf2 activation is required for curcumin to induce...
- Anti-photoaging potential of propolis extract in U...
- The prognostic landscape of tumor-infiltrating imm...
- Glycyrrhizic acid attenuates myocardial injury: In...
- Catalpol suppressed proliferation, growth and inva...
- VDAC1 deacetylation is involved in the protective ...
- Antidiabetic activity of hydro-alcoholic stem bark...
- Evaluation of dose calculations accuracy of a comm...
- A chemometric investigation of aromatic emission p...
- Nitrous acid in a street canyon environment: Sourc...
- The blue skies in Beijing during APEC 2014: A quan...
- Effect of relative humidity on O3 and NO2 oxidatio...
- Nano-sized and other improved reporters for Magnet...
- Experimental cocrystal screening and solution base...
- The Currarino Triad
- Iron deficiency anemia in children with cyanotic c...
-
▼
Αυγ 19
(179)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Σάββατο 19 Αυγούστου 2017
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
-
▼
Αυγούστου
(7262)
-
▼
Αυγ 19
(179)
- Skin suturing and cortical surface viral infusion ...
- Corrigendum to ‘A system to measure the pupil resp...
- A high-sensitive nano-modified biosensor for dynam...
- Covalent and non-covalent chemical engineering of ...
- Reputation Management and Content Control: An Anal...
- Autologous platelet-rich plasma versus conventiona...
- Autologous platelet-rich plasma versus conventiona...
- Effect of different dental burs for experimental i...
- Linkage between human population and trace element...
- Seasonality, diaspore traits and the structure of ...
- Neurobiology of autoimmune encephalitis
- New insights into olivo-cerebellar circuits for le...
- Amino acid functionalized zinc oxide nanostructure...
- Isolated Second Implant Adaptation Period in Seque...
- Musical Rehabilitation in Adult Cochlear Implant R...
- Linking Deafness and Dementia: Challenges and Oppo...
- Breast cancer subtype discrimination using standar...
- Metastable Zr–Nb alloys for spinal fixation rods w...
- The role of inattention and hyperactivity/impulsiv...
- CCR10 activation stimulates the invasion and migra...
- Progesterone therapy induces an M1 to M2 switch in...
- Collagenase treatment enhances proteomic coverage ...
- Degradable bioadhesive nanoparticles for prolonged...
- Failure modes and effects analysis for ocular brac...
- Conchal contractility after inferior turbinate hyp...
- Conchal contractility after inferior turbinate hyp...
- Trends in antibiotic prescribing by dental practit...
- Long-term therapy with intravenous zoledronate inc...
- Determining the fate of cranial sutures after surg...
- Perinatal complications in patients with unisutura...
- Effects of low-level laser therapy and platelet co...
- Development and Validation of a Modular Endoscopic...
- Diagnosis of Superior Semicircular Canal Dehiscenc...
- Significance of Endolymphatic Hydrops in Ears With...
- Vestibular Function in Adults With Epilepsy of Unk...
- Noonan Syndrome: An Underestimated Cause of Severe...
- Outcomes and Time to Emergence of Auditory Skills ...
- Audiologic Gain of Incus Short Process Vibroplasty...
- The Influence of Intraoperative Testing on Surgica...
- Reduction in Temporary and Permanent Audiological ...
- Role of Multislice CT Imaging in Predicting the Vi...
- Visual Processing Recruits the Auditory Cortices i...
- Can Preoperative CT Scans Be Used to Predict Facia...
- The Role of Preoperative Steroids in Atraumatic Co...
- Paediatric endonasal dacryocystorhinostomy using a...
- Effectiveness of Dietetic Consultations in Primary...
- Which patient with thyroid cancer deserves systemi...
- Expansion of blood IgG4+ Bcells, Th2 and Tregulato...
- Cardiac complexity and emotional dysregulation in ...
- Diagnostic implications of TERT promoter mutation ...
- Sarcomatoid carcinoma associated with small cell c...
- Spatial variation and toxicity assessment for heav...
- Guía de Práctica Clínica Para el Diagnóstico y Tra...
- Recombinant human IgG1 based Fc multimers, with li...
- IFC(EDITORIAL BOARD)
- DNA polymerase ι: the long and the short of it!
- A novel somatic JAK2 kinase-domain mutation in ped...
- Mentalizing regions represent distributed, continu...
- Decoding cognitive concepts from neuroimaging data...
- Suppression of irrelevant sounds during auditory w...
- Unidirectional brain to muscle connectivity reveal...
- MB-SWIFT functional MRI during deep brain stimulat...
- Simultaneous intrinsic signal imaging of auditory ...
- Differential expression of transforming growth fac...
- Evaluation of coronary artery disease as a risk fa...
- Coexistence of congenital left ventricular aneurys...
- Editorial Board
- Table of Contents
- Information for Authors
- Masthead
- Effects of transcranial direct current stimulation...
- Inter-hemispheric control of vestibular thresholds
- Brief rTMS delivered by H-Coil to a healthy volunt...
- Prevalence of high-risk human papillomavirus infec...
- Distribution and controls of heavy metals and orga...
- Mechanisms of Mixed Chimerism-Based Transplant Tol...
- Post-Translational Peptide Splicing and T Cell Res...
- Reproducibility and validity of the Italian versio...
- Coexistence of congenital left ventricular aneurys...
- Carapax Trionycis extracts inhibit fibrogenesis of...
- Berberine activates caspase-9/cytochrome c-mediate...
- Circadian variation in anticonvulsant activity of ...
- Up-regulation of HCRP1 inhibits proliferation and ...
- Phytanic acid induced neurological alterations in ...
- Nrf2 activation is required for curcumin to induce...
- Anti-photoaging potential of propolis extract in U...
- The prognostic landscape of tumor-infiltrating imm...
- Glycyrrhizic acid attenuates myocardial injury: In...
- Catalpol suppressed proliferation, growth and inva...
- VDAC1 deacetylation is involved in the protective ...
- Antidiabetic activity of hydro-alcoholic stem bark...
- Evaluation of dose calculations accuracy of a comm...
- A chemometric investigation of aromatic emission p...
- Nitrous acid in a street canyon environment: Sourc...
- The blue skies in Beijing during APEC 2014: A quan...
- Effect of relative humidity on O3 and NO2 oxidatio...
- Nano-sized and other improved reporters for Magnet...
- Experimental cocrystal screening and solution base...
- The Currarino Triad
- Iron deficiency anemia in children with cyanotic c...
-
▼
Αυγ 19
(179)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου